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247emt  (ATCC)


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    ATCC 247emt
    247emt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl+247emt/HCT116+VIM+RFP/pmc12723371-23-4-2
    Average 99 stars, based on 475 article reviews
    247emt - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: A cancer-specific antigen drives histone acetylation by stabilizing the acetyltransferases.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 Effectene Transfection Reagent QIAGEN Cat# 301427 Proteinase inhibitor cocktail NCM Cat# P001 Pierce Protein A/G Magnetic Beads Thermo Scientifc Cat# 88802 Pierce IP lysis buffer Thermo Scientifc Cat# 87787 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 DAPI Abcam Cat# ab104139 Matrigel Corning Cat# 354234 Crystal violet Beyotime Cat# C0121 Recombinant Human His6-Ubiquitin E1 Enzyme R&D system Cat# E− 304-050 Recombinant Human UbcH5a/UBE2D1 Protein R&D system Cat# E2-616-100 Recombinant Human CUL1/RBX1 Neddylated Complex Protei R&D system Cat# E3-411-025 MgATP Solution R&D system Cat# B-20 Recombinant Human Ubiquitin Protein R&D system Cat# U-100H-10M Critical commercial assays TUBE LifeSensors Cat# UM502M K48 TUBE LifeSensors Cat# UM-0407M K63 TUBE LifeSensors Cat# UM-0404M Cell Counting Kit-8 MedChemExpress Cat# HY-K0301 ECL Enhanced Plus Kit Abclonal Cat# RM00021P Pierce BCA Protein Assay Kit Thermo Scientifc Cat# 23225 Deposited data Mass spectrometry raw data ProteomeXchange Consortium via the iProX partner repository PXD067966 Experimental models: Cell lines A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 SK-MEL-2 ATCC Cat# HTB-68; RRID: CVCL_0069 HCT116 ATCC Cat# CCL-247EMT; RRID: CVCL_0291 HeLa ATCC Cat# CRM-CCL-2; RRID: CVCL_0030 H446 ATCC Cat# HTB-171; RRID: CVCL_1562 H460 ATCC Cat# HTB-177; RRID: CVCL_0459 H1975 ATCC Cat# CRL-5908; RRID: CVCL_1511 H1299 ATCC Cat# CRL-5803; RRID: CVCL_0060 H2126 ATCC Cat# CCL-256; RRID: CVCL_1532 H1650 ATCC Cat# CRL-5883; RRID: CVCL_1483 M059K ATCC Cat# CRL-2365; RRID: CVCL_0401 U2OS ATCC Cat# HTB-96; RRID: CVCL_0042 Experimental models: Organisms/strains BALB/c nude mice GemPharmatech Co., Ltd RRID: mgi:5654397 Oligonucleotides siRNA targeting NC Forward: 5’ -UUCUCCGAACGUGUCACGUTT-3′ Reverse: 5′- ACGUGACACGUUCGGAGAATT-3′ OBiO Technology N/A (Continued on next page) Cell Reports 44, 116408, October 28, 2025 19


    Virus:

