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gentlemacs c tube  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec gentlemacs c tube
    Gentlemacs C Tube, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+kit/Multi+Tissue+Dissociation+Kit+1/pm42533102-336-5-8
    Average 96 stars, based on 256 article reviews
    gentlemacs c tube - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Derivative Assay:

    Article Title: Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors
    Article Snippet: The isolated aortic adventitia was then cut into small pieces, seeded in a 0.04% gelatin (Sigma, G1393)-coated T25 flask, and maintained in complete stem cell culture medium, which consists of DMEM (ATCC, 30-2002), 10% EmbryoMax ES Cell Qualified FBS (Millipore, ES-009-B), 10-ng/mL leukemia inhibitory factor (Merck Millipore, LIF1050), 0.1-mM 2-mercaptoethanol (Gibco, 31350–010), 100 U/mL penicillin–streptomycin (Gibco, 15140122), and 2-mM L-glutamine (Gibco, 25030081). .. Cells derived from the outgrowth of aortic adventitia were then passaged for cell enrichment. c-Kit + cells were then sorted from these enriched cells using c-Kit (Miltenyi Biotec, 130-091-224) microbeads according to the manufacturer’s instructions. ..

    Article Title: Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors.
    Article Snippet: Stem/progenitor cells (SPCs) have been implicated to participate in vascular repair.. However, the exact role of SPCs in endothelial repair of large vessels still remains controversial.. This study aimed to delineate the cellular heterogeneity and possible functional role of endogenous vascular SPCs in large vessels.

    Cell Isolation:

    Article Title: Assessment of non-myelotoxic agents as a preparatory regimen for hematopoietic stem cell gene therapy.
    Article Snippet: RAG2 deficiency is characterized by a lack of B and T lymphocytes, causing severe lethal infections.. Currently, RAG2 deficiency is treated with a Hematopoietic Stem Cell transplantation (HSCT).. Most conditioning regimens used before HSCT consist of alkylating myelotoxic agents with or without irradiation and affect growth and development of pediatric patients.

    Article Title: Non-Myelotoxic Agents as a Preparatory Regimen for Hematopoietic Stem Cell Gene Therapy
    Article Snippet: .. Hematopoietic Stem Cell isolation and characterization BM from male Rag2-/- mice was collected and lineage depletion was performed using a Lineage Depletion kit (Miltenyi Biotech, #130-090-858), followed by positive selection for c-Kit (Miltenyi Biotec, #130-091-224). .. The c-Kit+ positive fraction was labeled using anti-c-Kit antibody 5 (BioLegend, #105812) and anti-Sca-1 antibody (BioLegend, #108116), measured using a BD AccuriTM C6 Plus (Becton Dickinson) and analyzed using the BD CSampler software.

    Selection:

    Article Title: Assessment of non-myelotoxic agents as a preparatory regimen for hematopoietic stem cell gene therapy.
    Article Snippet: RAG2 deficiency is characterized by a lack of B and T lymphocytes, causing severe lethal infections.. Currently, RAG2 deficiency is treated with a Hematopoietic Stem Cell transplantation (HSCT).. Most conditioning regimens used before HSCT consist of alkylating myelotoxic agents with or without irradiation and affect growth and development of pediatric patients.

    Article Title: Non-Myelotoxic Agents as a Preparatory Regimen for Hematopoietic Stem Cell Gene Therapy
    Article Snippet: .. Hematopoietic Stem Cell isolation and characterization BM from male Rag2-/- mice was collected and lineage depletion was performed using a Lineage Depletion kit (Miltenyi Biotech, #130-090-858), followed by positive selection for c-Kit (Miltenyi Biotec, #130-091-224). .. The c-Kit+ positive fraction was labeled using anti-c-Kit antibody 5 (BioLegend, #105812) and anti-Sca-1 antibody (BioLegend, #108116), measured using a BD AccuriTM C6 Plus (Becton Dickinson) and analyzed using the BD CSampler software.

    Staining:

    Article Title: Blimp-1 integrates alarmin signals in ILC2s and drives proinflammatory functions required for type 2 immunity
    Article Snippet: .. For surface staining, the following antibodies were used: CD101 (REA301, Miltenyi), c-Kit (2B8), CD11b (M1/70), CD11c (N418), IL-25R (Munc33), CD127 (A7R34), CD19 (1D3), CD4 (GK1.5 and RM4-5), CD45 (30-F11) or CD45.2 (104), CD90 (30H12), F4/80 (BM8), KLRG1 (2F1 or MAFA), NK1.1 (PK136), PD-1 (29F.1A12), Sca-1 (D7), Siglec F (E50-2440), ST2 (RMST2-33), and TSLPR (22H9). ..

    Article Title: Blimp-1 integrates alarmin signals in ILC2s and drives proinflammatory functions required for type 2 immunity.
    Article Snippet: .. For surface staining, the following antibodies were used: CD101 (REA301, Miltenyi), c-Kit (2B8), CD11b (M1/70), CD11c (N418), IL25R (Munc33), CD127 (A7R34), CD19 (1D3), CD4 (GK1.5 and RM4-5), CD45 (30-F11) or CD45.2 (104), CD90 (30H12), F4/80 (BM8), KLRG1 (2F1 or MAFA), NK1.1 (PK136), PD-1 (29F.1A12), Sca-1 (D7), Siglec F (E50-2440), ST2 (RMST2-33), and TSLPR (22H9). ..

    other:

    Article Title: Facilitative lysosomal transport of bile acids alleviates ER stress in mouse hematopoietic precursors
    Article Snippet: Antibody conjugates for Flt3 (PE-CF594-anti-CD135, clone A2F10.1; 1:50 dilution), IL7Ra (V450-anti-CD127, clone SB/199; 1:50 dilution), Sca1 (PE-Cy7-anti-Ly-6A/E, clone D7; 1:50 dilution), and CD34 (FITC-anti-CD34, clone RAM34; 1:50 dilution) were obtained from BD Biosciences (San Jose, CA), while c-kit (PE-anti-CD117, clone REA791; 1:50 dilution) was obtained from Miltenyi Biotech (Bergisch Galadbach, Germany).



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    Image Search Results


    Effect of combinatorial therapies on blood plasma parameters. Blood plasma from female ( A , C , E ) and male ( B , D , F ) mice was analyzed for AST activity ( A , B ), creatinine concentrations ( C , D ), and C-peptide levels ( E , F ) following treatment with the BI-3406 (BI) and trametinib (T) combination therapy with or without BKM120 (BKM). Shaded areas indicate the range observed in healthy C57BL/6 mice (n = 5). Statistical analyses were performed using an unpaired t-test ( C – E ) or Mann–Whitney test ( A , B , F ). *p < 0.05

    Journal: BMC Cancer

    Article Title: Benefits and challenges of adding BKM120 to a BI-3406 plus trametinib combination therapy

    doi: 10.1186/s12885-026-16409-0

    Figure Lengend Snippet: Effect of combinatorial therapies on blood plasma parameters. Blood plasma from female ( A , C , E ) and male ( B , D , F ) mice was analyzed for AST activity ( A , B ), creatinine concentrations ( C , D ), and C-peptide levels ( E , F ) following treatment with the BI-3406 (BI) and trametinib (T) combination therapy with or without BKM120 (BKM). Shaded areas indicate the range observed in healthy C57BL/6 mice (n = 5). Statistical analyses were performed using an unpaired t-test ( C – E ) or Mann–Whitney test ( A , B , F ). *p < 0.05

    Article Snippet: Blood chemistry parameters, such as aspartate aminotransferase (AST), and creatinine were measured using a cobas c111 system (Roche Diagnostics, Mannheim, Germany), and C-peptide levels were quantified with a mouse C-peptide ELISA kit (ALPCO, Salem, USA) according to the manufacturer’s instructions.

    Techniques: Clinical Proteomics, Activity Assay, MANN-WHITNEY