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anti dog cd5 pacific bluetm ykix322 3 bio rad mca1037pb mouse anti dog cd94 alexa fluor 647 (Bio-Rad)

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Bio-Rad anti dog cd5 pacific bluetm ykix322 3 bio rad mca1037pb mouse anti dog cd94 alexa fluor 647
Anti Dog Cd5 Pacific Bluetm Ykix322 3 Bio Rad Mca1037pb Mouse Anti Dog Cd94 Alexa Fluor 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd5+bio+rad/Mouse+anti+Dog+CD18%3AAlexa+Fluor+647/pm39462364-99-25-29
Average 93 stars, based on 8 article reviews

anti dog cd5 pacific bluetm ykix322 3 bio rad mca1037pb mouse anti dog cd94 alexa fluor 647 - by Bioz Stars, 2026-09

93/100 stars

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Diagnostic Assay:

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs
Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Antibody Labeling:

Labeling:

Marker:

Isolation:

Modification:

Staining:

Cell Counting:

Fluorescence:

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Conjugated antibodies used for triple-labeling cells in acute leukemia immunophenotyping panels after April 2021, including their target antigen and registry number (when available) or source.

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Conjugated antibodies used for triple-labeling cells in acute leukemia immunophenotyping panels after April 2021, including their target antigen and registry number (when available) or source.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques:

Conjugated antibodies used for triple-labeling cells in a lymphoma immunophenotyping panel used for routine diagnostic testing after April 2021, including their target antigen*.

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Conjugated antibodies used for triple-labeling cells in a lymphoma immunophenotyping panel used for routine diagnostic testing after April 2021, including their target antigen*.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Diagnostic Assay

Flow cytometric dot plots of anti-CD80 antibody labeling of leukocytes in blood from healthy dogs. (A) Three different cell populations were identified on a forward and side scatter plot, corresponding to neutrophils, monocytes, and lymphocytes. The cells were double-labeled with anti-CD80-APC and -CD14-PE antibodies, with CD14 being used as a monocyte marker. CD80 was only expressed on CD14 + monocytes and CD14 − neutrophils but not lymphocytes (CD80 − /CD14 − ). The few CD14 + and CD14 − cells in the neutrophil and monocyte gates likely represent low numbers of monocytes and neutrophils in the respective gates. The CD80 − /CD14 − cells in neutrophil and monocyte gates could be large lymphocytes (representative result from 1 of 4 dogs). (B) Triple-labeling of dog leukocytes with CD80-APC, CD21-PE and CD5-FITC shows that CD21 + B cells and CD5 + T cells are CD80 − (representative result from 1 of 3 dogs). All leukocyte events were combined for analysis vs. splitting the events into different gates based on forward and side scatter.

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Flow cytometric dot plots of anti-CD80 antibody labeling of leukocytes in blood from healthy dogs. (A) Three different cell populations were identified on a forward and side scatter plot, corresponding to neutrophils, monocytes, and lymphocytes. The cells were double-labeled with anti-CD80-APC and -CD14-PE antibodies, with CD14 being used as a monocyte marker. CD80 was only expressed on CD14 + monocytes and CD14 − neutrophils but not lymphocytes (CD80 − /CD14 − ). The few CD14 + and CD14 − cells in the neutrophil and monocyte gates likely represent low numbers of monocytes and neutrophils in the respective gates. The CD80 − /CD14 − cells in neutrophil and monocyte gates could be large lymphocytes (representative result from 1 of 4 dogs). (B) Triple-labeling of dog leukocytes with CD80-APC, CD21-PE and CD5-FITC shows that CD21 + B cells and CD5 + T cells are CD80 − (representative result from 1 of 3 dogs). All leukocyte events were combined for analysis vs. splitting the events into different gates based on forward and side scatter.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Antibody Labeling, Labeling, Marker

Labeling of monocytes, B cells, T cells, and neutrophils isolated from the blood of healthy dogs (representative results from 1 of 2–3 dogs for each cell type) with the anti-CD80 antibody. (A-C) Monocytes, B cells, and T cells were isolated from peripheral blood mononuclear cells (PBMCs) using immunomagnetic beads and anti-CD14-PE, -CD21-FITC, and -CD5-FITC antibodies, respectively. The first panel shows the forward and side scatter events in PBMCs while the second panel shows the forward and side scatter events of isolated cells. The third panel is a fluorescent quadrant plot of double-labeled cells after adding the anti-CD80-APC antibody. The fourth panel shows a representative modified Wright’s-stained image of a cytospin smear of the isolated cells on which 100-cell differential cell counts were done (scale bar = 20 μm). (A) CD14-PE-isolated cells were mostly CD80 + monocytes (84% of a differential cell count), with a few contaminating neutrophils (10%) that were weakly CD14 + (arrows, third and fourth panels). A few monocytes had cytoplasmic vacuoles (fourth panel). Lymphocytes were negative for CD80 − /CD14 − (lower left quadrant, third panel, 6%). (B) CD21-FITC-isolated cells were mostly lymphocytes, which were CD80 − (third panel). Lymphocytes were primarily small cells, some of which had clefted or convoluted nuclei (variants of normal), with a few small or large reactive forms (fourth panel). (C) CD5-FITC-isolated cells were mostly lymphocytes, which were CD80 − (third panel). Lymphocytes were small cells with a few large or reactive forms. Several lymphocytes had a few clear cytoplasmic vacuoles, which could be due to the isolation procedure (fourth panel). (D) Neutrophils were isolated from the 1.077/1.119 interface of the double-density gradient used to obtain PBMCs and were single-labeled with the anti-CD80-APC antibody. The first panel shows a forward vs. side scatter plot of the isolated neutrophils and the second panel is a CD80 fluorescence vs. side scatter dot plot (blue) with overlaid hamster-APC isotype (red), showing neutrophils are CD80 + . The third panel shows a representative modified Wright’s-stained image of a cytospin smear of the isolated cells, which were primarily segmented neutrophils. The vacuolated cytoplasm in one neutrophil is likely an artifact of the isolation procedure (scale bar = 20 μm).