    Article Title: 53BP1 regulates p53-E2F7-dependent transcriptional gene repression and participates in the Fanconi anemia pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 53BP1(WB) Cell Signaling Technology Cat# 4937; RRID:AB_10694558 53BP1(ChIP) Thermo Fisher Cat# PA1-16565; RRID:AB_568422 E2F7 Abcam Cat# ab245655; RRID:AB_3224407 p53 Cell Signaling Technology Cat# 9282S; RRID:AB_331476 β-tublin Proteintech Cat# 10068-1-AP; RRID:AB_2303998 β-actin Proteintech Cat# 20536-1-AP; RRID:AB_10700003 GFP Thermo Fisher Cat# MA5-15256; RRID:AB_10979281 FANCC Proteintech Cat# 16973-1-AP; RRID:AB_2231524 FANCI Proteintech Cat# 20789-1-AP; RRID:AB_10694829 RAD51 Proteintech Cat# 14961-1-AP; RRID:AB_2177083 H4K20me2 Abcam Cat# ab9052; RRID:AB_1951942 H3K9me3 ABclonal Cat# A22295; RRID:AB_3066640 H3K27me3 ABclonal Cat# A2771; RRID:AB_3697635 p-CHK1-345 Cell Signaling Technology Cat# 2348S; RRID:AB_331212 p-Trim28 Abcam Cat# ab133440; RRID: AB_3712532 Bacterial and virus strains E. coli DH5α Thermo Fisher Cat# 18265017 Chemicals, peptides, and recombinant proteins polybrene Sigma-Aldrich Cat# 107689 puromycin Solarbio Cat# P8230 doxycycline Sigma-Aldrich Cat# D9891 thymidine MCE Cat# HY-N115 mitomycin C MCE Cat# HY-13316 formaldehyde Sigma-Aldrich Cat# F8775 Camptothecin MCE Cat# HY-16560 Nutlin-3a MCE Cat# HY-10029 Nu7441 MCE Cat# HY-11006 RNAiso Plus Takara Cat# 9109 RNAiMAX Invitrogen Cat# 13778150 Protein A/G beads MCE Cat# HY-K0202 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R223 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PI Sigma-Aldrich Cat# P4170 Enhanced chemiluminescence (ECL) Bio-Rad Cat# 1705061 Critical commercial assays Cell Counting Kit-8 Abbkine Cat# KTA1020 Deposited data Raw and analyzed data This study GSE280049 Experimental models: Cell lines HEK-293T American Type Culture Collection (ATCC) Cat# CRL-3216 U2OS American Type Culture Collection (ATCC) Cat# HTB-96 T47D American Type Culture Collection (ATCC) Cat# HTB-133 hTERT-RPE1 American Type Culture Collection (ATCC) Cat# CRL-4000 HCT116 American Type Culture Collection (ATCC) Cat# CCL-247EMT (Continued on next page) 18 Cell Reports 44, 116152, August 26, 2025 .. Cell lines and cell culture HEK-293T, MDA-MB-231, MDA-MB-468, BT-549, U2OS, T47D, hTERT-RPE1, HCT116, HCC38 and H1299 cells were obtained from the American Type Culture Collection (ATCC), while Huh7 cells were sourced from the Japanese Collection of Research Bioresources (JCRB).

    Recombinant:

    Article Title: 53BP1 regulates p53-E2F7-dependent transcriptional gene repression and participates in the Fanconi anemia pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 53BP1(WB) Cell Signaling Technology Cat# 4937; RRID:AB_10694558 53BP1(ChIP) Thermo Fisher Cat# PA1-16565; RRID:AB_568422 E2F7 Abcam Cat# ab245655; RRID:AB_3224407 p53 Cell Signaling Technology Cat# 9282S; RRID:AB_331476 β-tublin Proteintech Cat# 10068-1-AP; RRID:AB_2303998 β-actin Proteintech Cat# 20536-1-AP; RRID:AB_10700003 GFP Thermo Fisher Cat# MA5-15256; RRID:AB_10979281 FANCC Proteintech Cat# 16973-1-AP; RRID:AB_2231524 FANCI Proteintech Cat# 20789-1-AP; RRID:AB_10694829 RAD51 Proteintech Cat# 14961-1-AP; RRID:AB_2177083 H4K20me2 Abcam Cat# ab9052; RRID:AB_1951942 H3K9me3 ABclonal Cat# A22295; RRID:AB_3066640 H3K27me3 ABclonal Cat# A2771; RRID:AB_3697635 p-CHK1-345 Cell Signaling Technology Cat# 2348S; RRID:AB_331212 p-Trim28 Abcam Cat# ab133440; RRID: AB_3712532 Bacterial and virus strains E. coli DH5α Thermo Fisher Cat# 18265017 Chemicals, peptides, and recombinant proteins polybrene Sigma-Aldrich Cat# 107689 puromycin Solarbio Cat# P8230 doxycycline Sigma-Aldrich Cat# D9891 thymidine MCE Cat# HY-N115 mitomycin C MCE Cat# HY-13316 formaldehyde Sigma-Aldrich Cat# F8775 Camptothecin MCE Cat# HY-16560 Nutlin-3a MCE Cat# HY-10029 Nu7441 MCE Cat# HY-11006 RNAiso Plus Takara Cat# 9109 RNAiMAX Invitrogen Cat# 13778150 Protein A/G beads MCE Cat# HY-K0202 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R223 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PI Sigma-Aldrich Cat# P4170 Enhanced chemiluminescence (ECL) Bio-Rad Cat# 1705061 Critical commercial assays Cell Counting Kit-8 Abbkine Cat# KTA1020 Deposited data Raw and analyzed data This study GSE280049 Experimental models: Cell lines HEK-293T American Type Culture Collection (ATCC) Cat# CRL-3216 U2OS American Type Culture Collection (ATCC) Cat# HTB-96 T47D American Type Culture Collection (ATCC) Cat# HTB-133 hTERT-RPE1 American Type Culture Collection (ATCC) Cat# CRL-4000 HCT116 American Type Culture Collection (ATCC) Cat# CCL-247EMT (Continued on next page) 18 Cell Reports 44, 116152, August 26, 2025 .. Cell lines and cell culture HEK-293T, MDA-MB-231, MDA-MB-468, BT-549, U2OS, T47D, hTERT-RPE1, HCT116, HCC38 and H1299 cells were obtained from the American Type Culture Collection (ATCC), while Huh7 cells were sourced from the Japanese Collection of Research Bioresources (JCRB).

