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anti cb2r  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti cb2r
    Anti Cb2r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cb2/CB2+Antibody/pm42830193-148-19-32
    Average 93 stars, based on 135 article reviews
    anti cb2r - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Restorative Effect of Microalgae Nannochloropsis oceanica Lipid Extract on Phospholipid Metabolism in Keratinocytes Exposed to UVB Radiation.
    Article Snippet: Determination of Expression of Membrane Receptors Protein expression membrane receptors were measured using an enzyme-linked immunosorbent assay (ELISA) (Nunc Immuno MaxiSorp, Thermo Fisher Scientific, Waltham, MA, USA) [92]. .. Plates with attached protein cell lysates were incubated with a blocking solution (5% fat-free dry milk in a carbonate binding buffer) at 4 ◦C for 3 h. Next, the samples were washed with PBS with 0.1% Tween 20, and incubated overnight with an appropriate primary antibody against TRPV1 (host: mouse) (Sigma-Aldrich, St. Louis, MO, USA); CB1 and CB2 (host: mouse) (Santa Cruz Biotechnology, Dallas, TX, USA); and PPARγ (host: rabbit) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Phytocannabinoids influence phospholipid metabolism of melanoma cells: Modulation of in vitro effects of the UVA irradiation.
    Article Snippet: Cell lysates were added to ELISA plate wells (Nunc Immuno Maxi Sorp, Thermo Scientific, Waltham, MA, USA) and incubated at 4 ◦C for 3 h with a blocking solution containing 5 % fat-free dry milk in carbonate-binding buffer. .. After washing with PBS containing 0.1 % Tween 20, the samples were incubated overnight at 4 ◦C with primary antibodies specific to CB1 and CB2 (host: mouse) (Santa Cruz Biotechnology, Dallas, TX, USA), TRPV1 (host: mouse) (Sigma-Aldrich, St. Louis, MO, USA), and PPARγ (host: rabbit) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Effects of ∆-9 tetrahydrocannabinol on the small intestine altered by high fructose diet: A Histopathological study.
    Article Snippet: .. After washing, the sections were kept in the blocking solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min. Then, the sections were incubated overnight at + 4 °C with the following primary antibodies: IL-6 (sc-1265-R, Santa Cruz Biotechnology, Germany, 1/50 dilution), JNK (sc-7345, Santa Cruz Biotechnology, Germany, 1/20 dilution), CB2 (sc-25494, Santa Cruz Biotechnology, Germany, 1/75 dilution), and PCNA (MS-106-P, Thermo Scientific, USA, 1/50 dilution). .. Afterward, the sections were washed with phosphatebuffered saline (PBS), kept in the biotinylated secondary antibody solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min, and washed again.

    Article Title: Tick-borne encephalitis virus transmitted singly and in duo with Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum bacteria by ticks as pathogens modifying lipid metabolism in human blood.
    Article Snippet: .. After washing with PBS supplemented with 0.1% Tween 20, samples were incubated at 4 °C overnight with appropriate primary antibodies against TRPV1 (host: mouse) (Sigma-Aldrich, St. Louis, MO, USA); CB1, CB2, (host: mouse) (Santa Cruz Biotechnology, CA, USA); PPARγ (host: rabbit) (Invitrogen, Waltham, MA, USA). ..

    Article Title: 3-O-Ethyl Ascorbic Acid and Cannabigerol in Modulating the Phospholipid Metabolism of Keratinocytes
    Article Snippet: .. In order to prepare the samples for the analysis purposes, the keratinocyte lysates were coated onto ELISA plates (Nunc Immuno Maxi Sorp, Thermo Scientific, Waltham, MA, USA) with the appropriate primary antibodies (all diluted 1:1000): TRPV1 (Sigma-Aldrich, St. Louis, MO, USA); CB1 and CB2 (Santa Cruz Biotechnology, Dallas, TX, USA) and PPARγ (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and then incubated at 4 °C overnight. ..

