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Spring Bioscience braf v600e antibody clone ve1
Braf V600e Antibody Clone Ve1, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

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Article Title: Co‐occurrence of histone H3 K27M and BRAF V600E mutations in paediatric midline grade I ganglioglioma
Article Snippet: Antibodies against the following proteins were used: glial fibrillary acidic protein (GFAP) (1:200, 6F2, Dako Denmark A/S, Glostrup, Denmark), CD34 (1:40, QBEnd‐10, Dako, Denmark A/S, Glostrup, Denmark), chromogranin A (1:200, LK2H10, Diagnostic BioSystems, Pleasanton, USA), p53 (1:5000, DO‐1, Santa Cruz Biotechnology, Dallas, USA), ATRX (1:200, polyclonal, Sigma Aldrich, St. Louis, MO), BRAF V600E (1:100, VE1, Spring Bioscience, Pleasanton, USA), H3K27M (1:1000, ABE419, EMD Millipore, Billerica, USA) and H3K27me3 (1:1250, C15410195, Diagenode, Seraing, Belgium).

Article Title: Acquired resistance to BRAFi reverses senescence-like phenotype in mutant BRAF melanoma
Article Snippet: Immunodetections were performed using antibodies raised against pAKT (Ser 473) (D9E, 1/500), AKT (40D4, 1/1000), cyclin D1 (92G2, 1/1000), pRB (Ser 608) (D10F2, 1/1000) and p21 (Waf1/Cip1) (12D1, 1/1000) (all from Cell Signaling Technology, Danvers, MA, USA), pERK (Tyr 204) (E-4, 1/1000), ERK2 (C-14, 1/2000) and p53 (DO-1, 1/200) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), and β-actin (C4, 1/5000) (from Millipore, Temecula, CA, USA) and BRAF V600E (clone VE1) (from Spring Bioscience Pleasanton, CA, USA).

Article Title: Evaluation of a Rapid, Fully Automated Platform for Detection of BRAF and NRAS Mutations in Melanoma.
Article Snippet: The monoclonal antibodies N-Ras (Q61R) (clone SP174, Spring Bioscience, Pleasanton, CA, USA) and BRAF V600E (clone VE1, Spring Bioscience) were used at a dilution of 1:100.

Article Title: Encapsulated Papillary Thyroid Tumor with Delicate Nuclear Changes and a KRAS Mutation as a Possible Novel Subtype of Borderline Tumor
Article Snippet: Immunohistochemical analysis demonstrated that the adenomatous nodule-like lesion, papillary-patterned lesion with equivocal PTC-type nuclear features, and the surrounding thyroid tissue were all negative for cytokeratin 19 (clone b170, Novocastra, Newcastle upon Tyne, UK) ( ) and BRAF V600E (clone VE1, Spring Bioscience, Pleasanton, CA, USA) ( ).

Article Title: BRAF V600E Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts
Article Snippet: The NKI-AVL Core Facility Molecular Pathology & Biobanking (CFMPB) provided the NKI-AVL Biobank patient material and performed the BRAF V600E (VE1, Spring Bioscience) staining according to the manufacturer’s protocol.

Immunohistochemical staining:

