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Biacore planar c1 chip
Planar C1 Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+chip/667+c1+chip+sensor/pmc11949114-55-10-1
Average 86 stars, based on 1 article reviews
planar c1 chip - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Injection:

Article Title: Anti-CD33 antibodies and methods of use thereof
Article Snippet: BIAcore measurements were performed as follows: human CD33 was immobilized on the surface of a C1 chip, and Fabs were injected for 300 s to monitor association, followed by a 900 s flow in running buffer to monitor dissociation. .. BIAcore measurements were performed as follows: human CD33 was immobilized on the surface of a C1 chip, and Fabs were injected for 300 s to monitor association, followed by a 900 s flow in running buffer to monitor dissociation. ..

SPR Assay:

Article Title: A new enzyme-linked immunosorbent assay for neurofilament light in cerebrospinal fluid: analytical validation and clinical evaluation.
Article Snippet: Finally, antibodies were purified using a protein G column (GE Healthcare). .. Surface plasmon resonance and immunoprecipitation/ Western blot analyses Anti-mouse antibody (mouse antibody capture kit BR-1008-38; GE Healthcare) was immobilized according to the kit instructions on a C1 Biacore chip (BR-100540) in a Biacore X100 instrument via amine-coupling at 25 °C in running buffer (PBS with 5% DMSO and 0.05% Tween 20). ..

Article Title: Insight into the avidity–affinity relationship of the bivalent, pH-dependent interaction between IgG and FcRn
Article Snippet: .. For a direct comparison with Biacore SPR technology, we captured low (12 RU) and medium FcRn (140 RU) levels on a C1 chip, allowing affinity (low) and affinity and avidity (medium) to occur simultaneously. ..

Immunoprecipitation:

Article Title: A new enzyme-linked immunosorbent assay for neurofilament light in cerebrospinal fluid: analytical validation and clinical evaluation.
Article Snippet: Finally, antibodies were purified using a protein G column (GE Healthcare). .. Surface plasmon resonance and immunoprecipitation/ Western blot analyses Anti-mouse antibody (mouse antibody capture kit BR-1008-38; GE Healthcare) was immobilized according to the kit instructions on a C1 Biacore chip (BR-100540) in a Biacore X100 instrument via amine-coupling at 25 °C in running buffer (PBS with 5% DMSO and 0.05% Tween 20). ..

Western Blot:

Article Title: A new enzyme-linked immunosorbent assay for neurofilament light in cerebrospinal fluid: analytical validation and clinical evaluation.
Article Snippet: Finally, antibodies were purified using a protein G column (GE Healthcare). .. Surface plasmon resonance and immunoprecipitation/ Western blot analyses Anti-mouse antibody (mouse antibody capture kit BR-1008-38; GE Healthcare) was immobilized according to the kit instructions on a C1 Biacore chip (BR-100540) in a Biacore X100 instrument via amine-coupling at 25 °C in running buffer (PBS with 5% DMSO and 0.05% Tween 20). ..

Protein Binding:

Article Title: Deriving a sub-nanomolar affinity peptide from TAP to enable smFRET analysis of RNA polymerase II complexes.
Article Snippet: Our capability to visualize protein complexes such as RNA polymerase II (pol II) by single-molecule imaging techniques has largely been hampered by the absence of a simple bio-orthogonal approach for selective labeling with a fluorescent probe.. Here, we modified the existing calmodulin-binding peptide (CBP) in the widely used Tandem Affinity Purification (TAP) tag to endow it with a high affinity for calmodulin (CaM) and used dye-CaM to conduct site-specific labeling of pol II.. To demonstrate the single molecule applicability of this approach, we labeled the C-terminus of the Rpb9 subunit of pol II with donor-CaM and a site in TFIIF with an acceptor to generate a FRET (fluorescence resonance energy transfer) pair in the pol II-TFIIF complex.

Transferring:

Article Title: The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity
Article Snippet: .. By transferring the assay from ProteOn's GLM chip to Biacore's C1 chip, we diluted out avidity artifacts and revealed that the hIgG1 homodimers bound to sparsely immobilized FcRn with homogeneous responses ( Fig. S2A ) that were kinetically indistinguishable from those of their corresponding monovalent heterodimers ( Fig. S2B ). ..

Comparison:

Article Title: Insight into the avidity–affinity relationship of the bivalent, pH-dependent interaction between IgG and FcRn
Article Snippet: .. For a direct comparison with Biacore SPR technology, we captured low (12 RU) and medium FcRn (140 RU) levels on a C1 chip, allowing affinity (low) and affinity and avidity (medium) to occur simultaneously. ..



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