Journal: BMC Genomics
Article Title: Single-cell RNA-seq mapping of chicken peripheral blood leukocytes
doi: 10.1186/s12864-024-10044-4
Figure Lengend Snippet: Marker genes used for primary annotation of cell types among chicken leukocytes
Article Snippet: Cells were suspended in PBS supplemented with 2% foetal bovine serum (Gibco® #10,082,147, ThermoFisher Scientific) and 1 mM EDTA and the number of thrombocytes in the samples were reduced by immunomagnetic cell separation using the EasySep PE Positive Selection Kit II (#17,684, StemCell Technologies) and PE-conjugated mouse monoclonal antibody to chicken CD41/61 (clone 11C3, #AM05550PE-N, OriGene) according to the StemCell Technologies depletion protocol no. 28,898 as previously described for chicken leukocytes [ ].
Techniques: Marker, Expressing, Binding Assay