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cd206 apc  (Multi Sciences (Lianke) Biotech Co Ltd)
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Cd206 Apc, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of <t>CD206</t> + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.
Cd206 Apc, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of <t>CD206</t> + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.
Cd206, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of <t>CD206</t> + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.
Cd206 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of <t>CD206</t> + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.
Bioss Bs 10005r Cy5 Cd206 Apc, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of <t>CD206</t> + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of <t>CD206</t> + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.
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IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of CD206 + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.

Journal: Bioactive Materials

Article Title: Bifunctional adECM bioscaffold with STIM1-ASCs and IGF-2 promotes functional masseter VML repair via myogenesis and fibrosis suppression

doi: 10.1016/j.bioactmat.2025.08.019

Figure Lengend Snippet: IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of CD206 + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.

Article Snippet: After 72 h of co-culture, the collected cells were incubated with CD80-PE antibody (1:200, 12-0800-82, eBioscience), CD206-APC (MRC1/APC) antibody (1:100, bs-4727R, Bioss), and F4/80-FITC antibody (1:200, AER-051-F, ThermoFisher) for 30 min.

Techniques: Co-Culture Assay, Expressing, Marker, Flow Cytometry, Control, Quantitative RT-PCR