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cdna ends  (TaKaRa)


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    TaKaRa cdna ends
    Cdna Ends, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+ends/SMARTer+RACE+5%E2%80%99%2F3%E2%80%99+Kit/pm42048749-45-6-17
    Average 99 stars, based on 2962 article reviews
    cdna ends - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Rapid Amplification of cDNA Ends:

    Article Title: Kdm4dl is expressed in adult mouse testis and encodes a functional KDM4D-like protein.
    Article Snippet: .. 5′ and 3′ rapid amplification of cDNA ends (RACE) was performed using the SMARTer RACE 5’/3′ Kit (Takara; Z4858 N) according to the manufacturer's protocol. ..

    Article Title: Comprehensive Genomic Analysis Reveals the Class II Diterpene Cyclases in Dodonaea viscosa.
    Article Snippet: Dodonaea viscosa is known for its rich arsenal of phytochemicals and has been traditionally used as a folk medicine.. Decades of research have targeted isolation of the potent bioactives, including the unique clerodane and labdane diterpenoids identified in this plant.. Growing interest in these bioactive compounds from D. viscosa Jacq. provides impetus towards elucidation of their biosynthetic pathway to enable the potential for scalable production via synthetic biology.

    Article Title: Tumor Extracellular Vesicles lncOSLMT Drives Lung Inflammatory Premetastatic Niche Formation in Osteosarcoma via m 6 A‐Dependent hnRNPA2B1/COX‐2 Axis
    Article Snippet: .. To obtain the full‐length sequence of the target lncRNA, 5’ and 3’ rapid amplification of cDNA ends (RACE) was performed using the SMARTer RACE 5’/3’ Kit (Takara, USA) according to the manufacturer's instructions. .. Total RNA was extracted using TRIzol (Invitrogen) and reverse‐transcribed to generate 5’ and 3’ RACE‐ready cDNA.

    Article Title: Overexpression of CdZFP3, a Cynodon dactylon zinc finger protein gene, enhanced salt tolerance in Arabidopsis thaliana
    Article Snippet: .. To generate the full-length CdZFP3 gene, 5’- and 3’- rapid amplification of cDNA ends (RACE) were performed using the SMARTer RACE 5’/3’ Kit (Clontech, CA, USA). ..

    Article Title: Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress
    Article Snippet: We employed Primer Premier v.5.0 software (Premier Biosoft, San Francisco, CA, USA) [ ] to design the primers based on verified M. japonicus genome sequences. .. Subsequently, the full-length cDNA was obtained by rapid amplification of cDNA ends (RACE) employing a SMARTer RACE 5’/3’ Kit (Takara, Shiga, Japan). shows the primer sequences. .. The MjHSP90 amplicons were sequenced by Sangon Biotech following ligation into the pMD18-T vector (Takara).

    Article Title: Tumor Extracellular Vesicles lncOSLMT Drives Lung Inflammatory Premetastatic Niche Formation in Osteosarcoma via m 6 A-Dependent hnRNPA2B1/COX-2 Axis.
    Article Snippet: .. To obtain the full-length sequence of the target lncRNA, 5’ and 3’ rapid amplification of cDNA ends (RACE) was performed using the SMARTer RACE 5’/3’ Kit (Takara, USA) according to the manufacturer’s instructions. .. Total RNA was extracted using TRIzol (Invitrogen) and reverse-transcribed to generate 5’ and 3’ RACE-ready cDNA.

    Sequencing:

    Article Title: Tumor Extracellular Vesicles lncOSLMT Drives Lung Inflammatory Premetastatic Niche Formation in Osteosarcoma via m 6 A‐Dependent hnRNPA2B1/COX‐2 Axis
    Article Snippet: .. To obtain the full‐length sequence of the target lncRNA, 5’ and 3’ rapid amplification of cDNA ends (RACE) was performed using the SMARTer RACE 5’/3’ Kit (Takara, USA) according to the manufacturer's instructions. .. Total RNA was extracted using TRIzol (Invitrogen) and reverse‐transcribed to generate 5’ and 3’ RACE‐ready cDNA.

    Article Title: Tumor Extracellular Vesicles lncOSLMT Drives Lung Inflammatory Premetastatic Niche Formation in Osteosarcoma via m 6 A-Dependent hnRNPA2B1/COX-2 Axis.
    Article Snippet: .. To obtain the full-length sequence of the target lncRNA, 5’ and 3’ rapid amplification of cDNA ends (RACE) was performed using the SMARTer RACE 5’/3’ Kit (Takara, USA) according to the manufacturer’s instructions. .. Total RNA was extracted using TRIzol (Invitrogen) and reverse-transcribed to generate 5’ and 3’ RACE-ready cDNA.