    Article Title: Tick-borne encephalitis virus transmitted singly and in duo with Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum bacteria by ticks as pathogens modifying lipid metabolism in human blood
    Article Snippet: .. After washing with PBS supplemented with 0.1% Tween 20, samples were incubated at 4 °C overnight with appropriate primary antibodies against TRPV1 (host: mouse) (Sigma-Aldrich, St. Louis, MO, USA); CB1, CB2, (host: mouse) (Santa Cruz Biotechnology, CA, USA); PPARγ (host: rabbit) (Invitrogen, Waltham, MA, USA). ..

    Article Title: Effects of ∆-9 tetrahydrocannabinol on the small intestine altered by high fructose diet: A Histopathological study
    Article Snippet: .. After washing, the sections were kept in the blocking solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min. Then, the sections were incubated overnight at + 4 °C with the following primary antibodies: IL-6 (sc-1265-R, Santa Cruz Biotechnology, Germany, 1/50 dilution), JNK (sc-7345, Santa Cruz Biotechnology, Germany, 1/20 dilution), CB2 (sc-25494, Santa Cruz Biotechnology, Germany, 1/75 dilution), and PCNA (MS-106-P, Thermo Scientific, USA, 1/50 dilution). .. Afterward, the sections were washed with phosphate-buffered saline (PBS), kept in the biotinylated secondary antibody solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min, and washed again.

    Blocking Assay:

    Article Title: Restorative Effect of Microalgae Nannochloropsis oceanica Lipid Extract on Phospholipid Metabolism in Keratinocytes Exposed to UVB Radiation.
    Article Snippet: Determination of Expression of Membrane Receptors Protein expression membrane receptors were measured using an enzyme-linked immunosorbent assay (ELISA) (Nunc Immuno MaxiSorp, Thermo Fisher Scientific, Waltham, MA, USA) [92]. .. Plates with attached protein cell lysates were incubated with a blocking solution (5% fat-free dry milk in a carbonate binding buffer) at 4 ◦C for 3 h. Next, the samples were washed with PBS with 0.1% Tween 20, and incubated overnight with an appropriate primary antibody against TRPV1 (host: mouse) (Sigma-Aldrich, St. Louis, MO, USA); CB1 and CB2 (host: mouse) (Santa Cruz Biotechnology, Dallas, TX, USA); and PPARγ (host: rabbit) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Effects of ∆-9 tetrahydrocannabinol on the small intestine altered by high fructose diet: A Histopathological study.
    Article Snippet: .. After washing, the sections were kept in the blocking solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min. Then, the sections were incubated overnight at + 4 °C with the following primary antibodies: IL-6 (sc-1265-R, Santa Cruz Biotechnology, Germany, 1/50 dilution), JNK (sc-7345, Santa Cruz Biotechnology, Germany, 1/20 dilution), CB2 (sc-25494, Santa Cruz Biotechnology, Germany, 1/75 dilution), and PCNA (MS-106-P, Thermo Scientific, USA, 1/50 dilution). .. Afterward, the sections were washed with phosphatebuffered saline (PBS), kept in the biotinylated secondary antibody solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min, and washed again.

    Article Title: Effects of ∆-9 tetrahydrocannabinol on the small intestine altered by high fructose diet: A Histopathological study
    Article Snippet: .. After washing, the sections were kept in the blocking solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min. Then, the sections were incubated overnight at + 4 °C with the following primary antibodies: IL-6 (sc-1265-R, Santa Cruz Biotechnology, Germany, 1/50 dilution), JNK (sc-7345, Santa Cruz Biotechnology, Germany, 1/20 dilution), CB2 (sc-25494, Santa Cruz Biotechnology, Germany, 1/75 dilution), and PCNA (MS-106-P, Thermo Scientific, USA, 1/50 dilution). .. Afterward, the sections were washed with phosphate-buffered saline (PBS), kept in the biotinylated secondary antibody solution (Histostain Plus Broad Spectrum Kit, Invitrogen, 859,043, California, USA) for 10 min, and washed again.

