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pro caspase3 antibody  (Boster Bio)


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    Structured Review

    Boster Bio pro caspase3 antibody
    Pro Caspase3 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase3/Anti-Caspase+3+Rabbit+Monoclonal+Antibody/pmc13085978-28-0-3
    Average 90 stars, based on 51 article reviews
    pro caspase3 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Dietary Supplementation with Selenium Yeast Mitigates Diquat-Induced Oxidative Damage in Oviductal Magnum of Hens
    Article Snippet: Proteins were resolved on SDS-PAGE (12% separating, 5% stacking gel) and transferred to PVDF membranes (0.45 μm) (Millipore Sigma, Bedford, MA, USA). .. After blocking, membranes were incubated overnight at 4°C with primary antibodies (Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), ZP2 (PB0958, Boster, Wuhan, China), Wnt4 (A00879, Boster, Wuhan, China)) and β-actin (BM0627, Boster, Wuhan, China) as a control. ..

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters.
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4◦C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Article Title: Selenium yeast alleviates diquat-induced oxidative stress and testicular damage in roosters.
    Article Snippet: Diquat (DQ) is a pro-oxidant that generates free radicals in cells through redox reactions, leading to the induction of oxidative stress.. During the processes of growth and reproduction, poultry are particularly vulnerable to oxidative stress.. Selenium yeast (SeY) serves as an organic selenium source characterized by high activity and low toxicity, imparting antioxidant effects.

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Incubation:

    Article Title: Dietary Supplementation with Selenium Yeast Mitigates Diquat-Induced Oxidative Damage in Oviductal Magnum of Hens
    Article Snippet: Proteins were resolved on SDS-PAGE (12% separating, 5% stacking gel) and transferred to PVDF membranes (0.45 μm) (Millipore Sigma, Bedford, MA, USA). .. After blocking, membranes were incubated overnight at 4°C with primary antibodies (Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), ZP2 (PB0958, Boster, Wuhan, China), Wnt4 (A00879, Boster, Wuhan, China)) and β-actin (BM0627, Boster, Wuhan, China) as a control. ..

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters.
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4◦C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Article Title: Liqi Huatan Huoxue formula alleviates coronary microvascular dysfunction via NRG-1/PI3K/AKT pathway
    Article Snippet: Background: Coronary microvascular dysfunction (CMD) is a common cardiac disease that impairs patients’ life quality substantially.. Traditional Chinese medicine (TCM) demonstrates distinctive therapeutic potential in the management of CMD.. Purpose: This study aimed to analyze the chemical profile and blood-absorved constituents of Liqi Huatan Huoxue (LQHTHX) formula, and to evaluate its therapeutic effects on CMD through both in vivo and in vitro experiments.

    Article Title: Tetrachlorantraniliprole induces neurodevelopmental toxicity through oxidative stress-mediated apoptosis and dysregulation of Wnt signaling pathway.
    Article Snippet: Tetrachlorantraniliprole (TCTP) is a novel bisamide insecticide and widely used to protect against lepidopteran insect species.. However, the application of TCTP in rice fields often leads to water pollution, posing threats to aquatic organisms and potentially to human health.. Few studies have assessed the toxic effects of TCTP on aquatic animals.

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Control:

    Article Title: Dietary Supplementation with Selenium Yeast Mitigates Diquat-Induced Oxidative Damage in Oviductal Magnum of Hens
    Article Snippet: Proteins were resolved on SDS-PAGE (12% separating, 5% stacking gel) and transferred to PVDF membranes (0.45 μm) (Millipore Sigma, Bedford, MA, USA). .. After blocking, membranes were incubated overnight at 4°C with primary antibodies (Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), ZP2 (PB0958, Boster, Wuhan, China), Wnt4 (A00879, Boster, Wuhan, China)) and β-actin (BM0627, Boster, Wuhan, China) as a control. ..

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters.
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4◦C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Saline:

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters.
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4◦C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10 % SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Sigma, Bedford, MA, USA). .. After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China). .. Following three washes with TBST, membranes were incubated with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Servicebio, Wuhan, China) for 1 h at room temperature.

    Membrane:

    Article Title: Tetrachlorantraniliprole induces neurodevelopmental toxicity through oxidative stress-mediated apoptosis and dysregulation of Wnt signaling pathway.
    Article Snippet: Tetrachlorantraniliprole (TCTP) is a novel bisamide insecticide and widely used to protect against lepidopteran insect species.. However, the application of TCTP in rice fields often leads to water pollution, posing threats to aquatic organisms and potentially to human health.. Few studies have assessed the toxic effects of TCTP on aquatic animals.



