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anti brd2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti brd2
    Anti Brd2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brd2/pm41942733-275-38-40
    Average 86 stars, based on 1 article reviews
    anti brd2 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: Inhibition of BRD proteins suppresses the phenotype of uterine fibroids
    Article Snippet: Specific protein bands were visualized using ChemiDoc XRS molecular imager (Bio-Rad, Hercules, CA, USA).

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy
    Article Snippet: PBMCs were cultured overnight (19 h) with a 10 μM top dose, 7-point, 2-fold dilution curve of TAPTAC1 or A1874 (Selleckchem, #S8785), or 0.1% DMSO vehicle (Sigma D2650-100ML) control in a total volume of 100 μL.

    Saline:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: For the proteasome inhibition experiment, SJSA-X cells were cultured, treated with TAPTAC1, and processed as above except that bortezomib (50 nM) was added and assessment time points were at 4, 8, and 24 h and 3–8% TrisAcetate SDS-PAGE gels (Invitrogen) were used for electrophoresis. .. The membranes were blocked in a solution of 5% milk prepared in PBS-T (Phosphate Buffered Saline, 0.01% Tween) and incubated with primary antibodies overnight at 4 ◦ C, 1:1000 in 3% BSA (p53 [Millipore, #OP43-100UG, RRID: AB_10682938], p21 [Cell Signaling Technology, #2947, RRID: AB_823586], HDM2 [Millipore, #OP46-100UG, RRID: AB_10681293], HDMX [Fortis Life Sciences, #A300-287A, RRID: AB_263407], actin [Sigma, #A1978, RRID: AB_476692]) or 5% milk prepared in PBS-T (BRD4 [Cell Signaling Technology, #13440, RRID: AB_2687578]; BRD3 [Cell Signaling Technology, #94032]; BRD2 [Cell Signaling Technology, #5848, RRID: AB_10835146]). .. The membranes were then washed with PBS-T and treated with secondary HRP-conjugated antibodies 1:2000 in 3% BSA (p53, HDM2 membranes: anti-mouse IgG, BioRad, #1706516, RRID:AB_2921252; p21, HDMX membranes: anti-rabbit IgG, Cell Signaling Technology, #7074, RRID:AB_2099233) or in 5% milk prepared in PBS-T (BRD2-4 membranes; anti-rabbit IgG, Cell Signaling Technology, #7074, RRID:AB_2099233) for 90 min at room temperature.

    Incubation:

    Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
    Article Snippet: For the proteasome inhibition experiment, SJSA-X cells were cultured, treated with TAPTAC1, and processed as above except that bortezomib (50 nM) was added and assessment time points were at 4, 8, and 24 h and 3–8% TrisAcetate SDS-PAGE gels (Invitrogen) were used for electrophoresis. .. The membranes were blocked in a solution of 5% milk prepared in PBS-T (Phosphate Buffered Saline, 0.01% Tween) and incubated with primary antibodies overnight at 4 ◦ C, 1:1000 in 3% BSA (p53 [Millipore, #OP43-100UG, RRID: AB_10682938], p21 [Cell Signaling Technology, #2947, RRID: AB_823586], HDM2 [Millipore, #OP46-100UG, RRID: AB_10681293], HDMX [Fortis Life Sciences, #A300-287A, RRID: AB_263407], actin [Sigma, #A1978, RRID: AB_476692]) or 5% milk prepared in PBS-T (BRD4 [Cell Signaling Technology, #13440, RRID: AB_2687578]; BRD3 [Cell Signaling Technology, #94032]; BRD2 [Cell Signaling Technology, #5848, RRID: AB_10835146]). .. The membranes were then washed with PBS-T and treated with secondary HRP-conjugated antibodies 1:2000 in 3% BSA (p53, HDM2 membranes: anti-mouse IgG, BioRad, #1706516, RRID:AB_2921252; p21, HDMX membranes: anti-rabbit IgG, Cell Signaling Technology, #7074, RRID:AB_2099233) or in 5% milk prepared in PBS-T (BRD2-4 membranes; anti-rabbit IgG, Cell Signaling Technology, #7074, RRID:AB_2099233) for 90 min at room temperature.

    Cell-Signaling:

    Article Title: High-throughput in silico screen uncovers key regulators of 3D genome architecture
    Article Snippet: .. The primary antibody used for IP included: FLAG (Sigma-Aldrich, F1804), CTCF (Diagenode, C15410210), RAD21 (abcam, ab992), SMC1 (ThermoFisher, A300-055A), SMC3 (abcam, ab9263), STAG1 (abcam, ab4457), STAG2 (abcam, ab4464), BRD2 (Cell Signaling Technology Inc, # 5848), BRD4 (GeneTex, Inc, GTX130586), Goat IgG Isotype Control (ThermoFisher, 31245), Mouse IgG Isotype Control (ThermoFisher, 10400C), Rabbit IgG Isotype Control (ThermoFisher, 02-6102). .. 25 μL of Dynabeads Protein G Beads (ThermoFisher, 10004D) (for antibodies from mouse and goat) or Dynabeads Protein A Beads (ThermoFisher, 10002D) (for antibodies from rabbit) were resuspended by vortexing for 30 seconds and added to the antibody-protein complex.

    Control:

    Article Title: High-throughput in silico screen uncovers key regulators of 3D genome architecture
    Article Snippet: .. The primary antibody used for IP included: FLAG (Sigma-Aldrich, F1804), CTCF (Diagenode, C15410210), RAD21 (abcam, ab992), SMC1 (ThermoFisher, A300-055A), SMC3 (abcam, ab9263), STAG1 (abcam, ab4457), STAG2 (abcam, ab4464), BRD2 (Cell Signaling Technology Inc, # 5848), BRD4 (GeneTex, Inc, GTX130586), Goat IgG Isotype Control (ThermoFisher, 31245), Mouse IgG Isotype Control (ThermoFisher, 10400C), Rabbit IgG Isotype Control (ThermoFisher, 02-6102). .. 25 μL of Dynabeads Protein G Beads (ThermoFisher, 10004D) (for antibodies from mouse and goat) or Dynabeads Protein A Beads (ThermoFisher, 10002D) (for antibodies from rabbit) were resuspended by vortexing for 30 seconds and added to the antibody-protein complex.

    Chromatin Immunoprecipitation:




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    Image Search Results


    Serum levels of reproductive hormones in mice. ( A ) Serum AMH concentration; ( B ) serum E2 concentration; ( C ) serum FSH concentration, n = 10. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, no significant difference.

    Journal: International Journal of Molecular Sciences

    Article Title: Establishment of an Autoimmune Premature Ovarian Insufficiency Mouse Model with Proteomic Analyses: An Exploratory Study

    doi: 10.3390/ijms27104270

    Figure Lengend Snippet: Serum levels of reproductive hormones in mice. ( A ) Serum AMH concentration; ( B ) serum E2 concentration; ( C ) serum FSH concentration, n = 10. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, no significant difference.

    Article Snippet: The ELISA kits used were as follows: FSH (RK04237, ABclonal, Shanghai, China), AMH (RK09261, ABclonal, China), and E2 (RK00651, ABclonal, China).

    Techniques: Concentration Assay