    Binding Assay:

    Article Title: Restorative Effect of Microalgae Nannochloropsis oceanica Lipid Extract on Phospholipid Metabolism in Keratinocytes Exposed to UVB Radiation.
    Article Snippet: Determination of Expression of Membrane Receptors Protein expression membrane receptors were measured using an enzyme-linked immunosorbent assay (ELISA) (Nunc Immuno MaxiSorp, Thermo Fisher Scientific, Waltham, MA, USA) [92]. .. Plates with attached protein cell lysates were incubated with a blocking solution (5% fat-free dry milk in a carbonate binding buffer) at 4 ◦C for 3 h. Next, the samples were washed with PBS with 0.1% Tween 20, and incubated overnight with an appropriate primary antibody against TRPV1 (host: mouse) (Sigma-Aldrich, St. Louis, MO, USA); CB1 and CB2 (host: mouse) (Santa Cruz Biotechnology, Dallas, TX, USA); and PPARγ (host: rabbit) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). ..

    other:

    Article Title: Repeated cannabidiol treatment affects neuroplasticity and endocannabinoid signaling in the prefrontal cortex of the Flinders Sensitive Line (FSL) rat model of depression.
    Article Snippet: Delayed therapeutic responses and limited efficacy are the main challenges of existing antidepressant drugs, thereby incentivizing the search for new potential treatments.. Cannabidiol (CBD), non-psychotomimetic component of cannabis, has shown promising antidepressant effects in different rodent models, but its mechanism of action remains unclear.. Herein, we investigated the antidepressant-like effects of repeated CBD treatment on behavior, neuroplasticity markers and lipidomic profile in the prefrontal cortex (PFC) of Flinders Sensitive Line (FSL), a genetic animal model of depression, and their control counterparts Flinders Resistant Line (FRL) rats.

    Enzyme-linked Immunosorbent Assay:

    Article Title: 3-O-Ethyl Ascorbic Acid and Cannabigerol in Modulating the Phospholipid Metabolism of Keratinocytes
    Article Snippet: .. In order to prepare the samples for the analysis purposes, the keratinocyte lysates were coated onto ELISA plates (Nunc Immuno Maxi Sorp, Thermo Scientific, Waltham, MA, USA) with the appropriate primary antibodies (all diluted 1:1000): TRPV1 (Sigma-Aldrich, St. Louis, MO, USA); CB1 and CB2 (Santa Cruz Biotechnology, Dallas, TX, USA) and PPARγ (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and then incubated at 4 °C overnight. ..



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    Image Search Results


    Schematic overview of the study design combining computational and behavioral approaches. Cardamonin, a chalcone derivative, was evaluated for its interaction with cannabinoid receptors CB1 and CB2 using molecular dynamics simulations and MM/PBSA analyses. Parallel in vivo behavioral assaysVon Frey and Hargreaves testswere conducted in mice to assess mechanical and thermal antinociception, respectively.

    Journal: ACS Omega

    Article Title: Receptor-Selective Modulation of Cannabinoid Signaling by Cardamonin: Integrating Molecular Dynamics, Free Energy Calculations, and Behavioral Validation

    doi: 10.1021/acsomega.5c10026

    Figure Lengend Snippet: Schematic overview of the study design combining computational and behavioral approaches. Cardamonin, a chalcone derivative, was evaluated for its interaction with cannabinoid receptors CB1 and CB2 using molecular dynamics simulations and MM/PBSA analyses. Parallel in vivo behavioral assaysVon Frey and Hargreaves testswere conducted in mice to assess mechanical and thermal antinociception, respectively.

    Article Snippet: Cardamonin (MedChemExpress LLC, USA) and selective cannabinoid receptor 1 (CB1) antagonists SR141716 (MedChemExpress LLC, USA) and selective cannabinoid receptor 2 (CB2) antagonist SR144528 (MedChemExpress LLC, USA) were dissolved in dimethyl sulfoxide [DMSO], Tween 20 and diluted with saline at a ratio of 5:5:90 for intraperitoneal (i.p.) administration.

