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cd25 pe  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology cd25 pe
    Cd25 Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/PE+Anti-Mouse+CD25+Antibody/pm42909153-503-25-27
    Average 93 stars, based on 17 article reviews
    cd25 pe - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Cinobufotalin ameliorates ulcerative colitis by modulating gut microbiota, restoring Th17/Treg balance, and inhibiting MAPK/NF-κB signaling pathways.
    Article Snippet: Ethnopharmacological relevance: Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by mucosal inflammation, with symptoms including abdominal pain and bloody stools.. The pathogenesis involves immune dysregulation, gut microbiota imbalance, and environmental factors.. Current treatments have limitations, prompting the exploration of alternative therapies like traditional Chinese medicine.

    Flow Cytometry:

    Article Title: Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation
    Article Snippet: .. The antibodies used in the flow cytometry were as follows: CD4 (FITC, E-AB-F1097C), CD8a (PE, E-AB-F1104D), CD25 (PE-Cy5, E-AB-F1102G), CD69 (PE-Cy7, E-AB-F1187H), IL4 (PE, E-AB-F1204UD), IL17A (APC, E-AB-F1272E) were purchased from Elabscience Biotechnology (Wuhan, China), antibodies IFNγ (APC-Cy7, cat# 561479) was purchased from BD Biosciences (UK), and Granzyme B (PE-Cy7, cat# 372213) was purchased from Biolegend (San Diego, CA, USA). .. SLC7A5 antagonist LAT1-IN1 (HY-108540) was purchased from MedChemExpress (NJ, USA).

    Article Title: Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation.
    Article Snippet: .. The antibodies used in the flow cytometry were as follows: CD4 (FITC, E-AB-F1097C), CD8a (PE, E-AB-F1104D), CD25 (PECy5, E-AB-F1102G), CD69 (PE-Cy7, E-AB-F1187H), IL4 (PE, E-AB-F1204UD), IL17A (APC, E-AB-F1272E) were purchased from Elabscience Biotechnology (Wuhan, China), antibodies IFNg (APC-Cy7, cat# 561479) was purchased from BD Biosciences (UK), and Granzyme B (PE-Cy7, cat# 372213) was purchased from Biolegend (San Diego, CA, USA). .. SLC7A5 antagonist LAT1-IN1 (HY-108540) was purchased from MedChemExpress (NJ, USA).

    Single Cell:

    Article Title: A biomimetic solution, albumin-doxorubicin molecular complex, targeting tumor and tumor-draining lymph nodes.
    Article Snippet: Chemotherapy-induced immunologic cell death is haunted by the non-specific distribution of chemotherapeutic drugs and insignificant immune activation effects, which render efforts to inhibit the distant metastasis of tumors frustrated.. Given the pivotal role that lymph nodes play in tumor metastasis, it is of vital importance whether the drug delivery to tumor-draining lymph nodes (TDLNs) succeeds.. In the current study, we developed a doxorubicin–albumin complex (DOX–HSA) solution with the specific ability to simultaneously target the primary tumor and the TDLNs.

    Isolation:

    Article Title: A biomimetic solution, albumin-doxorubicin molecular complex, targeting tumor and tumor-draining lymph nodes.
    Article Snippet: Chemotherapy-induced immunologic cell death is haunted by the non-specific distribution of chemotherapeutic drugs and insignificant immune activation effects, which render efforts to inhibit the distant metastasis of tumors frustrated.. Given the pivotal role that lymph nodes play in tumor metastasis, it is of vital importance whether the drug delivery to tumor-draining lymph nodes (TDLNs) succeeds.. In the current study, we developed a doxorubicin–albumin complex (DOX–HSA) solution with the specific ability to simultaneously target the primary tumor and the TDLNs.

    Concentration Assay:

    Article Title: Effect of EVT-Derived Small Extracellular Vesicles on Normal and Impaired Human Implantation
    Article Snippet: .. In brief, cells adjusted to a concentration of 1 × 106 per sample were subjected to immunofluorescent staining with the following monoclonal antibodies in appropriate combinations: CD4 (Ref.: H10-041-02-H32, clone: OKT-4, SungenBiotech, Beijing, China), CD8 (Ref.: H10 081-09-G, clone: HIT8a, SungenBiotech, Beijing, China), CD69 (Ref.: 555531, clone: FN50, BD Pharmingen, San Jose, CA, USA), CD25 (Ref.: E-AB-F1194E, clone: BC96, Elabscience), HLA-DR (Ref.: 559866, clone: G46-6, BD Pharmingen), CD27 (Ref.: 48-0279-42, clone: 0323, Invitrogen Ebioscience, San Diego, USA), CD28 (Ref.: 25-0289-42, clone: CD28.2, Invitrogen Ebioscience), IFNγ (Ref.: E-AB-F1196E, clone: B27, Elabscience, San Diego, USA), TNFα (Ref.: 559321, clone: Mab11, BD Pharmingen, San Jose, CA, USA), Granzyme B (Ref.: 560211, clone: GB11, BD Pharmingen, San Jose, USA), and Perforin (Ref.: 51-65994X, clone: deltaG9, BD Pharmingen, San Jose, USA). ..

    Staining:

    Article Title: Effect of EVT-Derived Small Extracellular Vesicles on Normal and Impaired Human Implantation
    Article Snippet: .. In brief, cells adjusted to a concentration of 1 × 106 per sample were subjected to immunofluorescent staining with the following monoclonal antibodies in appropriate combinations: CD4 (Ref.: H10-041-02-H32, clone: OKT-4, SungenBiotech, Beijing, China), CD8 (Ref.: H10 081-09-G, clone: HIT8a, SungenBiotech, Beijing, China), CD69 (Ref.: 555531, clone: FN50, BD Pharmingen, San Jose, CA, USA), CD25 (Ref.: E-AB-F1194E, clone: BC96, Elabscience), HLA-DR (Ref.: 559866, clone: G46-6, BD Pharmingen), CD27 (Ref.: 48-0279-42, clone: 0323, Invitrogen Ebioscience, San Diego, USA), CD28 (Ref.: 25-0289-42, clone: CD28.2, Invitrogen Ebioscience), IFNγ (Ref.: E-AB-F1196E, clone: B27, Elabscience, San Diego, USA), TNFα (Ref.: 559321, clone: Mab11, BD Pharmingen, San Jose, CA, USA), Granzyme B (Ref.: 560211, clone: GB11, BD Pharmingen, San Jose, USA), and Perforin (Ref.: 51-65994X, clone: deltaG9, BD Pharmingen, San Jose, USA). ..

    Article Title: AML cell-derived exosomes suppress the activation and cytotoxicity of NK cells in AML via PD-1/PD-L1 pathway.
    Article Snippet: Funding information Fundamental Research Funds for the Central Universities of China, Grant/Award Numbers: DUT15RC(3)051, DUT17ZD214, DUT21YG101 Abstract Exosomes are bilayer lipid bodies and contain a variety of bioactive molecules such as proteins, lipids, and nucleic acids, and so forth.. Exosomes derived from solid tumors may play critical roles in tumor development and immune evasion.. However, the underlying effects of tumor‐derived exosomes on immune function in modulating intercellular crosstalk within the bone marrow niche during acute myeloid leukemia (AML) development and immune evasion remain largely elusive.

    Bioprocessing:

    Article Title: Effect of EVT-Derived Small Extracellular Vesicles on Normal and Impaired Human Implantation
    Article Snippet: .. In brief, cells adjusted to a concentration of 1 × 106 per sample were subjected to immunofluorescent staining with the following monoclonal antibodies in appropriate combinations: CD4 (Ref.: H10-041-02-H32, clone: OKT-4, SungenBiotech, Beijing, China), CD8 (Ref.: H10 081-09-G, clone: HIT8a, SungenBiotech, Beijing, China), CD69 (Ref.: 555531, clone: FN50, BD Pharmingen, San Jose, CA, USA), CD25 (Ref.: E-AB-F1194E, clone: BC96, Elabscience), HLA-DR (Ref.: 559866, clone: G46-6, BD Pharmingen), CD27 (Ref.: 48-0279-42, clone: 0323, Invitrogen Ebioscience, San Diego, USA), CD28 (Ref.: 25-0289-42, clone: CD28.2, Invitrogen Ebioscience), IFNγ (Ref.: E-AB-F1196E, clone: B27, Elabscience, San Diego, USA), TNFα (Ref.: 559321, clone: Mab11, BD Pharmingen, San Jose, CA, USA), Granzyme B (Ref.: 560211, clone: GB11, BD Pharmingen, San Jose, USA), and Perforin (Ref.: 51-65994X, clone: deltaG9, BD Pharmingen, San Jose, USA). ..

    Incubation:

    Article Title: AML cell-derived exosomes suppress the activation and cytotoxicity of NK cells in AML via PD-1/PD-L1 pathway.
    Article Snippet: Funding information Fundamental Research Funds for the Central Universities of China, Grant/Award Numbers: DUT15RC(3)051, DUT17ZD214, DUT21YG101 Abstract Exosomes are bilayer lipid bodies and contain a variety of bioactive molecules such as proteins, lipids, and nucleic acids, and so forth.. Exosomes derived from solid tumors may play critical roles in tumor development and immune evasion.. However, the underlying effects of tumor‐derived exosomes on immune function in modulating intercellular crosstalk within the bone marrow niche during acute myeloid leukemia (AML) development and immune evasion remain largely elusive.



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    Miltenyi Biotec human cd4 cd25 regulatory t cell isolation kit
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
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    Image Search Results


    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Control, Lysis, Flow Cytometry, Staining, In Vitro, Isolation, Incubation, Expressing, Comparison

    PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Analysis, Activation Assay, Immunohistochemistry, Expressing

    PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Tissue, Microinjection, Injection, Staining, Analysis, Expressing, Activity Assay