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zinc-buffered formalin z-fix  (Thermo Fisher)


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    Thermo Fisher zinc-buffered formalin z-fix
    Zinc Buffered Formalin Z Fix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+z/buffered+zinc+formalin/pmc12150058-191-9-12
    Average 90 stars, based on 1 article reviews
    zinc-buffered formalin z-fix - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Protective role of Parkin in skeletal muscle contractile and mitochondrial function
    Article Snippet: .. Net H 2 O 2 release by respiring mitochondria was measured in permeabilized fibres incubated in buffer Z (in m m : 110 K‐MES, 35 KCl, 1 EGTA, 5 K 2 HPO 4 , 3 MgCl 2 6H 2 O and 0.5 mg mL –1 BSA, 1.2 U mL –1 horseradish peroxidase, pH 7.3, 37°C) and the fluorescent probe Amplex Red (Thermo Fisher, Waltham, MA, USA) (20 μ m : excitation–emission: 563–587 nm) as described previously (Picard et al . 2008 ). ..

    Article Title: An extrinsic motor directs chromatin loop formation by cohesin.
    Article Snippet: The ring-shaped cohesin complex topologically entraps two DNA molecules to establish sister chromatid cohesion.. Cohesin also shapes the interphase chromatin landscape with wide-ranging implications for gene regulation, and cohesin is thought to achieve this by actively extruding DNA loops without topologically entrapping DNA.. The ‘loop extrusion’ hypothesis finds motivation from in vitro observations—whether this process underlies in vivo chromatin loop formation remains untested.

    Article Title: An extrinsic motor directs chromatin loop formation by cohesin
    Article Snippet: .. Cells were then resuspended in Buffer Z (50 mM Tris-HCl pH 7.5, 1 M sorbitol, 10 mM β-mercaptoethanol) and spheroplasted by addition of 250 μg/ml Zymolyase (100 T) in a 30 °C incubator at 200 rpm for 40–60 min. Spheroplasts were washed once by 4 °C PBS and then pelleted at 4000 rpm at 4 °C for 10 min. Chromatin was further cross-linked by suspending pellets in PBS supplemented with 3 mM disuccinimidyl glutarate (ThermoFisher) and incubated at 30 °C for 40 min with gentle shaking before quenching by addition of 400 mM glycine for 5 min at 30 °C. ..

    Article Title: Elucidating regulation of polyhydroxyalkanoate metabolism in R. eutropha: Identification of transcriptional regulators from phasin and depolymerase genes.
    Article Snippet: .. Cell pellets were resuspended in 240 μL Buffer Z (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4 and 50 mM β-mercaptoethanol, pH 7) and 5 μL lysozyme-DNase (8 mg mL-1 lysozyme and 1.25 mg mL-1 DNase) (Thermo Fisher Scientific, US), incubated for 10 min at 37 °C and centrifuged at 13000 ×g for 2 min. 200 μL supernatant were mixed with 600 μL Buffer Z and reactions were started by addition of 200 μL ONPG (Thermo Fisher Scientific, US) (4 mg mL-1, 19.5 mM NaH2PO4, 30.5 mM Na2HPO4, pH 7) and incubation at 28 °C. ..

    Article Title: Protective role of Parkin in skeletal muscle contractile and mitochondrial function
    Article Snippet: .. Mitochondrial H 2 O 2 emission Net H 2 O 2 release by respiring mitochondria was measured in permeabilized fibres incubated in buffer Z (in m m : 110 K‐MES, 35 KCl, 1 EGTA, 5 K 2 HPO 4 , 3 MgCl 2 6H 2 O and 0.5 mg mL –1 BSA, 1.2 U mL –1 horseradish peroxidase, pH 7.3, 37°C) and the fluorescent probe Amplex Red (Thermo Fisher, Waltham, MA, USA) (20 μ m : excitation–emission: 563–587 nm) as described previously (Picard et al . 2008 ). ..

    Sonication:

    Article Title: Concordance and interaction of guanine nucleotide dissociation inhibitor (RhoGDI) with RhoA in oogenesis and early development of the sea urchin
    Article Snippet: .. Pellets were resuspended in buffer Z (8 M urea, 100 mM NaCl, and 20 mM HEPES, pH 8.0) containing 20 mM imidazole, and sonicated prior to purification on a Ni-agarose column (Life Technologies, CA, USA), according to the manufacturer’s directions. ..

