other:
Incubation:Article Title: Differential Effects of Opuntia ficus-indica and Opuntia stricta var. dillenii Extracts on Liver Steatosis in a Murine Dietary Model
Article Snippet: Proteins were separated via electrophoresis on 4–15% SDS-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Merck, Darmstadt, Germany). .. Membranes were incubated with 4% BSA for 1.5 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies (1:1000) against FATP2 (Santa Cruz Biotech, Dallas, TX, USA), FATP5 (Lifespan Bioscience, Lynnwood, DC, USA), CD36 (Cell Signaling Danvers, MA, USA), AQP9 (Santa Cruz Biotech, Dallas, TX, USA), ChREBP (Novus Biologicals, Centennial, CO, USA), SREBP-1c (Abcam, Cambridge, UK), pACC (1Cell Signaling Danvers, MA, USA), ACC (Cell Signaling Danvers, MA, USA), FAS (Abcam, Cambridge, UK), pSIK1 (Thermo Fisher Scientific Inc., Rockford, IL, USA), SIK1 (Novus Biologicals, Centennial, CO, USA), DGAT2 (Abcam, Cambridge, UK), pAMPK (Cell Signaling Danvers, MA, USA), AMPK (Cell Signaling Danvers, MA, USA), SIRT1 (Abcam, Cambridge, UK), acetylated lysine (Cell Signaling Danvers, MA, USA), PGC-1α (1Novus Biologicals, Centennial, CO, USA), NRF1 (Abcam, Cambridge, UK), TFAM (Santa Cruz Biotech, Dallas, TX, USA), CPT1A (Abcam, Cambridge, UK), UCP2 (Santa Cruz Biotech, Dallas, TX, USA), ABHD5 (Invitrogen, Waltham, MA, USA), ATGL (Cell Signaling Danvers, MA, USA), MTP (Abcam, Cambridge, UK), pULK1 (Ser 757; Cell Signaling Danvers, MA, USA), ULK1 (Abcam, Cambridge, UK), LC3 (Cell Signaling Danvers, MA, USA), p62 (Abcam, Cambridge, UK), GAPDH (Abcam, Cambridge, UK), and histone H3 (Abcam, Cambridge, UK). .. Afterwards, membranes were incubated for 2 h at room temperature with the corresponding secondary antibodies (1:5000): polyclonal anti-mouse for AQP9, CPT1A, UCP2, p62, and GAPDH; anti-rabbit for FATP5, CD36, ChREBP, SREBP-1c, pACC, ACC, FAS, pSIK1, SIK1, DGAT2, pAMPK, AMPK, SIRT1, acetylated lysine, PGC-1α, NRF1, ABHD5, ATGL, MTP, pULK1, ULK1, LC3, and histone H3; and anti-goat for FATP2 and TFAM (all from Santa Cruz Biotech, Dallas, TX, USA).
Article Title: Differential Effects of Opuntia ficus-indica and Opuntia stricta var. dillenii Extracts on Liver Steatosis in a Murine Dietary Model.
