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human 1 cdna microarray slides  (Agilent technologies)


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    Agilent technologies human 1 cdna microarray slides
    Human 1 Cdna Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+slide/pmc02648774-120-9-14
    Average 90 stars, based on 1 article reviews
    human 1 cdna microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Host Transcript Accumulation during Lytic KSHV Infection Reveals Several Classes of Host Responses
    Article Snippet: Washed arrays were scanned using the 48-slide DNA Microarray Scanner (Agilent) and feature intensities extracted using Feature Extraction Software version 8.5 (Agilent).

    Microarray:

    Article Title: Overexpression of exogenous biuret hydrolase in rice plants confers tolerance to biuret toxicity
    Article Snippet: Total RNA was extracted from 3‐ and 5‐day‐old rice suspension cells grown in the media supplemented with 0 or 0.3 mmol/L biuret using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). .. Two‐color microarray analysis with two biological replicates was performed using Agilent Rice Oligo DNA Microarray 4x44K slide (Agilent, Santa Clara, CA, USA) according to the manufacturer's instructions to estimate the ratio of transcript abundance between the treatments at each culture period. .. Cyanine 3 or cyanine 5 labeled antisense cRNA was synthesized from the total RNA sample using Agilent Quick Amp Labeling Kit and hybridized to the microarray slide at 65°C for 17 hr using Agilent Gene Expression Hybridization Kit.

    Article Title: Transcriptomic analysis reveals distinct adaptive molecular mechanism in the hippocampal CA3 from rats susceptible or not-susceptible to hyperthermia-induced seizures.
    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:10265 | https://doi.org/10.1038/s41598-023-37535-w dye Cy3 followed the manufacturer’s protocols (One-Color Microarray-Based Gene Expression Analysis-Quick Amp Labeling and miRNA Complete Labeling and Hyb Kit, Agilent Technologies). .. A subset of 58 RNA samples was used for miRNA expression analysis, using the whole rat miRNA 8x15K oligonucleotide microarrays (Rat miRNA Microarray slide, G4471A-070154, Agilent Technologies), containing probes for 758 rat miRNAs based on miRBase database (release 21.0). .. The images were captured by the reader Agilent Bundle according to the parameters recommended for bioarrays and extracted by Agilent Feature Extraction software version 11.5.1.1 (https:// www. agile nt. com/) for both gene and miRNA expression.

    Article Title: Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress
    Article Snippet: .. After washing, slide image files were produced by a DNA microarray scanner (G2505B; Agilent Technologies). .. Signal intensities of Cy3 and Cy5 were extracted from the image files and normalized to remove the dye effect in signal intensity by rank consistency and the LOWESS method, processed by Feature Extraction version 9.5 (Agilent Technologies).

    Article Title: Relationship between Symptoms and Gene Expression Induced by the Infection of Three Strains of Rice dwarf virus
    Article Snippet: .. After being washed, the slide image files were produced by the DNA microarray scanner (G2505B; Agilent Technologies, Santa Clara, CA, USA). .. Signal intensities of Cy3 and Cy5 were extracted from the image files and normalized in each array by Feature Extraction version 9.5 (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report
    Article Snippet: .. Molecular karyotyping was performed using commercially available high resolution 244K 60-mer oligonucleotide microarray slide (Human Genome CGH Microarray 244A Kit, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer's protocol. ..

    Article Title: The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germination
    Article Snippet: The labeled cRNA was fragmented and hybridized to Agilent Arabidopsis 4 Oligo Microarrays (G2519F) for 17 h at 65 oC. .. After hybridization on 4 x 44K array slide, the arrays were washed and scanned by Agilent DNA Microarray Scanner (G2505B) according to one-color methods. .. Signal intensities were measured by Feature Extraction Software 11.5.1.1 (Agilent) and data analysis were performed by Gene Spring (Agilent) and R software.

    Expressing:

    Article Title: Transcriptomic analysis reveals distinct adaptive molecular mechanism in the hippocampal CA3 from rats susceptible or not-susceptible to hyperthermia-induced seizures.
    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:10265 | https://doi.org/10.1038/s41598-023-37535-w dye Cy3 followed the manufacturer’s protocols (One-Color Microarray-Based Gene Expression Analysis-Quick Amp Labeling and miRNA Complete Labeling and Hyb Kit, Agilent Technologies). .. A subset of 58 RNA samples was used for miRNA expression analysis, using the whole rat miRNA 8x15K oligonucleotide microarrays (Rat miRNA Microarray slide, G4471A-070154, Agilent Technologies), containing probes for 758 rat miRNAs based on miRBase database (release 21.0). .. The images were captured by the reader Agilent Bundle according to the parameters recommended for bioarrays and extracted by Agilent Feature Extraction software version 11.5.1.1 (https:// www. agile nt. com/) for both gene and miRNA expression.

    Produced:

    Article Title: Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress
    Article Snippet: .. After washing, slide image files were produced by a DNA microarray scanner (G2505B; Agilent Technologies). .. Signal intensities of Cy3 and Cy5 were extracted from the image files and normalized to remove the dye effect in signal intensity by rank consistency and the LOWESS method, processed by Feature Extraction version 9.5 (Agilent Technologies).

    Article Title: Relationship between Symptoms and Gene Expression Induced by the Infection of Three Strains of Rice dwarf virus
    Article Snippet: .. After being washed, the slide image files were produced by the DNA microarray scanner (G2505B; Agilent Technologies, Santa Clara, CA, USA). .. Signal intensities of Cy3 and Cy5 were extracted from the image files and normalized in each array by Feature Extraction version 9.5 (Agilent Technologies, Santa Clara, CA, USA).

    Incubation:

    Article Title: Genetic testing for hearing loss in the United States should include deletion/duplication analysis for the deafness/infertility locus at 15q15.3
    Article Snippet: .. Each mixture was then applied to an Agilent 4 × 180 K oligonucleotide array CGH slide and incubated while rotating at 65°C for 24 hours. .. Slides were washed and scanned on a G2565CA Microarray Scanner System (Agilent Technologies Inc., Santa Clara, CA).

    Hybridization:

    Article Title: The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germination
    Article Snippet: The labeled cRNA was fragmented and hybridized to Agilent Arabidopsis 4 Oligo Microarrays (G2519F) for 17 h at 65 oC. .. After hybridization on 4 x 44K array slide, the arrays were washed and scanned by Agilent DNA Microarray Scanner (G2505B) according to one-color methods. .. Signal intensities were measured by Feature Extraction Software 11.5.1.1 (Agilent) and data analysis were performed by Gene Spring (Agilent) and R software.



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    Image Search Results


    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Journal: Scientific Reports

    Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

    doi: 10.1038/srep20395

    Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

    Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining