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Journal: PLOS One
Article Title: Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model
doi: 10.1371/journal.pone.0346246
Figure Lengend Snippet: Number of ChAT + (A-C), GAD-67 + (D), Calbindin + (E), Parvalbumin + (F), Parvalbumin + −Calbindin + (G), Parvalbumin + −GAD-67 + (H), and ChAT + −GAD-67 + (I) cells per biological replicate. The number of cells analysed is the sum of immunostained positive cells from eight spinal cord cross-sections (two sections per lumbar level L3–L6) per biological replicate. Data are shown as mean ± standard deviation and were analysed by an ordinary one-way ANOVA. N = 3 biological replicates per genotype.
Article Snippet: Following three 10-min PBS washes at 100 rpm, secondary probing was performed for 3 hours in the dark in PBS containing 0.05% Tween-20 and the relevant secondary antibodies for ChAT (1:250 dilution, donkey anti-goat IgG H + L Alexa Fluor ® 405, Abcam, ab175664),
Techniques: Standard Deviation