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cd141  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd141
    Cd141, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd141/CD141+(BDCA-3)+MicroBead+Kit%2C+human/10__1080_slash_2162402x__2026__2695692-57-7-9
    Average 91 stars, based on 10 article reviews
    cd141 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Selection:

    Article Title: Intratumoral administration of the immunologic adjuvant AS01 B in combination with autologous CD1c (BDCA-1) + /CD141 (BDCA-3) + myeloid dendritic cells plus ipilimumab and intravenous nivolumab in patients with refractory advanced melanoma.
    Article Snippet: .. First, CD14+ and CD19+ cells were depleted with the CliniMACS CD14 and CD19 reagents followed by a positive selection of CD1c (BDCA- 1)+ myDC and CD141 (BDCA- 3)+ myDC by using CliniMACS CD1c (BDCA- 1)- biotin microbeads, CliniMACS CD141 (BDCA- 3)- biotin microbeads and CliniMACS Anti- Biotin Reagent (Miltenyi) using the CliniMACS Prodigy platform (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL).

    other:

    Article Title: Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC
    Article Snippet: CD141 , APC , AD5‐14H12 , Miltenyi , 130‐113‐314 , 1:125.

    Article Title: Ex vivo-generated conventional dendritic cells type 1 and type 2 from blood progenitors induce potent antigen-specific T-cell immunity
    Article Snippet: Alternatively, DCs were enriched by MACS using CD141-Microbeads (130-090-512, Miltenyi Biotec) and CD1c Isolation kit (130-119-475, Miltenyi Biotec) sequentially (see supplementary figure 1C).

    Incubation:

    Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia
    Article Snippet: VMSCs and ADSCs were blocked with a human FcR blocking reagent (Miltenyi Biotec), then centrifuged at 500 g at 4 °C for 5 min. .. The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec). ..

    Article Title: Decreased levels and function of dendritic cells in blood and airways predict COVID ‐19 severity
    Article Snippet: .. After Live/Dead Blue staining (Invitrogen), cells were incubated with human FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) and then stained with antibodies against surface proteins: CD1c (AD5‐8E7; Miltenyi Biotec), CD3 (SK7; Becton, Dickinson and Company (BD), Franklin Lakes, NJ, USA), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3GE; BioLegend, San Diego, CA, USA), CD19 (HIB19; BioLegend), CD20 (L27; BD), CD45 (HI30; BD), CD56 (HCD56; BD), CD62L (SK11; BD), CD66abce (TET2; Miltenyi Biotec), CD86 (2331; BD), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi Biotec), CCR2 (K036C2; BioLegend), CCR7 (150 503; BD) and HLA‐DR (TU36; Life Technologies, Waltham, MA, USA). .. A subset of respiratory samples were stained with another panel: CD3 (SP34‐2; BBD), CD4 (L200; BD), CD11c (B‐ly6; BD), CD14 (M5E2; BD), CD16 (3G8; BD), CD19 (SJ25‐C1; Thermo Fisher Scientific, Waltham, MA, USA), CD45 (HI30; BD), CD56 (HCD56; BioLegend), CD66abce (TET2; Miltenyi Biotec), CD123 (7G3; BD), LOX‐1 (15C4; BioLegend) and HLA‐DR (L243; BioLegend).

    Article Title: Decreased levels and function of dendritic cells in blood and airways predict COVID ‐19 severity
    Article Snippet: .. Cells were washed with PBS and stained using Live/Dead Blue (Invitrogen, Thermo Fisher Scientific), incubated with human FcR blocking reagent (Miltenyi Biotec), and stained at 4°C for 20 min with antibodies against the following surface proteins: CD1c (AD5‐8E7; Miltenyi Biotec), CD3 (SK7; BD), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3GE; BioLegend), CD19 (HIB19; BioLegend), CD20 (L27; BD), CD45 (HI30; BD), CD56 (HCD56; BD), CD66abce (TET2; Miltenyi Biotec), CD86 (2331; BD), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi Biotec), and HLA‐DR (TU36; Life Technologies, Thermo Fisher Scientific). .. Cells were fixed and permeabilised with permeabilisation buffer (Thermo Fisher Scientific) and stained with antibodies against IFN‐α (LT27:295, Miltenyi Biotec), IL‐6 (MQ2‐13A5, Thermo Fisher Scientific), and TNF (Mab11, BD) at 4°C for 15 min.

