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cd154 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd154 antibody, anti-human, reafinity
    Cd154 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd154/CD154+Antibody%2C+anti-human%2C+REAfinity/custom%40130-114-138%4042447860
    Average 94 stars, based on 44 article reviews
    cd154 antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment
    Article Snippet: After 5 h of incubation at 5% CO 2 and 37 °C, 1 μg/ml Brefeldin A (Sigma-Aldrich, B6542) was added to inhibit the secretion of activation markers. .. After another 2 h, antigen-specific T H cells were isolated using CD154-dependent magnetic activated cell sorting (MACS®) with the anti-CD154 Microbead Kit human (Miltenyi, 130–092–658) and MS columns (Miltenyi, 130–042–201). ..

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment.
    Article Snippet: After 5 h of incubation at 5% CO2 and 37 °C, 1 μg/ml Brefeldin A (Sigma-Aldrich, B6542) was added to inhibit the secretion of activation markers. .. After another 2 h, antigen-specific TH cells were isolated using CD154-dependent magnetic activated cell sorting (MACS®) with the anti-CD154 Microbead Kit human (Miltenyi, 130–092–658) and MS columns (Miltenyi, 130–042–201). ..

    FACS:

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment
    Article Snippet: After 5 h of incubation at 5% CO 2 and 37 °C, 1 μg/ml Brefeldin A (Sigma-Aldrich, B6542) was added to inhibit the secretion of activation markers. .. After another 2 h, antigen-specific T H cells were isolated using CD154-dependent magnetic activated cell sorting (MACS®) with the anti-CD154 Microbead Kit human (Miltenyi, 130–092–658) and MS columns (Miltenyi, 130–042–201). ..

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment.
    Article Snippet: After 5 h of incubation at 5% CO2 and 37 °C, 1 μg/ml Brefeldin A (Sigma-Aldrich, B6542) was added to inhibit the secretion of activation markers. .. After another 2 h, antigen-specific TH cells were isolated using CD154-dependent magnetic activated cell sorting (MACS®) with the anti-CD154 Microbead Kit human (Miltenyi, 130–092–658) and MS columns (Miltenyi, 130–042–201). ..

    Magnetic Cell Separation:

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment
    Article Snippet: After 5 h of incubation at 5% CO 2 and 37 °C, 1 μg/ml Brefeldin A (Sigma-Aldrich, B6542) was added to inhibit the secretion of activation markers. .. After another 2 h, antigen-specific T H cells were isolated using CD154-dependent magnetic activated cell sorting (MACS®) with the anti-CD154 Microbead Kit human (Miltenyi, 130–092–658) and MS columns (Miltenyi, 130–042–201). ..

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment.
    Article Snippet: After 5 h of incubation at 5% CO2 and 37 °C, 1 μg/ml Brefeldin A (Sigma-Aldrich, B6542) was added to inhibit the secretion of activation markers. .. After another 2 h, antigen-specific TH cells were isolated using CD154-dependent magnetic activated cell sorting (MACS®) with the anti-CD154 Microbead Kit human (Miltenyi, 130–092–658) and MS columns (Miltenyi, 130–042–201). ..

    Staining:

    Article Title: Ageing and inflammation limit the induction of SARS-CoV-2-specific CD8+ T cell responses in severe COVID-19.
    Article Snippet: Nonviable events were excluded using a LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific). .. Cells were then fixed/permeabilized using an Inside Stain Kit (Miltenyi Biotec) and stained with directly conjugated antibodies specific for CD154 (clone REA238, Miltenyi Biotec), IFN-γ (clone REA600, Miltenyi Biotec), TNF (clone MAb11, BioLegend), and IL-2 (clone REA689, Miltenyi Biotec). .. Cells from patients in the secondary cohort were washed and stained with directly conjugated monoclonal antibodies specific for CD3 (clone SP34-2, BD Biosciences), CD4 (clone L200, BD Biosciences), CD8 (clone RPAT8, BD Biosciences), CD27 (clone 1A4CD27, Beckman Coulter), and CD45RO (clone UCHL1, BD Biosciences).

    Article Title: NK cells shape the clonal evolution of B-ALL cells by IFN-γ production
    Article Snippet: .. The following antibodies (clones) were used for the staining: CD48 (REA1238), CD112 (829038), CD137L (REA962), CD154 (REA785), CD155 (REA519), TRAIL-R (CD261) (REA1233), Qa-1b (6A8.6F10.1A6), Qa-2a (REA523), H-2Db (REA1198), H-2Kb (REA619), Rae1 (REA723) and Ly6a/Sca-1 (REA422) purchased from Miltenyi Biotec. ..

    Article Title: Aging and inflammation limit the induction of SARS-CoV-2–specific CD8 + T cell responses in severe COVID-19
    Article Snippet: Nonviable events were excluded using a LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific). .. Cells were then fixed/permeabilized using an Inside Stain Kit (Miltenyi Biotec) and stained with directly conjugated antibodies specific for CD154 (clone REA238, Miltenyi Biotec), IFN-γ (clone REA600, Miltenyi Biotec), TNF (clone MAb11, BioLegend), and IL-2 (clone REA689, Miltenyi Biotec). .. Cells from patients in the secondary cohort were washed and stained with directly conjugated monoclonal antibodies specific for CD3 (clone SP34-2, BD Biosciences), CD4 (clone L200, BD Biosciences), CD8 (clone RPA-T8, BD Biosciences), CD27 (clone 1A4CD27, Beckman Coulter), and CD45RO (clone UCHL1, BD Biosciences).

    Article Title: Rapid protection induced by a single-shot Lassa vaccine in male cynomolgus monkeys
    Article Snippet: Samples were then treated with PBS-EDTA before staining with CD3 CD3 (557597, APC mouse anti-human, clone SP34-2, 14 μL), CD4 (560836, Alexa Fluor R 700 mouse anti-human, clone L200, 5 μL), and CD8 (560179, APC-H7 mouse anti-human, clone SK1, 5 μL) antibodies from BD biosciences. .. After fixation and permeabilization, cells were stained with IFNγ (559327, PE mouse anti-human, clone B27, 20 μL) and TNFα (557647, PE-Cy TM 7 mouse anti-human, clone Mab11, 5 μL) antibodies from BD Biosciences, CD137 (130-119-886, VioBright FITC mouse anti-human, clone 4B4-1, 2 μL), and CD154 (130-113-609, VioBlue mouse anti-human, clone 5C8, 2 μL) antibodies from Miltenyi Biotec. .. Cells were analyzed by flow cytometry using a 10-color Gallios cytometer (Beckman Coulter) and the collected data analyzed using Kaluza v.2.1 software Beckman Coulter).



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