Article Snippet: Proteins were separated via electrophoresis on 4–15% SDS-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Merck, Darmstadt, Germany). .. Membranes were incubated with 4% BSA for 1.5 h at room temperature, followed by overnight incubation at 4 ◦C with primary antibodies (1:1000) against FATP2 (Santa Cruz Biotech, Dallas, TX, USA), FATP5 (Lifespan Bioscience, Lynnwood, DC, USA), CD36 (Cell Signaling Danvers, MA, USA), AQP9 (Santa Cruz Biotech, Dallas, TX, USA), ChREBP (Novus Biologicals, Centennial, CO, USA), SREBP-1c (Abcam, Cambridge, UK), pACC (1Cell Signaling Danvers, MA, https://doi.org/10.3390/nu18030420 USA), ACC (Cell Signaling Danvers, MA, USA), FAS (Abcam, Cambridge, UK), pSIK1 (Thermo Fisher Scientific Inc., Rockford, IL, USA), SIK1 (Novus Biologicals, Centennial, CO, USA), DGAT2 (Abcam, Cambridge, UK), pAMPK (Cell Signaling Danvers, MA, USA), AMPK (Cell Signaling Danvers, MA, USA), SIRT1 (Abcam, Cambridge, UK), acetylated lysine (Cell Signaling Danvers, MA, USA), PGC-1α (1Novus Biologicals, Centennial, CO, USA), NRF1 (Abcam, Cambridge, UK), TFAM (Santa Cruz Biotech, Dallas, TX, USA), CPT1A (Abcam, Cambridge, UK), UCP2 (Santa Cruz Biotech, Dallas, TX, USA), ABHD5 (Invitrogen, Waltham, MA, USA), ATGL (Cell Signaling Danvers, MA, USA), MTP (Abcam, Cambridge, UK), pULK1 (Ser 757; Cell Signaling Danvers, MA, USA), ULK1 (Abcam, Cambridge, UK), LC3 (Cell Signaling Danvers, MA, USA), p62 (Abcam, Cambridge, UK), GAPDH (Abcam, Cambridge, UK), and histone H3 (Abcam, Cambridge, UK). .. Afterwards, membranes were incubated for 2 h at room temperature with the corresponding secondary antibodies (1:5000): polyclonal anti-mouse for AQP9, CPT1A, UCP2, p62, and GAPDH; anti-rabbit for FATP5, CD36, ChREBP, SREBP-1c, pACC, ACC, FAS, pSIK1, SIK1, DGAT2, pAMPK, AMPK, SIRT1, acetylated lysine, PGC-1α, NRF1, ABHD5, ATGL, MTP, pULK1, ULK1, LC3, and histone H3; and anti-goat for FATP2 and TFAM (all from Santa Cruz Biotech, Dallas, TX, USA).
Article Title: Elevated CD36 drives lymph node metastasis of breast cancer by activating Hippo-YAP signaling-mediated anoikis resistance
Article Snippet: Proteins were separated by SDS-PAGE and transferred to 0.45 μm polyvinyl difluoride (PVDF) membranes (Millipore) according to standard immunoblotting protocols. .. After blocking with 5% of defatted milk in TBST (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween-20) for 1 h at room temperature, the membranes were incubated with the following primary antibodies: CD36 (#14347, 1:1000), Caspase 9 (#9502, 1:1000), Cytochrome-C (#4272, 1:1000), YAP (#14074, 1:1000), p-YAP (Ser127) (#4911, 1:1000) from Cell Signaling Technology; Cox-IV (ab14744, 1:5000), Histone H3 (ab1791, 1:5000), β-tublin (ab179513, 1:5000), CTGF (ab6992, 1:1000) from Abcam; Bcl-2 (sc-7382, 1:1000), Bak (sc-832, 1:1000), Bax (sc-6236, 1:1000) from Santa Cruz; Bim (AF1573, 1:1000) from Beyotime; Bid (MB0118, 1:1000) from bioworld; β-actin (A5441, 1:5000) from Sigma-Aldrich (St. Louis, USA); Ubiquitin Rabbit mAb (A19686, 1:1000) from ABclonal; Lamin B1 Ab (AB0054, 1:5000) from Abways. .. HRP-conjugated anti-rabbit IgG (Cell Signaling Tech, #7074) and anti-mouse IgG (Sigma-Aldrich, AP308P) were used as secondary antibodies.
Article Title: Elevated CD36 drives lymph node metastasis of breast cancer by activating Hippo-YAP signaling-mediated anoikis resistance.
