Review



human cd34 microbead kit  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Miltenyi Biotec human cd34 microbead kit
    Human Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit%2C+human/pm42869019-363-11-15
    Average 97 stars, based on 1655 article reviews
    human cd34 microbead kit - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Article Title: Temporal Lysophosphatidic Acid Supplementation Enhances Megakaryocyte Differentiation and Platelet Production From Human Hematopoietic Progenitors
    Article Snippet: Umbilical cord blood was diluted with phosphate buffer saline (PBS) at a 1:1 ratio, and gently layered onto Lymphoprep TM (STEMCELL Technologies, Canada), and then centrifuged at 2,000 rpm for 20 min with break. .. Mononuclear cells located between plasma and Lymphoprep TM layers were harvested, washed twice with PBS, and centrifuged at 1,500 rpm for 5 min. HSPCs were isolated from mononuclear cells using a CD34 MicroBead Kit (Miltenyi Biotec, Germany). ..

    Article Title: Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.
    Article Snippet: Cells from a leukapheresis sample were isolated using Ficoll-Paque PLUS (GE Healthcare) and density centrifugation, followed by NH4Cl lysis to remove red blood cells. .. A secondary isolation step was completed using CD34 MicroBead Kit (Miltenyi Biotec) performed according to the manufacturer’s protocol for positive selection of CD34+ cells from the mononuclear population. .. Isolated CD34+ cells were cultured in expansion medium (Stemspan SFEM (Stem Cell Technologies 09650), 50ng/mL rhFLt3L (R&D 308-FKN), 50ng/mL rhSCF (R&D 255-SC), 10ng/mL rhIL-3 (R&D 203-IL), 10ng/mL rhIL-6 (R&D 206-IL), 35nM UM171 and 500nM Stemreginin) with or without SA for 4 and 7 days.

    Selection:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.
    Article Snippet: Cells from a leukapheresis sample were isolated using Ficoll-Paque PLUS (GE Healthcare) and density centrifugation, followed by NH4Cl lysis to remove red blood cells. .. A secondary isolation step was completed using CD34 MicroBead Kit (Miltenyi Biotec) performed according to the manufacturer’s protocol for positive selection of CD34+ cells from the mononuclear population. .. Isolated CD34+ cells were cultured in expansion medium (Stemspan SFEM (Stem Cell Technologies 09650), 50ng/mL rhFLt3L (R&D 308-FKN), 50ng/mL rhSCF (R&D 255-SC), 10ng/mL rhIL-3 (R&D 203-IL), 10ng/mL rhIL-6 (R&D 206-IL), 35nM UM171 and 500nM Stemreginin) with or without SA for 4 and 7 days.

    Magnetic Cell Separation:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Cell Culture:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Article Title: Foxs1-mediated transformation of CD34 + fibroblast to myCAFs promotes tumor growth.
    Article Snippet: .. These cells were purified with a Cd34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and cultured in S/P cell culture medium. ..

    Ex Vivo:

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch
    Article Snippet: .. CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ]. .. pTLCV2-sgRNA-Zeb1-up-exon4 (targeting Zeb1 intron 3, and containing a Cas9 expression cassette) and pLentiGuide-Hygro-sgZeb1-downEx6 (targeting Zeb1 intron 6) viral plasmids were generated by inserting annealed sgRNA primers (Table ) into pTLCV2 (Addgene #87360) and pLentiGuide-Hygro (Addgene #139462), respectively.

    Gradient Centrifugation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Purification:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Foxs1-mediated transformation of CD34 + fibroblast to myCAFs promotes tumor growth.
    Article Snippet: .. These cells were purified with a Cd34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and cultured in S/P cell culture medium. ..

    Article Title: Modular Microfluidic Device for Intensified CAR T Cell Expansion
    Article Snippet: .. CAR T cells were purified using a CD34 MicroBead Kit and LS Columns (Miltenyi Biotec Ltd., UK) [37]. .. Purified CAR T cells were co-cultured at a 1:1 (CAR-T cell: target cell) ratio with GFP + , CD19 + NALM6 target cells in poly-L-lysine-coated 96-well plates (Sigma Aldrich Merck, UK; Sarstedt AG & Co. KG, Germany).

    Immunostaining:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Cell Isolation:

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Clinical Proteomics:

    Article Title: Temporal Lysophosphatidic Acid Supplementation Enhances Megakaryocyte Differentiation and Platelet Production From Human Hematopoietic Progenitors
    Article Snippet: Umbilical cord blood was diluted with phosphate buffer saline (PBS) at a 1:1 ratio, and gently layered onto Lymphoprep TM (STEMCELL Technologies, Canada), and then centrifuged at 2,000 rpm for 20 min with break. .. Mononuclear cells located between plasma and Lymphoprep TM layers were harvested, washed twice with PBS, and centrifuged at 1,500 rpm for 5 min. HSPCs were isolated from mononuclear cells using a CD34 MicroBead Kit (Miltenyi Biotec, Germany). ..



    Similar Products

    97
    Miltenyi Biotec human cd34 microbead kit
    Human Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit%2C+human/pm42869019-363-11-15
    Average 97 stars, based on 1 article reviews
    human cd34 microbead kit - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd34 microbead kit
    Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit%2C+human/pm42828480-65-23-26
    Average 97 stars, based on 1 article reviews
    cd34 microbead kit - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec isotonic ammonium chloride solution
    Isotonic Ammonium Chloride Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit+UltraPure%2C+human/10__1016_slash_j__bict__2026__100095-83-5-16
    Average 96 stars, based on 1 article reviews
    isotonic ammonium chloride solution - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd34 microbead kit ultrapure, human
    Cd34 Microbead Kit Ultrapure, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit+UltraPure%2C+human/custom%40130-100-453%4042603588
    Average 96 stars, based on 1 article reviews
    cd34 microbead kit ultrapure, human - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec cd34 microbead kit, human
    Cd34 Microbead Kit, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit%2C+human/custom%40130-046-703%4010%2E1158%2F2643-3230%2Ebcd-25-0230
    Average 97 stars, based on 1 article reviews
    cd34 microbead kit, human - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec magnetic selection
    Magnetic Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit+UltraPure%2C+human/pm42486681-84-6-8
    Average 96 stars, based on 1 article reviews
    magnetic selection - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd34 microbead kit ultrapure
    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed <t>progenitor</t> <t>cells</t> (A) Flow cytometric analysis of <t>CD34,</t> c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted <t>CD34</t> + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .
    Cd34 Microbead Kit Ultrapure, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit+UltraPure%2C+human/pmc13400188-422-23-27
    Average 96 stars, based on 1 article reviews
    cd34 microbead kit ultrapure - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results


    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Journal: Cell Reports Medicine

    Article Title: ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development

    doi: 10.1016/j.xcrm.2026.102879

    Figure Lengend Snippet: B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Article Snippet: Leukocytes from UCB and mPB were first purified using the Erythrocyte Sedimentation Kit II (Miltenyi), followed by CD34 + cell enrichment with the CD34 MicroBead Kit UltraPure (Miltenyi) per manufacturer’s instructions.

    Techniques: Expressing