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hmvec cells  (Cell Applications Inc)


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    Cell Applications Inc hmvec cells
    Hmvec Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cadmec/Human+Dermal+Microvascular+Endothelial+Cells%3A+CADMEC%2FHMVEC/us11701366-233-15-17
    Average 94 stars, based on 38 article reviews
    hmvec cells - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: HWBAO62 polypeptides
    Article Snippet: The Growth Medium is then replaced with 300 mg Cell Applications' Chord Formation Medium containing control buffer or a polypeptide of the invention (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Article Title: Antibodies to HNFIP24 polypeptides
    Article Snippet: The Growth Medium is then replaced with 300 mg Cell Applications' Chord Formation Medium containing control buffer or a polypeptide of the invention (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Article Title: Antibodies to HT5GJ57
    Article Snippet: The Growth Medium is then replaced with 300 mg Cell Applications' Chord Formation Medium containing control buffer or a polypeptide of the invention (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    In Vitro:

    Article Title: Antibodies to HWHGU54 polypeptides
    Article Snippet: CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 ml/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium. .. The Growth Medium is then replaced with 300 mg Cell Applications' Chord Formation Medium containing control buffer or a polypeptide of the invention (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Article Title: Methods of inhibiting proliferation or immunoglobulin production of B cells using human tumor necrosis factor receptor TR-17 polypeptides
    Article Snippet: CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 μl/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium. .. The Growth Medium is then replaced with 300 μg Cell Applications' Chord Formation Medium containing control buffer or TR17 (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Angiogenesis Assay:

    Article Title: Antibodies to HWHGU54 polypeptides
    Article Snippet: CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 ml/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium. .. The Growth Medium is then replaced with 300 mg Cell Applications' Chord Formation Medium containing control buffer or a polypeptide of the invention (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Article Title: Methods of inhibiting proliferation or immunoglobulin production of B cells using human tumor necrosis factor receptor TR-17 polypeptides
    Article Snippet: CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 μl/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium. .. The Growth Medium is then replaced with 300 μg Cell Applications' Chord Formation Medium containing control buffer or TR17 (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Cell Culture:

    Article Title: Antibodies to HWHGU54 polypeptides
    Article Snippet: CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 ml/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium. .. The Growth Medium is then replaced with 300 mg Cell Applications' Chord Formation Medium containing control buffer or a polypeptide of the invention (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.

    Article Title: Death domain containing receptor 4 antibodies and methods
    Article Snippet: .. CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 μl/well) for 30 minutes at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium.

    Article Title: Splice variants of human G-protein coupled receptor HGPRBMY29 (HGPRMBY29SV2)
    Article Snippet: .. CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 ml/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium.

    Article Title: 12 human secreted proteins
    Article Snippet: .. CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 ml/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium.

    Article Title: Methods of inhibiting proliferation or immunoglobulin production of B cells using human tumor necrosis factor receptor TR-17 polypeptides
    Article Snippet: CADMEC (microvascular endothelial cells) are purchased from Cell Applications, Inc. as proliferating (passage 2) cells and are cultured in Cell Applications' CADMEC Growth Medium and used at passage 5. .. For the in vitro angiogenesis assay, the wells of a 48-well cell culture plate are coated with Cell Applications' Attachment Factor Medium (200 μl/well) for 30 min. at 37° C. CADMEC are seeded onto the coated wells at 7,500 cells/well and cultured overnight in Growth Medium. .. The Growth Medium is then replaced with 300 μg Cell Applications' Chord Formation Medium containing control buffer or TR17 (0.1 to 100 ng/ml) and the cells are cultured for an additional 48 hr.



