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cd63 gfp vector  (Addgene inc)


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    Addgene inc cd63 gfp vector
    Cd63 Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd63+gfp+vector/CD63-pEGFP+C2+(Plasmid+%2362964)/pmc11934211-50-19-22
    Average 95 stars, based on 93 article reviews
    cd63 gfp vector - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Capillary Electrochromatography:

    Article Title: Enhanced Small Extracellular Vesicle Uptake by Activated Interneurons Improves Stroke Recovery in Mice
    Article Snippet: .. To track the brain distribution of exogenous CEC‐sEVs, CEC‐sEVs with CD63‐GFP (GFP‐sEVs) were generated by transfecting CECs with a CD63‐GFP vector (CD63‐pEGFP, Addgene #62964) and characterized according to our published protocols (Zhang et al. , ). ..

    Generated:

    Article Title: Enhanced Small Extracellular Vesicle Uptake by Activated Interneurons Improves Stroke Recovery in Mice
    Article Snippet: .. To track the brain distribution of exogenous CEC‐sEVs, CEC‐sEVs with CD63‐GFP (GFP‐sEVs) were generated by transfecting CECs with a CD63‐GFP vector (CD63‐pEGFP, Addgene #62964) and characterized according to our published protocols (Zhang et al. , ). ..



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    Exo-miR-15b-3p high expression in GC patient serum as a GC diagnosis and prognosis biomarker. a Representative electron microscopy micrographs of SGC-7901, BGC-823 and GES-1 cell conditioning medium secreted exosomes, as well as control and GC patient serum secreted exosomes. Scale bar, 100 nm. b Nano Sight particle-tracking analysis to determine exosome size distribution and number. c Levels of exosomal markers, TSG101, <t>CD63</t> and CD9, of cells and serum derived exosomes determined using western blotting analysis. d Relative expressions of Exo-miR-15b-3p in conditioned medium of BGC-823, SGC-7901 and GES-1 cells. e Relative exosomal miR-15b-3p levels in GC patients and non-GC normal volunteer ( n = 108) serum. f The sensitivity and specificity of serum Exo-miR-15b-3p for GC prediction was evaluated through Receiver-operating characteristic (ROC) curve analysis. g The correlation between serum Exo-miR-15b-3p expression and overall survival of the 108 GC patients determined using Kaplan–Meier analysis. The cutoff used was the median. Mean ± SEM of the results are presented
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    Exo-miR-15b-3p high expression in GC patient serum as a GC diagnosis and prognosis biomarker. a Representative electron microscopy micrographs of SGC-7901, BGC-823 and GES-1 cell conditioning medium secreted exosomes, as well as control and GC patient serum secreted exosomes. Scale bar, 100 nm. b Nano Sight particle-tracking analysis to determine exosome size distribution and number. c Levels of exosomal markers, TSG101, <t>CD63</t> and CD9, of cells and serum derived exosomes determined using western blotting analysis. d Relative expressions of Exo-miR-15b-3p in conditioned medium of BGC-823, SGC-7901 and GES-1 cells. e Relative exosomal miR-15b-3p levels in GC patients and non-GC normal volunteer ( n = 108) serum. f The sensitivity and specificity of serum Exo-miR-15b-3p for GC prediction was evaluated through Receiver-operating characteristic (ROC) curve analysis. g The correlation between serum Exo-miR-15b-3p expression and overall survival of the 108 GC patients determined using Kaplan–Meier analysis. The cutoff used was the median. Mean ± SEM of the results are presented
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    Exo-miR-15b-3p high expression in GC patient serum as a GC diagnosis and prognosis biomarker. a Representative electron microscopy micrographs of SGC-7901, BGC-823 and GES-1 cell conditioning medium secreted exosomes, as well as control and GC patient serum secreted exosomes. Scale bar, 100 nm. b Nano Sight particle-tracking analysis to determine exosome size distribution and number. c Levels of exosomal markers, TSG101, CD63 and CD9, of cells and serum derived exosomes determined using western blotting analysis. d Relative expressions of Exo-miR-15b-3p in conditioned medium of BGC-823, SGC-7901 and GES-1 cells. e Relative exosomal miR-15b-3p levels in GC patients and non-GC normal volunteer ( n = 108) serum. f The sensitivity and specificity of serum Exo-miR-15b-3p for GC prediction was evaluated through Receiver-operating characteristic (ROC) curve analysis. g The correlation between serum Exo-miR-15b-3p expression and overall survival of the 108 GC patients determined using Kaplan–Meier analysis. The cutoff used was the median. Mean ± SEM of the results are presented

