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    ATCC cell lines nccit
    Cell Lines Nccit, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+nccit/NCCIT/pmc12677466-268-0-3
    Average 95 stars, based on 263 article reviews
    cell lines nccit - by Bioz Stars, 2026-10
    95/100 stars

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    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in..

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC).. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30+ and CD30− Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Flow Cytometry:

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in..

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC).. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30+ and CD30− Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Positive Control:

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in..

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC).. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30+ and CD30− Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Microarray:

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in..

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC).. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30+ and CD30− Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Staining:

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in..

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC).. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30+ and CD30− Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Marker:

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera-2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.This study employed established human GCT cell lines, namely embryonal carcinoma (EC) cell lines Tera2, NTERA-2 and NCCIT, yolk sac tumor (YST) cell line NOY-1, teratocarcinoma (T/TC) cell line SuSa, and healthy human testicular fibroblasts Hs 1.Tes.. Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in..

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).The Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany).. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC).. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 or CD30, respectively.

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 + and CD30 – Embryonal Carcinoma via Antigen-dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o

    Article Title: CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30+ and CD30− Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions
    Article Snippet: Hodgkin’s lymphoma-derived cell line HDLM-2 was obtained from the German Collection of Cell Cultures (DMSZ, Braunschweig, Germany). .. The Burkitt’s lymphoma-derived cell line Raji, the EC-derived cell lines NCCIT, Tera-1, and Tera-2, the neuroblastoma-derived cell lines Lan-1 and SH-SY5Y, and the leukemia-derived cell line K562 were obtained from American Type Culture Collection (ATCC). .. K562 cells and Lan-1 cells were transduced with a retroviral vector encoding either human CD19 or CD30 to constitutively express CD19 o



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    Image Search Results


    a The relationship between stemness-associated genes and pseudotime. b Microscopic images of NCCIT spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images of Tcam-2 spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.

    Journal: Cell Death Discovery

    Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

    doi: 10.1038/s41420-025-02802-4

    Figure Lengend Snippet: a The relationship between stemness-associated genes and pseudotime. b Microscopic images of NCCIT spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images of Tcam-2 spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.

    Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

    Techniques: Knockdown, Expressing, Gene Expression, Over Expression

    a Confluence rate of Tcam-2 and NCCIT cells in wound healing assays (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.05, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.05; NCCIT: Ctrl vs DPPA4 -KD: P < 0.001, Ctrl vs PSMA7 -KD: P < 0.001, Ctrl vs PAGE5 -OE: P < 0.001, Ctrl vs SAT1 -OE: P < 0.05). b Box plot showing proliferation scores across different cell types. c GSEA plot showing enrichment of MYC target v1 gene signature in embryonal carcinoma cells compared to seminoma cells. d GSEA plot showing enrichment of MYC target v2 gene signature in embryonal carcinoma cells compared to seminoma cells. e GSEA plot showing enrichment of mTORC1 signaling gene signature in embryonal carcinoma cells compared to seminoma cells. f GSEA plot showing enrichment of MYC target v1 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. g GSEA plot showing enrichment of MYC target v2 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. h GSEA plot showing enrichment of mTORC1 signaling gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. i Growth curves of Tcam-2 and NCCIT cells (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.0001, Ctrl vs SAT1 -OE: P < 0.0001; NCCIT: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.01).

    Journal: Cell Death Discovery

    Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

    doi: 10.1038/s41420-025-02802-4

    Figure Lengend Snippet: a Confluence rate of Tcam-2 and NCCIT cells in wound healing assays (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.05, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.05; NCCIT: Ctrl vs DPPA4 -KD: P < 0.001, Ctrl vs PSMA7 -KD: P < 0.001, Ctrl vs PAGE5 -OE: P < 0.001, Ctrl vs SAT1 -OE: P < 0.05). b Box plot showing proliferation scores across different cell types. c GSEA plot showing enrichment of MYC target v1 gene signature in embryonal carcinoma cells compared to seminoma cells. d GSEA plot showing enrichment of MYC target v2 gene signature in embryonal carcinoma cells compared to seminoma cells. e GSEA plot showing enrichment of mTORC1 signaling gene signature in embryonal carcinoma cells compared to seminoma cells. f GSEA plot showing enrichment of MYC target v1 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. g GSEA plot showing enrichment of MYC target v2 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. h GSEA plot showing enrichment of mTORC1 signaling gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. i Growth curves of Tcam-2 and NCCIT cells (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.0001, Ctrl vs SAT1 -OE: P < 0.0001; NCCIT: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.01).

    Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

    Techniques: