Staining:Article Title: NK cells from COVID-19 positive patients exhibit enhanced cytotoxic activity upon NKG2A and KIR2DL1 blockade
Article Snippet: .. Peripheral blood samples from patients were directly stained with the following antibodies CD159a (FITC, Miltenyi), CD158e (APC, Miltenyi), CD314 (PE-VIO 615, Miltenyi), CD335 (PE, Beckman Coulter), CD3 (Krome-Orange, Beckman Coulter), CD56 (APC-Alexa Flour 700, Beckman Coulter), CD94 (Pacific blue, Beckman Coulter), CD19 (APC-Alexa Flour 750, Beckman Coulter), CD158b1/b2 (PC7, Beckman Coulter) and CD158a,h (PC5.5, Beckman Coulter). .. Samples were run on a Beckman Coulter Navios EX Flow Cytometer.
Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis.
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with antirabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with anti-rabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Bioprocessing:Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis.
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with antirabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with anti-rabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Expressing:Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis.
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with antirabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with anti-rabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Incubation:Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis.
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with antirabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
Article Title: Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis
Article Snippet: .. Conjugated CD3 (#552127), PD-1 (#565935) (BD Pharmingen, USA), and CD314 (anti-NKG2D) (#130–111-846), CD8 (#130–110-679), CD56 (#130–113-310) (Miltenyi Biotec, Germany) monoclonal antibodies (mAbs) were used to characterize phenotype of MSLN-CAR.CIK, and a conjugated anti-F(ab′)2 fragment Ab (#115–606-072, Jackson ImmunoResearch Europe Ltd) to detect CAR expression. mOC cells were stained with conjugated mAbs for the expression of MIC A/B (#558352, BD Pharmingen, USA) and ULBPs-2,5,6 (#FAB1298A, R&D Systems Inc., USA). mOC was stained with 1 μg/mL MSLN-Ab (#PA5-79697, Sigma-Aldrich, USA), then washed and incubated with anti-rabbit IgG-AlexaFluor 647 Secondary Ab (#A31573, Invitrogen, ThermoFisher, USA). .. Labeled cells were read on FACS Cyan ADP (Beckman Coulter, USA), analyzed using Summit Software (Beckman Coulter, USA), and on BDFACS Celesta (BD Life Sciences, USA).
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