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cd86  (Miltenyi Biotec)


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    Miltenyi Biotec cd86
    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01
    Cd86, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd86/CD86+Antibody%2C+anti-mouse%2C+REAfinity/pmc13694607-279-27-31
    Average 94 stars, based on 21 article reviews
    cd86 - by Bioz Stars, 2026-11
    94/100 stars

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    1) Product Images from "EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis"

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    Journal: Molecular Biomedicine

    doi: 10.1186/s43556-026-00643-w

    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01
    Figure Legend Snippet: EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Techniques Used: Inhibition, Derivative Assay, Isolation, Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Software

    Related Articles

    Staining:

    Article Title: Bifidobacteria support optimal infant vaccine responses
    Article Snippet: For assessment of CD86, CD80 and MCHII expression, single-cell suspensions of spleen or lymph nodes were stained with Zombie Aqua Fixable Viability Stain 780 (1:1000, BioLegend) and murine Fc-blocking antibody clone 2.4G2 (1:100, BD Biosciences) in PBS for 15 min. .. Cells were washed and then stained with fluorochrome-conjugated antibodies in FACS buffer (PBS, 0.1% bovine serum albumin, 2 mM EDTA) against CD19 (ID3, 1:200, BD), CD3 (145-2C11, 1:150, BD Biosciences), CD8 (53-6.7, 1:200, BD), NK1.1 (PK136, 1:200, BD), Ly6G (1A8, 1:600, BD), B220 (RA3-6B2, 1:3000, BD), CD11b (M1/70, 1:500, BD), CD11c (HL3, 1:400, BD Biosciences), MHCII (M5/114.15.2, 1:150, Miltenyi), CD86 (PO3.3, 1:150, Miltenyi) and CD80 (16-10A1, 1:150, Tonbo Biosciences). .. Mediastinal lymph nodes were fixed in 1 ml 4% paraformaldehyde (Sigma) for 4 h and then incubated in 1 ml 30% sucrose solution overnight at 4 °C before being embedded in OCT (Tissue Tek) as described previously .

    Article Title: Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages
    Article Snippet: After THP-1 differentiation into macrophages, or their further polarisation into M1- and M2-like macrophages, cells were washed twice with PBS without Ca 2+ and Mg 2+ (Gibco) and detached with Versene (Gibco). .. Macrophage surface markers expression was assessed via flow cytometry by staining the cells with anti CD11b (FITC, #130-110-552, Miltenyi Biotec), anti CD14 (APC, #130-110-520, Miltenyi Biotec), anti CD36 (APC-Vio770, #130-110-743, Miltenyi Biotec), anti CD86 (PerCP-Vio700, #130-116-267, Miltenyi Biotec), anti HLA-DR (VioGreen, #130-111-795, Miltenyi Biotec), anti CD163 (PE, #130-112-128, Miltenyi Biotec), anti CD206 (VioBlue, #130-127-809, Miltenyi Biotec) according to manufacturer’s instructions. ..

    FACS:

    Article Title: Bifidobacteria support optimal infant vaccine responses
    Article Snippet: For assessment of CD86, CD80 and MCHII expression, single-cell suspensions of spleen or lymph nodes were stained with Zombie Aqua Fixable Viability Stain 780 (1:1000, BioLegend) and murine Fc-blocking antibody clone 2.4G2 (1:100, BD Biosciences) in PBS for 15 min. .. Cells were washed and then stained with fluorochrome-conjugated antibodies in FACS buffer (PBS, 0.1% bovine serum albumin, 2 mM EDTA) against CD19 (ID3, 1:200, BD), CD3 (145-2C11, 1:150, BD Biosciences), CD8 (53-6.7, 1:200, BD), NK1.1 (PK136, 1:200, BD), Ly6G (1A8, 1:600, BD), B220 (RA3-6B2, 1:3000, BD), CD11b (M1/70, 1:500, BD), CD11c (HL3, 1:400, BD Biosciences), MHCII (M5/114.15.2, 1:150, Miltenyi), CD86 (PO3.3, 1:150, Miltenyi) and CD80 (16-10A1, 1:150, Tonbo Biosciences). .. Mediastinal lymph nodes were fixed in 1 ml 4% paraformaldehyde (Sigma) for 4 h and then incubated in 1 ml 30% sucrose solution overnight at 4 °C before being embedded in OCT (Tissue Tek) as described previously .

    Immunopeptidomics:

    Article Title: Melanoma immunotherapy by nanosphere-vaccine elicited CD4+ and CD8+ T-cell response for tumor regression.
    Article Snippet: Word count: 150 Manuscript Word Count: 12305 Number of References= 77 Number of Figures=14 (Main Manuscript) and 9 (Supplementary File) Jo ur na l P re -p ro of Journal Pre-proof 2 Conflicts of interest None.. Funding KRB is funded in part by a Natural Science & Engineering Research program discovery grant.. NP is funded in part by an Indian Council of Medical Research grant (DDR-IIRP23-1583) Abstract Melanoma, driven by defective immune surveillance and cancer-cell evasion, has rising morbidity and mortality due to solar radiation exposure and delayed diagnosis.

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis
    Article Snippet: For intracellular staining, cells were fixed and permeabilized using the Inside Stain kit according to the manufacturer’s instructions (Miltenyi Biotec) before intracellular antibody staining. .. The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec). ..