    Purification:

    Article Title: Concordance and interaction of guanine nucleotide dissociation inhibitor (RhoGDI) with RhoA in oogenesis and early development of the sea urchin
    Article Snippet: .. Pellets were resuspended in buffer Z (8 M urea, 100 mM NaCl, and 20 mM HEPES, pH 8.0) containing 20 mM imidazole, and sonicated prior to purification on a Ni-agarose column (Life Technologies, CA, USA), according to the manufacturer’s directions. ..

    Gentle:

    Article Title: An extrinsic motor directs chromatin loop formation by cohesin.
    Article Snippet: The ring-shaped cohesin complex topologically entraps two DNA molecules to establish sister chromatid cohesion.. Cohesin also shapes the interphase chromatin landscape with wide-ranging implications for gene regulation, and cohesin is thought to achieve this by actively extruding DNA loops without topologically entrapping DNA.. The ‘loop extrusion’ hypothesis finds motivation from in vitro observations—whether this process underlies in vivo chromatin loop formation remains untested.

    Article Title: An extrinsic motor directs chromatin loop formation by cohesin
    Article Snippet: .. Cells were then resuspended in Buffer Z (50 mM Tris-HCl pH 7.5, 1 M sorbitol, 10 mM β-mercaptoethanol) and spheroplasted by addition of 250 μg/ml Zymolyase (100 T) in a 30 °C incubator at 200 rpm for 40–60 min. Spheroplasts were washed once by 4 °C PBS and then pelleted at 4000 rpm at 4 °C for 10 min. Chromatin was further cross-linked by suspending pellets in PBS supplemented with 3 mM disuccinimidyl glutarate (ThermoFisher) and incubated at 30 °C for 40 min with gentle shaking before quenching by addition of 400 mM glycine for 5 min at 30 °C. ..

    Centrifugation:

    Article Title: Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment.
    Article Snippet: .. Nuclei and debris were removed by centrifugation (15,000 g, 20 minutes), and the supernatant was dialyzed against one change of buffer Z (20 mM Tris-HCl (pH 7.8), 0.1 M KCl, 0.2 mM EDTA, and 20% glycerol) for at least 3 hours at 4°C in dialysis cassettes (Dialyze Z; Pierce, Inc.). ..



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    Image Search Results


    MC38-bearing young or aged mice were treated with anti–PD-L1 Ab. ( A – C ) Lung-infiltrating CD45 + cells were isolated from MC38-bearing young or aged mice that were treated with anti–PD-L1 Ab, and then were analyzed by scRNA-seq. Cluster analysis was performed and plotted by UMAP dimensionality reduction. Subclustering was performed on the data from the Cd4 + T cell populations in young and aged mice, and lower bar plots in A indicate their frequencies. Dot plots shown in B represent the expression of canonical marker genes across the indicated Cd4 + subclusters. Relative expression of indicated genes across subclustered populations from young and aged mice are shown in C . ( D ) Representative images of DAPI staining (left) and in situ RNA hybridization (right; white, ICOS; red, CD19; green, CD4) in lung from aged mice are shown. Scale bars: 100 μm (left) and 50 μm (right). ( E ) Representative dot plots of CD45 iv- cell populations within the lungs are shown. ( F ) Frequencies of indicated lung-infiltrating CD45 – cell populations were assessed in mice treated with indicated Abs. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA followed by Tukey-Kramer post hoc test.