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    Cell Signaling Technology Inc rabbit anti cleaved caspase3 antibody
    Comprehensive functional validation of CTT Platform after cryopreservation and simulated transport. A) Schematic illustration of the experimental workflow. Fresh or cryopreserved PM@NSC (at −80 °C or −196 °C for 3 months) were thawed and subjected to a 4-h simulated transport at 4 °C prior to in vitro analysis or in vivo transplantation for SCI repair. B) Representative confocal microscopy images assessing post-thaw cell cytoskeletal integrity of NSCs loaded onto PM. Phalloidin (green) for F-actin; DAPI (blue) for nucleus. Scale bar: 50 μm. C) Western blot bands of Nestin, Sox2, and Ki67 show no significant differences in protein expression levels among control (fresh), cryopreserved (−80 °C/-196 °C), and cryopreservation (−80 °C/-196 °C)-transport groups. D) Representative confocal microscopy images assessing post-thaw cell viability of NSCs loaded onto PM. Calcein AM (green) for live cells; PI (red) for dead cells. Scale bar: 50 μm. E) Western blot bands of <t>Cleaved-Caspase3,</t> Bcl-2, and Bax protein expression in protein expression levels among control (fresh), cryopreserved (−80 °C/-196 °C), and cryopreservation (−80 °C/-196 °C)-transport groups. F) Quantitative analysis of cell survival rate of NSC in each group (n = 5). G) Quantitative analysis of Nestin/GAPDH, Ki67/GAPDH, and Sox2/β-Actin ratios in each group (n = 3). H) Quantitative analysis of Cleaved-Caspase3/GAPDH, Bcl-2/GAPDH and Bax/GAPDH ratios in each group (n = 3). I) Representative photographs of rat hindlimb motor functions in each group, 8 weeks after SCI. J) MEP results show variations in latency and amplitude in the left hind leg of each group 56 days after SCI K) H&E staining of gastrocnemius muscles indicated variations in muscle fiber morphology among the groups 56 days after SCI. Scale bar: 200 μm L) Sagittal and axial T2-weighted MRI images of rats in each group 56 days after SCI. M) Footprint analysis with print views, footfall patterns, 3D footprints, and 2D footprints revealing differences in gait patterns among separate groups. N) BBB scores demonstrate comparable locomotor functional recovery across all groups, including the Control group and the Cryopreserved-Transport treated group (n = 5). O) Quantitative analysis of gastrocnemius muscle fiber cross-sectional area, indicating similar muscle functional recovery (n = 5). P) Quantitative analysis of MEP latency and amplitude in the left hind leg in each group (n = 5). Q) Quantitative analysis of T2 density in sagittal and coronal planes in spinal cord lesions among groups (n = 5). R) Quantitative footprint analysis on day 56 post-injury included the maximum footprint intensity, footprint positioning, and the regularity index of the left hindlimb (n = 10). All data are presented as the mean ± SEM. Statistical analysis showed no significant differences (n.s.) among the experimental groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    Effects of HAP on cartilage autophagy and apoptosis in KOA model rats. Note: (A,C–F) Expression of LC3 I, LC3 II, Beclin1, <t>C-Caspase3</t> and Bax in cartilage tissue (GAPDH as internal control). (B,G,H) Expression of p62 and Bcl2 in cartilage (GAPDH as internal control). *** p < 0.001, ** p < 0.01, * p < 0.05.
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    Effects of HAP on cartilage autophagy and apoptosis in KOA model rats. Note: (A,C–F) Expression of LC3 I, LC3 II, Beclin1, <t>C-Caspase3</t> and Bax in cartilage tissue (GAPDH as internal control). (B,G,H) Expression of p62 and Bcl2 in cartilage (GAPDH as internal control). *** p < 0.001, ** p < 0.01, * p < 0.05.
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    Image Search Results


