cd8 Search Results


naïve cd8 t cells  (ATCC)
96
ATCC naïve cd8 t cells
Naïve Cd8 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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naı̈ve cd8 t cell isolation kit  (Miltenyi Biotec)
99
Miltenyi Biotec naı̈ve cd8 t cell isolation kit
Naı̈ve Cd8 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human cd8  (Bio-Rad)
94
Bio-Rad anti human cd8
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Anti Human Cd8, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8  (Miltenyi Biotec)
95
Miltenyi Biotec cd8
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Cd8, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd8/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
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cd8 microbead kit  (Miltenyi Biotec)
94
Miltenyi Biotec cd8 microbead kit
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Cd8 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd8 microbead kit/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
cd8 microbead kit - by Bioz Stars, 2026-03
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antibody coated microbeads  (Miltenyi Biotec)
94
Miltenyi Biotec antibody coated microbeads
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Antibody Coated Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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naive cd8 t cell isolation kit  (Miltenyi Biotec)
95
Miltenyi Biotec naive cd8 t cell isolation kit
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Naive Cd8 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd8 pe  (Bio-Rad)
95
Bio-Rad anti cd8 pe
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Anti Cd8 Pe, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd8a  (Miltenyi Biotec)
96
Miltenyi Biotec anti cd8a
FIGURE 1. MBP-stimulated CD4 and <t>CD8</t> expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.
Anti Cd8a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd8 apc cy7 mabs  (R&D Systems)
95
R&D Systems anti cd8 apc cy7 mabs
Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of <t>CD3+CD8+</t> T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).
Anti Cd8 Apc Cy7 Mabs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8 microbeads  (Miltenyi Biotec)
99
Miltenyi Biotec cd8 microbeads
Freshly isolated PBMCs were stimulated with plate-bound anti-CD3/CD28 for 3 days. T-cells proliferation was measured by CFSE dilution, and apoptosis was measured by Annexin V staining. (A), Proliferation rates of total CD4 + and <t>CD8</t> + T-cells from the CHC and HD groups. (B), Representative FACS profiles of T-cells proliferation for each group of CHC patients and HDs. (C), Apoptosis rates of CD4 + and CD8 + T-cells from the CHC patients and HDs with or without anti-CD3/CD28 stimulation. (D), Representative FACS profiles of T-cells apoptosis for each group of CHC patients and HDs. For (A) and (C), each dot represents one individual. *, p<0.05.
Cd8 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc conjugated rat anti human cd8 antibody  (Bio-Rad)
93
Bio-Rad fitc conjugated rat anti human cd8 antibody
Freshly isolated PBMCs were stimulated with plate-bound anti-CD3/CD28 for 3 days. T-cells proliferation was measured by CFSE dilution, and apoptosis was measured by Annexin V staining. (A), Proliferation rates of total CD4 + and <t>CD8</t> + T-cells from the CHC and HD groups. (B), Representative FACS profiles of T-cells proliferation for each group of CHC patients and HDs. (C), Apoptosis rates of CD4 + and CD8 + T-cells from the CHC patients and HDs with or without anti-CD3/CD28 stimulation. (D), Representative FACS profiles of T-cells apoptosis for each group of CHC patients and HDs. For (A) and (C), each dot represents one individual. *, p<0.05.
Fitc Conjugated Rat Anti Human Cd8 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 1. MBP-stimulated CD4 and CD8 expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 1. MBP-stimulated CD4 and CD8 expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

FIGURE 4. Annexin V- and PD-1-expressing MBP-stimulated CD4 and CD8 T lymphocytes. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, MBP-stimulated CD4 T lymphocytes expressing annexin V and PD-1. Bottom panels, MBP-stimulated CD8 T lymphocytes expressing annexin V and PD-1. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 4. Annexin V- and PD-1-expressing MBP-stimulated CD4 and CD8 T lymphocytes. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, MBP-stimulated CD4 T lymphocytes expressing annexin V and PD-1. Bottom panels, MBP-stimulated CD8 T lymphocytes expressing annexin V and PD-1. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

FIGURE 5. pAkt-expressing MBP-stimulated CD4 and CD8 T lymphocytes. Representative results of MBP-stimulated PBMC of patients with either AMS or stable SMS are shown. Top panels, MBP-stimulated CD4 T lymphocytes expressing pAkt. Bottom panels, MBP-stimulated CD8 T lymphocytes expressing pAkt. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 5. pAkt-expressing MBP-stimulated CD4 and CD8 T lymphocytes. Representative results of MBP-stimulated PBMC of patients with either AMS or stable SMS are shown. Top panels, MBP-stimulated CD4 T lymphocytes expressing pAkt. Bottom panels, MBP-stimulated CD8 T lymphocytes expressing pAkt. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

FIGURE 6. Blockade of the PD-1-PD-L1 pathway. pAKT-expressing, MBP-stimulated CD4 and CD8 T lymphocytes.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 6. Blockade of the PD-1-PD-L1 pathway. pAKT-expressing, MBP-stimulated CD4 and CD8 T lymphocytes.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of CD3+CD8+ T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).

