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paper n a bosc 23 retroviral packaging cells atcc cat  (ATCC)


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    ATCC paper n a bosc 23 retroviral packaging cells atcc cat
    Paper N A Bosc 23 Retroviral Packaging Cells Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bosc+23+cells/Microbotryum+violaceum+(Persoon)+Deml+et+Oberwinkler%2C+teleomorph/pm36800289-424-117-124
    Average 92 stars, based on 8 article reviews
    paper n a bosc 23 retroviral packaging cells atcc cat - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    shRNA:

    Article Title: Endothelial cells create a stem cell niche in glioblastoma by providing Notch ligands that nurture self-renewal of cancer stem-like cells
    Article Snippet: .. Lentivirus Production and shRNA Transduction Lentiviruses were produced by transfecting BOSC 23 cells (ATCC, catalog #CRL-11270) with 16 μg total of pSicoR (Addgene, catalog #11579, Cambridge, MA), psPAX2 (Addgene, catalog #12260), and PMD.2G (Addgene, catalog #12259) plasmids with a ratio of 5: 3.5: 1.75 in a 10-cm dish, using lipofectamine 2000 (Invitrogen) ( 30 ). ..

    Transduction:

    Article Title: Endothelial cells create a stem cell niche in glioblastoma by providing Notch ligands that nurture self-renewal of cancer stem-like cells
    Article Snippet: .. Lentivirus Production and shRNA Transduction Lentiviruses were produced by transfecting BOSC 23 cells (ATCC, catalog #CRL-11270) with 16 μg total of pSicoR (Addgene, catalog #11579, Cambridge, MA), psPAX2 (Addgene, catalog #12260), and PMD.2G (Addgene, catalog #12259) plasmids with a ratio of 5: 3.5: 1.75 in a 10-cm dish, using lipofectamine 2000 (Invitrogen) ( 30 ). ..

    Produced:

    Article Title: Endothelial cells create a stem cell niche in glioblastoma by providing Notch ligands that nurture self-renewal of cancer stem-like cells
    Article Snippet: .. Lentivirus Production and shRNA Transduction Lentiviruses were produced by transfecting BOSC 23 cells (ATCC, catalog #CRL-11270) with 16 μg total of pSicoR (Addgene, catalog #11579, Cambridge, MA), psPAX2 (Addgene, catalog #12260), and PMD.2G (Addgene, catalog #12259) plasmids with a ratio of 5: 3.5: 1.75 in a 10-cm dish, using lipofectamine 2000 (Invitrogen) ( 30 ). ..

    Article Title: Endothelial cells create a stem cell niche in glioblastoma by providing Notch ligands that nurture self-renewal of cancer stem-like cells
    Article Snippet: .. Lentiviruses were produced by transfecting BOSC 23 cells (ATCC, catalog #CRL-11270) with 16 μg total of pSicoR (Addgene, catalog #11579, Cambridge, MA), psPAX2 (Addgene, catalog #12260), and PMD.2G (Addgene, catalog #12259) plasmids with a ratio of 5: 3.5: 1.75 in a 10-cm dish, using lipofectamine 2000 (Invitrogen) ( 30 ). ..

    other:


    Transfection:

    Article Title: Ancestral acetylcholine receptor β-subunit forms homopentamers that prime before opening spontaneously
    Article Snippet: .. Combinations of human and ancestral subunit cDNAs were transfected into BOSC 23 cells (Pear et al., 1993), originally from ATCC (CRL11270), but provided by Steven M. Sine (Mayo Clinic) (RRID:CVCL_4401). .. Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Corning) containing 10% (vol/vol) fetal bovine serum (Gibco) at 37°C, until they reached 50–70% confluency.

    Article Title: Derepression may masquerade as activation in ligand-gated ion channels
    Article Snippet: .. cDNAs encoding human and ancestral subunits were transfected into BOSC 23 cells (CVCL_4401; ATCC number: CRL-11270). ..