Article Snippet: Proteins were separated by SDS-PAGE and transferred to 0.45 μm polyvinyl difluoride (PVDF) membranes (Millipore) according to standard immunoblotting protocols. .. After blocking with 5% of defatted milk in TBST (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween-20) for 1 hr at room temperature, the membranes were incubated with the following primary antibodies: CD36 (#14347, 1:1000), Caspase 9 (#9502, 1:1000), Cytochrome-C (#4272, 1:1000), YAP (#14074, 1:1000), p-YAP (Ser127) (#4911, 1:1000) from Cell Signaling Technology; Cox-IV (ab14744, 1:5000), Histone H3 (ab1791, 1:5000), β-tublin (ab179513, 1:5000), CTGF (ab6992, 1:1000) from Abcam; Bcl-2 (sc-7382, 1:1000), Bak (sc-832, 1:1000), Bax (sc-6236, 1:1000) from Santa Cruz; Bim (AF1573, 1:1000) from Beyotime; Bid (MB0118, 1:1000) from bioworld; β-actin (A5441, 1:5000) from Sigma-Aldrich (St. Louis, USA);Ubiquitin Rabbit mAb (A19686, 1:1000) from ABclonal; Lamin B1 Ab (AB0054, 1:5000) from Abways. .. HRP-conjugated anti-rabbit IgG (Cell Signaling Tech, #7074) and anti-mouse IgG (Sigma-Aldrich, AP308P) were used as secondary antibodies.
Pyrolysis Gas Chromatography:Article Title: Differential Effects of Opuntia ficus-indica and Opuntia stricta var. dillenii Extracts on Liver Steatosis in a Murine Dietary Model
Article Snippet: Proteins were separated via electrophoresis on 4–15% SDS-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Merck, Darmstadt, Germany). .. Membranes were incubated with 4% BSA for 1.5 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies (1:1000) against FATP2 (Santa Cruz Biotech, Dallas, TX, USA), FATP5 (Lifespan Bioscience, Lynnwood, DC, USA), CD36 (Cell Signaling Danvers, MA, USA), AQP9 (Santa Cruz Biotech, Dallas, TX, USA), ChREBP (Novus Biologicals, Centennial, CO, USA), SREBP-1c (Abcam, Cambridge, UK), pACC (1Cell Signaling Danvers, MA, USA), ACC (Cell Signaling Danvers, MA, USA), FAS (Abcam, Cambridge, UK), pSIK1 (Thermo Fisher Scientific Inc., Rockford, IL, USA), SIK1 (Novus Biologicals, Centennial, CO, USA), DGAT2 (Abcam, Cambridge, UK), pAMPK (Cell Signaling Danvers, MA, USA), AMPK (Cell Signaling Danvers, MA, USA), SIRT1 (Abcam, Cambridge, UK), acetylated lysine (Cell Signaling Danvers, MA, USA), PGC-1α (1Novus Biologicals, Centennial, CO, USA), NRF1 (Abcam, Cambridge, UK), TFAM (Santa Cruz Biotech, Dallas, TX, USA), CPT1A (Abcam, Cambridge, UK), UCP2 (Santa Cruz Biotech, Dallas, TX, USA), ABHD5 (Invitrogen, Waltham, MA, USA), ATGL (Cell Signaling Danvers, MA, USA), MTP (Abcam, Cambridge, UK), pULK1 (Ser 757; Cell Signaling Danvers, MA, USA), ULK1 (Abcam, Cambridge, UK), LC3 (Cell Signaling Danvers, MA, USA), p62 (Abcam, Cambridge, UK), GAPDH (Abcam, Cambridge, UK), and histone H3 (Abcam, Cambridge, UK). .. Afterwards, membranes were incubated for 2 h at room temperature with the corresponding secondary antibodies (1:5000): polyclonal anti-mouse for AQP9, CPT1A, UCP2, p62, and GAPDH; anti-rabbit for FATP5, CD36, ChREBP, SREBP-1c, pACC, ACC, FAS, pSIK1, SIK1, DGAT2, pAMPK, AMPK, SIRT1, acetylated lysine, PGC-1α, NRF1, ABHD5, ATGL, MTP, pULK1, ULK1, LC3, and histone H3; and anti-goat for FATP2 and TFAM (all from Santa Cruz Biotech, Dallas, TX, USA).
