fluorophore conjugated mouse monoclonal antibodies against cd31  (Bioss)

Journal: Kidney Research and Clinical Practice

Article Title: Active maintenance of endothelial cells prevents kidney fibrosis

doi: 10.23876/j.krcp.2017.36.4.329

Figure Lengend Snippet: (A, B) Significantly reduced interstitial fibrosis following treatment with 12-(3-adamantan-1-ylureido)-dodecanoic acid (AUDA) in a unilateral ureteral obstruction (UUO) model. (C–E) Immunofluorescence analysis. AUDA significantly reduced the level of fibroblast-specific protein 1 (FSP-1) positive fibrosis, the proportion of FSP-1/CD31-positive cells, and preserved the peritubular capillary network. Arrows indicate FSP-1/CD3 double-positive cells. (F–H) AUDA also reduced the level of αSMA-positive fibrosis and the proportion of αSMA/CD31 double-positive cells. (I, J) Inhibition of sEH down-regulated expression of FSP-1 as demonstrated by real-time PCR (I) and Western blotting (J). Values are given as mean ± standard error of mean (n = 6 per group for each experiment). * P < 0.05, ** P < 0.01, *** P < 0.001; by ANOVA test.

Article Snippet: Isolated kidney mononuclear cells were incubated with fluorophore-conjugated mouse monoclonal antibodies against CD31 and FSP-1 (Bioss, Woburn, MA, USA).

Techniques: Immunofluorescence, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Kidney Research and Clinical Practice

Article Title: Active maintenance of endothelial cells prevents kidney fibrosis

doi: 10.23876/j.krcp.2017.36.4.329

Figure Lengend Snippet: 12-(3-adamantan-1-ylureido)-dodecanoic acid (AUDA) treatment suppressed transforming growth factor (TGF)-β1 and TGF-β2 (A) and increased expression of angiogenic molecules such as von Willebrand factor (vWF), CD31, and VE-cadherin (B). Values are given as mean ± standard error of mean (n = 6 per group for each experiment). * P < 0.05, ** P < 0.01; by ANOVA test.

Article Snippet: Isolated kidney mononuclear cells were incubated with fluorophore-conjugated mouse monoclonal antibodies against CD31 and FSP-1 (Bioss, Woburn, MA, USA).

Techniques: Expressing

Journal: Kidney Research and Clinical Practice

Article Title: Active maintenance of endothelial cells prevents kidney fibrosis

doi: 10.23876/j.krcp.2017.36.4.329

Figure Lengend Snippet: (A) Flow-cytometric analysis. The proportion of fibroblast-specific protein 1 (FSP-1)/CD31 double-positive cells among all FSP-1–positive cells was reduced by 12-(3-adamantan-1-ylureido)-dodecanoic acid (AUDA) treatment. (B) Immunofluorescence analyses of CD31 cells from isolated intrarenal mononuclear cells obtained from cytospin preparations. AUDA treatment reduced the proportion of von Willebrand factor/FSP-1 double-positive cells.

Article Snippet: Isolated kidney mononuclear cells were incubated with fluorophore-conjugated mouse monoclonal antibodies against CD31 and FSP-1 (Bioss, Woburn, MA, USA).

Techniques: Immunofluorescence, Isolation

Journal: Kidney Research and Clinical Practice

Article Title: Active maintenance of endothelial cells prevents kidney fibrosis

doi: 10.23876/j.krcp.2017.36.4.329

Figure Lengend Snippet: (A, B) Recombinant transforming growth factor (rTGF)-β2–induced fibroblast-like changes and fibroblast-specific protein 1 (FSP-1) expression in HUVECs were reversed by 12-(3-adamantan-1-ylureido)-dodecanoic acid (AUDA) treatment. (C–E) AUDA treatment increased expression of CD31 and reduced expression of αSMA and FSP-1. All values are given as mean ± standard error of mean (n = 4 per group for each experiment). * P < 0.05, ** P < 0.01, *** P < 0.001; by ANOVA test.

Article Snippet: Isolated kidney mononuclear cells were incubated with fluorophore-conjugated mouse monoclonal antibodies against CD31 and FSP-1 (Bioss, Woburn, MA, USA).

Techniques: Recombinant, Expressing