anti cdkn1a  (Bioss)

Journal: Journal of Inflammation Research

Article Title: Fatty Acid Transporter CD36 Promotes Ox-LDL-Induced Senescence of Vascular Endothelial Cells in Preeclampsia

doi: 10.2147/JIR.S538337

Figure Lengend Snippet: PE placentas showed significant senescence-related phenotypes. ( A and B ) Western blot analysis of CDKN2A, CDKN1A, and TP53 protein expression in PE (n=4) and NC groups (n=4) ( A ) and quantitative results (B); unpaired t test. ( C – E ) Detecting protein expression level of inflammatory factors in the PE (n=8) and NC placentas (n=8) by Elisa, including IL-6 (C), CXCL-8 ( D ) and TNF-α (E); unpaired t test. ( G – I ) Maternal plasma IL-6 (F), CXCL-8 ( G ) and TNF-α ( H ) levels in the NC (n=18) and PE group (n=18); unpaired t test. ( I ) Result of GSEA. Three senescence-related gene sets were significantly activated in the PE placenta (P-value < 0.05, FDR q-value < 0.25). The data are expressed as the mean ± SD of three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After blocking with 5% BSA and washing with TBST, the membrane was incubated overnight with the diluted primary antibody, including CD36 (1:1000, 18836-1-AP, Proteintech, China), ox-LDL (1:3000, PAA527Hu08, Cloud-Clone, China), CDKN2A (1:4000, 10883-1-AP, Proteintech, China), CDKN1A (1:2000, 10355-1-AP, Proteintech, China), TP53 (1:20,000, 10442-1-AP, Proteintech, China).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Cell Death Discovery

Article Title: Bisphenol-A disrupts mitochondrial functionality leading to senescence and apoptosis in human amniotic mesenchymal stromal cells

doi: 10.1038/s41420-025-02620-8

Figure Lengend Snippet: Expression of p53, p21 and p27 cell-cycle regulating genes was analysed by RT-PCR 24 h after exposure to increasing BPA concentrations (0.05, 0.1, 0.2, 0.3, 0.35 and 0.4 μM). Results are presented as fold-change relative to control conditions (MetOH) ( A ). Furthermore, p21 protein expression and its nuclear translocation in hAMSC were assessed 24 h after exposure to increasing BPA concentrations (0.1, 0.2, 0.3, and 0.4 μM) using immunofluorescence analysis. Pictures were acquired at ×20 magnification (scale bar, 50 μm) ( B ). p21-positive cells were identified by a rosy-red signal, while nuclei were stained with DAPI (blue). The total number of p21 positive cells was quantified and is reported in ( C ). Fluorescence intensity of p21, measured as Normalized Integrated Density, is presented in ( D ). Histograms represent the mean values ± standard deviation from n = 4 ( A ) and n = 3 ( B ) independent experiments. Statistical analysis was performed versus the control condition represented by the MetOH: p < 0.01(**), p < 0.001(***), p < 0.0001(****).

Article Snippet: Subsequently, cells were washed three times with Tris Buffered Saline (TBS) for 5 min each. hAMSC were incubated with p21 primary antibody (Waf1/Cip1/CDKN1A p21, sc-6246, Santa Cruz Biotechnology, Texas, USA), diluted 1:100 in normal goat serum (NGS, Invitrogen, Waltham USA, #10000C) and incubated overnight at 4 °C in the dark.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Translocation Assay, Immunofluorescence, Staining, Fluorescence, Standard Deviation

Journal: Cell Death Discovery

Article Title: Bisphenol-A disrupts mitochondrial functionality leading to senescence and apoptosis in human amniotic mesenchymal stromal cells

doi: 10.1038/s41420-025-02620-8

Figure Lengend Snippet: Alteration in mitochondrial function, evidenced by enhanced production of ROS in hAMSC after BPA exposure, trigger the downstream activation of several signalling pathways. ROS accumulation activates an antioxidant response, which is marked by an elevated production of Nrf2 and HO-1. Concurrently, the high ROS level induce sterile inflammation, resulting in increased transcription of factors involved in inflammasome complex formation and activation. However, this increased transcription does not translate to higher production of IL-1β, the downstream effector of the inflammasome pathway. Instead, oxidative stress promotes p53 stabilization and upregulates p21 and p27 genes, as well as components of the senescence-associated secretory phenotype (SASP). Ultimately, the senescent state serves as a prelude to apoptosis, which occurs when hAMSC are exposed to the highest BPA concentrations.

Article Snippet: Subsequently, cells were washed three times with Tris Buffered Saline (TBS) for 5 min each. hAMSC were incubated with p21 primary antibody (Waf1/Cip1/CDKN1A p21, sc-6246, Santa Cruz Biotechnology, Texas, USA), diluted 1:100 in normal goat serum (NGS, Invitrogen, Waltham USA, #10000C) and incubated overnight at 4 °C in the dark.

Techniques: Activation Assay, Sterility