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c33a  (ATCC)


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    ATCC c33a
    C33a, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+33+a/C-33+A/pm42301387-37-11-18
    Average 96 stars, based on 1138 article reviews
    c33a - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: EIF3B‑METTL3 complex promotes cell proliferation, invasion and EGFR/AKT signaling in cervical cancer.
    Article Snippet: .. Human cervical epithelial cells (HCerEpic; cat. no. 7060, ScienCell Research Laboratories, Inc.), as well as C‐33 A (cat. no. HTB‐31), HeLa (cat. no. CCL‐2), SiHa (cat. no. HTB‐35) and CaSki [cat. no. CRL‐1550; all from American Type Culture Collection (ATCC)] cells were cultured in cervical epithelial cell medium, which contains basal medium and Cervical Epithelial Cell Growth Supplement (cat. no. 7061; ScienCell Research Laboratories, Inc.) or Eagle's minimum essential medium with Earle's salts, L‐glutamine, and non‐essential amino acids, without sodium bicarbonate (cat. no. M0643; MilliporeSigma). .. The medium was supple‐ mented with 10% fetal bovine serum (FBS; cat. no. ABS972; Absin Bioscience Inc.).

    Article Title: The cell-surface serine protease prostasin is lost during cervical squamous cell carcinogenesis.
    Article Snippet: The glycosylphosphatidylinositol (GPI)-anchored serine protease prostasin has been reported to have increased expression with tumor-promoting properties in some cancer types, while expression is lost and prostasin displays tumor-suppressing properties in other cancer types.. Due to these context-dependent and opposing expression patterns and functions of prostasin, characterization of each cancer type is important.. In the present study, we aimed to determine the expression of prostasin in the normal cervix and in cervical squamous cell carcinoma (CSCC), the most common type of cervical cancer.

    Article Title: EIF3B-METTL3 complex promotes cell proliferation, invasion and EGFR/AKT signaling in cervical cancer
    Article Snippet: .. Human cervical epithelial cells (HCerEpic; cat. no. 7060, ScienCell Research Laboratories, Inc.), as well as C-33 A (cat. no. HTB-31), HeLa (cat. no. CCL-2), SiHa (cat. no. HTB-35) and CaSki [cat. no. CRL-1550; all from American Type Culture Collection (ATCC)] cells were cultured in cervical epithelial cell medium, which contains basal medium and Cervical Epithelial Cell Growth Supplement (cat. no. 7061; ScienCell Research Laboratories, Inc.) or Eagle's minimum essential medium with Earle's salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate (cat. no. M0643; MilliporeSigma). .. The medium was supplemented with 10% fetal bovine serum (FBS; cat. no. ABS972; Absin Bioscience Inc.).

    Article Title: The cell-surface serine protease prostasin is lost during cervical squamous cell carcinogenesis
    Article Snippet: For detection, goat anti-rabbit (12-348, Millipore Sigma, Burlington, MA) or goat anti-mouse (AP181P, Millipore Sigma, Burlington, MA) HRP-linked secondary antibodies (Millipore Sigma, Burlington, MA) were used in combination with SuperSignal West Femto (Thermo Scientific, Waltham, MA) or Clarity Western ECL Substrate (Biorad, Hercules, CA). .. Human cervical carcinoma cells, C-33 A [ 10 ] (ATCC, Manassas, VA), were cultured in Dulbecco’s modified Eagle’s media (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 10 units/mL Penicillin, and 10μg/mL streptomycin (Gibco, Life Technologies, Grand Island, NY). .. Human breast epithelial cells, MCF10A [ 11 ] (ATCC, Manassas, VA), were cultured in Dulbecco’s modified Eagle media and Ham’s F12 (DMEM/F12, Gibco, Life Technologies, Grand Island, NY) supplemented with 5% Horse serum (Gibco, Life Technologies, Grand Island, NY), 10mM HEPES (Millipore Sigma, Burlington, MA), 1μg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO), 1X Insulin-Transferrin-Selenium (ThermoFisher, Waltham, MA), 10ng/mL EGF (Millipore Sigma, Burlington, MA), and 10 units/mL Penicillin, and 10μg/mL streptomycin (Gibco, Life Technologies, Grand Island, NY).

    Modification:

    Article Title: The cell-surface serine protease prostasin is lost during cervical squamous cell carcinogenesis.
    Article Snippet: The glycosylphosphatidylinositol (GPI)-anchored serine protease prostasin has been reported to have increased expression with tumor-promoting properties in some cancer types, while expression is lost and prostasin displays tumor-suppressing properties in other cancer types.. Due to these context-dependent and opposing expression patterns and functions of prostasin, characterization of each cancer type is important.. In the present study, we aimed to determine the expression of prostasin in the normal cervix and in cervical squamous cell carcinoma (CSCC), the most common type of cervical cancer.

    Article Title: The cell-surface serine protease prostasin is lost during cervical squamous cell carcinogenesis
    Article Snippet: For detection, goat anti-rabbit (12-348, Millipore Sigma, Burlington, MA) or goat anti-mouse (AP181P, Millipore Sigma, Burlington, MA) HRP-linked secondary antibodies (Millipore Sigma, Burlington, MA) were used in combination with SuperSignal West Femto (Thermo Scientific, Waltham, MA) or Clarity Western ECL Substrate (Biorad, Hercules, CA). .. Human cervical carcinoma cells, C-33 A [ 10 ] (ATCC, Manassas, VA), were cultured in Dulbecco’s modified Eagle’s media (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 10 units/mL Penicillin, and 10μg/mL streptomycin (Gibco, Life Technologies, Grand Island, NY). .. Human breast epithelial cells, MCF10A [ 11 ] (ATCC, Manassas, VA), were cultured in Dulbecco’s modified Eagle media and Ham’s F12 (DMEM/F12, Gibco, Life Technologies, Grand Island, NY) supplemented with 5% Horse serum (Gibco, Life Technologies, Grand Island, NY), 10mM HEPES (Millipore Sigma, Burlington, MA), 1μg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO), 1X Insulin-Transferrin-Selenium (ThermoFisher, Waltham, MA), 10ng/mL EGF (Millipore Sigma, Burlington, MA), and 10 units/mL Penicillin, and 10μg/mL streptomycin (Gibco, Life Technologies, Grand Island, NY).



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    Image Search Results


    Dose-dependent effect of resveratrol (RES) on cellular metabolic activity in cancer cell lines with different p53 status. Cells were treated with increasing concentrations of RES (50–250 µM) for 48 h. Cellular metabolic activity was assessed by means of MTT assay and normalized to that of vehicle-treated controls (EtOH 0.3% v / v ). Data represent mean ± SD of at least four independent experiments. IC50 values are indicated for each cell line (presented in box).

    Journal: International Journal of Molecular Sciences

    Article Title: Reactivation of P53 Antiproliferative and Pro-Apoptotic Pathways by Resveratrol in Mutant P53 Cancer Cell Lines

    doi: 10.3390/ijms27104481

    Figure Lengend Snippet: Dose-dependent effect of resveratrol (RES) on cellular metabolic activity in cancer cell lines with different p53 status. Cells were treated with increasing concentrations of RES (50–250 µM) for 48 h. Cellular metabolic activity was assessed by means of MTT assay and normalized to that of vehicle-treated controls (EtOH 0.3% v / v ). Data represent mean ± SD of at least four independent experiments. IC50 values are indicated for each cell line (presented in box).

    Article Snippet: The human cervical cancer cell line C33A ( p53 mutation R273C) was purchased from the American Type Culture Collection (ATCC cat. no. HTB-31 [C-33A]; Manassas, VA, USA).

    Techniques: Activity Assay, MTT Assay

    Resveratrol induces apoptosis in cancer cell lines with mutant or null p53 . ( A ) C33A, SK-BR-3, and Saos-2 cells were treated with vehicle (ethanol) or resveratrol at their respective IC50s for 48 h. Apoptosis was assessed by means of Annexin V/propidium iodide (PI) double staining followed by flow cytometry analysis. Representative dot plots show viable cells in the lower left quadrant (Annexin V − /PI − ), early apoptotic cells in the lower right quadrant (Annexin V + /PI − ), and late apoptotic cells in the upper right quadrant (Annexin V + /PI + ). ( B ) Quantifications of total apoptotic cells (early + late apoptosis) are shown as the mean ± SD from three independent experiments. ** p < 0.01; *** p < 0.001 versus EtOH. RES, resveratrol; EtOH, control with ethanol.

    Journal: International Journal of Molecular Sciences

    Article Title: Reactivation of P53 Antiproliferative and Pro-Apoptotic Pathways by Resveratrol in Mutant P53 Cancer Cell Lines

    doi: 10.3390/ijms27104481

    Figure Lengend Snippet: Resveratrol induces apoptosis in cancer cell lines with mutant or null p53 . ( A ) C33A, SK-BR-3, and Saos-2 cells were treated with vehicle (ethanol) or resveratrol at their respective IC50s for 48 h. Apoptosis was assessed by means of Annexin V/propidium iodide (PI) double staining followed by flow cytometry analysis. Representative dot plots show viable cells in the lower left quadrant (Annexin V − /PI − ), early apoptotic cells in the lower right quadrant (Annexin V + /PI − ), and late apoptotic cells in the upper right quadrant (Annexin V + /PI + ). ( B ) Quantifications of total apoptotic cells (early + late apoptosis) are shown as the mean ± SD from three independent experiments. ** p < 0.01; *** p < 0.001 versus EtOH. RES, resveratrol; EtOH, control with ethanol.

    Article Snippet: The human cervical cancer cell line C33A ( p53 mutation R273C) was purchased from the American Type Culture Collection (ATCC cat. no. HTB-31 [C-33A]; Manassas, VA, USA).

    Techniques: Mutagenesis, Double Staining, Flow Cytometry, Control

    Resveratrol induces the expression of p53 target genes across different p53 backgrounds. Gene expression analysis was performed in ( A ) C33A ( p53 R273C), ( B ) SK-BR-3 ( p53 R175H), ( C ) MCF-7 ( p53 WT), and ( D ) Saos-2 ( p53 null) cells following treatment with vehicle (EtOH) or resveratrol (RES) at their respective IC50s for 48 h. Relative mRNA levels of p53 target genes were determined by means of RT-qPCR and expressed as fold changes relative to vehicle-treated controls, normalized to the housekeeping gene β2-microglobulin (β2M). Data are presented as the mean ± SD of at least three independent experiments. Statistical significance was evaluated using one-way ANOVA followed by appropriate post hoc multiple comparison tests. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 versus control.

    Journal: International Journal of Molecular Sciences

    Article Title: Reactivation of P53 Antiproliferative and Pro-Apoptotic Pathways by Resveratrol in Mutant P53 Cancer Cell Lines

    doi: 10.3390/ijms27104481

    Figure Lengend Snippet: Resveratrol induces the expression of p53 target genes across different p53 backgrounds. Gene expression analysis was performed in ( A ) C33A ( p53 R273C), ( B ) SK-BR-3 ( p53 R175H), ( C ) MCF-7 ( p53 WT), and ( D ) Saos-2 ( p53 null) cells following treatment with vehicle (EtOH) or resveratrol (RES) at their respective IC50s for 48 h. Relative mRNA levels of p53 target genes were determined by means of RT-qPCR and expressed as fold changes relative to vehicle-treated controls, normalized to the housekeeping gene β2-microglobulin (β2M). Data are presented as the mean ± SD of at least three independent experiments. Statistical significance was evaluated using one-way ANOVA followed by appropriate post hoc multiple comparison tests. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 versus control.

    Article Snippet: The human cervical cancer cell line C33A ( p53 mutation R273C) was purchased from the American Type Culture Collection (ATCC cat. no. HTB-31 [C-33A]; Manassas, VA, USA).

    Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Comparison, Control

    Resveratrol enhances cisplatin sensitivity in cancer cell lines with mutant or null p53 . ( A ) C33A, ( B ) SK-BR-3, and ( C ) Saos-2 cells were treated with increasing concentrations of cisplatin (CDDP; 2.5–20 μM) in the presence or absence of resveratrol (RES), at the corresponding IC 50 for each cell line, for 48 h. Cellular metabolic activity was assessed via MTT assay and normalized to that of vehicle-treated controls. Data represent the mean ± SD of at least four independent experiments. ( D ) IC50 values for CDDP were calculated using non-linear regression analysis (log [CDDP] vs. normalized response, variable slope model) using GraphPad Prism 6.01. * Previously published results from our working group.

    Journal: International Journal of Molecular Sciences

    Article Title: Reactivation of P53 Antiproliferative and Pro-Apoptotic Pathways by Resveratrol in Mutant P53 Cancer Cell Lines

    doi: 10.3390/ijms27104481

    Figure Lengend Snippet: Resveratrol enhances cisplatin sensitivity in cancer cell lines with mutant or null p53 . ( A ) C33A, ( B ) SK-BR-3, and ( C ) Saos-2 cells were treated with increasing concentrations of cisplatin (CDDP; 2.5–20 μM) in the presence or absence of resveratrol (RES), at the corresponding IC 50 for each cell line, for 48 h. Cellular metabolic activity was assessed via MTT assay and normalized to that of vehicle-treated controls. Data represent the mean ± SD of at least four independent experiments. ( D ) IC50 values for CDDP were calculated using non-linear regression analysis (log [CDDP] vs. normalized response, variable slope model) using GraphPad Prism 6.01. * Previously published results from our working group.

    Article Snippet: The human cervical cancer cell line C33A ( p53 mutation R273C) was purchased from the American Type Culture Collection (ATCC cat. no. HTB-31 [C-33A]; Manassas, VA, USA).

    Techniques: Mutagenesis, Activity Assay, MTT Assay