Article Title: Differential Effects of Opuntia ficus-indica and Opuntia stricta var. dillenii Extracts on Liver Steatosis in a Murine Dietary Model.
Article Snippet: Proteins were separated via electrophoresis on 4–15% SDS-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Merck, Darmstadt, Germany). .. Membranes were incubated with 4% BSA for 1.5 h at room temperature, followed by overnight incubation at 4 ◦C with primary antibodies (1:1000) against FATP2 (Santa Cruz Biotech, Dallas, TX, USA), FATP5 (Lifespan Bioscience, Lynnwood, DC, USA), CD36 (Cell Signaling Danvers, MA, USA), AQP9 (Santa Cruz Biotech, Dallas, TX, USA), ChREBP (Novus Biologicals, Centennial, CO, USA), SREBP-1c (Abcam, Cambridge, UK), pACC (1Cell Signaling Danvers, MA, https://doi.org/10.3390/nu18030420 USA), ACC (Cell Signaling Danvers, MA, USA), FAS (Abcam, Cambridge, UK), pSIK1 (Thermo Fisher Scientific Inc., Rockford, IL, USA), SIK1 (Novus Biologicals, Centennial, CO, USA), DGAT2 (Abcam, Cambridge, UK), pAMPK (Cell Signaling Danvers, MA, USA), AMPK (Cell Signaling Danvers, MA, USA), SIRT1 (Abcam, Cambridge, UK), acetylated lysine (Cell Signaling Danvers, MA, USA), PGC-1α (1Novus Biologicals, Centennial, CO, USA), NRF1 (Abcam, Cambridge, UK), TFAM (Santa Cruz Biotech, Dallas, TX, USA), CPT1A (Abcam, Cambridge, UK), UCP2 (Santa Cruz Biotech, Dallas, TX, USA), ABHD5 (Invitrogen, Waltham, MA, USA), ATGL (Cell Signaling Danvers, MA, USA), MTP (Abcam, Cambridge, UK), pULK1 (Ser 757; Cell Signaling Danvers, MA, USA), ULK1 (Abcam, Cambridge, UK), LC3 (Cell Signaling Danvers, MA, USA), p62 (Abcam, Cambridge, UK), GAPDH (Abcam, Cambridge, UK), and histone H3 (Abcam, Cambridge, UK). .. Afterwards, membranes were incubated for 2 h at room temperature with the corresponding secondary antibodies (1:5000): polyclonal anti-mouse for AQP9, CPT1A, UCP2, p62, and GAPDH; anti-rabbit for FATP5, CD36, ChREBP, SREBP-1c, pACC, ACC, FAS, pSIK1, SIK1, DGAT2, pAMPK, AMPK, SIRT1, acetylated lysine, PGC-1α, NRF1, ABHD5, ATGL, MTP, pULK1, ULK1, LC3, and histone H3; and anti-goat for FATP2 and TFAM (all from Santa Cruz Biotech, Dallas, TX, USA).
Blocking Assay:Article Title: Elevated CD36 drives lymph node metastasis of breast cancer by activating Hippo-YAP signaling-mediated anoikis resistance
Article Snippet: Proteins were separated by SDS-PAGE and transferred to 0.45 μm polyvinyl difluoride (PVDF) membranes (Millipore) according to standard immunoblotting protocols. .. After blocking with 5% of defatted milk in TBST (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween-20) for 1 h at room temperature, the membranes were incubated with the following primary antibodies: CD36 (#14347, 1:1000), Caspase 9 (#9502, 1:1000), Cytochrome-C (#4272, 1:1000), YAP (#14074, 1:1000), p-YAP (Ser127) (#4911, 1:1000) from Cell Signaling Technology; Cox-IV (ab14744, 1:5000), Histone H3 (ab1791, 1:5000), β-tublin (ab179513, 1:5000), CTGF (ab6992, 1:1000) from Abcam; Bcl-2 (sc-7382, 1:1000), Bak (sc-832, 1:1000), Bax (sc-6236, 1:1000) from Santa Cruz; Bim (AF1573, 1:1000) from Beyotime; Bid (MB0118, 1:1000) from bioworld; β-actin (A5441, 1:5000) from Sigma-Aldrich (St. Louis, USA); Ubiquitin Rabbit mAb (A19686, 1:1000) from ABclonal; Lamin B1 Ab (AB0054, 1:5000) from Abways. .. HRP-conjugated anti-rabbit IgG (Cell Signaling Tech, #7074) and anti-mouse IgG (Sigma-Aldrich, AP308P) were used as secondary antibodies.