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Labeling of monocytes, B cells, T cells, and neutrophils isolated from the blood of healthy dogs (representative results from 1 of 2–3 dogs for each cell type) with the anti-CD80 antibody. (A-C) Monocytes, B cells, and T cells were isolated from peripheral blood mononuclear cells (PBMCs) using immunomagnetic beads and anti-CD14-PE, -CD21-FITC, and -CD5-FITC antibodies, respectively. The first panel shows the forward and side scatter events in PBMCs while the second panel shows the forward and side scatter events of isolated cells. The third panel is a fluorescent quadrant plot of double-labeled cells after adding the anti-CD80-APC antibody. The fourth panel shows a representative modified Wright’s-stained image of a cytospin smear of the isolated cells on which 100-cell differential cell counts were done (scale bar = 20 μm). (A) CD14-PE-isolated cells were mostly CD80 + monocytes (84% of a differential cell count), with a few contaminating neutrophils (10%) that were weakly CD14 + (arrows, third and fourth panels). A few monocytes had cytoplasmic vacuoles (fourth panel). Lymphocytes were negative for CD80 − /CD14 − (lower left quadrant, third panel, 6%). (B) CD21-FITC-isolated cells were mostly lymphocytes, which were CD80 − (third panel). Lymphocytes were primarily small cells, some of which had clefted or convoluted nuclei (variants of normal), with a few small or large reactive forms (fourth panel). (C) CD5-FITC-isolated cells were mostly lymphocytes, which were CD80 − (third panel). Lymphocytes were small cells with a few large or reactive forms. Several lymphocytes had a few clear cytoplasmic vacuoles, which could be due to the isolation procedure (fourth panel). (D) Neutrophils were isolated from the 1.077/1.119 interface of the double-density gradient used to obtain PBMCs and were single-labeled with the anti-CD80-APC antibody. The first panel shows a forward vs. side scatter plot of the isolated neutrophils and the second panel is a CD80 fluorescence vs. side scatter dot plot (blue) with overlaid hamster-APC isotype (red), showing neutrophils are CD80 + . The third panel shows a representative modified Wright’s-stained image of a cytospin smear of the isolated cells, which were primarily segmented neutrophils. The vacuolated cytoplasm in one neutrophil is likely an artifact of the isolation procedure (scale bar = 20 μm).

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Labeling, Isolation, Modification, Staining, Cell Counting, Fluorescence

Percentage differential cell counts (mean and range) from modified Wright’s-stained cytospin smears of isolated monocytes, B cells, T cells, and neutrophils.

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Percentage differential cell counts (mean and range) from modified Wright’s-stained cytospin smears of isolated monocytes, B cells, T cells, and neutrophils.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Modification, Isolation

Positive labeling with the anti-CD80 antibody in tumor cells in dogs with hematopoietic neoplasms.

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Positive labeling with the anti-CD80 antibody in tumor cells in dogs with hematopoietic neoplasms.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Labeling

Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. CD3−CD5−CD21− cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).

Journal: Frontiers in immunology

Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.

doi: 10.3389/fimmu.2018.00841

Figure Lengend Snippet: Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. CD3−CD5−CD21− cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).

Article Snippet: Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier.

Techniques: Purification, Isolation

Figure 6 | Comparison of the proliferative capacity in CD3−CD5−CD21− non-B, non-T (CD5−), CD3+CD5dimCD21− (CD5dim), and CD3+CD5brightCD21− (CD5bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.

Journal: Frontiers in immunology

Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.

doi: 10.3389/fimmu.2018.00841

Figure Lengend Snippet: Figure 6 | Comparison of the proliferative capacity in CD3−CD5−CD21− non-B, non-T (CD5−), CD3+CD5dimCD21− (CD5dim), and CD3+CD5brightCD21− (CD5bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.

Techniques: Comparison, Staining, Cell Culture, Negative Control

Review

anti dog cd5 pacific bluetm ykix322 3 bio rad mca1037pb mouse anti dog cd94 alexa fluor 647 (Bio-Rad)

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Diagnostic Assay:

Antibody Labeling:

Labeling:

Marker:

Isolation:

Modification:

Staining:

Cell Counting:

Fluorescence:

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