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    Image Search Results


    Port-wine stain (PWS) endothelial cells (ECs) showed dual arterial and venous identities of co-expression of Eph receptor B1 (EphB1) and ephrin B2 (EfnB2). (a, b) PWS ECs were EphB1+ and EfnB2+. (c, d) A normal dermal venule showed expression of EphB1 but not EfnB2. (e) PWS ECs were EphB4−. (f) Relative mRNA levels of EphB1 and EfnB2 in selected normal human dermal microvascular endothelial cell (hDMVEC) subtypes. (g–i) Forced co-expression of EphB1 and EfnB2 in normal hDMVECs showed a significant increase in (g) branch thickness, (h) branch point area and (i) tube circumference of the capillary tubes formed in vitro compared with controls. (j) PWS blood vessel-like phenotypes were observed in EphB1+/EfnB2+ but not in wild-type and EphB1+/EGFP+ control hDMVECs in an in vitro capillary tube formation assay at 12 h after cell plating. Positive stain is diaminobenzidine (DAB; brown). Scale bar = 100 μm. *P < 0·05, ** P < 0·01 vs. control. The arrows indicate blood vessels. (k) Left panel, detection of expression of EphB1, EphB4, β-actin and EfnB2 by Western blot (W.B.) in various hDMVEC subpopulations. hDMVEC, heterogeneous population prior to EfnB2–Fc selection; EphB1− hDMVEC, the remaining hDMVEC subpopulation after EfnB2–Fc selection; enhanced green fluorescent protein (EGFP) or EfnB2–His, overexpression of EGFP or EfnB2 in the EfnB2–Fc selected hDMVEC subpopulation. Right panel, an anti-EphB1 antibody was used to immunoprecipitate EphB1 from cell lysate and EfnB2 was detected from the immunoprecipitated protein complex using an anti-EfnB2 or anti-His antibody.

    Journal: The British journal of dermatology

    Article Title: Coexistence of Eph receptor B 1 and ephrin B 2 in port-wine stain endothelial progenitor cells contributes to clinicopathological vasculature dilatation

    doi: 10.1111/bjd.15716

    Figure Lengend Snippet: Port-wine stain (PWS) endothelial cells (ECs) showed dual arterial and venous identities of co-expression of Eph receptor B1 (EphB1) and ephrin B2 (EfnB2). (a, b) PWS ECs were EphB1+ and EfnB2+. (c, d) A normal dermal venule showed expression of EphB1 but not EfnB2. (e) PWS ECs were EphB4−. (f) Relative mRNA levels of EphB1 and EfnB2 in selected normal human dermal microvascular endothelial cell (hDMVEC) subtypes. (g–i) Forced co-expression of EphB1 and EfnB2 in normal hDMVECs showed a significant increase in (g) branch thickness, (h) branch point area and (i) tube circumference of the capillary tubes formed in vitro compared with controls. (j) PWS blood vessel-like phenotypes were observed in EphB1+/EfnB2+ but not in wild-type and EphB1+/EGFP+ control hDMVECs in an in vitro capillary tube formation assay at 12 h after cell plating. Positive stain is diaminobenzidine (DAB; brown). Scale bar = 100 μm. *P < 0·05, ** P < 0·01 vs. control. The arrows indicate blood vessels. (k) Left panel, detection of expression of EphB1, EphB4, β-actin and EfnB2 by Western blot (W.B.) in various hDMVEC subpopulations. hDMVEC, heterogeneous population prior to EfnB2–Fc selection; EphB1− hDMVEC, the remaining hDMVEC subpopulation after EfnB2–Fc selection; enhanced green fluorescent protein (EGFP) or EfnB2–His, overexpression of EGFP or EfnB2 in the EfnB2–Fc selected hDMVEC subpopulation. Right panel, an anti-EphB1 antibody was used to immunoprecipitate EphB1 from cell lysate and EfnB2 was detected from the immunoprecipitated protein complex using an anti-EfnB2 or anti-His antibody.

    Article Snippet: Human dermal microvascular endothelial cells (hDMVECs) were purchased from Cell Applications (San Diego, CA, U.S.A.) and were cultured in EC Basal Medium with growth supplement (Cell Applications).

