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rabbit anti caspase 8 cell death  (Proteintech)


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    Structured Review

    Proteintech rabbit anti caspase 8 cell death
    Rabbit Anti Caspase 8 Cell Death, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+8/Caspase+8%2Fp43%2Fp18+Antibody/10__1038_slash_s41419___026___09312___w-367-19-27
    Average 96 stars, based on 401 article reviews
    rabbit anti caspase 8 cell death - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Shear stress-induced Ca 2+ influx triggers endoplasmic reticulum stress and cardiomyocyte apoptosis: implications for mitral regulation
    Article Snippet: After protein separation, the gels were transferred onto Immobilon-E PVDF Membranes (Millipore, IEVH85R) and blocked with F1 1-min Blocking Buffer Plus (Lion Biotechnologies Inc., F1BLOK500P). .. The samples were analyzed using antibodies against Grp78 (BD, 610978), ATF6 (Santa Cruz, sc166659), p-PERK (Cell Signaling, 3179), PERK (Cell Signaling, 3192), p-IRE1α (Abcam, ab48187), IRE1α (Abcam, ab37073), p-eIF2α (Cell Signaling, 9721), eIF2α (Cell Signaling, 9722), ATF4 (Santa Cruz, sc390063), CHOP (Cell Signaling, 2895), Bax (Genetex, GTX109683), Bcl-2 (BD, 610538), Bak (Cell Signaling, 12105), caspase 9 (Santa Cruz, sc56076), caspase 12 (Cell Signaling, 35965), caspase 3 (Abcam, ab32042), caspase 8 (Proteintech, 13423-1-AP), Bid (Novus, 10988-1-AP), and transient receptor potential canonical 1 (TRPC1) (Santa Cruz, sc133076). .. GAPDH (Abcam, ab8245) was used as a loading control.

    Incubation:

    Article Title: Ginsenoside Rg3 synergizes with near-infrared photothermal therapy to suppress prostate cancer progression by inhibiting RAS signaling and enhancing ARL11-mediated macrophage reprogramming.
    Article Snippet: Prostate cancer (PCa) progression and therapeutic resistance are largely driven by aberrant oncogenic signaling and an immunosuppressive microenvironment.. Ginsenoside Rg3, a natural saponin from Panax ginseng, exhibits antitumor and immunomodulatory activity, but its therapeutic efficacy is limited when used as a monotherapy.. Here, we investigated the synergistic potential of combining Rg3 with near-infrared (NIR) exposure in PCa.

    Article Title: Microsatellite instability detection in extracellular vesicles of short-chain fatty acids-treated colorectal cancer cells
    Article Snippet: .. After electrophoresis, proteins were transferred onto PVDF membranes using wet transfer at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in PBS-T for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against CD63 (1:500, Santa Cruz), Alix (1:500, Thermo Fisher Scientific), Calnexin (1:5000, Abcam), Caspase-3 (1:1000, R&D Systems), and Caspase-8 (1:2000, Proteintech). ..

    Article Title: Macrophage‐derived galectin‐3 contributes to pyroptosis, apoptosis and necroptosis through TLR4/MyD88/NF‐κB/NLRP3 during atherosclerosis
    Article Snippet: .. Membranes were then incubated overnight on a shaker with primary mouse or rabbit antibodies against galectin‐3 (60207‐1‐Ig, Proteintech, China), GSDMD (AF4012, Affinity Biosciences, China), NLRP3 (DF7438, Affinity Biosciences, China), caspase‐3 (66470‐2‐lg, Proteintech, China), caspase‐8 (66093‐1‐Ig, Proteintech, China), RIPK3 (A5431, ABclonal, China), MLKL (A26436, ABclonal, China), Phospho‐MLKL (AP0949, ABclonal, China), TLR4 (GB11519, Servicebio, China), MyD88 (GB12269, Servicebio, China), NF‐κB (10745‐1‐AP, Proteintech, China), Phospho‐NF‐κB ( GB113882 , Servicebio, China) and GAPDH (60004‐1‐Ig, Proteintech, China). ..

