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human cervical cancer cell line hela  (ATCC)


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    ATCC human cervical cancer cell line hela
    Human Cervical Cancer Cell Line Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hela/HeLa/pm42882443-114-17-23
    Average 99 stars, based on 24636 article reviews
    human cervical cancer cell line hela - by Bioz Stars, 2026-11
    99/100 stars

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    other:

    Article Title: Development of a p62 biodegrader for autophagy targeted degradation.
    Article Snippet: Doxycyclin-Hyclat (D5207, Merck) was dissolved at 1mgmL−1 in deionized water.

    Cell Culture:

    Article Title: Strategic engineering of DNA aptamers as precision modulators in CRISPR-driven oncogene control
    Article Snippet: .. Cell lines and cell culture The cell lines Hela, T24, 5637, UMUC-3, and J82 used in this study were sourced from the American Type Culture Collection, located in Manassas, VA, USA. ..

    Article Title: USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progression.
    Article Snippet: .. MATERIALS AND METHODS Cell culture and transfection The cell lines HeLa, HepG2, and HCT116 were obtained from ATCC, while HEK293 and HEK293T cell lines were sourced from the Cell Bank at the Shanghai Institute of Biochemistry and Cell Biology (SIBCB), Chinese Academy of Sciences. .. HeLa, HepG2, HEK293, and HEK293T cells were cultured in DMEM (Gibco, C11995500BT), whereas HCT116 cells were maintained in McCoy’s 5A medium.

    Article Title: STX1A localizes to the lysosome and controls its exocytosis
    Article Snippet: Lysosome exocytosis is one of the critical functions of lysosomes in maintaining cellular homeostasis and plasma membrane repair.. At the basal level, the SNAREs regulating the lysosome fusion with the cell surface have been poorly defined.. Here, we identified a Qa-SNARE STX1A, localized majorly to lysosomes and a cohort to the plasma membrane in HeLa cells.

    Transfection:

    Article Title: USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progression.
    Article Snippet: .. MATERIALS AND METHODS Cell culture and transfection The cell lines HeLa, HepG2, and HCT116 were obtained from ATCC, while HEK293 and HEK293T cell lines were sourced from the Cell Bank at the Shanghai Institute of Biochemistry and Cell Biology (SIBCB), Chinese Academy of Sciences. .. HeLa, HepG2, HEK293, and HEK293T cells were cultured in DMEM (Gibco, C11995500BT), whereas HCT116 cells were maintained in McCoy’s 5A medium.

    Article Title: STX1A localizes to the lysosome and controls its exocytosis
    Article Snippet: Lysosome exocytosis is one of the critical functions of lysosomes in maintaining cellular homeostasis and plasma membrane repair.. At the basal level, the SNAREs regulating the lysosome fusion with the cell surface have been poorly defined.. Here, we identified a Qa-SNARE STX1A, localized majorly to lysosomes and a cohort to the plasma membrane in HeLa cells.

    Transduction:

    Article Title: STX1A localizes to the lysosome and controls its exocytosis
    Article Snippet: Lysosome exocytosis is one of the critical functions of lysosomes in maintaining cellular homeostasis and plasma membrane repair.. At the basal level, the SNAREs regulating the lysosome fusion with the cell surface have been poorly defined.. Here, we identified a Qa-SNARE STX1A, localized majorly to lysosomes and a cohort to the plasma membrane in HeLa cells.



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    Image Search Results


    Screening and selection of sgRNAs targeting candidate lectins in HeLa S3 cells. A. Schematic of the sgRNA screening workflow. HeLa S3 cells were nucleofected with pre-assembled Cas9–sgRNA RNPs targeting CLEC4E, CLEC6A, CD209, SELL, or SIGLEC9, with CD14 included as a technical reference control. Genomic editing was assessed three days after nucleofection by Sanger sequencing and ICE analysis to support selection of sgRNAs for subsequent evaluation in primary human monocytes. B. Schematic representation of the lectins genomic locus showing annotated RefSeq transcript isoforms, exon organization (blue), and the position of the targeting sgRNAs (orange). C. Predicted frameshift frequencies determined by ICE analysis following individual sgRNA delivery in HeLa S3 cells. Hatched bars indicate the sgRNA selected for each target for subsequent evaluation in primary human monocytes. Data represent a single technical screening experiment.

    Journal: bioRxiv

    Article Title: CRISPR–Cas9 Editing of Myeloid Lectins Reveals Target-Dependent Transcriptional Remodeling in Tumor-Associated Macrophages

    doi: 10.64898/2026.10.02.756241

    Figure Lengend Snippet: Screening and selection of sgRNAs targeting candidate lectins in HeLa S3 cells. A. Schematic of the sgRNA screening workflow. HeLa S3 cells were nucleofected with pre-assembled Cas9–sgRNA RNPs targeting CLEC4E, CLEC6A, CD209, SELL, or SIGLEC9, with CD14 included as a technical reference control. Genomic editing was assessed three days after nucleofection by Sanger sequencing and ICE analysis to support selection of sgRNAs for subsequent evaluation in primary human monocytes. B. Schematic representation of the lectins genomic locus showing annotated RefSeq transcript isoforms, exon organization (blue), and the position of the targeting sgRNAs (orange). C. Predicted frameshift frequencies determined by ICE analysis following individual sgRNA delivery in HeLa S3 cells. Hatched bars indicate the sgRNA selected for each target for subsequent evaluation in primary human monocytes. Data represent a single technical screening experiment.

    Article Snippet: BT474 and HeLa S3 cell lines were acquired from ATCC and cultured in RPMI 1640 or DMEM medium (Gibco), respectively, both supplemented with 10% (v/v) FBS (Gibco).

    Techniques: Screening, Selection, Control, DNA Sequencing, Analysis