Journal: Science China. Life sciences

Article Title: Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model.

doi: 10.1007/s11427-012-4370-3

Figure Lengend Snippet: Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of CD3+CD8+ T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).

Article Snippet: A 50 μL sample of the whole blood obtained after stimulation was incubated with 10 μL of anti-CD3-PerCp and 5 μL of anti-CD8-APC-Cy7 mAbs in the dark at 4°C for 30 min. After haematolysis and washing with PBS, the resuspended cells were incubated in the dark at 4°C for 45 min with 10 μL anti-rat GITR-PE mAb (R&D Systems, Minneapolis, MN, USA) or 10 μL IgG1-PE (Beckman Coulter Inc) as an isotype-control.

Techniques: Activity Assay, Expressing, Control

Freshly isolated PBMCs were stimulated with plate-bound anti-CD3/CD28 for 3 days. T-cells proliferation was measured by CFSE dilution, and apoptosis was measured by Annexin V staining. (A), Proliferation rates of total CD4 + and CD8 + T-cells from the CHC and HD groups. (B), Representative FACS profiles of T-cells proliferation for each group of CHC patients and HDs. (C), Apoptosis rates of CD4 + and CD8 + T-cells from the CHC patients and HDs with or without anti-CD3/CD28 stimulation. (D), Representative FACS profiles of T-cells apoptosis for each group of CHC patients and HDs. For (A) and (C), each dot represents one individual. *, p<0.05.

Journal: PLoS ONE

Article Title: T Lymphocytes from Chronic HCV-Infected Patients Are Primed for Activation-Induced Apoptosis and Express Unique Pro-Apoptotic Gene Signature

doi: 10.1371/journal.pone.0077008

Figure Lengend Snippet: Freshly isolated PBMCs were stimulated with plate-bound anti-CD3/CD28 for 3 days. T-cells proliferation was measured by CFSE dilution, and apoptosis was measured by Annexin V staining. (A), Proliferation rates of total CD4 + and CD8 + T-cells from the CHC and HD groups. (B), Representative FACS profiles of T-cells proliferation for each group of CHC patients and HDs. (C), Apoptosis rates of CD4 + and CD8 + T-cells from the CHC patients and HDs with or without anti-CD3/CD28 stimulation. (D), Representative FACS profiles of T-cells apoptosis for each group of CHC patients and HDs. For (A) and (C), each dot represents one individual. *, p<0.05.

Article Snippet: CD4 + and CD8 + T-cells were purified from PBMCs using CD4 and CD8 microbeads with an autoMACS Separator (Miltenyi Biotec, CA, USA) following the manufacturer’s instructions.

Techniques: Isolation, Staining

(A), Cytokine levels, including IFN-γ, IL-1β, IL-9, and IP-10, in the plasma of CHC patients and HDs as measured by Luminex assay. (B), Effect of pre-incubation of PBMCs with the indicated cytokines on T-cells proliferation as measured by CFSE dilution and T-cells apoptosis as measured by Annexin-V staining. PBMCs from HDs were pre-incubated with or without (NC) the indicated cytokines for 48 hrs and then stimulated with anti-CD3/CD28 for 3 days before the measurement of proliferation and apoptosis. Data were from 5 independent HDs. (C), Representative FACS profiles of CD4 + and CD8 + T-cells apoptosis after pre-incubation with cytokines followed by stimulation. *, p<0.05; **, p<0.01.

Journal: PLoS ONE

Article Title: T Lymphocytes from Chronic HCV-Infected Patients Are Primed for Activation-Induced Apoptosis and Express Unique Pro-Apoptotic Gene Signature

doi: 10.1371/journal.pone.0077008

Figure Lengend Snippet: (A), Cytokine levels, including IFN-γ, IL-1β, IL-9, and IP-10, in the plasma of CHC patients and HDs as measured by Luminex assay. (B), Effect of pre-incubation of PBMCs with the indicated cytokines on T-cells proliferation as measured by CFSE dilution and T-cells apoptosis as measured by Annexin-V staining. PBMCs from HDs were pre-incubated with or without (NC) the indicated cytokines for 48 hrs and then stimulated with anti-CD3/CD28 for 3 days before the measurement of proliferation and apoptosis. Data were from 5 independent HDs. (C), Representative FACS profiles of CD4 + and CD8 + T-cells apoptosis after pre-incubation with cytokines followed by stimulation. *, p<0.05; **, p<0.01.

Article Snippet: CD4 + and CD8 + T-cells were purified from PBMCs using CD4 and CD8 microbeads with an autoMACS Separator (Miltenyi Biotec, CA, USA) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Luminex, Incubation, Staining