    Comprehensive functional validation of CTT Platform after cryopreservation and simulated transport. A) Schematic illustration of the experimental workflow. Fresh or cryopreserved PM@NSC (at −80 °C or −196 °C for 3 months) were thawed and subjected to a 4-h simulated transport at 4 °C prior to in vitro analysis or in vivo transplantation for SCI repair. B) Representative confocal microscopy images assessing post-thaw cell cytoskeletal integrity of NSCs loaded onto PM. Phalloidin (green) for F-actin; DAPI (blue) for nucleus. Scale bar: 50 μm. C) Western blot bands of Nestin, Sox2, and Ki67 show no significant differences in protein expression levels among control (fresh), cryopreserved (−80 °C/-196 °C), and cryopreservation (−80 °C/-196 °C)-transport groups. D) Representative confocal microscopy images assessing post-thaw cell viability of NSCs loaded onto PM. Calcein AM (green) for live cells; PI (red) for dead cells. Scale bar: 50 μm. E) Western blot bands of Cleaved-Caspase3, Bcl-2, and Bax protein expression in protein expression levels among control (fresh), cryopreserved (−80 °C/-196 °C), and cryopreservation (−80 °C/-196 °C)-transport groups. F) Quantitative analysis of cell survival rate of NSC in each group (n = 5). G) Quantitative analysis of Nestin/GAPDH, Ki67/GAPDH, and Sox2/β-Actin ratios in each group (n = 3). H) Quantitative analysis of Cleaved-Caspase3/GAPDH, Bcl-2/GAPDH and Bax/GAPDH ratios in each group (n = 3). I) Representative photographs of rat hindlimb motor functions in each group, 8 weeks after SCI. J) MEP results show variations in latency and amplitude in the left hind leg of each group 56 days after SCI K) H&E staining of gastrocnemius muscles indicated variations in muscle fiber morphology among the groups 56 days after SCI. Scale bar: 200 μm L) Sagittal and axial T2-weighted MRI images of rats in each group 56 days after SCI. M) Footprint analysis with print views, footfall patterns, 3D footprints, and 2D footprints revealing differences in gait patterns among separate groups. N) BBB scores demonstrate comparable locomotor functional recovery across all groups, including the Control group and the Cryopreserved-Transport treated group (n = 5). O) Quantitative analysis of gastrocnemius muscle fiber cross-sectional area, indicating similar muscle functional recovery (n = 5). P) Quantitative analysis of MEP latency and amplitude in the left hind leg in each group (n = 5). Q) Quantitative analysis of T2 density in sagittal and coronal planes in spinal cord lesions among groups (n = 5). R) Quantitative footprint analysis on day 56 post-injury included the maximum footprint intensity, footprint positioning, and the regularity index of the left hindlimb (n = 10). All data are presented as the mean ± SEM. Statistical analysis showed no significant differences (n.s.) among the experimental groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair

    doi: 10.1016/j.bioactmat.2026.01.024

    Figure Lengend Snippet: Comprehensive functional validation of CTT Platform after cryopreservation and simulated transport. A) Schematic illustration of the experimental workflow. Fresh or cryopreserved PM@NSC (at −80 °C or −196 °C for 3 months) were thawed and subjected to a 4-h simulated transport at 4 °C prior to in vitro analysis or in vivo transplantation for SCI repair. B) Representative confocal microscopy images assessing post-thaw cell cytoskeletal integrity of NSCs loaded onto PM. Phalloidin (green) for F-actin; DAPI (blue) for nucleus. Scale bar: 50 μm. C) Western blot bands of Nestin, Sox2, and Ki67 show no significant differences in protein expression levels among control (fresh), cryopreserved (−80 °C/-196 °C), and cryopreservation (−80 °C/-196 °C)-transport groups. D) Representative confocal microscopy images assessing post-thaw cell viability of NSCs loaded onto PM. Calcein AM (green) for live cells; PI (red) for dead cells. Scale bar: 50 μm. E) Western blot bands of Cleaved-Caspase3, Bcl-2, and Bax protein expression in protein expression levels among control (fresh), cryopreserved (−80 °C/-196 °C), and cryopreservation (−80 °C/-196 °C)-transport groups. F) Quantitative analysis of cell survival rate of NSC in each group (n = 5). G) Quantitative analysis of Nestin/GAPDH, Ki67/GAPDH, and Sox2/β-Actin ratios in each group (n = 3). H) Quantitative analysis of Cleaved-Caspase3/GAPDH, Bcl-2/GAPDH and Bax/GAPDH ratios in each group (n = 3). I) Representative photographs of rat hindlimb motor functions in each group, 8 weeks after SCI. J) MEP results show variations in latency and amplitude in the left hind leg of each group 56 days after SCI K) H&E staining of gastrocnemius muscles indicated variations in muscle fiber morphology among the groups 56 days after SCI. Scale bar: 200 μm L) Sagittal and axial T2-weighted MRI images of rats in each group 56 days after SCI. M) Footprint analysis with print views, footfall patterns, 3D footprints, and 2D footprints revealing differences in gait patterns among separate groups. N) BBB scores demonstrate comparable locomotor functional recovery across all groups, including the Control group and the Cryopreserved-Transport treated group (n = 5). O) Quantitative analysis of gastrocnemius muscle fiber cross-sectional area, indicating similar muscle functional recovery (n = 5). P) Quantitative analysis of MEP latency and amplitude in the left hind leg in each group (n = 5). Q) Quantitative analysis of T2 density in sagittal and coronal planes in spinal cord lesions among groups (n = 5). R) Quantitative footprint analysis on day 56 post-injury included the maximum footprint intensity, footprint positioning, and the regularity index of the left hindlimb (n = 10). All data are presented as the mean ± SEM. Statistical analysis showed no significant differences (n.s.) among the experimental groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The primary antibodies used in this research are listed below: CD68 (Abcam, Cambridge, UK), CD206 (Abcam, Cambridge, UK), GFAP (Bioss, Beijing, China), iNOS (Abcam, Cambridge, UK), Tuj-1 (Abcam, Cambridge, UK), NF-200 (Invitrogen, CA, USA), MBP (Abcam, Cambridge, UK), HIF-1α (Abcam, Cambridge, UK), VEGFA (Abcam, Cambridge, UK), P-CaMKII (Abcam, Cambridge, UK), CaMKII (Abcam, Cambridge, UK), P-CREB (Cell Signaling Technology, USA), CREB (Cell Signaling Technology, USA), P-PI3K (Cell Signaling Technology, USA), PI3K (Cell Signaling Technology, USA), P-AKT (Cell Signaling Technology, USA), AKT (Cell Signaling Technology, USA), Cleaved-Caspase3 (Cell Signaling Technology, USA), Bcl-2 (Cell Signaling Technology, USA), Bax (Cell Signaling Technology, USA), GAPDH (Proteintech, IL, USA).

    Techniques: Functional Assay, Biomarker Discovery, In Vitro, In Vivo, Transplantation Assay, Confocal Microscopy, Western Blot, Expressing, Control, Staining, Muscles

    Effects of HAP on cartilage autophagy and apoptosis in KOA model rats. Note: (A,C–F) Expression of LC3 I, LC3 II, Beclin1, C-Caspase3 and Bax in cartilage tissue (GAPDH as internal control). (B,G,H) Expression of p62 and Bcl2 in cartilage (GAPDH as internal control). *** p < 0.001, ** p < 0.01, * p < 0.05.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Uncovering the mechanisms of homologous point acupuncture on knee osteoarthritis through an integrated study of metabolomics and proteomics

    doi: 10.3389/fbioe.2026.1791109

    Figure Lengend Snippet: Effects of HAP on cartilage autophagy and apoptosis in KOA model rats. Note: (A,C–F) Expression of LC3 I, LC3 II, Beclin1, C-Caspase3 and Bax in cartilage tissue (GAPDH as internal control). (B,G,H) Expression of p62 and Bcl2 in cartilage (GAPDH as internal control). *** p < 0.001, ** p < 0.01, * p < 0.05.

    Article Snippet: All the primary antibodies were validated by either the manufacturer or other available publications, listed as follows: HIF1α (Boster, A00013-1), HK2 (Abclonal, A0994), c-caspase3 (Affinity, AF7022), Bax (Wuhan Sanying, 50599-2-Ig), LC3 (CST, 12741T), Beclin1 (Wuhan Sanying, 11306-1-AP), and GAPDH (Wuhan Sanying, 60004-1-Ig) from WUHAN HUAYAN Biotechnology CO., LTD. NLRP3 (Affinity, BF8029), ASC (Affinity, DF6304), caspase1 (Affinity, AF5418), p62 (Wuhan Sanying, 18420-1-AP), Bcl2 (Wuhan Sanying, 60178-1-Ig) from WUHAN Fabre Biotechnology CO., LTD. After washing 5 times for 5 min each with TBST (T-Pro), and a horseradish peroxidase (HRP)-conjugated secondary antibody (Boster, BA1051 and BA1054) was applied for 2 h at room temperature.

    Techniques: Expressing, Control