c1 manual (fluidigm)
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C1 Manual, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 2081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+manual/C1/pm37344645-550-12-11
Average 96 stars, based on 2081 article reviews
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Single Cell:Article Title: Signalling by senescent melanocytes hyperactivates hair growth Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Article Title: Signalling by senescent melanocytes hyperactivates hair growth. Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the RNA Sequencing:Article Title: Signalling by senescent melanocytes hyperactivates hair growth Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Article Title: Signalling by senescent melanocytes hyperactivates hair growth. Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Construct:Article Title: Signalling by senescent melanocytes hyperactivates hair growth Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Article Title: Signalling by senescent melanocytes hyperactivates hair growth. Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Sequencing:Article Title: Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons Article Snippet: © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Fluidigm C1 manual (see INTRODUCTION and MATERIALS). .. © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Article Title: Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons Article Snippet: .. For procedural details for preparing sequencing libraries, we refer users to the Control:Article Title: Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons Article Snippet: © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Fluidigm C1 manual (see INTRODUCTION and MATERIALS). .. © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Next-Generation Sequencing:Article Title: Proliferation does not contribute to murine models of renin cell recruitment Article Snippet: The samples were quantified using a Qubit 3.0Fluorometer (Thermo Fisher Scientific, Waltham, MA). .. Samples underwent next-generation sequencing library preparation as described in the Article Title: Proliferation does not contribute to murine models of renin cell recruitment Article Snippet: The samples were quantified using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific). .. Samples underwent next‐generation sequencing library preparation as described in the |
