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bt-474  (ATCC)


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    Structured Review

    ATCC bt-474
    Bt 474, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bt+474/BT-474/custom%40htb-20%4010%2E1016%2Fj%2Ebbrc%2E2026%2E154365
    Average 99 stars, based on 3998 article reviews
    bt-474 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    Article Title: Single-cell and spatial profiling of cysteine cathepsins identifies tumor states relevant to antibody-drug conjugates in breast cancer
    Article Snippet: .. BT-474 (ATCC HTB-20, RRID: CVCL_0179), MCF-7 (ATCC HTB-22, RRID: CVCL_0031), and MDA-MB-231 (ATCC HTB-26, RRID: CVCL_0062) breast cancer cell lines were cultured at 37°C in a humidified incubator with 5% CO 2 . .. Cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Cat. No. 41965-039) supplemented with 10% fetal bovine serum (FBS; Gibco, Cat. No. A5256801), L-glutamine, sodium pyruvate, penicillin (100 U/mL), and streptomycin (0.1 mg/mL).

    Article Title: Recombinant herpes simplex virus for multiple targeting and use thereof
    Article Snippet: .. For breast cancer cell lines MDA-MB-231 (ATCC, HTB-26), MCF-7 (ATCC, HTB-22), and BT-474 (ATCC, HTB-20), and an ovarian cancer cell line SK-OV-3 (ATCC, HTB-77), culture was performed using DMEM containing 100 U/ml penicillin/100 μg/ml streptomycin (Welgene) and 10% FBS, and for a breast cancer cell line MDA-MB-453 (ATCC, HTB-131), culture was performed using an RPMI 1640 medium containing 100 U/ml penicillin/100 μg/ml streptomycin (Welgene) and 10% FBS. ..

    Article Title: The cellular stress sensor HSPB1 regulates the membrane localization of amino acid transporter SLC7A5 in breast cancer.
    Article Snippet: .. MCF-7, T47-D, ZR-75-1, BT-474, AU565, HCC1569, MDA-MB 231, Hs578T, BT-549, and HEK293T cell lines were obtained from ATCC. .. MCF-7 cells were cultured in EMEM (Nacalai) supplemented with 10% FBS, 10 g/ml insulin, 1 mM sodium pyruvate, and penicillin- streptomycin (Nacalai).

    Article Title: Targeting SIK2 with GRN-300 Potentiates Paclitaxel Efficacy in Triple-Negative Breast Cancer.
    Article Snippet: .. Human breast cancer cell lines (MDA-MB-231 (MB-231), MDA-MB-436 (MB-436), MDA-MB-468 (MB-468), MDA-MB-453 (MB-453), SUM159, HCC1143, HCC1395, HCC1428, HCC1569, HCC1937, HCC1954, HCC202, HCC38, HCC70, BT-474, T47D, MCF7, SKBr3, and ZR75-1) were obtained from ATCC (Manassas, VA, USA) and Cal51 was purchased from Creative Bioarray (Shirley, NY, USA). ..

    Article Title: Development and Validation of a Cuproptosis-Based Risk Score Model for Predicting Neoadjuvant Chemotherapy Response in Breast Cancer: A Transcriptomic Analysis.
    Article Snippet: .. MCF-7 (ATCC HTB-22), BT-474 (HTB-20), MDA-MB-231 (HTB26), and MCF10A (ATCC CRL-10317) cell lines were used for RNA extraction with TRIzol reagent (Invitrogen, 15596018). ..

    Cell Culture:

    Article Title: Single-cell and spatial profiling of cysteine cathepsins identifies tumor states relevant to antibody-drug conjugates in breast cancer
    Article Snippet: .. BT-474 (ATCC HTB-20, RRID: CVCL_0179), MCF-7 (ATCC HTB-22, RRID: CVCL_0031), and MDA-MB-231 (ATCC HTB-26, RRID: CVCL_0062) breast cancer cell lines were cultured at 37°C in a humidified incubator with 5% CO 2 . .. Cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Cat. No. 41965-039) supplemented with 10% fetal bovine serum (FBS; Gibco, Cat. No. A5256801), L-glutamine, sodium pyruvate, penicillin (100 U/mL), and streptomycin (0.1 mg/mL).

    Article Title: The adipogenesis-related gene CEBPD as a prognostic biomarker and functional tumor suppressor in breast cancer.
    Article Snippet: .. Cell culture The normal breast epithelial cell line HBL-100 and the breast cancer cell lines MCF-7, BT-474, and HCC1806 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. All cell lines were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco).

    RNA Extraction:

    Article Title: Development and Validation of a Cuproptosis-Based Risk Score Model for Predicting Neoadjuvant Chemotherapy Response in Breast Cancer: A Transcriptomic Analysis.
    Article Snippet: .. MCF-7 (ATCC HTB-22), BT-474 (HTB-20), MDA-MB-231 (HTB26), and MCF10A (ATCC CRL-10317) cell lines were used for RNA extraction with TRIzol reagent (Invitrogen, 15596018). ..



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