Review



dead cell removal kit  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Miltenyi Biotec dead cell removal kit
    Dead Cell Removal Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 2503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8/Dead+Cell+Removal+Kit/custom%40130-090-101%4042466067
    Average 99 stars, based on 2503 article reviews
    dead cell removal kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Activation Assay:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Expressing:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Isolation:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Article Title: Tumor Cell Death Drives Tumor-Promoting IL-6⁺ iCAF formation via P2X7-activation
    Article Snippet: Results were plotted against a standard curve generated from measurements of 0.001-1 μM ATP (Sigma-Aldrich, A6419-1G) using Microsoft Excel. .. CD3+ T cells were isolated from splenocyte of OT-I transgenic mouse (003831 - OT-1 Strain Details ( jax.org )) using the CD8-MACS (Miltenyi Biotec, 130-117-044) according to the manufacturer’s protocol. .. Target tumor cells (MC38-OVA cell line) were seeded 20 hours before the start of the experiment on fibronectin-coated 96-well imaging plates (Screenstar Microplate, Greiner Bio-One 655866; fibronectin: R&D Systems 1030-FN-05M).

    Article Title: Targeting stroma-mediated T-cell exclusion and functional exhaustion in pancreatic ductal adenocarcinoma through CXCR4 and PD-1 blockade
    Article Snippet: .. T-cells were subsequently isolated from PBMC using MagneticActivated Cell Sorting (MACS) with biotinylated CD4 (#130-113- 224, Miltenyi Biotec, Bergisch Gladbach, Germany) and CD8 (#130-110-676, Miltenyi Biotec) antibodies, followed by antibiotin microbeads (#130-090-485, Miltenyi Biotec) according to the manufacturer’s instructions, employing the MidiMACS separator (#130-042-302, Miltenyi Biotec). .. The purified T-cells were maintained in RPMI medium (#21875-034, Gibco) supplemented with 10% FBS, 1% penicillin–streptomycin, and 50 ng/mL IL-2 (#11340025, ImmunoTools, Friesoythe, Germany).

    Staining:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Article Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
    Article Snippet: .. After washing, the cells were resuspended in Dulbecco’s phosphate-buffered saline (HyClone, Logan, UT, USA) and stained with fluorochrome-conjugated monoclonal antibodies against human CD3, CD4, and CD8 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). .. The stained cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

    Immunopeptidomics:

    Article Title: A targetable dependency on nonsense-mediated decay for proteostasis and immune control in small cell lung cancer
    Article Snippet: Flow cytometry data was analyzed with Flowjo software. .. Human primary antibodies used were B2M (Molecular probes #A15770), MHC-ABC (Invitrogen #11998342), CD45 (Miltenyi #130110636), CD3 (Miltenyi #130113142), CD4 (Biolegend #357410), CD8 (Miltenyi #130110678), CD56 (Miltenyi #130113310), CD11b (Miltenyi #130110556). .. Murine primary antibodies used were CD45 (Miltenyi #130110665), CD3 (Miltenyi #130119793), Nkp46 (BD Biosciences #3122669), CD19 (Miltenyi #130112037), CD11b (Miltenyi #1301138063), CD14 (Miltenyi #130115559), H2-Kb (Miltenyi #130115586), IFN-γ (ThermoFisher #48731182), KI-67 (Miltenyi #130120418).

    Transgenic Assay:

    Article Title: Tumor Cell Death Drives Tumor-Promoting IL-6⁺ iCAF formation via P2X7-activation
    Article Snippet: Results were plotted against a standard curve generated from measurements of 0.001-1 μM ATP (Sigma-Aldrich, A6419-1G) using Microsoft Excel. .. CD3+ T cells were isolated from splenocyte of OT-I transgenic mouse (003831 - OT-1 Strain Details ( jax.org )) using the CD8-MACS (Miltenyi Biotec, 130-117-044) according to the manufacturer’s protocol. .. Target tumor cells (MC38-OVA cell line) were seeded 20 hours before the start of the experiment on fibronectin-coated 96-well imaging plates (Screenstar Microplate, Greiner Bio-One 655866; fibronectin: R&D Systems 1030-FN-05M).

    FACS:

    Article Title: Targeting stroma-mediated T-cell exclusion and functional exhaustion in pancreatic ductal adenocarcinoma through CXCR4 and PD-1 blockade
    Article Snippet: .. T-cells were subsequently isolated from PBMC using MagneticActivated Cell Sorting (MACS) with biotinylated CD4 (#130-113- 224, Miltenyi Biotec, Bergisch Gladbach, Germany) and CD8 (#130-110-676, Miltenyi Biotec) antibodies, followed by antibiotin microbeads (#130-090-485, Miltenyi Biotec) according to the manufacturer’s instructions, employing the MidiMACS separator (#130-042-302, Miltenyi Biotec). .. The purified T-cells were maintained in RPMI medium (#21875-034, Gibco) supplemented with 10% FBS, 1% penicillin–streptomycin, and 50 ng/mL IL-2 (#11340025, ImmunoTools, Friesoythe, Germany).

    Magnetic Cell Separation:

    Article Title: Targeting stroma-mediated T-cell exclusion and functional exhaustion in pancreatic ductal adenocarcinoma through CXCR4 and PD-1 blockade
    Article Snippet: .. T-cells were subsequently isolated from PBMC using MagneticActivated Cell Sorting (MACS) with biotinylated CD4 (#130-113- 224, Miltenyi Biotec, Bergisch Gladbach, Germany) and CD8 (#130-110-676, Miltenyi Biotec) antibodies, followed by antibiotin microbeads (#130-090-485, Miltenyi Biotec) according to the manufacturer’s instructions, employing the MidiMACS separator (#130-042-302, Miltenyi Biotec). .. The purified T-cells were maintained in RPMI medium (#21875-034, Gibco) supplemented with 10% FBS, 1% penicillin–streptomycin, and 50 ng/mL IL-2 (#11340025, ImmunoTools, Friesoythe, Germany).

    Saline:

    Article Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
    Article Snippet: .. After washing, the cells were resuspended in Dulbecco’s phosphate-buffered saline (HyClone, Logan, UT, USA) and stained with fluorochrome-conjugated monoclonal antibodies against human CD3, CD4, and CD8 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). .. The stained cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

    Bioprocessing:

    Article Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
    Article Snippet: .. After washing, the cells were resuspended in Dulbecco’s phosphate-buffered saline (HyClone, Logan, UT, USA) and stained with fluorochrome-conjugated monoclonal antibodies against human CD3, CD4, and CD8 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). .. The stained cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

    Cell Isolation:

    Article Title: Selective blockade of γc pathway ameliorates alopecia areata in a humanized murine model.
    Article Snippet: Alopecia areata (AA) is a chronic autoimmune disease characterized by sudden patchy hair loss and persistent inflammation.. To date, only JAK inhibitors have been approved for AA treatment, but FDA-issued black box warnings highlight the need for alternative therapies.. We previously reported that an anti-γc antibody, hC2, inhibits autoreactive B, T, and NK cells by selectively attenuating JAK/STAT signaling induced by six γc cytokines, without affecting off-target TEC kinase pathways.

    Injection:

    Article Title: Selective blockade of γc pathway ameliorates alopecia areata in a humanized murine model.
    Article Snippet: Alopecia areata (AA) is a chronic autoimmune disease characterized by sudden patchy hair loss and persistent inflammation.. To date, only JAK inhibitors have been approved for AA treatment, but FDA-issued black box warnings highlight the need for alternative therapies.. We previously reported that an anti-γc antibody, hC2, inhibits autoreactive B, T, and NK cells by selectively attenuating JAK/STAT signaling induced by six γc cytokines, without affecting off-target TEC kinase pathways.



    Similar Products

    99
    NSJ Bioreagents cd8 antibody
    Cd8 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8/CD8+Antibody/custom%40v2383%4042682916
    Average 99 stars, based on 1 article reviews
    cd8 antibody - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    94
    MedChemExpress immunomagnetic separation kit
    Immunomagnetic Separation Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8/Human+CD8%2B+T+Cells+Negative+Selection+Kit/pmc13458028-145-14-25
    Average 94 stars, based on 1 article reviews
    immunomagnetic separation kit - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    99
    Miltenyi Biotec dead cell removal kit
    Dead Cell Removal Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8/Dead+Cell+Removal+Kit/custom%40130-090-101%4042466067
    Average 99 stars, based on 1 article reviews
    dead cell removal kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results