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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+synthesis/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11036 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    Article Title: Platinum-doped emodin carbon dots mitigate sepsis-induced lung injury by targeting the gut-lung axis
    Article Snippet: Total RNA was isolated using RNAfast200 reagent, followed by cDNA synthesis with PrimeScript RT Reagent Kit (TaKaRa RR037A; integrates gDNA Eraser).

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3k27Me3 directed transcriptional repression.
    Article Snippet: Reverse transcription of RNA was performed using PrimeScript RT Master Mix kit for cDNA synthesis (TaKaRa, RR036A).

    Article Title: Lacticaseibacillus paracasei CM6 postbiotics alleviate SO/SP-induced hepatic lipotoxicity in HepG2 cells: Insights into lipid-redox homeostasis, glutamate metabolism and PLA2G4A-dependent lysophospholipid remodeling.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan).

    Article Title: HIPK2-and IKKβ-dependent phosphorylation stabilizes TAp63α during the oocyte DNA damage response
    Article Snippet: RNA concentration was then measured, and cDNA synthesis was conducted according to the manufacturer’s protocol (Cat No. 6110a, Takara).

    Article Title: Canonical NF-κB regulator CYLD mutations reinforce RelB mediated disease pathogenesis in Multiple Myeloma
    Article Snippet: For gene expression studies, cells were harvested in RNAiso Plus reagent (Takara; Cat # 9108) and total RNA from the cells were isolated and followed by cDNA synthesis (Takara; Cat # 6110A) for qRT-PCR based gene expression studies (see Appendix Table S1 for primer descriptions).

    Article Title: Integrative bulk and single-cell transcriptomic analysis reveals COL1A2-driven ECM remodeling and focal adhesion signaling associated with the transition from non-muscle-invasive to muscle-invasive bladder cancer
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Vazyme, China), followed by cDNA synthesis with reverse transcriptase (PrimeScript RT Kit, Takara).

    cDNA Synthesis:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    Article Title: Platinum-doped emodin carbon dots mitigate sepsis-induced lung injury by targeting the gut-lung axis
    Article Snippet: Total RNA was isolated using RNAfast200 reagent, followed by cDNA synthesis with PrimeScript RT Reagent Kit (TaKaRa RR037A; integrates gDNA Eraser).

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3k27Me3 directed transcriptional repression.
    Article Snippet: Reverse transcription of RNA was performed using PrimeScript RT Master Mix kit for cDNA synthesis (TaKaRa, RR036A).

    Article Title: Lacticaseibacillus paracasei CM6 postbiotics alleviate SO/SP-induced hepatic lipotoxicity in HepG2 cells: Insights into lipid-redox homeostasis, glutamate metabolism and PLA2G4A-dependent lysophospholipid remodeling.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan).

    Article Title: HIPK2-and IKKβ-dependent phosphorylation stabilizes TAp63α during the oocyte DNA damage response
    Article Snippet: RNA concentration was then measured, and cDNA synthesis was conducted according to the manufacturer’s protocol (Cat No. 6110a, Takara).

    Article Title: Canonical NF-κB regulator CYLD mutations reinforce RelB mediated disease pathogenesis in Multiple Myeloma
    Article Snippet: For gene expression studies, cells were harvested in RNAiso Plus reagent (Takara; Cat # 9108) and total RNA from the cells were isolated and followed by cDNA synthesis (Takara; Cat # 6110A) for qRT-PCR based gene expression studies (see Appendix Table S1 for primer descriptions).

    Article Title: Integrative bulk and single-cell transcriptomic analysis reveals COL1A2-driven ECM remodeling and focal adhesion signaling associated with the transition from non-muscle-invasive to muscle-invasive bladder cancer
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Vazyme, China), followed by cDNA synthesis with reverse transcriptase (PrimeScript RT Kit, Takara).

    Gene Expression:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    Article Title: Platinum-doped emodin carbon dots mitigate sepsis-induced lung injury by targeting the gut-lung axis
    Article Snippet: Total RNA was isolated using RNAfast200 reagent, followed by cDNA synthesis with PrimeScript RT Reagent Kit (TaKaRa RR037A; integrates gDNA Eraser).

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3k27Me3 directed transcriptional repression.
    Article Snippet: Reverse transcription of RNA was performed using PrimeScript RT Master Mix kit for cDNA synthesis (TaKaRa, RR036A).

    Article Title: Lacticaseibacillus paracasei CM6 postbiotics alleviate SO/SP-induced hepatic lipotoxicity in HepG2 cells: Insights into lipid-redox homeostasis, glutamate metabolism and PLA2G4A-dependent lysophospholipid remodeling.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan).

    Article Title: HIPK2-and IKKβ-dependent phosphorylation stabilizes TAp63α during the oocyte DNA damage response
    Article Snippet: RNA concentration was then measured, and cDNA synthesis was conducted according to the manufacturer’s protocol (Cat No. 6110a, Takara).

