endothelial cell growth media microvascular 2 ecgm mv2  (PromoCell)

Journal: Bioactive Materials

Article Title: Ribbon-shaped microgels as bioinks for 3D bioprinting of anisotropic tissue structures

doi: 10.1016/j.bioactmat.2025.12.040

Figure Lengend Snippet: Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for endothelial cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.

Article Snippet: Human umbilical vein endothelial cells (HUVECs, Lonza) were cultured in Endothelial Cell Growth Media (R&D Systems).

Techniques: Staining

Journal: Biomaterials Science

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels

doi: 10.1039/d5bm01573a

Figure Lengend Snippet: Rat Schwann cell migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.

Article Snippet: For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium).

Techniques: Migration, Cell Characterization

Journal: Biomaterials Science

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels

doi: 10.1039/d5bm01573a

Figure Lengend Snippet: Rat Schwann cell morphology in magnetically templated hydrogels is influenced by channel diameter. Confocal fluorescent images of Schwann cells in hydrogels templated with (A, J) 32, (B, K) 62, and (C, L) 90 µm MAMs at day 1 (A–C) and day 3 (J–L). Top view of reconstructed surfaces using IMARIS in (D) 32, (E) 62, and (F) 90 µm MAM templated hydrogels at day 1 and day 3 (M–O). Side view of reconstructed surfaces using IMARIS in (G) 32, (H) 62, and (I) 90 µm MAM templated hydrogels at day 1 and day 3 (P–R). White bars at the top represent the respective MAM diameter size during templating for comparison of the cell structure.

Article Snippet: For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium).

Techniques: Comparison