    Mutagenesis:

    Article Title: A conserved arginine with non-conserved function is a key determinant of agonist selectivity in α7 nicotinic ACh receptors.
    Article Snippet: The decay from the peak to steady-state inward current response was fitted with a biexponential expression using GraphPad software Version 5: f(t) = Af−t/τf + As−t/τs + C, where f(t) is the macroscopic current amplitude at any given time, Af is the magnitude of the fast decay component, corresponding to fast desensitisation and characterised by time constant τf, As is the magnitude of the slower component corresponding to the slow desensitisation process characterised by time constant τs and C is the steady-state current. .. Single-channel currents were recorded from BOSC 23 cells (ATCC Cat. No. CRL-11270, RRID:CVCL_4401) co-transfected with α7 subunit (wild-type or mutant) cDNA and the chaperone Ric-3 cDNA (1:4) by calcium phosphate precipitation. ..

    Cell Culture:

    Article Title: Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160Δc BaL
    Article Snippet: PBMCs were isolated by Ficoll-Paque (GE Healthcare) density gradient centrifugation. .. HEK293T/17 (293T; American Type Culture Collection; CRL-11268) cells and BOSC.23 cells (American Type Culture Collection; CRL-11270) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 1mM sodium pyruvate (Gibco), 1% antibiotic-antimycotic (Gibco) and 10% fetal bovine serum (FBS; HyClone, Thermo Scientific) at 37°C and 5% CO 2 . .. The recombinant murine B cell leukemia cell line 70z/3 ( Paige et al., 1978 ) was grown in RPMI 1640 (Gibco) supplemented with 1mM sodium pyruvate, 2mM L-glutamine (Gibco), 10mM HEPES buffer solution (Gibco), 0.055mM β-Mercaptoethanol (Gibco), 1% antibiotic-antimycotic and 10% FBS (HyClone, Thermo Scientific).

    Modification:

    Article Title: Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160Δc BaL
    Article Snippet: PBMCs were isolated by Ficoll-Paque (GE Healthcare) density gradient centrifugation. .. HEK293T/17 (293T; American Type Culture Collection; CRL-11268) cells and BOSC.23 cells (American Type Culture Collection; CRL-11270) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 1mM sodium pyruvate (Gibco), 1% antibiotic-antimycotic (Gibco) and 10% fetal bovine serum (FBS; HyClone, Thermo Scientific) at 37°C and 5% CO 2 . .. The recombinant murine B cell leukemia cell line 70z/3 ( Paige et al., 1978 ) was grown in RPMI 1640 (Gibco) supplemented with 1mM sodium pyruvate, 2mM L-glutamine (Gibco), 10mM HEPES buffer solution (Gibco), 0.055mM β-Mercaptoethanol (Gibco), 1% antibiotic-antimycotic and 10% FBS (HyClone, Thermo Scientific).