    Techniques: In Vivo

    Docking poses of cardamonin with CB1 (A) and CB2 (B) receptors. (A) Cardamonin binds to CB1 with −8.7 kcal/mol, forming hydrogen bonds (SER 505 , ILE 267 ), hydrophobic contacts (PHE 170 , VAL 196 , LEU 193 , PHE 200 ), and a π-cation interaction (HIS 178 ). (B) In CB2 (−8.0 kcal/mol), cardamonin engages in hydrophobic interactions and a π-stacking (PHE117). Interaction types: hydrogen bonds (blue), hydrophobic (gray dashed), π-cation (orange dashed), π-stacking (green dashed).

    Journal: ACS Omega

    Article Title: Receptor-Selective Modulation of Cannabinoid Signaling by Cardamonin: Integrating Molecular Dynamics, Free Energy Calculations, and Behavioral Validation

    doi: 10.1021/acsomega.5c10026

    Figure Lengend Snippet: Docking poses of cardamonin with CB1 (A) and CB2 (B) receptors. (A) Cardamonin binds to CB1 with −8.7 kcal/mol, forming hydrogen bonds (SER 505 , ILE 267 ), hydrophobic contacts (PHE 170 , VAL 196 , LEU 193 , PHE 200 ), and a π-cation interaction (HIS 178 ). (B) In CB2 (−8.0 kcal/mol), cardamonin engages in hydrophobic interactions and a π-stacking (PHE117). Interaction types: hydrogen bonds (blue), hydrophobic (gray dashed), π-cation (orange dashed), π-stacking (green dashed).

    Article Snippet: Cardamonin (MedChemExpress LLC, USA) and selective cannabinoid receptor 1 (CB1) antagonists SR141716 (MedChemExpress LLC, USA) and selective cannabinoid receptor 2 (CB2) antagonist SR144528 (MedChemExpress LLC, USA) were dissolved in dimethyl sulfoxide [DMSO], Tween 20 and diluted with saline at a ratio of 5:5:90 for intraperitoneal (i.p.) administration.

    Techniques:

    Hydrogen bond interaction snapshots between cardamonin and cannabinoid receptors CB1 (A) and CB2 (B) at selected time points from molecular dynamics simulations. Each frame corresponds to a representative structure at 100, 250, 500, and 750 ns. Hydrogen bonds are depicted as red dashed lines with annotated distances (Å). In CB1, dynamic repositioning of the ligand allows alternating interactions with residues such as HSD 178 , ILE 267 , PHE 189 , ASP 184 , SER 173 , and LEU 193 . In contrast, the CB2 complex demonstrates a more consistent hydrogen bonding profile, particularly with SER 285 and GLY 284 . These interactions reflect the temporal stability and flexibility of ligand–receptor engagement during the simulation trajectory.

    Journal: ACS Omega

    Article Title: Receptor-Selective Modulation of Cannabinoid Signaling by Cardamonin: Integrating Molecular Dynamics, Free Energy Calculations, and Behavioral Validation

    doi: 10.1021/acsomega.5c10026

    Figure Lengend Snippet: Hydrogen bond interaction snapshots between cardamonin and cannabinoid receptors CB1 (A) and CB2 (B) at selected time points from molecular dynamics simulations. Each frame corresponds to a representative structure at 100, 250, 500, and 750 ns. Hydrogen bonds are depicted as red dashed lines with annotated distances (Å). In CB1, dynamic repositioning of the ligand allows alternating interactions with residues such as HSD 178 , ILE 267 , PHE 189 , ASP 184 , SER 173 , and LEU 193 . In contrast, the CB2 complex demonstrates a more consistent hydrogen bonding profile, particularly with SER 285 and GLY 284 . These interactions reflect the temporal stability and flexibility of ligand–receptor engagement during the simulation trajectory.

