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cell lines bxpc 3  (ATCC)


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    ATCC cell lines bxpc 3
    Cell Lines Bxpc 3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bxpc+3+cells/BxPC-3/us12653850-1944-0-12
    Average 99 stars, based on 4841 article reviews
    cell lines bxpc 3 - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: Metabolic and Anti-Proliferative Responses of Pancreatic Cancer Cells to Ultrasound and Nanobubble Treatment
    Article Snippet: Cells were maintained at 37 °C in a humidified incubator with 5% CO 2 and passaged at a 1:5 ratio using 0.025% Trypsin-EDTA (Thermo Fisher Scientific, cat# 15090046). .. BxPC-3 cells (ATCC CRL-1687) were maintained in RPMI 1640 (GibcoTM, Thermo Fisher Scientific, Milano, cat# 11875093) supplemented with 10% (v/v) FCS (GibcoTM, Thermo Fisher Scientific, Milano, cat# 10437-036) under identical incubation conditions. ..

    Cell Culture:

    Article Title: Boosting the Activity of Melanoma-Targeting CAR-T Cells in the Presence of Citrate by the Application of Gluconate
    Article Snippet: T cells were cultured in RPMI 1640 medium, 1% ( w / v ) GlutaMAX (Gibco, ThermoFisher, Waltham, MA, USA), 100 IU/mL penicillin, 100 μg/mL streptomycin (Pan-Biotech, Aidenbach, Germany), 2 mM HEPES (PAA, Palo Alto, CA, USA), and 10% ( v / v ) heat-inactivated fetal calf serum (FCS) (Pan-Biotech, Aidenbach, Germany). .. BxPC-3 cells (ATCC CRL-1420), 293T cells (ATCC CRL-3216 American Type Culture Collection, Manassas, VA, USA), A375 cells, A375M cells, and IGR39 cells were cultured in DMEM, 1% ( w / v ) GlutaMAX (Gibco, ThermoFisher), 100 IU/mL penicillin, 100 μg/mL streptomycin (Pan-Biotech), and 10% ( v / v ) heat-inactivated FCS (Sigma-Aldrich, St. Louis, MO, USA). .. Sodium-gluconate and citrate were purchased from Sigma (Sigma, St. Louis, MO, USA), dissolved in PBS, and stored in aliquots at −80 °C.

    Article Title: Small CD63 + CD81+ extracellular vesicles induce autophagy via the FOXO3a pathway in pancreatic cancer cells during starvation.
    Article Snippet: .. PANC-1 and MiaPaca-2 cell lines (purchased to ATCC, Cat. CRL-1469 and CRL-1420) were cultured in DMEM high glucose medium (Thermo Fisher Scientific Cat. 12100046), while BxPC-3 cells (purchased to ATCC, Cat. CRL-1687) were cultured in RPMI medium (Gibco 23400-021). .. For the PDAC cell lines, the medium was supplemented with 10% fetal bovine serum (Internegocios S.A.) and 1% penicillin/streptomycin (Thermo Fisher Scientific Cat. 15140122).

    Article Title: Prolonged TGF-β locks NK cells in a dysfunctional state through persistent epigenetic remodeling of IRF, T-bet and EOMES binding sites
    Article Snippet: .. BxPC-3 cells (#CRL-1687; 61-year-old, female patient with pancreatic adenocarcinoma (PDAC)) were obtained from [ATCC] and cultured in RPMI + 10% FCS + 1% Pen/Strep. ..



