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c1 manual  (fluidigm)


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    Structured Review

    fluidigm c1 manual
    C1 Manual, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 2081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1+manual/C1/pm37344645-550-12-11
    Average 96 stars, based on 2081 article reviews
    c1 manual - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Single Cell:

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth
    Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual. .. Multiplexed libraries were analysed on Agilent 2100 Bioanalyzer for fragment distribution and quantified using Kapa Biosystem’s universal library quantification kit.

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth.
    Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual. .. Multiplexed libraries were analysed on Agilent 2100 Bioanalyzer for fragment distribution and quantified using Kapa Biosystem’s universal library quantification kit.

    RNA Sequencing:

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth
    Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual. .. Multiplexed libraries were analysed on Agilent 2100 Bioanalyzer for fragment distribution and quantified using Kapa Biosystem’s universal library quantification kit.

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth.
    Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual. .. Multiplexed libraries were analysed on Agilent 2100 Bioanalyzer for fragment distribution and quantified using Kapa Biosystem’s universal library quantification kit.

    Construct:

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth
    Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual. .. Multiplexed libraries were analysed on Agilent 2100 Bioanalyzer for fragment distribution and quantified using Kapa Biosystem’s universal library quantification kit.

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth.
    Article Snippet: After cell capture, chips were examined visually under the microscope to determine the capture rate, and empty chambers or chambers with multiple cells were excluded from the analysis. cDNA was synthesized and amplified on the Fluidigm C1 Single-Cell Auto Prep System with the Clontech SMARTer Ultra Low RNA kit and the ADVANTAGE-2 PCR kit (Clontech). .. Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual. .. Multiplexed libraries were analysed on Agilent 2100 Bioanalyzer for fragment distribution and quantified using Kapa Biosystem’s universal library quantification kit.

    Sequencing:

    Article Title: Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons
    Article Snippet: © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Fluidigm C1 manual (see INTRODUCTION and MATERIALS). .. © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Fluidigm C1 manual (see INTRODUCTION and MATERIALS). .. Determine the quality of the final pooled Nextera XT libraries, for example, by using the high-sensitivity DNA kit for Agilent Bioanalyzer according to the manufacturer’s recommendations.

    Article Title: Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons
    Article Snippet: .. For procedural details for preparing sequencing libraries, we refer users to the Fluidigm C1 manual (C1 System for mRNA-Seq, part no. 100-7168 available at https://www.fluidigm.com/documents; select ‘C1 System for mRNA Seq’ to download the PDF automatically). .. We use the Illumina Nextera XT library preparation kit and perform multiplexed paired-end sequencing of barcoded libraries using an Illumina MiSeq system.

    Control:

    Article Title: Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons
    Article Snippet: © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Fluidigm C1 manual (see INTRODUCTION and MATERIALS). .. © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 514 | VOL.11 NO.3 | 2016 | nature protocols sequencing library preparation ● tIMInG 2 h 24| Use cDNA preparations (from Step 19) that pass quality control (Step 23) to prepare a sequencing library; we use the Illumina Nextera XT library prep kit and follow the instructions in the Fluidigm C1 manual (see INTRODUCTION and MATERIALS). .. Determine the quality of the final pooled Nextera XT libraries, for example, by using the high-sensitivity DNA kit for Agilent Bioanalyzer according to the manufacturer’s recommendations.

    Next-Generation Sequencing:

    Article Title: Proliferation does not contribute to murine models of renin cell recruitment
    Article Snippet: The samples were quantified using a Qubit 3.0Fluorometer (Thermo Fisher Scientific, Waltham, MA). .. Samples underwent next-generation sequencing library preparation as described in the Fluidigm C1 manual using an Illumina NexteraXT library prep and index kits (Catalog Nos. ..

    Article Title: Proliferation does not contribute to murine models of renin cell recruitment
    Article Snippet: The samples were quantified using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific). .. Samples underwent next‐generation sequencing library preparation as described in the Fluidigm C1 manual using an Illumina Nextera XT library prep and index kits (Catalog Nos. ..



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    Fig. 2 | Hair SCs within nevus skin lose quiescence. a, <t>On</t> <t>RNA-seq</t> analysis, Tyr-NrasQ61K bulge SCs differ from P30 and P56 WT bulge SCs. A principal component analysis plot is shown. See Extended Data Fig. 6. b, A list of selected downregulated (red) and upregulated (green) genes at P56 and Tyr-NrasQ61K to WT fold change values. c, qRT–PCR of selected differentially expressed genes from a. n = 3. d, t-Distributed stochastic neighbour embedding (t-SNE) analysis on single-cell RNA-seq data for P30 and P56 WT and P56 Tyr-NrasQ61K bulge SCs. Cells form five clusters: <t>C1</t> to C5. e, Cladogram showing relative cluster similarity. f, t-SNE plot colour-coded by sample source. g, t-SNE plot colour- coded by inferred cell cycle state. h, Violin plots for selected genes. See Extended Data Fig. 6. TPM, transcripts per million. i, EdU pulse-chase analysis on bulge SCs. Unlike total numbers of CD34+CD49f+ bulge SCs (top), their EdU+
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    Fig. 2 | Hair SCs within nevus skin lose quiescence. a, On RNA-seq analysis, Tyr-NrasQ61K bulge SCs differ from P30 and P56 WT bulge SCs. A principal component analysis plot is shown. See Extended Data Fig. 6. b, A list of selected downregulated (red) and upregulated (green) genes at P56 and Tyr-NrasQ61K to WT fold change values. c, qRT–PCR of selected differentially expressed genes from a. n = 3. d, t-Distributed stochastic neighbour embedding (t-SNE) analysis on single-cell RNA-seq data for P30 and P56 WT and P56 Tyr-NrasQ61K bulge SCs. Cells form five clusters: C1 to C5. e, Cladogram showing relative cluster similarity. f, t-SNE plot colour-coded by sample source. g, t-SNE plot colour- coded by inferred cell cycle state. h, Violin plots for selected genes. See Extended Data Fig. 6. TPM, transcripts per million. i, EdU pulse-chase analysis on bulge SCs. Unlike total numbers of CD34+CD49f+ bulge SCs (top), their EdU+

    Journal: Nature

    Article Title: Signalling by senescent melanocytes hyperactivates hair growth.

    doi: 10.1038/s41586-023-06172-8

    Figure Lengend Snippet: Fig. 2 | Hair SCs within nevus skin lose quiescence. a, On RNA-seq analysis, Tyr-NrasQ61K bulge SCs differ from P30 and P56 WT bulge SCs. A principal component analysis plot is shown. See Extended Data Fig. 6. b, A list of selected downregulated (red) and upregulated (green) genes at P56 and Tyr-NrasQ61K to WT fold change values. c, qRT–PCR of selected differentially expressed genes from a. n = 3. d, t-Distributed stochastic neighbour embedding (t-SNE) analysis on single-cell RNA-seq data for P30 and P56 WT and P56 Tyr-NrasQ61K bulge SCs. Cells form five clusters: C1 to C5. e, Cladogram showing relative cluster similarity. f, t-SNE plot colour-coded by sample source. g, t-SNE plot colour- coded by inferred cell cycle state. h, Violin plots for selected genes. See Extended Data Fig. 6. TPM, transcripts per million. i, EdU pulse-chase analysis on bulge SCs. Unlike total numbers of CD34+CD49f+ bulge SCs (top), their EdU+

    Article Snippet: Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual.

    Techniques: RNA Sequencing, Quantitative RT-PCR, Pulse Chase