    Real-time Polymerase Chain Reaction:

    Article Title: 53BP1 regulates p53-E2F7-dependent transcriptional gene repression and participates in the Fanconi anemia pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 53BP1(WB) Cell Signaling Technology Cat# 4937; RRID:AB_10694558 53BP1(ChIP) Thermo Fisher Cat# PA1-16565; RRID:AB_568422 E2F7 Abcam Cat# ab245655; RRID:AB_3224407 p53 Cell Signaling Technology Cat# 9282S; RRID:AB_331476 β-tublin Proteintech Cat# 10068-1-AP; RRID:AB_2303998 β-actin Proteintech Cat# 20536-1-AP; RRID:AB_10700003 GFP Thermo Fisher Cat# MA5-15256; RRID:AB_10979281 FANCC Proteintech Cat# 16973-1-AP; RRID:AB_2231524 FANCI Proteintech Cat# 20789-1-AP; RRID:AB_10694829 RAD51 Proteintech Cat# 14961-1-AP; RRID:AB_2177083 H4K20me2 Abcam Cat# ab9052; RRID:AB_1951942 H3K9me3 ABclonal Cat# A22295; RRID:AB_3066640 H3K27me3 ABclonal Cat# A2771; RRID:AB_3697635 p-CHK1-345 Cell Signaling Technology Cat# 2348S; RRID:AB_331212 p-Trim28 Abcam Cat# ab133440; RRID: AB_3712532 Bacterial and virus strains E. coli DH5α Thermo Fisher Cat# 18265017 Chemicals, peptides, and recombinant proteins polybrene Sigma-Aldrich Cat# 107689 puromycin Solarbio Cat# P8230 doxycycline Sigma-Aldrich Cat# D9891 thymidine MCE Cat# HY-N115 mitomycin C MCE Cat# HY-13316 formaldehyde Sigma-Aldrich Cat# F8775 Camptothecin MCE Cat# HY-16560 Nutlin-3a MCE Cat# HY-10029 Nu7441 MCE Cat# HY-11006 RNAiso Plus Takara Cat# 9109 RNAiMAX Invitrogen Cat# 13778150 Protein A/G beads MCE Cat# HY-K0202 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R223 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PI Sigma-Aldrich Cat# P4170 Enhanced chemiluminescence (ECL) Bio-Rad Cat# 1705061 Critical commercial assays Cell Counting Kit-8 Abbkine Cat# KTA1020 Deposited data Raw and analyzed data This study GSE280049 Experimental models: Cell lines HEK-293T American Type Culture Collection (ATCC) Cat# CRL-3216 U2OS American Type Culture Collection (ATCC) Cat# HTB-96 T47D American Type Culture Collection (ATCC) Cat# HTB-133 hTERT-RPE1 American Type Culture Collection (ATCC) Cat# CRL-4000 HCT116 American Type Culture Collection (ATCC) Cat# CCL-247EMT (Continued on next page) 18 Cell Reports 44, 116152, August 26, 2025 .. Cell lines and cell culture HEK-293T, MDA-MB-231, MDA-MB-468, BT-549, U2OS, T47D, hTERT-RPE1, HCT116, HCC38 and H1299 cells were obtained from the American Type Culture Collection (ATCC), while Huh7 cells were sourced from the Japanese Collection of Research Bioresources (JCRB).