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    Evaluation of the cytotoxicity of compounds AG1, AG2, AG3, and RK349 . a ) MTT assays for AGS and NUGC3 cells treated with increasing concentrations of AG2 (top) and AG3 (bottom) for 48 h ( n = 3). b ) Mean (-/+ SD) IC 50 and IC 75 values of the different compounds in the indicated cell line treated for 48 h ( n = 3). NR: not reached. c ) Graphic representation of the selectivity of each compound using the ratio of IC 50 for AGS (p53 WT) and NUGC3 (p53 Y220C) cells indicated in b and Fig S3b . d ) Representative wells of a clonogenic assay performed with NUGC3 cells treated or not (NT) for 48 h with oxaliplatin (IC 25 , 12 µM) and/or AG3 (IC 50 , 18 µM) ( n = 3). e ) Quantification of clonogenic assays performed as described in d , with the number of colonies obtained for non-treated cells set at 100%. ( n = 3). f ) MTT assays for <t>BxPC3</t> cells treated with increasing concentrations of AG3 for 48 h. g ) MTT assays for HCT116 and SW480 cells treated with increasing concentrations of RK349 and AG3 for 48 h.
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    Evaluation of the cytotoxicity of compounds AG1, AG2, AG3, and RK349 . a ) MTT assays for AGS and NUGC3 cells treated with increasing concentrations of AG2 (top) and AG3 (bottom) for 48 h ( n = 3). b ) Mean (-/+ SD) IC 50 and IC 75 values of the different compounds in the indicated cell line treated for 48 h ( n = 3). NR: not reached. c ) Graphic representation of the selectivity of each compound using the ratio of IC 50 for AGS (p53 WT) and NUGC3 (p53 Y220C) cells indicated in b and Fig S3b . d ) Representative wells of a clonogenic assay performed with NUGC3 cells treated or not (NT) for 48 h with oxaliplatin (IC 25 , 12 µM) and/or AG3 (IC 50 , 18 µM) ( n = 3). e ) Quantification of clonogenic assays performed as described in d , with the number of colonies obtained for non-treated cells set at 100%. ( n = 3). f ) MTT assays for <t>BxPC3</t> cells treated with increasing concentrations of AG3 for 48 h. g ) MTT assays for HCT116 and SW480 cells treated with increasing concentrations of RK349 and AG3 for 48 h.
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    Evaluation of the cytotoxicity of compounds AG1, AG2, AG3, and RK349 . a ) MTT assays for AGS and NUGC3 cells treated with increasing concentrations of AG2 (top) and AG3 (bottom) for 48 h ( n = 3). b ) Mean (-/+ SD) IC 50 and IC 75 values of the different compounds in the indicated cell line treated for 48 h ( n = 3). NR: not reached. c ) Graphic representation of the selectivity of each compound using the ratio of IC 50 for AGS (p53 WT) and NUGC3 (p53 Y220C) cells indicated in b and Fig S3b . d ) Representative wells of a clonogenic assay performed with NUGC3 cells treated or not (NT) for 48 h with oxaliplatin (IC 25 , 12 µM) and/or AG3 (IC 50 , 18 µM) ( n = 3). e ) Quantification of clonogenic assays performed as described in d , with the number of colonies obtained for non-treated cells set at 100%. ( n = 3). f ) MTT assays for BxPC3 cells treated with increasing concentrations of AG3 for 48 h. g ) MTT assays for HCT116 and SW480 cells treated with increasing concentrations of RK349 and AG3 for 48 h.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: In vitro screening of compounds for targeting gastric cancer with Y220C p53 mutation: a molecule combining zinc chelation and a Michael acceptor drives CDKN1 and BBC3 expression to restore a p53-dependent cytotoxicity

    doi: 10.1080/14756366.2026.2638836

    Figure Lengend Snippet: Evaluation of the cytotoxicity of compounds AG1, AG2, AG3, and RK349 . a ) MTT assays for AGS and NUGC3 cells treated with increasing concentrations of AG2 (top) and AG3 (bottom) for 48 h ( n = 3). b ) Mean (-/+ SD) IC 50 and IC 75 values of the different compounds in the indicated cell line treated for 48 h ( n = 3). NR: not reached. c ) Graphic representation of the selectivity of each compound using the ratio of IC 50 for AGS (p53 WT) and NUGC3 (p53 Y220C) cells indicated in b and Fig S3b . d ) Representative wells of a clonogenic assay performed with NUGC3 cells treated or not (NT) for 48 h with oxaliplatin (IC 25 , 12 µM) and/or AG3 (IC 50 , 18 µM) ( n = 3). e ) Quantification of clonogenic assays performed as described in d , with the number of colonies obtained for non-treated cells set at 100%. ( n = 3). f ) MTT assays for BxPC3 cells treated with increasing concentrations of AG3 for 48 h. g ) MTT assays for HCT116 and SW480 cells treated with increasing concentrations of RK349 and AG3 for 48 h.

    Article Snippet: BxPC3 cells (ATCC©, CRL1687) are from pancreatic cancer expressing Y220C mutant p53.

    Techniques: Clonogenic Assay