Article Title: Hairy cell leukemia presenting as a palpable breast mass
Article Snippet: .. Molecular Table 1 Markers evaluated in immunohistochemical analysis Antigen Antibody clone Sourcea Titer Retrieval Detection system CD3 SP7 Lab Vision 1:300 TUFb/steamer Rabbit Power Vision (Leica) CD5 4C7 Leica 1:400 HIER pH 9c Bond III immunostainerd Annexin A1 MRQ-3 Cell Marque 1:100 EDTA/steamer Mouse Power Vision (Leica) CD103 EPR4166 [2] Epitomics 1:3,000 EDTA/steamer Rabbit Power Vision CD20 L26 Dako 1:500 EDTA (Ventana) Ultraview Universal DAB Detection (Ventana immunostainer) TRAP 26E5 Lab Vision 1:40 ETDA/steamer Mouse Power Vision DBA.44 DBA.44 Dako 1:50 TUF/steamer Mouse Power Vision CD25 4C9 Vector 1:200 EDTA/steamer Mouse Power Vision CD123 9F5 BD Biosciences 1:25 EDTA/steamer Mouse Power Vision Cyclin D1 SP4 Thermo Scientific 1:30 HIER pH 9 Bond III immunostainer CD10 56C6 Leica 1:80 HIER pH 9 Bond III immunostainer CD43 Leu-22 BD Biosciences 1:10,000 EDTA/steamer Mouse Power Vision CD23 1B12 Cell Marque 1:50 Pressure cooker/Dako target retrieval Mouse Power Vision MPO Rabbit poly Dako 1:25,000 None Rabbit Power Vision Keratin AE1/AE3 Dako 1:200 EDTA/steamer Mouse Power Vision CD34 QBend-10 Beckman Coulter 1:70 Pressure cooker/citrate pH 6 Mouse Power Vision S100 Rabbit poly Dako 1:1,000 None Envision+ (rabbit, DAB)e Ki67 MIB-1 Dako 1:500 HIER pH 9 Bond III immunostainer BRAF V600E VE1 Spring Bioscience 1:60 HIER pH 9 Bond III immunostainer ER SP1 Thermo Scientific 1:40 Citrate/pressure cooker Envision+ PR PgR636 Dako 1:150 Citrate/pressure cooker Envision+ HER2neu SP3 Thermo Scientific 1:50 Citrate/pressure cooker Envision+ EDTA ethylenediaminetetraacetic acid, pH 8.0; HIER heat-induced epitope retrieval a Lab Vision Corporation (Fremont, CA), Leica Biosystems, Inc. (Buffalo Grove, IL), Cell Marque (Rocklin, CA), Epitomics (Burlingame, CA), Dako (Carpinteria, CA), Vector Laboratories, Inc. (Burlingame, CA), BD Biosciences (San Jose, CA), Thermo Scientific (Waltham, MA), Beckman Coulter, Inc. (Miami, FL), Spring Bioscience (Pleasanton, CA), Ventana Medical Systems (Tucson, AZ) b Target Unmasking Fluid, Life Technologies (Carlsbad, CA) c Bond reagents, Leica Biosystems, Inc. d Bond Polymer Refine DAB Detection e Dako analysis revealed the presence of the BRAF V600E mutation as well as a clonal immunoglobulin heavy chain gene rearrangement. ..

Polymer:

Article Title: Hairy cell leukemia presenting as a palpable breast mass
Article Snippet: .. Molecular Table 1 Markers evaluated in immunohistochemical analysis Antigen Antibody clone Sourcea Titer Retrieval Detection system CD3 SP7 Lab Vision 1:300 TUFb/steamer Rabbit Power Vision (Leica) CD5 4C7 Leica 1:400 HIER pH 9c Bond III immunostainerd Annexin A1 MRQ-3 Cell Marque 1:100 EDTA/steamer Mouse Power Vision (Leica) CD103 EPR4166 [2] Epitomics 1:3,000 EDTA/steamer Rabbit Power Vision CD20 L26 Dako 1:500 EDTA (Ventana) Ultraview Universal DAB Detection (Ventana immunostainer) TRAP 26E5 Lab Vision 1:40 ETDA/steamer Mouse Power Vision DBA.44 DBA.44 Dako 1:50 TUF/steamer Mouse Power Vision CD25 4C9 Vector 1:200 EDTA/steamer Mouse Power Vision CD123 9F5 BD Biosciences 1:25 EDTA/steamer Mouse Power Vision Cyclin D1 SP4 Thermo Scientific 1:30 HIER pH 9 Bond III immunostainer CD10 56C6 Leica 1:80 HIER pH 9 Bond III immunostainer CD43 Leu-22 BD Biosciences 1:10,000 EDTA/steamer Mouse Power Vision CD23 1B12 Cell Marque 1:50 Pressure cooker/Dako target retrieval Mouse Power Vision MPO Rabbit poly Dako 1:25,000 None Rabbit Power Vision Keratin AE1/AE3 Dako 1:200 EDTA/steamer Mouse Power Vision CD34 QBend-10 Beckman Coulter 1:70 Pressure cooker/citrate pH 6 Mouse Power Vision S100 Rabbit poly Dako 1:1,000 None Envision+ (rabbit, DAB)e Ki67 MIB-1 Dako 1:500 HIER pH 9 Bond III immunostainer BRAF V600E VE1 Spring Bioscience 1:60 HIER pH 9 Bond III immunostainer ER SP1 Thermo Scientific 1:40 Citrate/pressure cooker Envision+ PR PgR636 Dako 1:150 Citrate/pressure cooker Envision+ HER2neu SP3 Thermo Scientific 1:50 Citrate/pressure cooker Envision+ EDTA ethylenediaminetetraacetic acid, pH 8.0; HIER heat-induced epitope retrieval a Lab Vision Corporation (Fremont, CA), Leica Biosystems, Inc. (Buffalo Grove, IL), Cell Marque (Rocklin, CA), Epitomics (Burlingame, CA), Dako (Carpinteria, CA), Vector Laboratories, Inc. (Burlingame, CA), BD Biosciences (San Jose, CA), Thermo Scientific (Waltham, MA), Beckman Coulter, Inc. (Miami, FL), Spring Bioscience (Pleasanton, CA), Ventana Medical Systems (Tucson, AZ) b Target Unmasking Fluid, Life Technologies (Carlsbad, CA) c Bond reagents, Leica Biosystems, Inc. d Bond Polymer Refine DAB Detection e Dako analysis revealed the presence of the BRAF V600E mutation as well as a clonal immunoglobulin heavy chain gene rearrangement. ..

Mutagenesis:

Article Title: Hairy cell leukemia presenting as a palpable breast mass
Article Snippet: .. Molecular Table 1 Markers evaluated in immunohistochemical analysis Antigen Antibody clone Sourcea Titer Retrieval Detection system CD3 SP7 Lab Vision 1:300 TUFb/steamer Rabbit Power Vision (Leica) CD5 4C7 Leica 1:400 HIER pH 9c Bond III immunostainerd Annexin A1 MRQ-3 Cell Marque 1:100 EDTA/steamer Mouse Power Vision (Leica) CD103 EPR4166 [2] Epitomics 1:3,000 EDTA/steamer Rabbit Power Vision CD20 L26 Dako 1:500 EDTA (Ventana) Ultraview Universal DAB Detection (Ventana immunostainer) TRAP 26E5 Lab Vision 1:40 ETDA/steamer Mouse Power Vision DBA.44 DBA.44 Dako 1:50 TUF/steamer Mouse Power Vision CD25 4C9 Vector 1:200 EDTA/steamer Mouse Power Vision CD123 9F5 BD Biosciences 1:25 EDTA/steamer Mouse Power Vision Cyclin D1 SP4 Thermo Scientific 1:30 HIER pH 9 Bond III immunostainer CD10 56C6 Leica 1:80 HIER pH 9 Bond III immunostainer CD43 Leu-22 BD Biosciences 1:10,000 EDTA/steamer Mouse Power Vision CD23 1B12 Cell Marque 1:50 Pressure cooker/Dako target retrieval Mouse Power Vision MPO Rabbit poly Dako 1:25,000 None Rabbit Power Vision Keratin AE1/AE3 Dako 1:200 EDTA/steamer Mouse Power Vision CD34 QBend-10 Beckman Coulter 1:70 Pressure cooker/citrate pH 6 Mouse Power Vision S100 Rabbit poly Dako 1:1,000 None Envision+ (rabbit, DAB)e Ki67 MIB-1 Dako 1:500 HIER pH 9 Bond III immunostainer BRAF V600E VE1 Spring Bioscience 1:60 HIER pH 9 Bond III immunostainer ER SP1 Thermo Scientific 1:40 Citrate/pressure cooker Envision+ PR PgR636 Dako 1:150 Citrate/pressure cooker Envision+ HER2neu SP3 Thermo Scientific 1:50 Citrate/pressure cooker Envision+ EDTA ethylenediaminetetraacetic acid, pH 8.0; HIER heat-induced epitope retrieval a Lab Vision Corporation (Fremont, CA), Leica Biosystems, Inc. (Buffalo Grove, IL), Cell Marque (Rocklin, CA), Epitomics (Burlingame, CA), Dako (Carpinteria, CA), Vector Laboratories, Inc. (Burlingame, CA), BD Biosciences (San Jose, CA), Thermo Scientific (Waltham, MA), Beckman Coulter, Inc. (Miami, FL), Spring Bioscience (Pleasanton, CA), Ventana Medical Systems (Tucson, AZ) b Target Unmasking Fluid, Life Technologies (Carlsbad, CA) c Bond reagents, Leica Biosystems, Inc. d Bond Polymer Refine DAB Detection e Dako analysis revealed the presence of the BRAF V600E mutation as well as a clonal immunoglobulin heavy chain gene rearrangement. ..



