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rep cap genes paav2 9n  (Addgene inc)


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    Structured Review

    Addgene inc rep cap genes paav2 9n
    Rep Cap Genes Paav2 9n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cap+genes/pAAV2%2F9n+(Plasmid+%23112865)/bio_rxiv__2025__10__10__681747-284-21-27
    Average 96 stars, based on 201 article reviews
    rep cap genes paav2 9n - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice.
    Article Snippet: Our recent work on the prion protein and Na+, K+-ATPases (NKAs) led us to revisit data from over 50 years ago, which suggested similarities between vacuolation phenotypes in rodents poisoned with cardiac glycosides (CGs) and spongiform degeneration in prion disease.. At that time, this hypothesis was dismissed because the vacuolation observed in prion diseases affects neurons, whereas CG poisoning in rodent brains led to swellings of the endoplasmic reticulum (ER) in astrocytes.. We speculated that this difference might be specific to rodents and document here that the vacuolation shifts to neurons in mice expressing a humanized NKA α1 subunit.

    Article Title: Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice
    Article Snippet: Briefly, HEK293T cells were grown in a HYPERFlask with 1,720 cm2 surface area and 560 mL growth medium (CLS10031-4EA, MilliporeSigma). .. At 70- 80% confluency, the cells were triply transfected with the rAAV transfer plasmid, the rAAV packaging plasmid coding for Rep and Cap genes (catalog number 103005, Addgene), which we modified by SDM to code for the 9P31 capsid, and the helper plasmid coding for adenovirus genes E2A, E4 and VA (catalog number 112867, Addgene). .. Five days later, 3 mL Triton-X 100 (catalog number X100-100ML, MilliporeSigma), 250 μL RNAse A (catalog number EN0531, Thermo Fisher Scientific), 56 μL Pluronic F-68 (catalog number 24040-032, Thermo Fisher Scientific), and 56 μL turbonuclease (catalog number T4330, MilliporeSigma) were added to the cell culture medium in the HYPERFlask.

    Plasmid Preparation:

    Article Title: Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice.
    Article Snippet: Our recent work on the prion protein and Na+, K+-ATPases (NKAs) led us to revisit data from over 50 years ago, which suggested similarities between vacuolation phenotypes in rodents poisoned with cardiac glycosides (CGs) and spongiform degeneration in prion disease.. At that time, this hypothesis was dismissed because the vacuolation observed in prion diseases affects neurons, whereas CG poisoning in rodent brains led to swellings of the endoplasmic reticulum (ER) in astrocytes.. We speculated that this difference might be specific to rodents and document here that the vacuolation shifts to neurons in mice expressing a humanized NKA α1 subunit.

    Article Title: Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice
    Article Snippet: Briefly, HEK293T cells were grown in a HYPERFlask with 1,720 cm2 surface area and 560 mL growth medium (CLS10031-4EA, MilliporeSigma). .. At 70- 80% confluency, the cells were triply transfected with the rAAV transfer plasmid, the rAAV packaging plasmid coding for Rep and Cap genes (catalog number 103005, Addgene), which we modified by SDM to code for the 9P31 capsid, and the helper plasmid coding for adenovirus genes E2A, E4 and VA (catalog number 112867, Addgene). .. Five days later, 3 mL Triton-X 100 (catalog number X100-100ML, MilliporeSigma), 250 μL RNAse A (catalog number EN0531, Thermo Fisher Scientific), 56 μL Pluronic F-68 (catalog number 24040-032, Thermo Fisher Scientific), and 56 μL turbonuclease (catalog number T4330, MilliporeSigma) were added to the cell culture medium in the HYPERFlask.

    Modification:

    Article Title: Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice.
    Article Snippet: Our recent work on the prion protein and Na+, K+-ATPases (NKAs) led us to revisit data from over 50 years ago, which suggested similarities between vacuolation phenotypes in rodents poisoned with cardiac glycosides (CGs) and spongiform degeneration in prion disease.. At that time, this hypothesis was dismissed because the vacuolation observed in prion diseases affects neurons, whereas CG poisoning in rodent brains led to swellings of the endoplasmic reticulum (ER) in astrocytes.. We speculated that this difference might be specific to rodents and document here that the vacuolation shifts to neurons in mice expressing a humanized NKA α1 subunit.

    Article Title: Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice
    Article Snippet: Briefly, HEK293T cells were grown in a HYPERFlask with 1,720 cm2 surface area and 560 mL growth medium (CLS10031-4EA, MilliporeSigma). .. At 70- 80% confluency, the cells were triply transfected with the rAAV transfer plasmid, the rAAV packaging plasmid coding for Rep and Cap genes (catalog number 103005, Addgene), which we modified by SDM to code for the 9P31 capsid, and the helper plasmid coding for adenovirus genes E2A, E4 and VA (catalog number 112867, Addgene). .. Five days later, 3 mL Triton-X 100 (catalog number X100-100ML, MilliporeSigma), 250 μL RNAse A (catalog number EN0531, Thermo Fisher Scientific), 56 μL Pluronic F-68 (catalog number 24040-032, Thermo Fisher Scientific), and 56 μL turbonuclease (catalog number T4330, MilliporeSigma) were added to the cell culture medium in the HYPERFlask.



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    The specificity analysis of △Cap-iELISA against the common pathgens in geese. The reference anti-serum samples included GoCV positive anti-serum (GoCV-positive), GoCV negative anti-serum (GoCV-negative), Newcastle disease virus positive anti-serum (NDV), Goose parvovirus positive anti-serum (GPV), Goose paramyxovirus positive anti-serum (GPMV), Tembusu virus positive anti-serum (TMUV), Avian reovirus positive anti-serum (ARV) and H5 and H7 subtypes of Avian influenza virus positive anti-serum (AIH5, AIH7). **** for p < 0.0001.

    Journal: Frontiers in Veterinary Science

    Article Title: Epidemiological investigation of goose circovirus based on a newly developed indirect ELISA method

    doi: 10.3389/fvets.2025.1521705

    Figure Lengend Snippet: The specificity analysis of △Cap-iELISA against the common pathgens in geese. The reference anti-serum samples included GoCV positive anti-serum (GoCV-positive), GoCV negative anti-serum (GoCV-negative), Newcastle disease virus positive anti-serum (NDV), Goose parvovirus positive anti-serum (GPV), Goose paramyxovirus positive anti-serum (GPMV), Tembusu virus positive anti-serum (TMUV), Avian reovirus positive anti-serum (ARV) and H5 and H7 subtypes of Avian influenza virus positive anti-serum (AIH5, AIH7). **** for p < 0.0001.

    Article Snippet: The △Cap gene (37aa-250aa) of strain GoCV/369/GD/2020 (GenBank Accession No. MT831925, referred to as GoCV/369) with the nuclear localization sequence (NLS) removed was synthesized by Genscript (Nanjing, China) using codon optimization software (GenSmart TM Codon Optimization) (the modified sequence is included in the ).

    Techniques: Virus