    Journal: JCI Insight

    Article Title: ICOS + CD4 + T cells define a high susceptibility to anti–PD-1 therapy–induced lung pathogenesis

    doi: 10.1172/jci.insight.186483

    Figure Lengend Snippet: MC38-bearing young or aged mice were treated with anti–PD-L1 Ab. ( A – C ) Lung-infiltrating CD45 + cells were isolated from MC38-bearing young or aged mice that were treated with anti–PD-L1 Ab, and then were analyzed by scRNA-seq. Cluster analysis was performed and plotted by UMAP dimensionality reduction. Subclustering was performed on the data from the Cd4 + T cell populations in young and aged mice, and lower bar plots in A indicate their frequencies. Dot plots shown in B represent the expression of canonical marker genes across the indicated Cd4 + subclusters. Relative expression of indicated genes across subclustered populations from young and aged mice are shown in C . ( D ) Representative images of DAPI staining (left) and in situ RNA hybridization (right; white, ICOS; red, CD19; green, CD4) in lung from aged mice are shown. Scale bars: 100 μm (left) and 50 μm (right). ( E ) Representative dot plots of CD45 iv- cell populations within the lungs are shown. ( F ) Frequencies of indicated lung-infiltrating CD45 – cell populations were assessed in mice treated with indicated Abs. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA followed by Tukey-Kramer post hoc test.

    Article Snippet: The target mRNA in the tissues was hybridized by incubating in RNAscope buffered Z probes for Icos (Advanced Cell Diagnostics, 552451), Cd4 (Advanced Cell Diagnostics, 406841-C2), and Cd19 (Advanced Cell Diagnostics, 314711-C3) at 40°C for 2 hours.

    Techniques: Isolation, Expressing, Marker, Staining, In Situ, Hybridization

    Tumor-bearing young or aged mice were administered with anti–PD-L1 Ab. Anti-ICOSL Ab was injected to block the ICOS-ICOSL interaction. ( A and B ) Representative dot plots of CD45 iv- lung-infiltrating cell populations from MC38-bearing mice ( A ) and their frequencies ( B ) are shown. ( C and D ) Representative images of CD138 IHC in the lung of tumor-bearing aged mice ( C ), and the percentage area of TLS (left) and CD138 + plasma cells (right) in each lung section ( D ) are shown. Scale bars: 100 μm. ( E ) The levels of SP-D in the serum are shown. ( F ) Lung dysfunction in tumor-bearing mice was assessed by mechanical ventilation system. Data are representative of 3 independent experiments with similar results and presented as mean ± SEM ( n = 4–8). * P < 0.05; ** P < 0.01; *** P < 0.001 by 1-way ANOVA followed by Tukey-Kramer post hoc test.

    Journal: JCI Insight

    Article Title: ICOS + CD4 + T cells define a high susceptibility to anti–PD-1 therapy–induced lung pathogenesis

    doi: 10.1172/jci.insight.186483

    Figure Lengend Snippet: Tumor-bearing young or aged mice were administered with anti–PD-L1 Ab. Anti-ICOSL Ab was injected to block the ICOS-ICOSL interaction. ( A and B ) Representative dot plots of CD45 iv- lung-infiltrating cell populations from MC38-bearing mice ( A ) and their frequencies ( B ) are shown. ( C and D ) Representative images of CD138 IHC in the lung of tumor-bearing aged mice ( C ), and the percentage area of TLS (left) and CD138 + plasma cells (right) in each lung section ( D ) are shown. Scale bars: 100 μm. ( E ) The levels of SP-D in the serum are shown. ( F ) Lung dysfunction in tumor-bearing mice was assessed by mechanical ventilation system. Data are representative of 3 independent experiments with similar results and presented as mean ± SEM ( n = 4–8). * P < 0.05; ** P < 0.01; *** P < 0.001 by 1-way ANOVA followed by Tukey-Kramer post hoc test.

    Article Snippet: The target mRNA in the tissues was hybridized by incubating in RNAscope buffered Z probes for Icos (Advanced Cell Diagnostics, 552451), Cd4 (Advanced Cell Diagnostics, 406841-C2), and Cd19 (Advanced Cell Diagnostics, 314711-C3) at 40°C for 2 hours.

