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cck8  (Beyotime)


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    Beyotime cck8
    Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by <t>CCK8</t> assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .
    Cck8, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 17182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cck8/product/Beyotime
    Average 99 stars, based on 17182 article reviews
    cck8 - by Bioz Stars, 2026-06
    99/100 stars

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    1) Product Images from "PAR2-β-arrestin 2-ERK axis mediates Malassezia globosa -induced IL-17 response by disrupting ZO-1 in keratinocytes"

    Article Title: PAR2-β-arrestin 2-ERK axis mediates Malassezia globosa -induced IL-17 response by disrupting ZO-1 in keratinocytes

    Journal: iScience

    doi: 10.1016/j.isci.2026.115646

    Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by CCK8 assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .
    Figure Legend Snippet: Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by CCK8 assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .

    Techniques Used: Infection, Activation Assay, CCK-8 Assay, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay



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    Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by <t>CCK8</t> assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .
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    Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by <t>CCK8</t> assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .
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    Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by CCK8 assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .

    Journal: iScience

    Article Title: PAR2-β-arrestin 2-ERK axis mediates Malassezia globosa -induced IL-17 response by disrupting ZO-1 in keratinocytes

    doi: 10.1016/j.isci.2026.115646

    Figure Lengend Snippet: Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by CCK8 assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .

    Article Snippet: Cell viability was determined with the Cell Counting Kit-8 (CCK8, Beyotime, Shanghai, China), following the manufacturer’s protocol.

    Techniques: Infection, Activation Assay, CCK-8 Assay, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay