bs 1287r (Bioss)

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Bioss bs 1287r
Bs 1287r, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bs+1287r/NFKBIA+IKB+alpha+Polyclonal+Antibody/pmc12729743-31-4-2
Average 94 stars, based on 78 article reviews

bs 1287r - by Bioz Stars, 2026-09

94/100 stars

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Western Blot:

Article Title: Biglycan promotes the chemotherapy resistance of colon cancer by activating NF-κB signal transduction.
Article Snippet: Biglycan (BGN) is overexpressed in cancer stem cells of colon cancer and induces the activation of NF-κB pathway which contributes to the chemotherapy resistance of diverse cancer types.. Therefore, we hypothesized that the overexpression of BGN also promoted the development of multiple drug resistance (MDR) in colon cancer via NF-κB pathway.. The expression of BGN was bilaterally modulated in colon cancer cell lines HT-29 and SW-480 and the effect of treatments on the cell proliferation and resistance to 5-FU was assessed.

Polyacrylamide Gel Electrophoresis:

Incubation:

Article Title: Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-κB.
Article Snippet: The section was blocked with goat serum (cat. no. ZLI-9056; ZSGB-BIO; OriGene Technologies, Inc.) for 30min at room temperature. .. The primary antibodies, IKKb (Bioss, bs-2910R, 1:100) and nuclear factor of kappa light chain polypeptide gene enhancer in B-cells inhibitor alpha ([IjBa]; Bioss, bs-1287R, 1:100), were incubated with the sections overnight at 4 C. At room temperature, secondary antibodies were added to sections and incubated for 20min in the dark after which they were rinsed three times in PBS for 3min per wash. ..

Article Title: Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-κB
Article Snippet: The section was blocked with goat serum (cat. no. ZLI-9056; ZSGB-BIO; OriGene Technologies, Inc.) for 30 min at room temperature. .. The primary antibodies, IKKβ (Bioss, bs-2910R, 1:100) and nuclear factor of kappa light chain polypeptide gene enhancer in B-cells inhibitor alpha ([IκBα]; Bioss, bs-1287R, 1:100), were incubated with the sections overnight at 4 °C. ..

Article Title: Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity.
Article Snippet: The protein concentration was determined using a BCA protein quantitative kit (Beyotime Institute of Biotechnology). .. Protein samples (20-40 μg/lane) were resolved by 8-15% SDS-PAGE, blotted onto polyvinylidene fluoride membranes, blocked with 5% skim milk and incubated with primary antibodies against ENDOCAN (1:300; cat. no. PAC463Hu01; Cloud-Clone Corp., Wuhan, China), proliferating cell nuclear antigen (PCNA; 1:500; cat. no. 10205-2-AP; Wuhan Sanying Biotechnology, Wuhan, China), cyclin D1 (1:500; cat. no. AP2612d; Abgent, Inc., San Diego, CA, USA), B-cell lymphoma 2 (BCL2; 1:500; cat. no. D160117; Sangon Biotech Co., Ltd., Shanghai, China), BCL-2-associated X protein (BAX; 1:1,000; D120073; Sangon Biotech Co., Ltd.), cleaved caspase 3 (1:1,000; cat. no. ab2302; Abcam, Cambridge, MA, USA), cleaved caspase 9 (1:1,000; cat. no. 9501; CST Biological Reagents Co., Ltd., Shanghai, China), cleaved poly (ADP-ribose) polymerase (PARP; 1:500; cat. no. ab32561; Abcam), NF-κB, P65 (1:1,000; cat. no. 10745-1-AP; Wuhan Sanying Biotechnology), phosphorylated (p-)P65 (1:400; cat. no. BM3994; Boster Biological Technology, Pleasanton, CA, USA), NF-κB inhibitor α (IκBα) (1:500; cat. no. bs-1287R; BIOSS, Beijing, China), p-IκBα (1:500; cat. no. bs-2513R; BIOSS), β-actin (1:500; bsm-33139M; BIOSS), and histone H3 (1:5,000; cat. no. GTX122148; GeneTex, Inc., Irvine, CA, USA) overnight at 4 ̊C. .. Subsequently, the membranes were treated with horseradish peroxidase (HRP)-labeled goat anti-rabbit antibodies (1:5,000; cat. no. A0208; Beyotime Institute of Biotechnology) at 37 ̊C for 45 min.

Article Title: Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats.
Article Snippet: .. The blots were incubated with anti-inducible nitric oxide synthase (inoS) antibody (1:400; Ba0362, Boster Biological Technology), anticyclooxygenase (COX)-2 antibody (1:400; BA0738, Boster Biological Technology), anti-high-mobility group box 1 (hMGB1) antibody (1:400; Ba4277, Boster Biological Technology), anti-p65 antibody (1:400; Ba0610, Boster Biological Technology), anti-inhibitors of NFκBα (IκBα) antibody (1:500; bs-1287R, Bioss Antibodies Inc., Woburn, Ma, uSa), anti-p-p65 antibody (1:500; bs-0982R, Bioss Antibodies Inc.), anti-Toll-like receptor (TLR) 4 antibody (1:200; sc-293072, Santa Cruz Biotechnology, Dallas, TX, USA), anti-myeloid differentiation primary response 88 (MyD88) antibody (1:200; sc-8196, Santa Cruz Biotechnology), and anti-β-actin antibody (1:1,000; sc-47778, Santa Cruz Biotechnology) at 4°C overnight. .. The membranes were then washed and incubated with secondary antibodies (1:5,000, Beyotime Institute of Biotechnology, haimen, China) for 45 min at room temperature.

Blocking Assay:

Article Title: Melatonin inhibits bovine viral diarrhea virus replication by ER stress-mediated NF-κB signal pathway and autophagy in MDBK cells
Article Snippet: Protein samples were added on 10-12% sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) for electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corp., Billerica, MA, USA). .. Blocking was performed with 5% skimmed milk for 2 h at room temperature followed by overnight at 4°C with the indicated primary antibody(BVDV E2, VMRD, 157; GRP78, Wanlei Bio, WL3357; PERK, Wanlei Bio, WL3378; eIF2α, Wanlei Bio, WL01909; phospho-eIF2α (p-eIF2α), Cell Signaling Technology, 3398; ATF4, Wanlei Bio, WL02330; CHOP, Abmart, T56694; p65, Cell Signaling Technology, 8242; phospho-p65 (p-p65), Cell Signaling Technology, 3033); IκB-α, Bioss, bs-1287R; phospho-IκB-α (p-IκB-α), Bioss, bs-18129R; LC3A/B, Cell Signaling Technology, 4108; p62, Cell Signaling Technology, 5114). ..

other:

Article Title: Taurine Alleviates Inflammation, Oxidative Stress, Apoptosis, and Uterus Microbiota Dysregulation of Endometritis by Inhibiting PI3K-AKT/MAPK/NF-κB Pathways in Mice
Article Snippet: IκBα , Bioss , bs-1287R , 1:1000.

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