    Techniques: Injection, Blocking Assay, Clinical Proteomics

    ( A ) CD4 + T cells were isolated from the lungs of aged mice treated anti–PD-1 therapy and then were stimulated with anti-CD3/anti-CD28 Abs together with anti-ICOS or anti-CD153 agonistic Ab ex vivo. Fold inductions of indicated mRNA expression relative to that in control Ab-stimulated cells are shown. ( B ) Concentration of IL-21 or CXCL13 in BALF isolated from young or aged mice with indicated treatment was measured. Data are mean ± SEM ( n = 3–6). One-way ANOVA followed by Tukey’s post hoc test was used. ( C – F ) WT or IL-21–deficient aged mice were treated with anti–PD-1 and anti-ICOSL Abs, and inoculated intranasally (i.n.) with recombinant IL-21. Representative plots of CD95 + GL7 + cells ( C ) and IgG + cells ( D ) among lung-infiltrating B cells, their frequencies ( E ), and serum concentrations of SP-D ( F ) are shown. Data are mean ± SEM, and the results from 2 independent experiments were combined (n = 4–6 per experiment). Then, 1-way ANOVA followed by Tukey-Kramer post hoc test was conducted for aged WT or IL-21–KO groups separately. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: JCI Insight

    Article Title: ICOS + CD4 + T cells define a high susceptibility to anti–PD-1 therapy–induced lung pathogenesis

    doi: 10.1172/jci.insight.186483

    Figure Lengend Snippet: ( A ) CD4 + T cells were isolated from the lungs of aged mice treated anti–PD-1 therapy and then were stimulated with anti-CD3/anti-CD28 Abs together with anti-ICOS or anti-CD153 agonistic Ab ex vivo. Fold inductions of indicated mRNA expression relative to that in control Ab-stimulated cells are shown. ( B ) Concentration of IL-21 or CXCL13 in BALF isolated from young or aged mice with indicated treatment was measured. Data are mean ± SEM ( n = 3–6). One-way ANOVA followed by Tukey’s post hoc test was used. ( C – F ) WT or IL-21–deficient aged mice were treated with anti–PD-1 and anti-ICOSL Abs, and inoculated intranasally (i.n.) with recombinant IL-21. Representative plots of CD95 + GL7 + cells ( C ) and IgG + cells ( D ) among lung-infiltrating B cells, their frequencies ( E ), and serum concentrations of SP-D ( F ) are shown. Data are mean ± SEM, and the results from 2 independent experiments were combined (n = 4–6 per experiment). Then, 1-way ANOVA followed by Tukey-Kramer post hoc test was conducted for aged WT or IL-21–KO groups separately. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: The target mRNA in the tissues was hybridized by incubating in RNAscope buffered Z probes for Icos (Advanced Cell Diagnostics, 552451), Cd4 (Advanced Cell Diagnostics, 406841-C2), and Cd19 (Advanced Cell Diagnostics, 314711-C3) at 40°C for 2 hours.

    Techniques: Isolation, Ex Vivo, Expressing, Control, Concentration Assay, Recombinant

    MC38-bearing young or aged mice were treated with control or anti–PD-(L)1 Ab. ( A and B ) The representative dot plots of blood-circulating CD4 + T cells 13 days after tumor inoculation ( A ), and kinetic changes in frequencies of the indicated populations ( B ) are shown ( n = 5–6). ( C ) Frequencies of Foxp3 – or Foxp3 + ICOS + CD4 + T cells in the peripheral blood were analyzed ( n = 8–9). ** P < 0.01, *** P < 0.001 by 1-way ANOVA followed by Tukey’s post hoc test. ( D ) Correlation between the frequencies of indicated populations and the concentration of SP-D is shown. Simple linear regression analysis was conducted. Data are mean ± SEM from more than 2 independent experiments with similar results.

    Journal: JCI Insight

    Article Title: ICOS + CD4 + T cells define a high susceptibility to anti–PD-1 therapy–induced lung pathogenesis

    doi: 10.1172/jci.insight.186483

    Figure Lengend Snippet: MC38-bearing young or aged mice were treated with control or anti–PD-(L)1 Ab. ( A and B ) The representative dot plots of blood-circulating CD4 + T cells 13 days after tumor inoculation ( A ), and kinetic changes in frequencies of the indicated populations ( B ) are shown ( n = 5–6). ( C ) Frequencies of Foxp3 – or Foxp3 + ICOS + CD4 + T cells in the peripheral blood were analyzed ( n = 8–9). ** P < 0.01, *** P < 0.001 by 1-way ANOVA followed by Tukey’s post hoc test. ( D ) Correlation between the frequencies of indicated populations and the concentration of SP-D is shown. Simple linear regression analysis was conducted. Data are mean ± SEM from more than 2 independent experiments with similar results.