    CCK-8 Assay:

    Article Title: Combined Treatment with Shuyu Pills and Everolimus Suppresses Triple-Negative Breast Cancer Growth via the PI3K/AKT/mTOR Pathway
    Article Snippet: Reagents The DMEM culture medium was purchased from Gibco (USA); fetal bovine serum (FBS) was obtained from Biological Industries (BI, Israel); penicillin-streptomycin solution was purchased from HyClone (USA). .. The CCK-8 assay kit and Annexin V-FITC/PI apoptosis detection kit were obtained from MeilunBio®(China), Ltd. High-efficiency RIPA lysis buffer, dimethyl sulfoxide (DMSO), and SDS-PAGE gel rapid preparation kit were purchased from Beijing Solarbio Science & Technology Co, Ltd. Primary antibodies including β-actin (81115-1-RR), Bax (50599-2-Ig), Bcl-2 (80,313- 1-RR), Caspase-3 (82,202-1-RR), Caspase-8 (40,930), Cyclin B (55004-1-AP), and CDK1 (19,532-1-AP) were obtained from Proteintech Group, Inc(China), Ltd. Antibodies against PI3K (ab40776), mTOR (ab32028), and AKT (ab38449) were purchased from Abcam (UK), while the phosphorylated forms p-PI3K (341468), p-mTOR (381557), and p-AKT (381555) were provided by Chengdu Zenon Biotechnology Co., Ltd. Matrigel (Cat. No. 356234) was purchased from BD Biosciences (USA). https://doi.org/10.2147/BCTT.S580570 Breast Cancer: Targets and Therapy 2026:18 2 Powered by TCPDF (www.tcpdf.org) Drug Preparation The herbal formulation SYP was used in a modified form based on the original prescription. .. The granules were supplied by Guangdong Yifang Pharmaceutical Co., Ltd., and were prepared according to the equivalent dose conversion from crude herbal slices.

    Lysis:

    Article Title: Combined Treatment with Shuyu Pills and Everolimus Suppresses Triple-Negative Breast Cancer Growth via the PI3K/AKT/mTOR Pathway
    Article Snippet: Reagents The DMEM culture medium was purchased from Gibco (USA); fetal bovine serum (FBS) was obtained from Biological Industries (BI, Israel); penicillin-streptomycin solution was purchased from HyClone (USA). .. The CCK-8 assay kit and Annexin V-FITC/PI apoptosis detection kit were obtained from MeilunBio®(China), Ltd. High-efficiency RIPA lysis buffer, dimethyl sulfoxide (DMSO), and SDS-PAGE gel rapid preparation kit were purchased from Beijing Solarbio Science & Technology Co, Ltd. Primary antibodies including β-actin (81115-1-RR), Bax (50599-2-Ig), Bcl-2 (80,313- 1-RR), Caspase-3 (82,202-1-RR), Caspase-8 (40,930), Cyclin B (55004-1-AP), and CDK1 (19,532-1-AP) were obtained from Proteintech Group, Inc(China), Ltd. Antibodies against PI3K (ab40776), mTOR (ab32028), and AKT (ab38449) were purchased from Abcam (UK), while the phosphorylated forms p-PI3K (341468), p-mTOR (381557), and p-AKT (381555) were provided by Chengdu Zenon Biotechnology Co., Ltd. Matrigel (Cat. No. 356234) was purchased from BD Biosciences (USA). https://doi.org/10.2147/BCTT.S580570 Breast Cancer: Targets and Therapy 2026:18 2 Powered by TCPDF (www.tcpdf.org) Drug Preparation The herbal formulation SYP was used in a modified form based on the original prescription. .. The granules were supplied by Guangdong Yifang Pharmaceutical Co., Ltd., and were prepared according to the equivalent dose conversion from crude herbal slices.

