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cd32a  (Sino Biological)


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    Structured Review

    Sino Biological cd32a
    Cd32a, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd32a/Human+CD32a+%2F+FCGR2A+Protein/10__1158_slash_1535___7163__mct___25___1153-106-40-42
    Average 94 stars, based on 13 article reviews
    cd32a - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Non-Glycosylated, Legumain-Cleavable ISACs Drive Potent Anti-Tumor Immunotherapy via a Bystander Effect
    Article Snippet: .. Following incubation, supernatants were collected, and the release of E104 was quantified by LC-MS. OpenSPR binding analysis Histidine-tagged Fcγ receptor proteins were obtained from commercial suppliers: CD64 (FcγRI, Sino Biological, Cat. No. 10256-H08S-100), CD16a (FcγRIIIa, Invitrogen, Cat. No. RP87975), and CD32a (FcγRIIa, Sino Biological, Cat. No. 10374-H08H1-100). .. Binding assays were performed using a 2-channel OpenSPR (or OpenSPR-XT) instrument (Nicoya Lifesciences, Kitchener, ON, Canada) with a carboxylfunctionalized sensor chip (Carboxyl Chip, Nicoya, Cat. No. SEN-AU-100-10-HC-COOH) in PBS running buffer.

    Liquid Chromatography:

    Article Title: Non-Glycosylated, Legumain-Cleavable ISACs Drive Potent Anti-Tumor Immunotherapy via a Bystander Effect
    Article Snippet: .. Following incubation, supernatants were collected, and the release of E104 was quantified by LC-MS. OpenSPR binding analysis Histidine-tagged Fcγ receptor proteins were obtained from commercial suppliers: CD64 (FcγRI, Sino Biological, Cat. No. 10256-H08S-100), CD16a (FcγRIIIa, Invitrogen, Cat. No. RP87975), and CD32a (FcγRIIa, Sino Biological, Cat. No. 10374-H08H1-100). .. Binding assays were performed using a 2-channel OpenSPR (or OpenSPR-XT) instrument (Nicoya Lifesciences, Kitchener, ON, Canada) with a carboxylfunctionalized sensor chip (Carboxyl Chip, Nicoya, Cat. No. SEN-AU-100-10-HC-COOH) in PBS running buffer.

    Binding Assay:

    Article Title: Non-Glycosylated, Legumain-Cleavable ISACs Drive Potent Anti-Tumor Immunotherapy via a Bystander Effect
    Article Snippet: .. Following incubation, supernatants were collected, and the release of E104 was quantified by LC-MS. OpenSPR binding analysis Histidine-tagged Fcγ receptor proteins were obtained from commercial suppliers: CD64 (FcγRI, Sino Biological, Cat. No. 10256-H08S-100), CD16a (FcγRIIIa, Invitrogen, Cat. No. RP87975), and CD32a (FcγRIIa, Sino Biological, Cat. No. 10374-H08H1-100). .. Binding assays were performed using a 2-channel OpenSPR (or OpenSPR-XT) instrument (Nicoya Lifesciences, Kitchener, ON, Canada) with a carboxylfunctionalized sensor chip (Carboxyl Chip, Nicoya, Cat. No. SEN-AU-100-10-HC-COOH) in PBS running buffer.

    Analysis:

    Article Title: Non-Glycosylated, Legumain-Cleavable ISACs Drive Potent Anti-Tumor Immunotherapy via a Bystander Effect
    Article Snippet: .. Following incubation, supernatants were collected, and the release of E104 was quantified by LC-MS. OpenSPR binding analysis Histidine-tagged Fcγ receptor proteins were obtained from commercial suppliers: CD64 (FcγRI, Sino Biological, Cat. No. 10256-H08S-100), CD16a (FcγRIIIa, Invitrogen, Cat. No. RP87975), and CD32a (FcγRIIa, Sino Biological, Cat. No. 10374-H08H1-100). .. Binding assays were performed using a 2-channel OpenSPR (or OpenSPR-XT) instrument (Nicoya Lifesciences, Kitchener, ON, Canada) with a carboxylfunctionalized sensor chip (Carboxyl Chip, Nicoya, Cat. No. SEN-AU-100-10-HC-COOH) in PBS running buffer.

    other:

    Article Title: Anti-CTLA4 antibodies and methods of making and using the same
    Article Snippet: Protein binding was examined by surface plasmon resonance (SPR) analysis using a BiacoreTM T200 instrument (Biacore AB, Uppsala, Sweden) according to the manufacturer's guidelines.

    Article Title: Anti-CTLA4 antibodies and methods of making and using the same
    Article Snippet: Protein binding was examined by surface plasmon resonance (SPR) analysis using a BiacoreTM T200 instrument (Biacore AB, Uppsala, Sweden) according to the manufacturer's guidelines.