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    Expression and agonist-dependent single-channel activity of single and pairwise mutant receptors. A , radio-labeled epibatidine binding to intact <t>Bosc</t> <t>23</t> cells–expressing WT and mutant receptors was measured as described in . Results from two independent transfections, each in duplicate, are shown, with control binding defined as that measured for the WT receptor in the presence of epibatidine alone. Net cell surface binding is the difference between binding in the presence of epibatidine alone ( open bars ) and that in the presence of ACh ( gray bars ); binding in the presence of ACh corresponds to intracellular binding sites. B , single-channel recordings from the same of patch of cell membrane before and after addition of nicotine (final concentration 100 nM) to the bath solution for the indicated receptors. Traces are representative of three experiments per receptor. The electrical artifact during nicotine addition is removed. The cell attached patch configuration was maintained throughout with a membrane potential of −70 mV and display bandwidth of 2 kHz.
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    Expression and agonist-dependent single-channel activity of single and pairwise mutant receptors. A , radio-labeled epibatidine binding to intact <t>Bosc</t> <t>23</t> cells–expressing WT and mutant receptors was measured as described in . Results from two independent transfections, each in duplicate, are shown, with control binding defined as that measured for the WT receptor in the presence of epibatidine alone. Net cell surface binding is the difference between binding in the presence of epibatidine alone ( open bars ) and that in the presence of ACh ( gray bars ); binding in the presence of ACh corresponds to intracellular binding sites. B , single-channel recordings from the same of patch of cell membrane before and after addition of nicotine (final concentration 100 nM) to the bath solution for the indicated receptors. Traces are representative of three experiments per receptor. The electrical artifact during nicotine addition is removed. The cell attached patch configuration was maintained throughout with a membrane potential of −70 mV and display bandwidth of 2 kHz.
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    Expression and agonist-dependent single-channel activity of single and pairwise mutant receptors. A , radio-labeled epibatidine binding to intact <t>Bosc</t> <t>23</t> cells–expressing WT and mutant receptors was measured as described in . Results from two independent transfections, each in duplicate, are shown, with control binding defined as that measured for the WT receptor in the presence of epibatidine alone. Net cell surface binding is the difference between binding in the presence of epibatidine alone ( open bars ) and that in the presence of ACh ( gray bars ); binding in the presence of ACh corresponds to intracellular binding sites. B , single-channel recordings from the same of patch of cell membrane before and after addition of nicotine (final concentration 100 nM) to the bath solution for the indicated receptors. Traces are representative of three experiments per receptor. The electrical artifact during nicotine addition is removed. The cell attached patch configuration was maintained throughout with a membrane potential of −70 mV and display bandwidth of 2 kHz.
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    Expression and agonist-dependent single-channel activity of single and pairwise mutant receptors. A , radio-labeled epibatidine binding to intact Bosc 23 cells–expressing WT and mutant receptors was measured as described in . Results from two independent transfections, each in duplicate, are shown, with control binding defined as that measured for the WT receptor in the presence of epibatidine alone. Net cell surface binding is the difference between binding in the presence of epibatidine alone ( open bars ) and that in the presence of ACh ( gray bars ); binding in the presence of ACh corresponds to intracellular binding sites. B , single-channel recordings from the same of patch of cell membrane before and after addition of nicotine (final concentration 100 nM) to the bath solution for the indicated receptors. Traces are representative of three experiments per receptor. The electrical artifact during nicotine addition is removed. The cell attached patch configuration was maintained throughout with a membrane potential of −70 mV and display bandwidth of 2 kHz.

    Journal: The Journal of Biological Chemistry

    Article Title: Pathogenic residue insertion in neuronal nicotinic receptor alters intra- and inter-subunit interactions that tune channel gating

    doi: 10.1016/j.jbc.2024.107266

    Figure Lengend Snippet: Expression and agonist-dependent single-channel activity of single and pairwise mutant receptors. A , radio-labeled epibatidine binding to intact Bosc 23 cells–expressing WT and mutant receptors was measured as described in . Results from two independent transfections, each in duplicate, are shown, with control binding defined as that measured for the WT receptor in the presence of epibatidine alone. Net cell surface binding is the difference between binding in the presence of epibatidine alone ( open bars ) and that in the presence of ACh ( gray bars ); binding in the presence of ACh corresponds to intracellular binding sites. B , single-channel recordings from the same of patch of cell membrane before and after addition of nicotine (final concentration 100 nM) to the bath solution for the indicated receptors. Traces are representative of three experiments per receptor. The electrical artifact during nicotine addition is removed. The cell attached patch configuration was maintained throughout with a membrane potential of −70 mV and display bandwidth of 2 kHz.

    Article Snippet: Bosc 23 cells, an HEK293-derived cell line (CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045) , were used to express WT and mutant receptors.

    Techniques: Expressing, Activity Assay, Mutagenesis, Labeling, Binding Assay, Transfection, Control, Membrane, Concentration Assay