    Article Title: Canonical NF-κB regulator CYLD mutations reinforce RelB mediated disease pathogenesis in Multiple Myeloma
    Article Snippet: For gene expression studies, cells were harvested in RNAiso Plus reagent (Takara; Cat # 9108) and total RNA from the cells were isolated and followed by cDNA synthesis (Takara; Cat # 6110A) for qRT-PCR based gene expression studies (see Appendix Table S1 for primer descriptions).

    Article Title: Integrative bulk and single-cell transcriptomic analysis reveals COL1A2-driven ECM remodeling and focal adhesion signaling associated with the transition from non-muscle-invasive to muscle-invasive bladder cancer
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Vazyme, China), followed by cDNA synthesis with reverse transcriptase (PrimeScript RT Kit, Takara).

    Isolation:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    Article Title: Platinum-doped emodin carbon dots mitigate sepsis-induced lung injury by targeting the gut-lung axis
    Article Snippet: Total RNA was isolated using RNAfast200 reagent, followed by cDNA synthesis with PrimeScript RT Reagent Kit (TaKaRa RR037A; integrates gDNA Eraser).

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3k27Me3 directed transcriptional repression.
    Article Snippet: Reverse transcription of RNA was performed using PrimeScript RT Master Mix kit for cDNA synthesis (TaKaRa, RR036A).

    Article Title: Lacticaseibacillus paracasei CM6 postbiotics alleviate SO/SP-induced hepatic lipotoxicity in HepG2 cells: Insights into lipid-redox homeostasis, glutamate metabolism and PLA2G4A-dependent lysophospholipid remodeling.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan).

    Article Title: HIPK2-and IKKβ-dependent phosphorylation stabilizes TAp63α during the oocyte DNA damage response
    Article Snippet: RNA concentration was then measured, and cDNA synthesis was conducted according to the manufacturer’s protocol (Cat No. 6110a, Takara).

    Article Title: Canonical NF-κB regulator CYLD mutations reinforce RelB mediated disease pathogenesis in Multiple Myeloma
    Article Snippet: For gene expression studies, cells were harvested in RNAiso Plus reagent (Takara; Cat # 9108) and total RNA from the cells were isolated and followed by cDNA synthesis (Takara; Cat # 6110A) for qRT-PCR based gene expression studies (see Appendix Table S1 for primer descriptions).

    Article Title: Integrative bulk and single-cell transcriptomic analysis reveals COL1A2-driven ECM remodeling and focal adhesion signaling associated with the transition from non-muscle-invasive to muscle-invasive bladder cancer
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Vazyme, China), followed by cDNA synthesis with reverse transcriptase (PrimeScript RT Kit, Takara).

    Quantitative RT-PCR:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    Article Title: Platinum-doped emodin carbon dots mitigate sepsis-induced lung injury by targeting the gut-lung axis
    Article Snippet: Total RNA was isolated using RNAfast200 reagent, followed by cDNA synthesis with PrimeScript RT Reagent Kit (TaKaRa RR037A; integrates gDNA Eraser).

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3k27Me3 directed transcriptional repression.
    Article Snippet: Reverse transcription of RNA was performed using PrimeScript RT Master Mix kit for cDNA synthesis (TaKaRa, RR036A).

    Article Title: Lacticaseibacillus paracasei CM6 postbiotics alleviate SO/SP-induced hepatic lipotoxicity in HepG2 cells: Insights into lipid-redox homeostasis, glutamate metabolism and PLA2G4A-dependent lysophospholipid remodeling.
    Article Snippet: Genomic DNA was removed, and cDNA synthesis was performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan).

    Article Title: HIPK2-and IKKβ-dependent phosphorylation stabilizes TAp63α during the oocyte DNA damage response
    Article Snippet: RNA concentration was then measured, and cDNA synthesis was conducted according to the manufacturer’s protocol (Cat No. 6110a, Takara).

    Article Title: Canonical NF-κB regulator CYLD mutations reinforce RelB mediated disease pathogenesis in Multiple Myeloma
    Article Snippet: For gene expression studies, cells were harvested in RNAiso Plus reagent (Takara; Cat # 9108) and total RNA from the cells were isolated and followed by cDNA synthesis (Takara; Cat # 6110A) for qRT-PCR based gene expression studies (see Appendix Table S1 for primer descriptions).

    Article Title: Integrative bulk and single-cell transcriptomic analysis reveals COL1A2-driven ECM remodeling and focal adhesion signaling associated with the transition from non-muscle-invasive to muscle-invasive bladder cancer
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Vazyme, China), followed by cDNA synthesis with reverse transcriptase (PrimeScript RT Kit, Takara).



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