    Amplification:

    Article Title: Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae).
    Article Snippet: 5 μg of DNA-free total RNA was used to isolate mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490, New England Biolabs Inc., Ipswich, MA, USA). .. Both 5’- and 3’-rapid amplification of cDNA ends (RACE) was performed using SMARTer® RACE 5’/3’ Kit (Takara Bio USA, Inc., Mountain View, CA, USA) following the manufacturer’s instructions with primers P2125 and P2126. .. Cycler conditions were as follows: 5 cycles 94 °C for 30 s, 72 °C for 3 min, 5 cycles 94 °C for 30 s, 70 °C for 30 s, 72 °C for 3 min, 25 cycles 94 °C for 30 s, 65 °C for 30 s, 72 °C for 3 min. PCR products were analyzed via gel electrophoresis, extracted using Zymoclean Gel DNA Recovery Kit (Zymo Research Europe GmbH, Freiburg, Germany), cloned into the linearized pRACE vector (Takara Bio USA, Inc.), and transformed into XL1-Blue MR Supercompetent cells (E. coli (mcrA)183 (mcrCB-hsdSMRmrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F proAB lacIqZ M15 Tn10 (Tetr)]; Agilent Technologies, Santa Clara, CA, USA).

    other:

    Article Title: A hexamer tandem repeat RNA embedded within an SVA retrotransposon drives R-loop formation and neurodegeneration.
    Article Snippet: The nested PCR products were purified using a DNA clean/concentrator kit (Zymo Research) following the manufacturer’s instructions.



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    (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).
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    The nucleotide sequence and translated ORF amino acids of a <t>cDNA</t> encoding a novel peptide from L. caerulea skin secretions. The nucleotide sequence of the signal peptide is double-underlined. The nucleotide sequence of mature peptide Caerin 1.1-LC is single-underlined and marked in yellow. The stop codon is indicated by an asterisk.
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    Image Search Results


    (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).

    Journal: bioRxiv

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    doi: 10.64898/2026.04.24.720579

    Figure Lengend Snippet: (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).

    Article Snippet: Five-prime rapid amplification of cDNA end (5’ RACE) analyses of RNAs purified from mutant virus particles revealed multiple RNA variants with 5’ terminal sequences differing from the plasmid used for producing the particles.

    Techniques: Transfection, Plasmid Preparation, Mutagenesis, Purification, Virus, Clone Assay, Reverse Transcription

    (A) Nucleotide sequences of the CCC-AAA, CCC-GGG and CCC-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the CCC-AAA (left panels; n=38 clones), the CCC-GGG (center panels; n=40 clones) and the CCC-TTT mutant virus (right panels; n=39 clones).

    Journal: bioRxiv

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    doi: 10.64898/2026.04.24.720579

    Figure Lengend Snippet: (A) Nucleotide sequences of the CCC-AAA, CCC-GGG and CCC-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the CCC-AAA (left panels; n=38 clones), the CCC-GGG (center panels; n=40 clones) and the CCC-TTT mutant virus (right panels; n=39 clones).

    Article Snippet: Five-prime rapid amplification of cDNA end (5’ RACE) analyses of RNAs purified from mutant virus particles revealed multiple RNA variants with 5’ terminal sequences differing from the plasmid used for producing the particles.

    Techniques: Purification, Clone Assay, Mutagenesis, Virus

    (A) Nucleotide sequences of the GGG-AAA, GGG-CCC and GGG-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B-D) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the GGG-AAA (B; n=43 clones), the GGG-CCC (C; n=40 clones) and the GGG-TTT mutant virus (D; n=38 clones).

    Journal: bioRxiv

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    doi: 10.64898/2026.04.24.720579

    Figure Lengend Snippet: (A) Nucleotide sequences of the GGG-AAA, GGG-CCC and GGG-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B-D) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the GGG-AAA (B; n=43 clones), the GGG-CCC (C; n=40 clones) and the GGG-TTT mutant virus (D; n=38 clones).

    Article Snippet: Five-prime rapid amplification of cDNA end (5’ RACE) analyses of RNAs purified from mutant virus particles revealed multiple RNA variants with 5’ terminal sequences differing from the plasmid used for producing the particles.

    Techniques: Purification, Clone Assay, Mutagenesis, Virus

    The nucleotide sequence and translated ORF amino acids of a cDNA encoding a novel peptide from L. caerulea skin secretions. The nucleotide sequence of the signal peptide is double-underlined. The nucleotide sequence of mature peptide Caerin 1.1-LC is single-underlined and marked in yellow. The stop codon is indicated by an asterisk.

    Journal: Pharmaceutics

    Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

    doi: 10.3390/pharmaceutics17111500

    Figure Lengend Snippet: The nucleotide sequence and translated ORF amino acids of a cDNA encoding a novel peptide from L. caerulea skin secretions. The nucleotide sequence of the signal peptide is double-underlined. The nucleotide sequence of mature peptide Caerin 1.1-LC is single-underlined and marked in yellow. The stop codon is indicated by an asterisk.

    Article Snippet: Subsequently, a cDNA library was constructed with the SMARTer ® Rapid Amplification of cDNA Ends (RACE) 5′/3′ Kit (Clontech, Mountain View, CA, USA).

    Techniques: Sequencing