Journal: iScience

Article Title: PAR2-β-arrestin 2-ERK axis mediates Malassezia globosa -induced IL-17 response by disrupting ZO-1 in keratinocytes

doi: 10.1016/j.isci.2026.115646

Figure Lengend Snippet: Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by CCK8 assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .

Article Snippet: Cell viability was determined with the Cell Counting Kit-8 (CCK8, Beyotime, Shanghai, China), following the manufacturer’s protocol.

Techniques: Infection, Activation Assay, CCK-8 Assay, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay

Journal: JBMR Plus

Article Title: Establishment and characterization of a human pre-osteocyte cell line: hOsteo4-E9

doi: 10.1093/jbmrpl/ziaf163

Figure Lengend Snippet: Characterization of human-derived hOsteo4 subclones. (A) Phase contrast images of 4 hOsteo4 subclones, that is, C7, D8, E9, and C10; (B) cell viability test by CCK8 assay for 4 hOsteo4 subclones and MLO-Y4 cells; N = 3 for each group; (C) immunofluorescence staining of DMP1, FGF23, and sclerostin of 4 hOsteo4 subclones, MLO-Y4 and MC3T3 cells; IgG group were MC3T3 cells and served as a negative background control; (D) western-blot results for Dmp1, Sclerostin, E11, integrin β1, integrin β3, ALP, and Col-I protein expressions from hOsteo4 subclones, MLO-Y4 cells and MC-3 T3 cells; tubulin served as a loading control.

Article Snippet: Cell proliferation and cytotoxicity were evaluated using a Cell Counting Kit-8 (CCK8) assay (Cat# 40203ES76, Yeasen, Shanghai, China), which quantifies cellular metabolic activity by measuring cellular dehydrogenase activity in viable cells.

Techniques: Derivative Assay, CCK-8 Assay, Immunofluorescence, Staining, Control, Western Blot