bsm 33042m  (Bioss)

Journal: Renal Failure

Article Title: Esaxerenone attenuates renal injury by inhibiting NR3C2-mediated inflammatory and fibrotic pathways

doi: 10.1080/0886022X.2026.2709945

Figure Lengend Snippet: Information of primary antibodies used in western blot.

Article Snippet: H3 , 1:2000 , Bioss , Bsm-33042M.

Techniques: Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice

doi: 10.1155/2019/5189819

Figure Lengend Snippet: Kidney-specific VEGFR2 knockdown alleviates NRLP3-dependent inflammation in the kidney of the HFD-treated mice. (a) Representative immunoblots for NLRP3, procaspase 1, cleaved caspase 1, pro-IL1 β , cleaved IL1 β , and β -actin. (b) Relative density analysis of the NLRP3, cleaved caspase 1, and cleaved IL1 β protein bands. Relative densities are expressed as the ratio of NRLP3 to β -actin, cleaved caspase 1 to procaspase 1 (expressed as cleaved/procaspase 1), and cleaved IL1 β to pro-IL1 β (expressed as cleaved/pro-IL1 β ). (c) Representative immunoblots for cytoplasmic NF- κ B p65 (C-NF- κ B p65), nuclear NF- κ B p65 (N-NF- κ B p65), β -actin, and histone H3 (H3). (d) Relative density analysis of the C-NF- κ B p65 and N-NF- κ B p65 protein bands. Relative densities are expressed as the ratio of C-NF- κ B p65 to β -actin and N-NF- κ B p65 to H3. (e) Representative immunoblots for TNF α , IL6, iNOS, and β -actin. (f) Relative density analysis of the TNF α , IL6, and iNOS protein bands. Relative densities are expressed as the ratio of TNF α , IL6, and iNOS to β -actin. All values are expressed as mean ± SEM ((a–f), n = 5). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 vs. the CD scramble group, and ## p < 0.01 and ### p < 0.001 vs. the HFD scramble group.

Article Snippet: The optic density (OD) values of detected bands were measured with the Scion Image analysis software (Scion, Frederick, MD, USA) and were normalized to mouse anti- β -actin (66009-1, 1 : 5000, Proteintech, Wuhan, China) or mouse anti-histone H3 (Bsm-33042M, 1 : 5000, Bioss, Beijing, China) as internal controls (OD detected protein/OD internal control).

Techniques: Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice

doi: 10.1155/2019/5189819

Figure Lengend Snippet: α -Lipoic acid decreases ROS-triggered inflammation in the kidney of HFD-treated mice. (a) ROS levels were assessed by dihydroethidium (DHE) staining. (b) DHE fluorescence intensity was measured by the IPP, and results are expressed by the ratio of the integrated optical density of the DHE-positive area to the DAPI. (c) Representative immunoblots for NLRP3, procaspase 1, cleaved caspase 1, pro-IL1 β , cleaved IL1 β , and β -actin. (d) Relative density analysis of NLRP3, cleaved caspase 1, and cleaved IL1 β protein bands. Relative densities are expressed as the ratio of NRLP3 to β -actin, cleaved caspase 1 to procaspase 1 (expressed as cleaved/procaspase 1), and cleaved IL1 β to Pro-IL 1 β (expressed as cleaved/pro-IL1 β ). (e) Representative immunoblots for cytoplasmic NF- κ B p65 (C-NF- κ B p65), nuclear NF- κ B p65 (N-NF- κ B p65), β -actin, and histone H3 (H3). (f) Relative density analysis of the C-NF- κ B p65 and N-NF- κ B p65 protein bands. Relative densities are expressed as the ratio of C-NF- κ B p65 to β -actin and N-NF- κ B p65 to H3. (g) Representative immunoblots for TNF α , IL6, iNOS, and β -actin. (h) Relative density analysis of TNF α , IL6, and iNOS protein bands. Relative densities are expressed as the ratio of TNF α , IL6, and iNOS to β -actin. All values are expressed as mean ± SEM ((a–h), n = 5). ∗∗∗ p < 0.001 vs. the CD vehicle group, and ### p < 0.001 vs. the HFD vehicle group.

Article Snippet: The optic density (OD) values of detected bands were measured with the Scion Image analysis software (Scion, Frederick, MD, USA) and were normalized to mouse anti- β -actin (66009-1, 1 : 5000, Proteintech, Wuhan, China) or mouse anti-histone H3 (Bsm-33042M, 1 : 5000, Bioss, Beijing, China) as internal controls (OD detected protein/OD internal control).

Techniques: Staining, Fluorescence, Western Blot