    Article Snippet: Cardamonin (MedChemExpress LLC, USA) and selective cannabinoid receptor 1 (CB1) antagonists SR141716 (MedChemExpress LLC, USA) and selective cannabinoid receptor 2 (CB2) antagonist SR144528 (MedChemExpress LLC, USA) were dissolved in dimethyl sulfoxide [DMSO], Tween 20 and diluted with saline at a ratio of 5:5:90 for intraperitoneal (i.p.) administration.

    Techniques:

    Principal Component Analysis (PCA) of CB1 and CB2 receptor complexes. (A) PCA projection of CB1 control (black) and cardamonin-bound complex (red) onto the first two principal components. Cardamonin binding induces broader conformational sampling. (B) PCA projection of CB2 control (black) and cardamonin-bound complex (red), showing overlapping conformational space and reduced ligand impact.

    Journal: ACS Omega

    Article Title: Receptor-Selective Modulation of Cannabinoid Signaling by Cardamonin: Integrating Molecular Dynamics, Free Energy Calculations, and Behavioral Validation

    doi: 10.1021/acsomega.5c10026

    Figure Lengend Snippet: Principal Component Analysis (PCA) of CB1 and CB2 receptor complexes. (A) PCA projection of CB1 control (black) and cardamonin-bound complex (red) onto the first two principal components. Cardamonin binding induces broader conformational sampling. (B) PCA projection of CB2 control (black) and cardamonin-bound complex (red), showing overlapping conformational space and reduced ligand impact.

    Article Snippet: Cardamonin (MedChemExpress LLC, USA) and selective cannabinoid receptor 1 (CB1) antagonists SR141716 (MedChemExpress LLC, USA) and selective cannabinoid receptor 2 (CB2) antagonist SR144528 (MedChemExpress LLC, USA) were dissolved in dimethyl sulfoxide [DMSO], Tween 20 and diluted with saline at a ratio of 5:5:90 for intraperitoneal (i.p.) administration.

    Techniques: Control, Binding Assay, Sampling

    (A) Mechanical paw withdrawal threshold (mean ± SEM) in the Von Frey test. SHM and CDM groups displayed high thresholds (normal sensitivity), while VHC, CB1 – , and CB2 – groups exhibited mechanical allodynia. Co-treatment with cardamonin (CDM+CB1 – and CDM+CB2 – ) partially restored pain thresholds. (B) Thermal paw withdrawal latency (mean ± SEM) in the Hargreaves test. SHM group showed normal latency. CB1 – , CB2 – , and VHC groups exhibited thermal hyperalgesia. Cardamonin (CDM) increased latency significantly, and cotreatment with CB1 – or CB2 – partially restored thermal sensitivity.

    Journal: ACS Omega

    Article Title: Receptor-Selective Modulation of Cannabinoid Signaling by Cardamonin: Integrating Molecular Dynamics, Free Energy Calculations, and Behavioral Validation

    doi: 10.1021/acsomega.5c10026

    Figure Lengend Snippet: (A) Mechanical paw withdrawal threshold (mean ± SEM) in the Von Frey test. SHM and CDM groups displayed high thresholds (normal sensitivity), while VHC, CB1 – , and CB2 – groups exhibited mechanical allodynia. Co-treatment with cardamonin (CDM+CB1 – and CDM+CB2 – ) partially restored pain thresholds. (B) Thermal paw withdrawal latency (mean ± SEM) in the Hargreaves test. SHM group showed normal latency. CB1 – , CB2 – , and VHC groups exhibited thermal hyperalgesia. Cardamonin (CDM) increased latency significantly, and cotreatment with CB1 – or CB2 – partially restored thermal sensitivity.

    Article Snippet: Cardamonin (MedChemExpress LLC, USA) and selective cannabinoid receptor 1 (CB1) antagonists SR141716 (MedChemExpress LLC, USA) and selective cannabinoid receptor 2 (CB2) antagonist SR144528 (MedChemExpress LLC, USA) were dissolved in dimethyl sulfoxide [DMSO], Tween 20 and diluted with saline at a ratio of 5:5:90 for intraperitoneal (i.p.) administration.

    Techniques: