Cell Culture:Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli
Article Snippet: .. HTB-44 kidney epithelial cells were cultured as previously described ( 36 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant genotype/phenotype Reference Strains CFT073 Parent strain (ATCC 700928) ATCC 700928 CFT073ΔompX ompX mutant of CFT073 This work CFT073ΔfliC fliC mutant of CFT073 11 CFT073ΔfliCΔompX fliC and ompX double mutant of CFT073 This work Plasmids pKO3 Temperature-sensitive vector for gene targeting, sacB , Cm r 37 pTrc99K Vector for IPTG-inducible expression; Km r 38 pTrc99KompX ompX expression plasmid; Km r This work pTrc99A Vector for IPTG-inducible expression; Ap r 38 pTrc99AompX ompX expression plasmid; Ap r This work pTrc99AfliC fliC expression plasmid; Ap r This work pTH18kr Low-copy-no. plasmid; Km r 39 pTH18krfliC-VSVG C-terminally VSVG-tagged FliC expression plasmid; Km r This work pTH18krompX ompX expression plasmid; Km r This work pNN387 Single-copy plasmid with promoterless lacZ ; Cm r 40 pNNflhD-P flhD promoter reporter; Cm r This work pNNfliA-P fliA promoter reporter; Cm r This work pNNfliC-P fliC promoter reporter; Cm r This work pTurboGFP-B GFP expression plasmid; Ap r Evrogen Open in a separate window a Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Ap r , ampicillin resistance. ..
Plasmid Preparation:Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli
Article Snippet: .. HTB-44 kidney epithelial cells were cultured as previously described ( 36 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant genotype/phenotype Reference Strains CFT073 Parent strain (ATCC 700928) ATCC 700928 CFT073ΔompX ompX mutant of CFT073 This work CFT073ΔfliC fliC mutant of CFT073 11 CFT073ΔfliCΔompX fliC and ompX double mutant of CFT073 This work Plasmids pKO3 Temperature-sensitive vector for gene targeting, sacB , Cm r 37 pTrc99K Vector for IPTG-inducible expression; Km r 38 pTrc99KompX ompX expression plasmid; Km r This work pTrc99A Vector for IPTG-inducible expression; Ap r 38 pTrc99AompX ompX expression plasmid; Ap r This work pTrc99AfliC fliC expression plasmid; Ap r This work pTH18kr Low-copy-no. plasmid; Km r 39 pTH18krfliC-VSVG C-terminally VSVG-tagged FliC expression plasmid; Km r This work pTH18krompX ompX expression plasmid; Km r This work pNN387 Single-copy plasmid with promoterless lacZ ; Cm r 40 pNNflhD-P flhD promoter reporter; Cm r This work pNNfliA-P fliA promoter reporter; Cm r This work pNNfliC-P fliC promoter reporter; Cm r This work pTurboGFP-B GFP expression plasmid; Ap r Evrogen Open in a separate window a Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Ap r , ampicillin resistance. ..
Article Title: A Riboflavin Transporter in Bdellovibrio exovorous JSS
Article Snippet: .. Bacterial strains, plasmids, media and culture conditions Bacterial strains and plasmids used in this study are listed in . table ft1 table-wrap mode="anchored" t5 Table 1- caption a7 Strain or Plasmid Relevant characteristics Source Bdellovibrio exovorous JSS Wild type ATCC BAA-2330 Caulobacter crescentus CB15N Wild type SDSU, Dr. Shapiro Escherichia coli BW25113 Wild type In house E. coli BSV13 Δ ribC CGSC Plasmid PKD13 Amp + Kan + Temp - In house Plasmid PCP20 Amp + Kan + Temp - In house Open in a separate window Strains and plasmids used in this study. .. Initial studies on the growth of B. exovorous strain JSS (ATCC BAA-2330) were conducted in co-cultures in 1/10-strength yeast extract-peptone medium supplemented with calcium [ Gordon et al., 1993 ].
Article Title: A Riboflavin Transporter in Bdellovibrio exovorous JSS
Article Snippet: .. Bacterial strains and plasmids used in this study are listed in . table ft1 table-wrap mode="anchored" t5 Table 1- caption a7 Strain or Plasmid Relevant characteristics Source Bdellovibrio exovorous JSS Wild type ATCC BAA-2330 Caulobacter crescentus CB15N Wild type SDSU, Dr. Shapiro Escherichia coli BW25113 Wild type In house E. coli BSV13 Δ ribC CGSC Plasmid PKD13 Amp + Kan + Temp - In house Plasmid PCP20 Amp + Kan + Temp - In house Open in a separate window Strains and plasmids used in this study. .. Initial studies on the growth of B. exovorous strain JSS (ATCC BAA-2330) were conducted in co-cultures in 1/10-strength yeast extract-peptone medium supplemented with calcium [ Gordon et al., 1993 ].
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study Screen for spontaneous desiccation-sensitive mutants and genomic analysis. ..
Mutagenesis:Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli
Article Snippet: .. HTB-44 kidney epithelial cells were cultured as previously described ( 36 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant genotype/phenotype Reference Strains CFT073 Parent strain (ATCC 700928) ATCC 700928 CFT073ΔompX ompX mutant of CFT073 This work CFT073ΔfliC fliC mutant of CFT073 11 CFT073ΔfliCΔompX fliC and ompX double mutant of CFT073 This work Plasmids pKO3 Temperature-sensitive vector for gene targeting, sacB , Cm r 37 pTrc99K Vector for IPTG-inducible expression; Km r 38 pTrc99KompX ompX expression plasmid; Km r This work pTrc99A Vector for IPTG-inducible expression; Ap r 38 pTrc99AompX ompX expression plasmid; Ap r This work pTrc99AfliC fliC expression plasmid; Ap r This work pTH18kr Low-copy-no. plasmid; Km r 39 pTH18krfliC-VSVG C-terminally VSVG-tagged FliC expression plasmid; Km r This work pTH18krompX ompX expression plasmid; Km r This work pNN387 Single-copy plasmid with promoterless lacZ ; Cm r 40 pNNflhD-P flhD promoter reporter; Cm r This work pNNfliA-P fliA promoter reporter; Cm r This work pNNfliC-P fliC promoter reporter; Cm r This work pTurboGFP-B GFP expression plasmid; Ap r Evrogen Open in a separate window a Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Ap r , ampicillin resistance. ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study Screen for spontaneous desiccation-sensitive mutants and genomic analysis. ..
Expressing:Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli
Article Snippet: .. HTB-44 kidney epithelial cells were cultured as previously described ( 36 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant genotype/phenotype Reference Strains CFT073 Parent strain (ATCC 700928) ATCC 700928 CFT073ΔompX ompX mutant of CFT073 This work CFT073ΔfliC fliC mutant of CFT073 11 CFT073ΔfliCΔompX fliC and ompX double mutant of CFT073 This work Plasmids pKO3 Temperature-sensitive vector for gene targeting, sacB , Cm r 37 pTrc99K Vector for IPTG-inducible expression; Km r 38 pTrc99KompX ompX expression plasmid; Km r This work pTrc99A Vector for IPTG-inducible expression; Ap r 38 pTrc99AompX ompX expression plasmid; Ap r This work pTrc99AfliC fliC expression plasmid; Ap r This work pTH18kr Low-copy-no. plasmid; Km r 39 pTH18krfliC-VSVG C-terminally VSVG-tagged FliC expression plasmid; Km r This work pTH18krompX ompX expression plasmid; Km r This work pNN387 Single-copy plasmid with promoterless lacZ ; Cm r 40 pNNflhD-P flhD promoter reporter; Cm r This work pNNfliA-P fliA promoter reporter; Cm r This work pNNfliC-P fliC promoter reporter; Cm r This work pTurboGFP-B GFP expression plasmid; Ap r Evrogen Open in a separate window a Cm r , chloramphenicol resistance; Km r , kanamycin resistance; Ap r , ampicillin resistance. ..