    CCK-8 Assay:

    Article Title: 53BP1 regulates p53-E2F7-dependent transcriptional gene repression and participates in the Fanconi anemia pathway.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 53BP1(WB) Cell Signaling Technology Cat# 4937; RRID:AB_10694558 53BP1(ChIP) Thermo Fisher Cat# PA1-16565; RRID:AB_568422 E2F7 Abcam Cat# ab245655; RRID:AB_3224407 p53 Cell Signaling Technology Cat# 9282S; RRID:AB_331476 β-tublin Proteintech Cat# 10068-1-AP; RRID:AB_2303998 β-actin Proteintech Cat# 20536-1-AP; RRID:AB_10700003 GFP Thermo Fisher Cat# MA5-15256; RRID:AB_10979281 FANCC Proteintech Cat# 16973-1-AP; RRID:AB_2231524 FANCI Proteintech Cat# 20789-1-AP; RRID:AB_10694829 RAD51 Proteintech Cat# 14961-1-AP; RRID:AB_2177083 H4K20me2 Abcam Cat# ab9052; RRID:AB_1951942 H3K9me3 ABclonal Cat# A22295; RRID:AB_3066640 H3K27me3 ABclonal Cat# A2771; RRID:AB_3697635 p-CHK1-345 Cell Signaling Technology Cat# 2348S; RRID:AB_331212 p-Trim28 Abcam Cat# ab133440; RRID: AB_3712532 Bacterial and virus strains E. coli DH5α Thermo Fisher Cat# 18265017 Chemicals, peptides, and recombinant proteins polybrene Sigma-Aldrich Cat# 107689 puromycin Solarbio Cat# P8230 doxycycline Sigma-Aldrich Cat# D9891 thymidine MCE Cat# HY-N115 mitomycin C MCE Cat# HY-13316 formaldehyde Sigma-Aldrich Cat# F8775 Camptothecin MCE Cat# HY-16560 Nutlin-3a MCE Cat# HY-10029 Nu7441 MCE Cat# HY-11006 RNAiso Plus Takara Cat# 9109 RNAiMAX Invitrogen Cat# 13778150 Protein A/G beads MCE Cat# HY-K0202 HiScript II Q RT SuperMix for qPCR Vazyme Cat# R223 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PI Sigma-Aldrich Cat# P4170 Enhanced chemiluminescence (ECL) Bio-Rad Cat# 1705061 Critical commercial assays Cell Counting Kit-8 Abbkine Cat# KTA1020 Deposited data Raw and analyzed data This study GSE280049 Experimental models: Cell lines HEK-293T American Type Culture Collection (ATCC) Cat# CRL-3216 U2OS American Type Culture Collection (ATCC) Cat# HTB-96 T47D American Type Culture Collection (ATCC) Cat# HTB-133 hTERT-RPE1 American Type Culture Collection (ATCC) Cat# CRL-4000 HCT116 American Type Culture Collection (ATCC) Cat# CCL-247EMT (Continued on next page) 18 Cell Reports 44, 116152, August 26, 2025 .. Cell lines and cell culture HEK-293T, MDA-MB-231, MDA-MB-468, BT-549, U2OS, T47D, hTERT-RPE1, HCT116, HCC38 and H1299 cells were obtained from the American Type Culture Collection (ATCC), while Huh7 cells were sourced from the Japanese Collection of Research Bioresources (JCRB).