Article Title: Elevated CD36 drives lymph node metastasis of breast cancer by activating Hippo-YAP signaling-mediated anoikis resistance.
Article Snippet: Proteins were separated by SDS-PAGE and transferred to 0.45 μm polyvinyl difluoride (PVDF) membranes (Millipore) according to standard immunoblotting protocols. .. After blocking with 5% of defatted milk in TBST (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween-20) for 1 hr at room temperature, the membranes were incubated with the following primary antibodies: CD36 (#14347, 1:1000), Caspase 9 (#9502, 1:1000), Cytochrome-C (#4272, 1:1000), YAP (#14074, 1:1000), p-YAP (Ser127) (#4911, 1:1000) from Cell Signaling Technology; Cox-IV (ab14744, 1:5000), Histone H3 (ab1791, 1:5000), β-tublin (ab179513, 1:5000), CTGF (ab6992, 1:1000) from Abcam; Bcl-2 (sc-7382, 1:1000), Bak (sc-832, 1:1000), Bax (sc-6236, 1:1000) from Santa Cruz; Bim (AF1573, 1:1000) from Beyotime; Bid (MB0118, 1:1000) from bioworld; β-actin (A5441, 1:5000) from Sigma-Aldrich (St. Louis, USA);Ubiquitin Rabbit mAb (A19686, 1:1000) from ABclonal; Lamin B1 Ab (AB0054, 1:5000) from Abways. .. HRP-conjugated anti-rabbit IgG (Cell Signaling Tech, #7074) and anti-mouse IgG (Sigma-Aldrich, AP308P) were used as secondary antibodies.
Ubiquitin Proteomics:Article Title: Elevated CD36 drives lymph node metastasis of breast cancer by activating Hippo-YAP signaling-mediated anoikis resistance
Article Snippet: Proteins were separated by SDS-PAGE and transferred to 0.45 μm polyvinyl difluoride (PVDF) membranes (Millipore) according to standard immunoblotting protocols. .. After blocking with 5% of defatted milk in TBST (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween-20) for 1 h at room temperature, the membranes were incubated with the following primary antibodies: CD36 (#14347, 1:1000), Caspase 9 (#9502, 1:1000), Cytochrome-C (#4272, 1:1000), YAP (#14074, 1:1000), p-YAP (Ser127) (#4911, 1:1000) from Cell Signaling Technology; Cox-IV (ab14744, 1:5000), Histone H3 (ab1791, 1:5000), β-tublin (ab179513, 1:5000), CTGF (ab6992, 1:1000) from Abcam; Bcl-2 (sc-7382, 1:1000), Bak (sc-832, 1:1000), Bax (sc-6236, 1:1000) from Santa Cruz; Bim (AF1573, 1:1000) from Beyotime; Bid (MB0118, 1:1000) from bioworld; β-actin (A5441, 1:5000) from Sigma-Aldrich (St. Louis, USA); Ubiquitin Rabbit mAb (A19686, 1:1000) from ABclonal; Lamin B1 Ab (AB0054, 1:5000) from Abways. .. HRP-conjugated anti-rabbit IgG (Cell Signaling Tech, #7074) and anti-mouse IgG (Sigma-Aldrich, AP308P) were used as secondary antibodies.
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