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Exosomal transfer of miR-15b-3p enhances tumorigenesis and malignant transformation through the DYNLT1/Caspase-3/Caspase-9 signaling pathway in gastric cancer

    doi: 10.1186/s13046-019-1511-6

    Figure Lengend Snippet: Exo-miR-15b-3p high expression in GC patient serum as a GC diagnosis and prognosis biomarker. a Representative electron microscopy micrographs of SGC-7901, BGC-823 and GES-1 cell conditioning medium secreted exosomes, as well as control and GC patient serum secreted exosomes. Scale bar, 100 nm. b Nano Sight particle-tracking analysis to determine exosome size distribution and number. c Levels of exosomal markers, TSG101, CD63 and CD9, of cells and serum derived exosomes determined using western blotting analysis. d Relative expressions of Exo-miR-15b-3p in conditioned medium of BGC-823, SGC-7901 and GES-1 cells. e Relative exosomal miR-15b-3p levels in GC patients and non-GC normal volunteer ( n = 108) serum. f The sensitivity and specificity of serum Exo-miR-15b-3p for GC prediction was evaluated through Receiver-operating characteristic (ROC) curve analysis. g The correlation between serum Exo-miR-15b-3p expression and overall survival of the 108 GC patients determined using Kaplan–Meier analysis. The cutoff used was the median. Mean ± SEM of the results are presented

    Article Snippet: Genechem Inc. (China) constructed luciferase-labelled lentivirus vectors carrying miR-15b-3p (Lv-miR-15b-3p)/negative control (Lv-NC), miR-15b-3p inhibitor (Lv-inhibitor)/negative control (Lv-inNC) and GFP-labelled lentivirus vectors containing CD63 (GFP-Lv-CD63) were used.

    Techniques: Expressing, Biomarker Discovery, Electron Microscopy, Control, Derivative Assay, Western Blot

    Exosome-mediated miRNA transport between cells. a Internalization of PKH26-labelled exosomes (red) in GES-1 and SGC-7901 cells were observed through confocal microscopy. Fluorescein phalloidin-FITC (green) was used to stain F-actin, while DAPI (blue) was used to stain nuclei. Scale bar, 20 μm. b Exosomes (green) isolated from BGC-823 cell conditioning medium labeled with GFP-Lv-CD63 (green) and transfected with Cy3-miR-15b-3p (red) were co-cultured with SGC-7901 and GES-1 cells for 24 h, and the fluorescent signals were detected using confocal microscopy. Nuclei are stained blue (DAPI). Scale bar, 20 μm. c The efficiency of exosomes in delivering miR-15b-3p to GES-1 and SGC-7901 cells was analyzed using RT-PCR. Mean ± SEM of the results are presented

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Exosomal transfer of miR-15b-3p enhances tumorigenesis and malignant transformation through the DYNLT1/Caspase-3/Caspase-9 signaling pathway in gastric cancer

    doi: 10.1186/s13046-019-1511-6

    Figure Lengend Snippet: Exosome-mediated miRNA transport between cells. a Internalization of PKH26-labelled exosomes (red) in GES-1 and SGC-7901 cells were observed through confocal microscopy. Fluorescein phalloidin-FITC (green) was used to stain F-actin, while DAPI (blue) was used to stain nuclei. Scale bar, 20 μm. b Exosomes (green) isolated from BGC-823 cell conditioning medium labeled with GFP-Lv-CD63 (green) and transfected with Cy3-miR-15b-3p (red) were co-cultured with SGC-7901 and GES-1 cells for 24 h, and the fluorescent signals were detected using confocal microscopy. Nuclei are stained blue (DAPI). Scale bar, 20 μm. c The efficiency of exosomes in delivering miR-15b-3p to GES-1 and SGC-7901 cells was analyzed using RT-PCR. Mean ± SEM of the results are presented

    Article Snippet: Genechem Inc. (China) constructed luciferase-labelled lentivirus vectors carrying miR-15b-3p (Lv-miR-15b-3p)/negative control (Lv-NC), miR-15b-3p inhibitor (Lv-inhibitor)/negative control (Lv-inNC) and GFP-labelled lentivirus vectors containing CD63 (GFP-Lv-CD63) were used.

    Techniques: Confocal Microscopy, Staining, Isolation, Labeling, Transfection, Cell Culture, Reverse Transcription Polymerase Chain Reaction