    Bioprocessing:

    Article Title: Neuroinflammatory Signaling and Immune Cell Infiltration Differ in Brains of Rats Exposed to Space Radiation and Social Isolation
    Article Snippet: .. The following monoclonal antibodies were used at a 1:100 dilution factor unless otherwise indicated for spectral flow cytometric analysis: CD4 (Miltenyi, clone REA482, cat. #130-123-286), CD8a (Miltenyi, clone REA437, cat. #130-108-878), CD3 (Miltenyi, clone REA223, cat. #130-127-365, 1:400), CD11b/c (Miltenyi, clone REA325, cat. #130-105-273), CD45RA (Biolegend, San Diego, CA, USA, clone OX-33, cat. #202318), MHCII (Miltenyi, clone REA564, cat. #130-108-711), CD25 (Biolegend, clone OX-39, cat. #202105, 1:200 dilution), CD45RC (Miltenyi, clone REA1000, cat. #130-116-903), CD134 (Miltenyi, clone REA540, cat. #130-108-188, 1:50 dilution), CD71 (Biolegend, clone OX-26, cat. #204412), CD43 (Biolegend, clone W313, cat. #202810), CD45 (Biolegend, clone OX-1, cat. #202218, 1:200 dilution), and CD86 (Miltenyi, clone 24F, cat. #130-109-132, 1:200 dilution). .. Data were acquired on a Cytek ® Aurora 4L 16V-14B-10YG-8R spectral flow cytometer and analyzed with Cytek SpectroFlo ® (Cytek Biosciences; Fremont, CA, USA).

    Article Title: Neuroinflammatory Signaling and Immune Cell Infiltration Differ in Brains of Rats Exposed to Space Radiation and Social Isolation.
    Article Snippet: .. The following monoclonal antibodies were used at a 1:100 dilution factor unless otherwise indicated for spectral flow cytometric analysis: CD4 (Miltenyi, clone REA482, cat. #130-123-286), CD8a (Miltenyi, clone REA437, cat. #130-108-878), CD3 (Miltenyi, clone REA223, cat. #130-127-365, 1:400), CD11b/c (Miltenyi, clone REA325, cat. #130-105-273), CD45RA (Biolegend, San Diego, CA, USA, clone OX-33, cat. #202318), MHCII (Miltenyi, clone REA564, cat. #130-108-711), CD25 (Biolegend, clone OX-39, cat. #202105, 1:200 dilution), CD45RC (Miltenyi, clone REA1000, cat. #130-116-903), CD134 (Miltenyi, clone REA540, cat. #130-108-188, 1:50 dilution), CD71 (Biolegend, clone OX-26, cat. #204412), CD43 (Biolegend, clone W313, cat. #202810), CD45 (Biolegend, clone OX-1, cat. #202218, 1:200 dilution), and CD86 (Miltenyi, clone 24F, cat. #130-109-132, 1:200 dilution). .. Data were acquired on a Cytek® Aurora 4L 16V-14B-10YG-8R spectral flow cytometer and analyzed with Cytek SpectroFlo® (Cytek Biosciences; Fremont, CA, USA).

    other:

    Article Title: Effects of Repeated Early-life Anesthesia Exposure on Visual System Development in Mice
    Article Snippet: CD86 , Miltenyi Biotec , 130-122-129 , , 1:20 , REA1190 , PE.

    Immune Cell:

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis
    Article Snippet: For intracellular staining, cells were fixed and permeabilized using the Inside Stain kit according to the manufacturer’s instructions (Miltenyi Biotec) before intracellular antibody staining. .. The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec). ..

    Expressing:

    Article Title: Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages
    Article Snippet: After THP-1 differentiation into macrophages, or their further polarisation into M1- and M2-like macrophages, cells were washed twice with PBS without Ca 2+ and Mg 2+ (Gibco) and detached with Versene (Gibco). .. Macrophage surface markers expression was assessed via flow cytometry by staining the cells with anti CD11b (FITC, #130-110-552, Miltenyi Biotec), anti CD14 (APC, #130-110-520, Miltenyi Biotec), anti CD36 (APC-Vio770, #130-110-743, Miltenyi Biotec), anti CD86 (PerCP-Vio700, #130-116-267, Miltenyi Biotec), anti HLA-DR (VioGreen, #130-111-795, Miltenyi Biotec), anti CD163 (PE, #130-112-128, Miltenyi Biotec), anti CD206 (VioBlue, #130-127-809, Miltenyi Biotec) according to manufacturer’s instructions. ..

    Flow Cytometry:

    Article Title: Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages
    Article Snippet: After THP-1 differentiation into macrophages, or their further polarisation into M1- and M2-like macrophages, cells were washed twice with PBS without Ca 2+ and Mg 2+ (Gibco) and detached with Versene (Gibco). .. Macrophage surface markers expression was assessed via flow cytometry by staining the cells with anti CD11b (FITC, #130-110-552, Miltenyi Biotec), anti CD14 (APC, #130-110-520, Miltenyi Biotec), anti CD36 (APC-Vio770, #130-110-743, Miltenyi Biotec), anti CD86 (PerCP-Vio700, #130-116-267, Miltenyi Biotec), anti HLA-DR (VioGreen, #130-111-795, Miltenyi Biotec), anti CD163 (PE, #130-112-128, Miltenyi Biotec), anti CD206 (VioBlue, #130-127-809, Miltenyi Biotec) according to manufacturer’s instructions. ..



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    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01
    Cd86 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd86  (Boster Bio)
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    Boster Bio cd86
    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01
    Cd86, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd86/Anti-CD86+Antibody/pm42874731-102-24-25
    Average 94 stars, based on 1 article reviews
    cd86 - by Bioz Stars, 2026-11
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    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Journal: Molecular Biomedicine

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    doi: 10.1186/s43556-026-00643-w

    Figure Lengend Snippet: EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Article Snippet: The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec).

    Techniques: Inhibition, Derivative Assay, Isolation, Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Software