    Article Snippet: The target mRNA in the tissues was hybridized by incubating in RNAscope buffered Z probes for Icos (Advanced Cell Diagnostics, 552451), Cd4 (Advanced Cell Diagnostics, 406841-C2), and Cd19 (Advanced Cell Diagnostics, 314711-C3) at 40°C for 2 hours.

    Techniques: Control, Concentration Assay

    ( A ) The frequency of ICOS + cells in peripheral CD4 + T cells was analyzed before (Pre) and 4 weeks after initial anti–PD-(L)1 therapy (On) in patients with ( n = 8) or without ( n = 13) the irAE pneumonitis. * P < 0.05 by Wilcoxon’s signed-rank test. ( B ) Changes in the frequencies of indicated CD4 + or CD8 + T cell subsets during 4 weeks of anti–PD-(L)1 therapy in patients with ( n = 34) or without ( n = 13) irAEs, were analyzed. Red dots indicate the values for patients with pneumonitis. ( C ) Changes in the frequency of ICOS + CD4 + T cells in patients with indicated irAEs are shown. “Others” includes symptoms with infusion reaction, fever, adrenal irAEs, carditis, diarrhea, hypophysitis, and neurologic irAEs. * P < 0.05; ** P < 0.01 by Mann-Whitney U test. ( D ) Univariate (ΔICOS, fold change in CXCL13 or IL-6) and multivariate (ΔICOS plus changes in CXCL13) ROC analyses of the predictive values for pneumonitis development. ( E ) Distribution of indicated values in the combined predictive model for pneumonitis incidence in patients with pneumonitis ( n = 8) or without ( n = 12) irAEs. Adonis test based on the Bray-Curtis distance was used. ( F ) Kaplan-Meier plots of progression-free survival of patients with NSCLC stratified according to the median value of the change in peripheral ICOS + CD4 + T cells are shown. Log-rank (Mantel-Cox) test and univariate Cox proportional hazards analyses were performed.

    Journal: JCI Insight

    Article Title: ICOS + CD4 + T cells define a high susceptibility to anti–PD-1 therapy–induced lung pathogenesis

    doi: 10.1172/jci.insight.186483

    Figure Lengend Snippet: ( A ) The frequency of ICOS + cells in peripheral CD4 + T cells was analyzed before (Pre) and 4 weeks after initial anti–PD-(L)1 therapy (On) in patients with ( n = 8) or without ( n = 13) the irAE pneumonitis. * P < 0.05 by Wilcoxon’s signed-rank test. ( B ) Changes in the frequencies of indicated CD4 + or CD8 + T cell subsets during 4 weeks of anti–PD-(L)1 therapy in patients with ( n = 34) or without ( n = 13) irAEs, were analyzed. Red dots indicate the values for patients with pneumonitis. ( C ) Changes in the frequency of ICOS + CD4 + T cells in patients with indicated irAEs are shown. “Others” includes symptoms with infusion reaction, fever, adrenal irAEs, carditis, diarrhea, hypophysitis, and neurologic irAEs. * P < 0.05; ** P < 0.01 by Mann-Whitney U test. ( D ) Univariate (ΔICOS, fold change in CXCL13 or IL-6) and multivariate (ΔICOS plus changes in CXCL13) ROC analyses of the predictive values for pneumonitis development. ( E ) Distribution of indicated values in the combined predictive model for pneumonitis incidence in patients with pneumonitis ( n = 8) or without ( n = 12) irAEs. Adonis test based on the Bray-Curtis distance was used. ( F ) Kaplan-Meier plots of progression-free survival of patients with NSCLC stratified according to the median value of the change in peripheral ICOS + CD4 + T cells are shown. Log-rank (Mantel-Cox) test and univariate Cox proportional hazards analyses were performed.

    Article Snippet: The target mRNA in the tissues was hybridized by incubating in RNAscope buffered Z probes for Icos (Advanced Cell Diagnostics, 552451), Cd4 (Advanced Cell Diagnostics, 406841-C2), and Cd19 (Advanced Cell Diagnostics, 314711-C3) at 40°C for 2 hours.

    Techniques: MANN-WHITNEY