gentlemacs c tube (Miltenyi Biotec)
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Gentlemacs C Tube, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+kit/Multi+Tissue+Dissociation+Kit+1/pm42533102-336-5-8
Average 96 stars, based on 256 article reviews
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Derivative Assay:Article Title: Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors Article Snippet: The isolated aortic adventitia was then cut into small pieces, seeded in a 0.04% gelatin (Sigma, G1393)-coated T25 flask, and maintained in complete stem cell culture medium, which consists of DMEM (ATCC, 30-2002), 10% EmbryoMax ES Cell Qualified FBS (Millipore, ES-009-B), 10-ng/mL leukemia inhibitory factor (Merck Millipore, LIF1050), 0.1-mM 2-mercaptoethanol (Gibco, 31350–010), 100 U/mL penicillin–streptomycin (Gibco, 15140122), and 2-mM L-glutamine (Gibco, 25030081). .. Cells derived from the outgrowth of aortic adventitia were then passaged for cell enrichment. Article Title: Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors. Article Snippet: Stem/progenitor cells (SPCs) have been implicated to participate in vascular repair.. However, the exact role of SPCs in endothelial repair of large vessels still remains controversial.. This study aimed to delineate the cellular heterogeneity and possible functional role of endogenous vascular SPCs in large vessels. Cell Isolation:Article Title: Assessment of non-myelotoxic agents as a preparatory regimen for hematopoietic stem cell gene therapy. Article Snippet: RAG2 deficiency is characterized by a lack of B and T lymphocytes, causing severe lethal infections.. Currently, RAG2 deficiency is treated with a Hematopoietic Stem Cell transplantation (HSCT).. Most conditioning regimens used before HSCT consist of alkylating myelotoxic agents with or without irradiation and affect growth and development of pediatric patients. Article Title: Non-Myelotoxic Agents as a Preparatory Regimen for Hematopoietic Stem Cell Gene Therapy Article Snippet: .. Hematopoietic Stem Cell isolation and characterization BM from male Rag2-/- mice was collected and lineage depletion was performed using a Lineage Depletion kit (Miltenyi Biotech, #130-090-858), followed by positive selection for Selection:Article Title: Assessment of non-myelotoxic agents as a preparatory regimen for hematopoietic stem cell gene therapy. Article Snippet: RAG2 deficiency is characterized by a lack of B and T lymphocytes, causing severe lethal infections.. Currently, RAG2 deficiency is treated with a Hematopoietic Stem Cell transplantation (HSCT).. Most conditioning regimens used before HSCT consist of alkylating myelotoxic agents with or without irradiation and affect growth and development of pediatric patients. Article Title: Non-Myelotoxic Agents as a Preparatory Regimen for Hematopoietic Stem Cell Gene Therapy Article Snippet: .. Hematopoietic Stem Cell isolation and characterization BM from male Rag2-/- mice was collected and lineage depletion was performed using a Lineage Depletion kit (Miltenyi Biotech, #130-090-858), followed by positive selection for Staining:Article Title: Blimp-1 integrates alarmin signals in ILC2s and drives proinflammatory functions required for type 2 immunity Article Snippet: .. For surface staining, the following antibodies were used: CD101 (REA301, Miltenyi), Article Title: Blimp-1 integrates alarmin signals in ILC2s and drives proinflammatory functions required for type 2 immunity. Article Snippet: .. For surface staining, the following antibodies were used: CD101 (REA301, Miltenyi), other:Article Title: Facilitative lysosomal transport of bile acids alleviates ER stress in mouse hematopoietic precursors Article Snippet: Antibody conjugates for Flt3 (PE-CF594-anti-CD135, clone A2F10.1; 1:50 dilution), IL7Ra (V450-anti-CD127, clone SB/199; 1:50 dilution), Sca1 (PE-Cy7-anti-Ly-6A/E, clone D7; 1:50 dilution), and CD34 (FITC-anti-CD34, clone RAM34; 1:50 dilution) were obtained from BD Biosciences (San Jose, CA), while c-kit ( |