    Techniques: Staining, Expressing, In Vitro, Control, Capillary Tube Formation Assay, Western Blot, Selection, Over Expression, Immunoprecipitation

    Port-wine stain (PWS) endothelial cells (ECs) showed dual arterial and venous identities of co-expression of Eph receptor B1 (EphB1) and ephrin B2 (EfnB2). (a, b) PWS ECs were EphB1+ and EfnB2+. (c, d) A normal dermal venule showed expression of EphB1 but not EfnB2. (e) PWS ECs were EphB4−. (f) Relative mRNA levels of EphB1 and EfnB2 in selected normal human dermal microvascular endothelial cell (hDMVEC) subtypes. (g–i) Forced co-expression of EphB1 and EfnB2 in normal hDMVECs showed a significant increase in (g) branch thickness, (h) branch point area and (i) tube circumference of the capillary tubes formed in vitro compared with controls. (j) PWS blood vessel-like phenotypes were observed in EphB1+/EfnB2+ but not in wild-type and EphB1+/EGFP+ control hDMVECs in an in vitro capillary tube formation assay at 12 h after cell plating. Positive stain is diaminobenzidine (DAB; brown). Scale bar = 100 μm. *P < 0·05, ** P < 0·01 vs. control. The arrows indicate blood vessels. (k) Left panel, detection of expression of EphB1, EphB4, β-actin and EfnB2 by Western blot (W.B.) in various hDMVEC subpopulations. hDMVEC, heterogeneous population prior to EfnB2–Fc selection; EphB1− hDMVEC, the remaining hDMVEC subpopulation after EfnB2–Fc selection; enhanced green fluorescent protein (EGFP) or EfnB2–His, overexpression of EGFP or EfnB2 in the EfnB2–Fc selected hDMVEC subpopulation. Right panel, an anti-EphB1 antibody was used to immunoprecipitate EphB1 from cell lysate and EfnB2 was detected from the immunoprecipitated protein complex using an anti-EfnB2 or anti-His antibody.

    Journal: The British journal of dermatology

    Article Title: Coexistence of Eph receptor B 1 and ephrin B 2 in port-wine stain endothelial progenitor cells contributes to clinicopathological vasculature dilatation

    doi: 10.1111/bjd.15716

    Figure Lengend Snippet: Port-wine stain (PWS) endothelial cells (ECs) showed dual arterial and venous identities of co-expression of Eph receptor B1 (EphB1) and ephrin B2 (EfnB2). (a, b) PWS ECs were EphB1+ and EfnB2+. (c, d) A normal dermal venule showed expression of EphB1 but not EfnB2. (e) PWS ECs were EphB4−. (f) Relative mRNA levels of EphB1 and EfnB2 in selected normal human dermal microvascular endothelial cell (hDMVEC) subtypes. (g–i) Forced co-expression of EphB1 and EfnB2 in normal hDMVECs showed a significant increase in (g) branch thickness, (h) branch point area and (i) tube circumference of the capillary tubes formed in vitro compared with controls. (j) PWS blood vessel-like phenotypes were observed in EphB1+/EfnB2+ but not in wild-type and EphB1+/EGFP+ control hDMVECs in an in vitro capillary tube formation assay at 12 h after cell plating. Positive stain is diaminobenzidine (DAB; brown). Scale bar = 100 μm. *P < 0·05, ** P < 0·01 vs. control. The arrows indicate blood vessels. (k) Left panel, detection of expression of EphB1, EphB4, β-actin and EfnB2 by Western blot (W.B.) in various hDMVEC subpopulations. hDMVEC, heterogeneous population prior to EfnB2–Fc selection; EphB1− hDMVEC, the remaining hDMVEC subpopulation after EfnB2–Fc selection; enhanced green fluorescent protein (EGFP) or EfnB2–His, overexpression of EGFP or EfnB2 in the EfnB2–Fc selected hDMVEC subpopulation. Right panel, an anti-EphB1 antibody was used to immunoprecipitate EphB1 from cell lysate and EfnB2 was detected from the immunoprecipitated protein complex using an anti-EfnB2 or anti-His antibody.

    Article Snippet: Human dermal microvascular endothelial cell culture Human dermal microvascular endothelial cells (hDMVECs) were purchased from Cell Applications (San Diego, CA, U.S.A.) and were cultured in EC Basal Medium with growth supplement (Cell Applications).

    Techniques: Staining, Expressing, In Vitro, Control, Capillary Tube Formation Assay, Western Blot, Selection, Over Expression, Immunoprecipitation