    Recombinant:

    Article Title: CA125 suppresses amatuximab immune-effector function and elevated serum levels are associated with reduced clinical response in first line mesothelioma patients
    Article Snippet: Jurkat-CD16a cells [25,000 in ADCC buffer (Promega)] were then added with or without CA125 to wells and plates were incubated for 5 hrs at 37°C/5% CO 2 in a humidified incubator and imaged (EVOS System). .. Recombinant human CD16a-, CD32a- and CD64a-biotin probes were purchased from Sino Biological Inc. (Beijing, China). .. Recombinant human FcRn was purchased from R&D Systems.

    Generated:

    Article Title: Tumor antigen CA125 suppresses antibody-dependent cellular cytotoxicity (ADCC) via direct antibody binding and suppressed Fc-γ receptor engagement
    Article Snippet: .. Human CD16a-, CD32a- and CD64a-biotin probes were purchased from Sino Biological Inc. Biotinylated CA125 or HSA were generated as previously described [ ]. .. 2.5×10 4 Jurkat or CHO cell transfectants expressing CD16a, 32a or 64a were added with or without sCA125 to wells in a total volume of 100 μL ADCC assay buffer (Promega).



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    Image Search Results


    Identification of potential biomarkers in ATC/PDTC. A , volcano plot of DEPs in ATC/PDTC versus PTC with |log 2 FC| ≥ 1 and p < 0.05. B , correlation analysis of FCGR2A with NET formation markers PADI4 across groups. C , single-cell RNA-seq data ( GSE232237 ) showing FCGR2A expression predominantly in macrophages, with the highest expression in ATC. D , correlation analysis of FCGR2A expression with M0 macrophages, NK cells, and B cells examined by Spearman analysis. E , representative figures of IHC analysis validation of FCGR2A, UBE2C, and NUBPL expression in ATC (n = 11), PDTC (n = 7), and PTC (n = 10) tissues. The numbers at the upper left corner of each IHC image (0–3) indicate the immunoreactivity score for the representative case. The IHC scores were fully plotted in the bar plots assessed by pairwise student’s t tests. ns, not significant; ∗ p < 0.05; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. ATC, anaplastic thyroid carcinoma; FCGR2A, Fc fragment of IgG receptor IIa; IHC, immunohistochemistry; log 2 FC, log 2 fold change; NK, natural killer; PDTC, poorly differentiated thyroid carcinoma; PTC, papillary thyroid carcinoma; PADI4, peptidyl arginine deiminase 4; NUBPL, nucleotide-binding protein-like; UBE2C, ubiquitin-conjugating enzyme E2 C.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Multiomics Profiling Reveals Distinct Immunosuppression and Metabolic Dysregulation in Aggressive Subtypes of Thyroid Cancer

    doi: 10.1016/j.mcpro.2026.101513

    Figure Lengend Snippet: Identification of potential biomarkers in ATC/PDTC. A , volcano plot of DEPs in ATC/PDTC versus PTC with |log 2 FC| ≥ 1 and p < 0.05. B , correlation analysis of FCGR2A with NET formation markers PADI4 across groups. C , single-cell RNA-seq data ( GSE232237 ) showing FCGR2A expression predominantly in macrophages, with the highest expression in ATC. D , correlation analysis of FCGR2A expression with M0 macrophages, NK cells, and B cells examined by Spearman analysis. E , representative figures of IHC analysis validation of FCGR2A, UBE2C, and NUBPL expression in ATC (n = 11), PDTC (n = 7), and PTC (n = 10) tissues. The numbers at the upper left corner of each IHC image (0–3) indicate the immunoreactivity score for the representative case. The IHC scores were fully plotted in the bar plots assessed by pairwise student’s t tests. ns, not significant; ∗ p < 0.05; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. ATC, anaplastic thyroid carcinoma; FCGR2A, Fc fragment of IgG receptor IIa; IHC, immunohistochemistry; log 2 FC, log 2 fold change; NK, natural killer; PDTC, poorly differentiated thyroid carcinoma; PTC, papillary thyroid carcinoma; PADI4, peptidyl arginine deiminase 4; NUBPL, nucleotide-binding protein-like; UBE2C, ubiquitin-conjugating enzyme E2 C.

    Article Snippet: Primary antibodies—including FCGR2A (RRID: AB_2246912 , Cat.15625-1-AP, Proteintech, 1:600 dilution), ubiquitin-conjugating enzyme E2 C (UBE2C) (RRID: AB_11232220 , Cat.66087-1-Ig, Proteintech, 1:500), and nucleotide-binding protein-like (NUBPL) (RRID: AB_2878402 , Cat.17393-1-AP, Proteintech, 1:200)—were diluted in antibody dilution buffer and applied to tissue sections.

    Techniques: Single Cell, RNA Sequencing, Expressing, Biomarker Discovery, Immunohistochemistry, Binding Assay, Ubiquitin Proteomics