    Isolation:

    Article Title: Intratumoral administration of the immunologic adjuvant AS01 B in combination with autologous CD1c (BDCA-1) + /CD141 (BDCA-3) + myeloid dendritic cells plus ipilimumab and intravenous nivolumab in patients with refractory advanced melanoma.
    Article Snippet: First, CD14+ and CD19+ cells were depleted with the CliniMACS CD14 and CD19 reagents followed by a positive selection of CD1c (BDCA- 1)+ myDC and CD141 (BDCA- 3)+ myDC by using CliniMACS CD1c (BDCA- 1)- biotin microbeads, CliniMACS CD141 (BDCA- 3)- biotin microbeads and CliniMACS Anti- Biotin Reagent (Miltenyi) using the CliniMACS Prodigy platform (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL).

    Centrifugation:

    Article Title: Intratumoral administration of the immunologic adjuvant AS01 B in combination with autologous CD1c (BDCA-1) + /CD141 (BDCA-3) + myeloid dendritic cells plus ipilimumab and intravenous nivolumab in patients with refractory advanced melanoma.
    Article Snippet: First, CD14+ and CD19+ cells were depleted with the CliniMACS CD14 and CD19 reagents followed by a positive selection of CD1c (BDCA- 1)+ myDC and CD141 (BDCA- 3)+ myDC by using CliniMACS CD1c (BDCA- 1)- biotin microbeads, CliniMACS CD141 (BDCA- 3)- biotin microbeads and CliniMACS Anti- Biotin Reagent (Miltenyi) using the CliniMACS Prodigy platform (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL).

    Saline:

    Article Title: Intratumoral administration of the immunologic adjuvant AS01 B in combination with autologous CD1c (BDCA-1) + /CD141 (BDCA-3) + myeloid dendritic cells plus ipilimumab and intravenous nivolumab in patients with refractory advanced melanoma.
    Article Snippet: First, CD14+ and CD19+ cells were depleted with the CliniMACS CD14 and CD19 reagents followed by a positive selection of CD1c (BDCA- 1)+ myDC and CD141 (BDCA- 3)+ myDC by using CliniMACS CD1c (BDCA- 1)- biotin microbeads, CliniMACS CD141 (BDCA- 3)- biotin microbeads and CliniMACS Anti- Biotin Reagent (Miltenyi) using the CliniMACS Prodigy platform (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL).

    Suspension:

    Article Title: Intratumoral administration of the immunologic adjuvant AS01 B in combination with autologous CD1c (BDCA-1) + /CD141 (BDCA-3) + myeloid dendritic cells plus ipilimumab and intravenous nivolumab in patients with refractory advanced melanoma.
    Article Snippet: First, CD14+ and CD19+ cells were depleted with the CliniMACS CD14 and CD19 reagents followed by a positive selection of CD1c (BDCA- 1)+ myDC and CD141 (BDCA- 3)+ myDC by using CliniMACS CD1c (BDCA- 1)- biotin microbeads, CliniMACS CD141 (BDCA- 3)- biotin microbeads and CliniMACS Anti- Biotin Reagent (Miltenyi) using the CliniMACS Prodigy platform (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL). .. The isolated CD1c (BDCA- 1)+ and CD141 (BDCA- 3)+ myDC fraction was concentrated by centrifugation and resuspended in phosphate- buffered saline/EDTA (Miltenyi) containing 0.5% human albumin to obtain a cell suspension at volume desired for clinical administration (according to tumor diameter, up to maximum 4 mL).

    Staining:

    Article Title: Decreased levels and function of dendritic cells in blood and airways predict COVID ‐19 severity
    Article Snippet: .. After Live/Dead Blue staining (Invitrogen), cells were incubated with human FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) and then stained with antibodies against surface proteins: CD1c (AD5‐8E7; Miltenyi Biotec), CD3 (SK7; Becton, Dickinson and Company (BD), Franklin Lakes, NJ, USA), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3GE; BioLegend, San Diego, CA, USA), CD19 (HIB19; BioLegend), CD20 (L27; BD), CD45 (HI30; BD), CD56 (HCD56; BD), CD62L (SK11; BD), CD66abce (TET2; Miltenyi Biotec), CD86 (2331; BD), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi Biotec), CCR2 (K036C2; BioLegend), CCR7 (150 503; BD) and HLA‐DR (TU36; Life Technologies, Waltham, MA, USA). .. A subset of respiratory samples were stained with another panel: CD3 (SP34‐2; BBD), CD4 (L200; BD), CD11c (B‐ly6; BD), CD14 (M5E2; BD), CD16 (3G8; BD), CD19 (SJ25‐C1; Thermo Fisher Scientific, Waltham, MA, USA), CD45 (HI30; BD), CD56 (HCD56; BioLegend), CD66abce (TET2; Miltenyi Biotec), CD123 (7G3; BD), LOX‐1 (15C4; BioLegend) and HLA‐DR (L243; BioLegend).