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(a) Chromatogram: BRAF <t>V600E</t> -mutated PTC. (b) PTC scored 8 for <t>VE1;</t> FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases
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(a) Chromatogram: BRAF <t>V600E</t> -mutated PTC. (b) PTC scored 8 for <t>VE1;</t> FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases
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Image Search Results


Demographics (M male, F female, NS non-specified, IHC Immunohistochemistry, NGS next-generation sequencing, PCR polymerase chain reaction, BID twice a day, QD once a day, CR complete response, NCR near-complete response, PR partial response, SD stable disease, N no, TTT treatment).

Journal: Cancers

Article Title: Anti-MAPK Targeted Therapy for Ameloblastoma: Case Report with a Systematic Review

doi: 10.3390/cancers16122174

Figure Lengend Snippet: Demographics (M male, F female, NS non-specified, IHC Immunohistochemistry, NGS next-generation sequencing, PCR polymerase chain reaction, BID twice a day, QD once a day, CR complete response, NCR near-complete response, PR partial response, SD stable disease, N no, TTT treatment).

Article Snippet: The histopathologic examination revealed ameloblastoma ( A), with positive immunohistostaining for BRAF V600E (clone VE1, Ventana Medical Systems, B).

Techniques: Immunohistochemistry, Next-Generation Sequencing, Polymerase Chain Reaction

(a) Chromatogram: BRAF V600E -mutated PTC. (b) PTC scored 8 for VE1; FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases

Journal: Indian Journal of Endocrinology and Metabolism

Article Title: Elucidating the Prognostic Role of BRAF V600E and the Activation Status of the Downstream MAPK Pathway in PTC: A Study from a Tertiary Centre in India

doi: 10.4103/ijem.ijem_235_23

Figure Lengend Snippet: (a) Chromatogram: BRAF V600E -mutated PTC. (b) PTC scored 8 for VE1; FA (inset) scored 6 (400×). (c) ROC curve: BRAF V600E IHC. (d) PTC: pMEK1/2 IHC score III (400×). (e) NIFTP: patchy pERK1/2 immunopositivity (400×). (f) DFS curves: BRAF V600E -mutated and BRAF V600E -wt cases

Article Snippet: Five-micron-thick formalin-fixed paraffin-embedded representative sections were stained for BRAF V600E (clone VE1, 1:50; Spring Bioscience) by using the Ventana automated immunohistochemical stainer according to the manufacturer’s specifications (Ventana Medical Systems, Tucson, AZ).

Techniques: