Journal: Antioxidants

Article Title: Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery

doi: 10.3390/antiox12030714

Figure Lengend Snippet: All primary and secondary antibodies used in this study.

Article Snippet: Mouse MMR/CD206 APC-conjugated Antibody , Goat , , 1:20 , R & D Systems , FAB2535A.

Techniques:

Journal: Chemical Engineering Journal

Article Title: Cryogenically 3D printed biomimetic scaffolds containing decellularized small intestinal submucosa and Sr2+/Fe3+ co-substituted hydroxyapatite for bone tissue engineering

doi: 10.1016/j.cej.2021.133459

Figure Lengend Snippet: Fig. 5. In vitro RAW cells polarization modulation after cultured on scaffolds for 3 days. (A) Calcein-AM staining of RAW; (B) RAW cells polarization examined by double marked immunofluorescence of NOS2/Arg-1; (C) RAW polarization quantified by double marked flow cytometry of CD86/CD206; (D) Quantitative CD206 and CD86 expression ratios; (E) RT-qPCR analysis of relative IL6, IL1-β, TNF-α, Arg-1 and IL10 gene expressions; (F) RT-qPCR analysis of relative VEGF, PDGF-BB, TGF-β1 and BMP2 gene expressions. Data represent the mean ± SD. *p < 0.05 versus SIS; #p < 0.05 versus SIS/FeHA.

Article Snippet: Briefly, RAW cells were harvested and incubated with CD86 antibody (553692, BD Pharmingen, USA) under 4 °C for 30 min. Then, cells were washed, resuspended, and incubated with CD206 antibody (FAB25351A, R&D Systems, USA) again to be analyzed using the flow cytometer (Beckman Coulter, CytoFLEX).

Techniques: In Vitro, Cell Culture, Staining, Immunofluorescence, Flow Cytometry, Expressing, Quantitative RT-PCR

Journal: Chemical Engineering Journal

Article Title: Cryogenically 3D printed biomimetic scaffolds containing decellularized small intestinal submucosa and Sr2+/Fe3+ co-substituted hydroxyapatite for bone tissue engineering

doi: 10.1016/j.cej.2021.133459

Figure Lengend Snippet: Fig. 7. In vivo immunomodulation and vascularization of scaffolds following 7 days subcutaneous implantation. (A) The histological graphs of implanted scaffolds : from the general view of horizontal HE sections to internal sites stained by HE, NOS2, CD206, and CD31 IHC in similar positions; (B). Quantitation of CD206 and NOS2 positive stained areas; (C) Quantitative CD206/NOS2 ratios; (D) Quantitation of new vessels density. Red arrows indicate neo-vessels stained by CD31. Data represent the mean ± SD. *p < 0.05 versus SIS; &p < 0.05 versus SIS/FeHA; #p < 0.05 versus SIS/SrHA.

Article Snippet: Briefly, RAW cells were harvested and incubated with CD86 antibody (553692, BD Pharmingen, USA) under 4 °C for 30 min. Then, cells were washed, resuspended, and incubated with CD206 antibody (FAB25351A, R&D Systems, USA) again to be analyzed using the flow cytometer (Beckman Coulter, CytoFLEX).

Techniques: In Vivo, Staining, Quantitation Assay

Journal: Biomaterials Research

Article Title: Custom-Made Ce–Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization

doi: 10.34133/bmr.0118

Figure Lengend Snippet: CeMn-PEG could promote the polarization of macrophages to M2 type. (A to C) Representative images and relative fluorescence intensity of CD86 and CD206 in RAW264.7 cells in the coculture system. (D and E) Representative images of cytometry analysis (D) and mean fluorescence intensity (MFI) quantification (E) of CD86 and CD206 in RAW264.7 cells in the coculture system. (F and G) Representative images and quantification data of Western blot results of IL-1β, IL-4, IL-6, and IL-10 in RAW264.7 cells in the coculture system. Scale bar, 25 μm. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3.

Article Snippet: After NP and RAW264.7 cells were treated as previously described, the digested single-cell suspension was incubated with flow cytometry antibodies, CD86-FITC (fluorescein isothiocyanate), and CD206-APC (allophycocyanin) at room temperature in the dark for 1 h. The V-APC/7-AAD Apoptosis Detection Kit (KeyGEN) was used according to the manufacturer’s instruction.

Techniques: Fluorescence, Cytometry, Western Blot