other:Article Title: Analyzing Possible Native Functions of the Quinolone Resistance Gene qnr in Vibrio vulnificus
Article Snippet: MICs for all the tested agents were the same at 37°C and 15°C except for bile salts, which were lower at 15°C ( ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or genotype MIC (μg/ml) Bile salts (%) at 37°C/15°C H 2 O 2 (mM) Ciprofloxacin Levofloxacin Novobiocin Coumermycin A1 Mitomycin C 4-Nitroquinoline- N -oxide 5-Azacytidine V. vulnificus ATCC 27562 0.031 0.063 1 0.5 0.25 2 >512 0.63/0.25 1 Δ qnrVv 0.004 0.016 1 0.5 0.063 2 >512 0.63/0.13 1 Δ cspA 0.031 0.063 2 1 0.25 2 >512 0.63/0.25 1 Δ qnrVv Δ cspA 0.004 0.016 2 1 0.031 2 >512 0.63/0.13 1 V. vulnificus ATCC 27562/pQE60 0.063 0.063 1 1 0.125 2 >512 0.63/0.25 1 V. vulnificus ATCC 27562/pET-Duet-1 0.063 0.063 0.5 1 0.125 2 >512 0.63/0.25 1 Δ qnrVv /pQE60- qnrVv 0.063 0.063 0.5 0.5 0.125 2 >512 1.25/0.13 1 Δ cspA /pQE60- cspA 0.063 0.063 1 1 0.125 2 >512 1.25/0.25 1 Δ qnrVv Δ cspA /pET-Duet-1- qnrVv - cspA 0.063 0.063 1 1 0.125 2 >512 1.25/0.13 1 Δ qnrVv /pQE60 0.008 0.016 1 1 0.063 2 >512 0.63/0.25 1 Δ cspA /pQE60 0.063 0.063 1 2 0.25 2 >512 0.63/0.25 1 Δ qnrVv Δ cspA /pET-Duet-1 0.008 0.016 1 2 0.063 2 >512 0.63/0.25 1 Open in a separate window MIC values for the different Vibrio vulnificus ATCC 27562 strains
Selection:Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study Screen for spontaneous desiccation-sensitive mutants and genomic analysis. ..
Derivative Assay:Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study Screen for spontaneous desiccation-sensitive mutants and genomic analysis. ..
Sequencing:Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study Screen for spontaneous desiccation-sensitive mutants and genomic analysis. ..
Clone Assay:Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study ..
Article Title: CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii
Article Snippet: .. Antibiotics used for selection were purchased from Research Products International and were used to supplement media at the concentrations indicated in the text. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Description Reference or source Strains ATCC 17961 Clinical isolate from blood ATCC AB09-003 Military isolate from cerebrospinal fluid 11 ATCC 17978 Clinical isolate from spinal meningitis ATCC AB5075 Military isolate from osteomyelitis 58 AB003-04D4 Laboratory-adapted isolate derived from AB09-003 This study AB003-14F2 Laboratory-adapted isolate derived from AB09-003 This study AB003-01C11 Laboratory-adapted isolate derived from AB09-003 This study AB003-02F8 Laboratory-adapted isolate derived from AB09-003 This study AB003-02H10 Laboratory-adapted isolate derived from AB09-003 This study 17961-Δ14.5kb Mutant derived from strain ATCC 17961 with approx 14.5 kb of sequence downstream from csrA deleted This study 17961-Δ csrA csrA deletion mutant derived from strain ATCC 17961 This study 17961-Δ csrA comp 17961-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study AB09-003-Δ csrA csrA deletion mutant derived from strain AB09-003 This study AB09-003-Δ csrA comp AB09-003-Δ csrA with an intact copy of csrA integrated at the att Tn7 site This study Plasmids pEX18Ap Suicide vector 80 pEX18Gm Suicide vector This study pΔ14.5-dsr961 Suicide plasmid carrying approx 14.5-kb deletion This study pEX18Ap-csrA-FL pEX18Ap carrying full-length csrA This study pΔcsrA-suc Suicide plasmid carrying csrA deletion This study pΔcsrA-sucGm Suicide plasmid carrying csrA deletion This study pWH1266 Escherichia coli - A. baumannii shuttle plasmid 45 pJF-csrA csrA cloned into pWH1266 This study pUC18T-mini-Tn7T-Gm Vector for integration of DNA fragments at chromosomal att Tn7 site 44 pJF-Tn7T-csrA csrA cloned into pUC18T-mini-Tn7T-Gm This study pTNS2 Helper plasmid 44 pFLP2A Plasmid encoding Flp recombinase 78 Open in a separate window A. baumannii strains and plasmids used in this study Screen for spontaneous desiccation-sensitive mutants and genomic analysis. ..
|
ATCC is a verified supplier 
ATCC manufactures this product 