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    Image Search Results


    FFAR4 activator TUG891 reduces cell growth. ( A - B ) CCK-8 assays showing the effects of increasing concentrations of TUG891 (0–80 µM) on the proliferation of HCT116 and HT29 cells after 48 h. ( C ) Flow cytometric analysis of cell cycle distribution in HCT116 (upper panel) and HT29 (lower panel) cells following treatment with increasing concentrations of TUG891. ( D ) Western blot analysis of Cyclin D1 expression in CRC cells treated with 40 µM TUG891 in HCT116 (upper panel) and HT29 (lower panel) cells. ( E ) Flow cytometric analysis of apoptosis in CRC cells following TUG891 treatment, as assessed by Annexin V/PI staining. ( F ) Validation of FFAR4 knockdown efficiency at both the mRNA and protein levels. ( G ) CCK-8 assays assessing cell proliferation in control, FFAR4 knockdown, TUG891-treated, and combined FFAR4 knockdown plus TUG891 groups. ( H ) Cell cycle analysis showing the effects of FFAR4 knockdown on TUG891-induced G0/G1 phase arrest. Data are presented as the mean ± standard deviation (SD). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons tests. Exact P values are shown for significant differences. ns, not significant ( P ≥ 0.05)

    Journal: Journal of Translational Medicine

    Article Title: FFAR4 negatively regulates colorectal cancer growth via blocking oxidative phosphorylation

    doi: 10.1186/s12967-026-07942-4

    Figure Lengend Snippet: FFAR4 activator TUG891 reduces cell growth. ( A - B ) CCK-8 assays showing the effects of increasing concentrations of TUG891 (0–80 µM) on the proliferation of HCT116 and HT29 cells after 48 h. ( C ) Flow cytometric analysis of cell cycle distribution in HCT116 (upper panel) and HT29 (lower panel) cells following treatment with increasing concentrations of TUG891. ( D ) Western blot analysis of Cyclin D1 expression in CRC cells treated with 40 µM TUG891 in HCT116 (upper panel) and HT29 (lower panel) cells. ( E ) Flow cytometric analysis of apoptosis in CRC cells following TUG891 treatment, as assessed by Annexin V/PI staining. ( F ) Validation of FFAR4 knockdown efficiency at both the mRNA and protein levels. ( G ) CCK-8 assays assessing cell proliferation in control, FFAR4 knockdown, TUG891-treated, and combined FFAR4 knockdown plus TUG891 groups. ( H ) Cell cycle analysis showing the effects of FFAR4 knockdown on TUG891-induced G0/G1 phase arrest. Data are presented as the mean ± standard deviation (SD). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons tests. Exact P values are shown for significant differences. ns, not significant ( P ≥ 0.05)

    Article Snippet: Human colorectal cancer cell lines HCT116 (CCL-247EMT; ATCC) and HT29 (HTB-38; ATCC) were obtained from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China.

    Techniques: CCK-8 Assay, Western Blot, Expressing, Staining, Biomarker Discovery, Knockdown, Control, Cell Cycle Assay, Standard Deviation

    FFAR4 activation promotes glycolysis and is associated with inhibition of oxidative phosphorylation. ( A ) Medium color shift observed in HCT116 cultures treated with increasing concentrations of TUG891. ( B ) TUG891-induced modulation of lactate secretion in HCT116 medium at 40 µM concentration. ( C ) Color shift in HT29 culture medium following treatment with increasing TUG891 concentrations. ( D ) Modulation of medium lactate content in HT29 cells following treatment with 40 µM TUG891. ( E ) Evaluation of TUG891-mediated antitumor effects in HCT116 (upper) and HT29 (lower) cells under pH-stabilized conditions achieved through NaHCO₃ addition or regular medium renewal. ( F ) Lactate metabolic pathway. ( G ) Effects of glucose supplementation on TUG891’s antitumor activity in HCT116 (left) and HT29 (right) cells. ( H ) Effect of 40 µM TUG891 on extracellular acidification rate (ECAR) in HCT116 (left) and HT29 (right) cells. ( I ) Impact of 40 µM TUG891 on oxygen consumption rate (OCR) in HCT116 (left) and HT29 (right) cells. Data are presented as mean ± standard deviation (SD). Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparisons tests. ns, not significant ( P ≥ 0.05)