    Article Title: Decreased levels and function of dendritic cells in blood and airways predict COVID ‐19 severity
    Article Snippet: .. Cells were washed with PBS and stained using Live/Dead Blue (Invitrogen, Thermo Fisher Scientific), incubated with human FcR blocking reagent (Miltenyi Biotec), and stained at 4°C for 20 min with antibodies against the following surface proteins: CD1c (AD5‐8E7; Miltenyi Biotec), CD3 (SK7; BD), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3GE; BioLegend), CD19 (HIB19; BioLegend), CD20 (L27; BD), CD45 (HI30; BD), CD56 (HCD56; BD), CD66abce (TET2; Miltenyi Biotec), CD86 (2331; BD), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi Biotec), and HLA‐DR (TU36; Life Technologies, Thermo Fisher Scientific). .. Cells were fixed and permeabilised with permeabilisation buffer (Thermo Fisher Scientific) and stained with antibodies against IFN‐α (LT27:295, Miltenyi Biotec), IL‐6 (MQ2‐13A5, Thermo Fisher Scientific), and TNF (Mab11, BD) at 4°C for 15 min.

    Blocking Assay:

    Article Title: Decreased levels and function of dendritic cells in blood and airways predict COVID ‐19 severity
    Article Snippet: .. After Live/Dead Blue staining (Invitrogen), cells were incubated with human FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) and then stained with antibodies against surface proteins: CD1c (AD5‐8E7; Miltenyi Biotec), CD3 (SK7; Becton, Dickinson and Company (BD), Franklin Lakes, NJ, USA), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3GE; BioLegend, San Diego, CA, USA), CD19 (HIB19; BioLegend), CD20 (L27; BD), CD45 (HI30; BD), CD56 (HCD56; BD), CD62L (SK11; BD), CD66abce (TET2; Miltenyi Biotec), CD86 (2331; BD), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi Biotec), CCR2 (K036C2; BioLegend), CCR7 (150 503; BD) and HLA‐DR (TU36; Life Technologies, Waltham, MA, USA). .. A subset of respiratory samples were stained with another panel: CD3 (SP34‐2; BBD), CD4 (L200; BD), CD11c (B‐ly6; BD), CD14 (M5E2; BD), CD16 (3G8; BD), CD19 (SJ25‐C1; Thermo Fisher Scientific, Waltham, MA, USA), CD45 (HI30; BD), CD56 (HCD56; BioLegend), CD66abce (TET2; Miltenyi Biotec), CD123 (7G3; BD), LOX‐1 (15C4; BioLegend) and HLA‐DR (L243; BioLegend).

    Article Title: Decreased levels and function of dendritic cells in blood and airways predict COVID ‐19 severity
    Article Snippet: .. Cells were washed with PBS and stained using Live/Dead Blue (Invitrogen, Thermo Fisher Scientific), incubated with human FcR blocking reagent (Miltenyi Biotec), and stained at 4°C for 20 min with antibodies against the following surface proteins: CD1c (AD5‐8E7; Miltenyi Biotec), CD3 (SK7; BD), CD11c (B‐Ly6; BD), CD14 (M5E2; BD), CD16 (3GE; BioLegend), CD19 (HIB19; BioLegend), CD20 (L27; BD), CD45 (HI30; BD), CD56 (HCD56; BD), CD66abce (TET2; Miltenyi Biotec), CD86 (2331; BD), CD123 (7G3; BD), CD141 (AD5‐14H12; Miltenyi Biotec), and HLA‐DR (TU36; Life Technologies, Thermo Fisher Scientific). .. Cells were fixed and permeabilised with permeabilisation buffer (Thermo Fisher Scientific) and stained with antibodies against IFN‐α (LT27:295, Miltenyi Biotec), IL‐6 (MQ2‐13A5, Thermo Fisher Scientific), and TNF (Mab11, BD) at 4°C for 15 min.



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