Journal: bioRxiv

Article Title: Side-by-side evaluation of two mouse models for Crimean-Congo Hemorrhagic Fever Virus infection

doi: 10.1101/2025.08.20.671421

Figure Lengend Snippet: IFNAR -/- mice were infected with either 100 TCID 50 CCHFV Afg09 (purple, n = 8) or Hoti (green, n = 6). The clinical score (A) , summarizing appearance, behaviour, body weight (B) , and body temperature (C) , was monitored daily. After reaching the clinical endpoint (score of ≥10 or ≥6 on two consecutive days), the mice were sacrificed or found dead (grey). Dotted lines mark the clinical endpoints.

Article Snippet: Microtiter plates were coated with CCHFV glycoprotein (Gc) protein (The Native Antigen Company, REC31696) diluted to 0.5 μg/ml in phosphate buffered saline (PBS) and incubated for 20 hours at 4°C.

Techniques: Infection

Journal: bioRxiv

Article Title: Side-by-side evaluation of two mouse models for Crimean-Congo Hemorrhagic Fever Virus infection

doi: 10.1101/2025.08.20.671421

Figure Lengend Snippet: IFNAR -/- mice were infected with either 100 TCID 50 CCHFV Afg09 (purple, n = 8) or Hoti (green, n = 6). A: CCHFV genome copies were measured by RT-qPCR in organs and serum samples (day 3 and final) of individual mice. B: Infectious CCHFV were determined by TCID 50 assay in organs and final serum samples of individual mice. Each data point represents a sample from an individual animal, data are shown as the means ± SD. Datasets were analyzed using the Šídák’s multiple comparisons test (A) or Tukey’s multiple comparisons test (B). Asterisks indicate statistical significance as detailed between CCHFV Afg09 and Kosovo Hoti group: ∗p ≤ 0.05; ∗∗∗∗p < 0.0001.

Article Snippet: Microtiter plates were coated with CCHFV glycoprotein (Gc) protein (The Native Antigen Company, REC31696) diluted to 0.5 μg/ml in phosphate buffered saline (PBS) and incubated for 20 hours at 4°C.

Techniques: Infection, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Side-by-side evaluation of two mouse models for Crimean-Congo Hemorrhagic Fever Virus infection

doi: 10.1101/2025.08.20.671421

Figure Lengend Snippet: IFNAR -/- mice were infected with either 100 TCID 50 CCHFV Afg09 (purple) or Hoti (green). A: Final sera (Afg09 n = 6, Hoti n = 6) were analyzed with two virus-specific ELISAs using either inactivated CCHFV, measuring NP-specific antibodies or the major glycoprotein Gc for coating. Monoclonal antibodies detecting Gc or N protein were used as controls (not shown). Dotted lines indicate the respective lower limit of detection. B: Liver enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed in final serum samples (Afg09 n = 5, Hoti n = 3). Dotted lines indicate the physiological range of healthy C57BL/6 mice.

Article Snippet: Microtiter plates were coated with CCHFV glycoprotein (Gc) protein (The Native Antigen Company, REC31696) diluted to 0.5 μg/ml in phosphate buffered saline (PBS) and incubated for 20 hours at 4°C.

Techniques: Infection, Virus, Bioprocessing

Journal: bioRxiv

Article Title: Side-by-side evaluation of two mouse models for Crimean-Congo Hemorrhagic Fever Virus infection

doi: 10.1101/2025.08.20.671421

Figure Lengend Snippet: Liver, spleen and CNS of CCHFV Afg09 (purple) or Hoti-infected (green) IFNAR -/- mice were pathologically examined post-mortem. A: Shown are exemplary images of organ histopathology, H&E: hematoxylin and eosin staining. ISH: In situ Hybridization. Arrows indicate multifocal liver necrosis with loss of tissue architecture and lymphohistiocytic infiltrates. Arrowheads indicate apoptosis in germinal follicle centers. Scale bars: 100 µm. B: Quantification of ISH indicating percentage area of CCHFV-positive organ sections. Each data point represents a sample from an individual animal, horizonal lines represent the means ± SD. Datasets were analyzed using the Šídák’s multiple comparisons test. Asterisks indicate statistical significance as detailed between CCHFV Afg09 and Kosovo Hoti group: ∗∗∗∗p < 0.0001; ns = not significant.

Article Snippet: Microtiter plates were coated with CCHFV glycoprotein (Gc) protein (The Native Antigen Company, REC31696) diluted to 0.5 μg/ml in phosphate buffered saline (PBS) and incubated for 20 hours at 4°C.

Techniques: Infection, Histopathology, Staining, In Situ Hybridization