    Journal: Journal of Translational Medicine

    Article Title: FFAR4 negatively regulates colorectal cancer growth via blocking oxidative phosphorylation

    doi: 10.1186/s12967-026-07942-4

    Figure Lengend Snippet: FFAR4 activation promotes glycolysis and is associated with inhibition of oxidative phosphorylation. ( A ) Medium color shift observed in HCT116 cultures treated with increasing concentrations of TUG891. ( B ) TUG891-induced modulation of lactate secretion in HCT116 medium at 40 µM concentration. ( C ) Color shift in HT29 culture medium following treatment with increasing TUG891 concentrations. ( D ) Modulation of medium lactate content in HT29 cells following treatment with 40 µM TUG891. ( E ) Evaluation of TUG891-mediated antitumor effects in HCT116 (upper) and HT29 (lower) cells under pH-stabilized conditions achieved through NaHCO₃ addition or regular medium renewal. ( F ) Lactate metabolic pathway. ( G ) Effects of glucose supplementation on TUG891’s antitumor activity in HCT116 (left) and HT29 (right) cells. ( H ) Effect of 40 µM TUG891 on extracellular acidification rate (ECAR) in HCT116 (left) and HT29 (right) cells. ( I ) Impact of 40 µM TUG891 on oxygen consumption rate (OCR) in HCT116 (left) and HT29 (right) cells. Data are presented as mean ± standard deviation (SD). Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparisons tests. ns, not significant ( P ≥ 0.05)

    Article Snippet: Human colorectal cancer cell lines HCT116 (CCL-247EMT; ATCC) and HT29 (HTB-38; ATCC) were obtained from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China.

    Techniques: Activation Assay, Inhibition, Phospho-proteomics, Concentration Assay, Activity Assay, Standard Deviation

    TUG891 reduces the NAD⁺/NADH ratio and disrupts mitochondrial redox homeostasis. ( A - B ) Principal-component analysis (PCA) of gene expression data, showing the clustering of gene profiles for control and FFAR4 activation groups in HCT116 cells ( A ) and HT29 cells ( B ). ( C ) Changes in metabolite levels with and without TUG891 treatment in HCT116 cells (upper panel) and HT29 cells (lower panel). Log2(FC) indicates log2-transformed fold change of the TUG891-treated group relative to the control group. ( D - E ) Over-Representation Analysis identified significantly enriched KEGG pathways based on different metabolites in HCT116 cells ( D ) and HT29 cells ( E ). ( F ) Western blot analysis of malate–aspartate shuttle–related enzymes (GOT1, GOT2, MDH1, and MDH2) and OXPHOS proteins in control and TUG891-treated cells. ( G - H ) Measurement of NAD + levels and the NAD + /NADH ratio in HCT116 cells comparing vehicle and FFAR4 activator groups. ( I - J ) Measurement of NAD + levels and the NAD + /NADH ratio in HT29 cells comparing vehicle and FFAR4 activator groups. ( K , L ) Measurement of the cellular ATP/ADP ratio in HCT116 ( K ) and HT29 ( L ) cells comparing vehicle and FFAR4 activator–treated groups. The data were expressed as the mean ± standard deviation (SD)

    Journal: Journal of Translational Medicine

    Article Title: FFAR4 negatively regulates colorectal cancer growth via blocking oxidative phosphorylation

    doi: 10.1186/s12967-026-07942-4

    Figure Lengend Snippet: TUG891 reduces the NAD⁺/NADH ratio and disrupts mitochondrial redox homeostasis. ( A - B ) Principal-component analysis (PCA) of gene expression data, showing the clustering of gene profiles for control and FFAR4 activation groups in HCT116 cells ( A ) and HT29 cells ( B ). ( C ) Changes in metabolite levels with and without TUG891 treatment in HCT116 cells (upper panel) and HT29 cells (lower panel). Log2(FC) indicates log2-transformed fold change of the TUG891-treated group relative to the control group. ( D - E ) Over-Representation Analysis identified significantly enriched KEGG pathways based on different metabolites in HCT116 cells ( D ) and HT29 cells ( E ). ( F ) Western blot analysis of malate–aspartate shuttle–related enzymes (GOT1, GOT2, MDH1, and MDH2) and OXPHOS proteins in control and TUG891-treated cells. ( G - H ) Measurement of NAD + levels and the NAD + /NADH ratio in HCT116 cells comparing vehicle and FFAR4 activator groups. ( I - J ) Measurement of NAD + levels and the NAD + /NADH ratio in HT29 cells comparing vehicle and FFAR4 activator groups. ( K , L ) Measurement of the cellular ATP/ADP ratio in HCT116 ( K ) and HT29 ( L ) cells comparing vehicle and FFAR4 activator–treated groups. The data were expressed as the mean ± standard deviation (SD)

    Article Snippet: Human colorectal cancer cell lines HCT116 (CCL-247EMT; ATCC) and HT29 (HTB-38; ATCC) were obtained from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China.

    Techniques: Gene Expression, Control, Activation Assay, Transformation Assay, Western Blot, Standard Deviation

    a, Schematic of 2D tumor spheroid arrays embedded with M 3 sensors via sacrificial micromolding. b, The sensor integration into the spheroid array was detected by correlated CFM and MR imaging. (bottom; green, alginate). Scale bar, 500 µm. c , Quantitative stress mapping over the 7-day spheroid growth using the M 3 sensor. Three cell lines (HCT116, A549, and MDA-MB-231) ( n = 10, 13, and 11, respectively) were used for comparision. d - g, Stress development during fibrosis of MRC5 spheroids. A schematic ( d ), CFM, and MR images of fibrotic spheroids with or without fibrosis inhibitor, nintedanib ( e ). Scale bar, 100 μm. Normalized T 2 * signals ( f ) and stress ( g ) of the fibrotic spheroids with or without nintedanib treatment ( n = 11 for (-) and n = 8 for (+)). h - k, Stress dynamics under epithelial-mesenchymal transition (EMT) of tumor spheroids. HCT116-vimentin-RFP reporter cells were used to detect EMT. A schematic ( h ), CFM, and MR images of HCT116 spheroids with or without EMT-inducer, 5-azacytidine ( i ). Scale bar, 100 μm. Normalized T 2 * signals ( j ) and stress ( k ) of HCT116 spheroids u with or without 5-azacytidine treatment ( n = 9 for (-) and n = 12 for (+)). Data are presented as mean ± s.d. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test for ( c ), and by two-tailed unpaired Student’s t-test for ( g ) and ( k ). ****P < 0.0001; **P < 0.01; *P < 0.05.

    Journal: bioRxiv

    Article Title: MechanoMR microparticle (M 3 ) sensors reveal dynamic stress loading as a driver of epithelial-mesenchymal transition

    doi: 10.64898/2025.12.10.693598

    Figure Lengend Snippet: a, Schematic of 2D tumor spheroid arrays embedded with M 3 sensors via sacrificial micromolding. b, The sensor integration into the spheroid array was detected by correlated CFM and MR imaging. (bottom; green, alginate). Scale bar, 500 µm. c , Quantitative stress mapping over the 7-day spheroid growth using the M 3 sensor. Three cell lines (HCT116, A549, and MDA-MB-231) ( n = 10, 13, and 11, respectively) were used for comparision. d - g, Stress development during fibrosis of MRC5 spheroids. A schematic ( d ), CFM, and MR images of fibrotic spheroids with or without fibrosis inhibitor, nintedanib ( e ). Scale bar, 100 μm. Normalized T 2 * signals ( f ) and stress ( g ) of the fibrotic spheroids with or without nintedanib treatment ( n = 11 for (-) and n = 8 for (+)). h - k, Stress dynamics under epithelial-mesenchymal transition (EMT) of tumor spheroids. HCT116-vimentin-RFP reporter cells were used to detect EMT. A schematic ( h ), CFM, and MR images of HCT116 spheroids with or without EMT-inducer, 5-azacytidine ( i ). Scale bar, 100 μm. Normalized T 2 * signals ( j ) and stress ( k ) of HCT116 spheroids u with or without 5-azacytidine treatment ( n = 9 for (-) and n = 12 for (+)). Data are presented as mean ± s.d. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test for ( c ), and by two-tailed unpaired Student’s t-test for ( g ) and ( k ). ****P < 0.0001; **P < 0.01; *P < 0.05.

    Article Snippet: HCT116 vimRFP (CCL-247EMT, ATCC) cells were used to establish tumors for in vivo experiments.

    Techniques: Imaging, Two Tailed Test

    a, Schematic of 2D tumor spheroid arrays embedded with M 3 sensors via sacrificial micromolding. b, The sensor integration into the spheroid array was detected by correlated CFM and MR imaging. (bottom; green, alginate). Scale bar, 500 µm. c , Quantitative stress mapping over the 7-day spheroid growth using the M 3 sensor. Three cell lines (HCT116, A549, and MDA-MB-231) ( n = 10, 13, and 11, respectively) were used for comparision. d - g, Stress development during fibrosis of MRC5 spheroids. A schematic ( d ), CFM, and MR images of fibrotic spheroids with or without fibrosis inhibitor, nintedanib ( e ). Scale bar, 100 μm. Normalized T 2 * signals ( f ) and stress ( g ) of the fibrotic spheroids with or without nintedanib treatment ( n = 11 for (-) and n = 8 for (+)). h - k, Stress dynamics under epithelial-mesenchymal transition (EMT) of tumor spheroids. HCT116-vimentin-RFP reporter cells were used to detect EMT. A schematic ( h ), CFM, and MR images of HCT116 spheroids with or without EMT-inducer, 5-azacytidine ( i ). Scale bar, 100 μm. Normalized T 2 * signals ( j ) and stress ( k ) of HCT116 spheroids u with or without 5-azacytidine treatment ( n = 9 for (-) and n = 12 for (+)). Data are presented as mean ± s.d. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test for ( c ), and by two-tailed unpaired Student’s t-test for ( g ) and ( k ). ****P < 0.0001; **P < 0.01; *P < 0.05.

    Journal: bioRxiv

    Article Title: MechanoMR microparticle (M 3 ) sensors reveal dynamic stress loading as a driver of epithelial-mesenchymal transition

    doi: 10.64898/2025.12.10.693598

    Figure Lengend Snippet: a, Schematic of 2D tumor spheroid arrays embedded with M 3 sensors via sacrificial micromolding. b, The sensor integration into the spheroid array was detected by correlated CFM and MR imaging. (bottom; green, alginate). Scale bar, 500 µm. c , Quantitative stress mapping over the 7-day spheroid growth using the M 3 sensor. Three cell lines (HCT116, A549, and MDA-MB-231) ( n = 10, 13, and 11, respectively) were used for comparision. d - g, Stress development during fibrosis of MRC5 spheroids. A schematic ( d ), CFM, and MR images of fibrotic spheroids with or without fibrosis inhibitor, nintedanib ( e ). Scale bar, 100 μm. Normalized T 2 * signals ( f ) and stress ( g ) of the fibrotic spheroids with or without nintedanib treatment ( n = 11 for (-) and n = 8 for (+)). h - k, Stress dynamics under epithelial-mesenchymal transition (EMT) of tumor spheroids. HCT116-vimentin-RFP reporter cells were used to detect EMT. A schematic ( h ), CFM, and MR images of HCT116 spheroids with or without EMT-inducer, 5-azacytidine ( i ). Scale bar, 100 μm. Normalized T 2 * signals ( j ) and stress ( k ) of HCT116 spheroids u with or without 5-azacytidine treatment ( n = 9 for (-) and n = 12 for (+)). Data are presented as mean ± s.d. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test for ( c ), and by two-tailed unpaired Student’s t-test for ( g ) and ( k ). ****P < 0.0001; **P < 0.01; *P < 0.05.

    Article Snippet: HCT116 vimRFP cell line was purchased from ATCC (CCL-247EMT) and grown in McCoy’s medium (Gibco).

    Techniques: Imaging, Two Tailed Test