    SDS Page:

    Article Title: Combined Treatment with Shuyu Pills and Everolimus Suppresses Triple-Negative Breast Cancer Growth via the PI3K/AKT/mTOR Pathway
    Article Snippet: Reagents The DMEM culture medium was purchased from Gibco (USA); fetal bovine serum (FBS) was obtained from Biological Industries (BI, Israel); penicillin-streptomycin solution was purchased from HyClone (USA). .. The CCK-8 assay kit and Annexin V-FITC/PI apoptosis detection kit were obtained from MeilunBio®(China), Ltd. High-efficiency RIPA lysis buffer, dimethyl sulfoxide (DMSO), and SDS-PAGE gel rapid preparation kit were purchased from Beijing Solarbio Science & Technology Co, Ltd. Primary antibodies including β-actin (81115-1-RR), Bax (50599-2-Ig), Bcl-2 (80,313- 1-RR), Caspase-3 (82,202-1-RR), Caspase-8 (40,930), Cyclin B (55004-1-AP), and CDK1 (19,532-1-AP) were obtained from Proteintech Group, Inc(China), Ltd. Antibodies against PI3K (ab40776), mTOR (ab32028), and AKT (ab38449) were purchased from Abcam (UK), while the phosphorylated forms p-PI3K (341468), p-mTOR (381557), and p-AKT (381555) were provided by Chengdu Zenon Biotechnology Co., Ltd. Matrigel (Cat. No. 356234) was purchased from BD Biosciences (USA). https://doi.org/10.2147/BCTT.S580570 Breast Cancer: Targets and Therapy 2026:18 2 Powered by TCPDF (www.tcpdf.org) Drug Preparation The herbal formulation SYP was used in a modified form based on the original prescription. .. The granules were supplied by Guangdong Yifang Pharmaceutical Co., Ltd., and were prepared according to the equivalent dose conversion from crude herbal slices.

    Formulation:

    Article Title: Combined Treatment with Shuyu Pills and Everolimus Suppresses Triple-Negative Breast Cancer Growth via the PI3K/AKT/mTOR Pathway
    Article Snippet: Reagents The DMEM culture medium was purchased from Gibco (USA); fetal bovine serum (FBS) was obtained from Biological Industries (BI, Israel); penicillin-streptomycin solution was purchased from HyClone (USA). .. The CCK-8 assay kit and Annexin V-FITC/PI apoptosis detection kit were obtained from MeilunBio®(China), Ltd. High-efficiency RIPA lysis buffer, dimethyl sulfoxide (DMSO), and SDS-PAGE gel rapid preparation kit were purchased from Beijing Solarbio Science & Technology Co, Ltd. Primary antibodies including β-actin (81115-1-RR), Bax (50599-2-Ig), Bcl-2 (80,313- 1-RR), Caspase-3 (82,202-1-RR), Caspase-8 (40,930), Cyclin B (55004-1-AP), and CDK1 (19,532-1-AP) were obtained from Proteintech Group, Inc(China), Ltd. Antibodies against PI3K (ab40776), mTOR (ab32028), and AKT (ab38449) were purchased from Abcam (UK), while the phosphorylated forms p-PI3K (341468), p-mTOR (381557), and p-AKT (381555) were provided by Chengdu Zenon Biotechnology Co., Ltd. Matrigel (Cat. No. 356234) was purchased from BD Biosciences (USA). https://doi.org/10.2147/BCTT.S580570 Breast Cancer: Targets and Therapy 2026:18 2 Powered by TCPDF (www.tcpdf.org) Drug Preparation The herbal formulation SYP was used in a modified form based on the original prescription. .. The granules were supplied by Guangdong Yifang Pharmaceutical Co., Ltd., and were prepared according to the equivalent dose conversion from crude herbal slices.

    Modification:

    Article Title: Combined Treatment with Shuyu Pills and Everolimus Suppresses Triple-Negative Breast Cancer Growth via the PI3K/AKT/mTOR Pathway
    Article Snippet: Reagents The DMEM culture medium was purchased from Gibco (USA); fetal bovine serum (FBS) was obtained from Biological Industries (BI, Israel); penicillin-streptomycin solution was purchased from HyClone (USA). .. The CCK-8 assay kit and Annexin V-FITC/PI apoptosis detection kit were obtained from MeilunBio®(China), Ltd. High-efficiency RIPA lysis buffer, dimethyl sulfoxide (DMSO), and SDS-PAGE gel rapid preparation kit were purchased from Beijing Solarbio Science & Technology Co, Ltd. Primary antibodies including β-actin (81115-1-RR), Bax (50599-2-Ig), Bcl-2 (80,313- 1-RR), Caspase-3 (82,202-1-RR), Caspase-8 (40,930), Cyclin B (55004-1-AP), and CDK1 (19,532-1-AP) were obtained from Proteintech Group, Inc(China), Ltd. Antibodies against PI3K (ab40776), mTOR (ab32028), and AKT (ab38449) were purchased from Abcam (UK), while the phosphorylated forms p-PI3K (341468), p-mTOR (381557), and p-AKT (381555) were provided by Chengdu Zenon Biotechnology Co., Ltd. Matrigel (Cat. No. 356234) was purchased from BD Biosciences (USA). https://doi.org/10.2147/BCTT.S580570 Breast Cancer: Targets and Therapy 2026:18 2 Powered by TCPDF (www.tcpdf.org) Drug Preparation The herbal formulation SYP was used in a modified form based on the original prescription. .. The granules were supplied by Guangdong Yifang Pharmaceutical Co., Ltd., and were prepared according to the equivalent dose conversion from crude herbal slices.

    other:

    Article Title: Shear stress-induced Ca 2+ influx triggers endoplasmic reticulum stress and cardiomyocyte apoptosis: implications for mitral regulation.
    Article Snippet: We quantified the protein content of the samples and then separated the proteins based on molecular weight via sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) on 7.5, 12.5, and 15% gels using an electrophoresis tank (Bio-Rad).

    Electrophoresis:

    Article Title: Microsatellite instability detection in extracellular vesicles of short-chain fatty acids-treated colorectal cancer cells
    Article Snippet: .. After electrophoresis, proteins were transferred onto PVDF membranes using wet transfer at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in PBS-T for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against CD63 (1:500, Santa Cruz), Alix (1:500, Thermo Fisher Scientific), Calnexin (1:5000, Abcam), Caspase-3 (1:1000, R&D Systems), and Caspase-8 (1:2000, Proteintech). ..

    Western Blot:

    Article Title: Riboflavin photochemical therapy triggered PANoptosis by inducing mitochondrial damage in circulating colorectal cancer cells.
    Article Snippet: The antibodies used for flow cytometry were: FITC-CD3 (#344804, 1:200 dilution), FITC-IFN-γ (#502506, 1:100 dilution), PE-CD107a (#328608, 1:100 dilution), PerCP-CD3 (#300428, 1:200 dilution), PECy7-granzyme B (#372214, 1:100 dilution), PE-Cy7-CD45 (#304016, 1:200 dilution), APC-CD19 (#302212, 1:200 dilution), APC-CD4 (#300537, 1:200 dilution), APC-CD8 (#344722, 1:200 dilution), APCCD56 (#362504, 1:200 dilution), APC IgG2b (#402205, 1:200 dilution), APC-CD326 (#324208, 1:200 dilution), APCCy7-CD56 (#318332, 1:200 dilution) from Biolegend; LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit from Thermo Fisher Scientific (#65-0865-14, 1:1000 dilution); PE-Annexin V (#559763, 1:100 dilution), APC-Annexin V (#550474, 1:100 dilution) from BD Biosciences. .. The antibodies used for western blotting were: Vinculin (#26520-1-AP, 1:5000 dilution), Caspase-3 (#19677-1-AP, 1:1000 dilution), Caspase-1 (22915-1-AP, 1:1000 dilution), Caspase-7 (#27155-1-AP, 1:1000 dilution), Caspase-8 (#13423-1- AP, 1:1000 dilution), Caspase-9 (#10380-1-AP, 1:1000 dilution), GSDME (#13075-1-AP, 1:1000 dilution) from Proteintech; GSDMD (#GB115429, 1:1000 dilution) from Servicebio; MLKL (#ab184718, 1:1000 dilution), Phospho-MLKL (#ab196436, 1:1000 dilution), TFRC (#ab214039, 1:1000 dilution) from Abcam; RIP1 (#3493, 1:1000 dilution), Phospho-RIP1 (#65746, 1:1000 dilution) from Cell Signaling Technology. .. Antibodies for DNA (#NB110-89473, 1:200 dilution) was purchased from Novus.



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