Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Highly Expressed Granulocyte Colony-Stimulating Factor (G-CSF) and Granulocyte Colony-Stimulating Factor Receptor (G-CSFR) in Human Gastric Cancer Leads to Poor Survival
doi: 10.12659/MSM.909128
Figure Lengend Snippet: G-CSF/G-CSFR correlation with GC patient survival. ( A ) The concentrations of G-CSF, G-CSFR and VEGF-A were measured in patients who had died and those who survived by ELISA. The G-CSF concentration was significantly higher in patients who had died than in those who had survived. ( B ) Kaplan-Meier postoperative survival analysis of OS for GC (n=70) using immunohistochemistry. High G-CSF expression was associated with poor OS. * P <0.05.
Article Snippet: Sections were incubated with anti-G-CSF (1: 15, BIOBASIC, China), anti-G-CSFR (1: 15, BIOBASIC, China), and anti-VEGF-A (1: 50, BIOSS, China) polyclonal rabbit antibodies or with PBS instead of a primary antibody as the negative control for 60 min at room temperature.
Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Immunohistochemistry, Expressing