Activity Assay:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Expressing:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Binding Assay:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Incubation:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Methylation:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Residue:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Western Blot:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Gene Expression:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Control:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Staining:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
WST-1 Assay:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Cytometry:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Knockdown:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Transfection:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Negative Control:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
In Vivo:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Real-time Polymerase Chain Reaction:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
MANN-WHITNEY:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
In Vitro:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
Quantitative RT-PCR:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
MTT Assay:Article Title: KMT2A degradation is observed in decitabine-responsive acute lymphoblastic leukemia cells.
Article Snippet: Human B—ALL cell lines SEM (RRID:CVCL_0095), RS4;11 (RRID:CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID:CVCL_0092) as well as human AML lines MV4;11 (RRID: CVCL_0064), and MOLM13 (RRID:CVCL_2119) were purchased from DSMZ (Braunschweig, Germany), and maintained at 37 °C and 5% CO2 in IMDM medium (SEM), Alpha MEM medium (RS4;11), or RPMI medium (REH, NALM-6, MV4;11, MOLM13) supplemented with 10% heat-inactivated fetal calf serum, and 100 μg mL 1 penicillin/streptomycin (all PAN—biotech, Aidenbach, Germany). .. M
Article Title: A novel and efficient tandem CD19- and CD22-directed CAR for B cell ALL.
Article Snippet: The B-ALL cell lines SEM and NALM6 were obtained from the DSMZ cell line bank (Braunschweig, Germany).. Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).Luciferase (Luc)/ GFP-expressing NALM6 cells were kindly provided by Prof. R.J. Brentjens (Memorial Sloan Kettering Cancer Center, NY, USA).
Article Title: Connectivity mapping identifies HDAC inhibitors for the treatment of t(4;11)-positive infant acute lymphoblastic leukemia.
Article Snippet: Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.Received 15 November 2010; revised 12 July 2011; accepted 9 August 2011; published online 21 October 2011 Correspondence: Dr RW Stam, Department of Pediatric Oncology/ Hematology, Erasmus Medical Center, Sophia Children’s Hospital, Dr Molewaterplein 50, Room: Ee15-14a, PO Box 2060, Rotterdam, Zuid-Holland, 3015 GE, Netherlands.. E-mail: r.stam@erasmusmc.nl Leukemia (2012) 26, 682–692 & 2012 Macmillan Publishers Limited All rights reserved 0887-6924/12 www.nature.com/leu t(4;11)-positive ALL cell line models The t(4;11)-positive precursor B-ALL cell lines SEM and RS4;11 were purchased from DSMZ (Braunschweig, Germany).. Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.Both cell lines were maintained as suspension cultures in RPMI 1640 with L-Alanyl-L-Glutamine (Invitrogen, Breda, the Netherlands) supplemented with 10% FCS (Integro, Zaandam, the Netherlands), 100 IU/ml penicillin, 100mg/ml streptomycin, and 0.125 mg/ml fungizone (Invitrogen) at 37 1C in humidified air containing 5% CO2.
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DSMZ is a verified supplier 
![Fig. 1. Decitabine (DEC)-induced effects on methyltransferase activity, gene and protein expression. (A) 3D crystal protein structure of DNMT1 (gray) in complex with DNA (red). The DNA-binding CXXC domain is colored in green. RCSB Protein data bank PDB identifier 3PTA [6,74]. (B) Crystal structure of the KMT2A CXXC domain (green) bound to human DNA (red). RCSB Protein data bank PDB identifier 2KKF [75,76]. (C) Amino acid (aa) sequences of the DNMT1 (top), and KMT2A (bottom) CXXC domains were retrieved from uniprot [77]. Shared amino acids were analyzed using BLASTP suite-2sequences [78] search, and depicted in the middle box. (D–G) Acute B-lymphoblastic leukemia cell lines <t>SEM,</t> <t>RS4;11,</t> REH, and NALM-6 were incubated with 1 μM DEC or vehicle substance (DMSO) for 72 h. (D) Influence on global methylation was analyzed by methylation-specific qPCR of the LINE-1 retrotransposon. n = 3, mean SD; paired t test. (E) Quantitative analysis of histone 3, lysine residue 4 trimethylation (H3K4me3) by western blot was used to determine the global transcription rate. n = 3, mean SD; ratio paired t test of absolute GAPDH-normalized signal intensities. (F) Probe-based gene expression analysis of DNMT1, and KMT2A. Both, the N-terminal CXXC-domain as well as the C-terminal SET domain have been analyzed. n = 3, mean SD; paired t test of ΔCt vs. GAPDH values. (G) Quantitative analysis of DNMT1, and KMT2A protein expression. n = 3, mean SD; ratio paired t test of absolute GAPDH-normalized signal intensities. *P < 0.05; **P < 0.01.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_4404/pm39754404/pm39754404__page5_image1.jpg)
