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boltzmann sigmoidal fitted curves  (GraphPad Software Inc)


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    GraphPad Software Inc boltzmann sigmoidal fitted curves
    Boltzmann Sigmoidal Fitted Curves, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+fitting/boltzmann+sigmoidal/pmc11127010__pnas__2318874121__sapp-142-14-24
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoidal fitted curves - by Bioz Stars, 2026-10
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    Article Title: Structural basis for CCR6 modulation by allosteric antagonists
    Article Snippet: Melting temperature (T m ) is defined as the temperature at which 50% of the protein was denatured based on Boltzmann sigmoidal fitting using GraphPad Prism v9.5.1.

    Article Title: Crystal structures of human NSDHL and development of its novel inhibitor with the potential to suppress EGFR activity
    Article Snippet: Midpoint melting temperature (T m ) values were calculated using Boltzmann sigmoidal fitting in GraphPad Prism five (GraphPad software).

    Article Title: Crystal structures of human NSDHL and development of its novel inhibitor with the potential to suppress EGFR activity.
    Article Snippet: NAD(P)-dependent steroid dehydrogenase-like (NSDHL), an essential enzyme in human cholesterol synthesis and a regulator of epidermal growth factor receptor (EGFR) trafficking pathways, has attracted interest as a therapeutic target due to its crucial relevance to cholesterol-related diseases and carcinomas.. However, the development of pharmacological agents for targeting NSDHL has been hindered by the absence of the atomic details of NSDHL.. In this study, we reported two X-ray crystal structures of human NSDHL, which revealed a detailed description of the coenzyme-binding site and the unique conformational change upon the binding of a coenzyme.

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    Article Title: Identification of an ATP-sensitive potassium channel in mitochondria.
    Article Snippet: .. The solid lines represent the best fits of the data using a Boltzmann sigmoidal fitting within the GraphPad Prism software. ..



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    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted <t>Boltzmann</t> functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.
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    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted <t>Boltzmann</t> functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.
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    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted <t>Boltzmann</t> functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.
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    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted <t>Boltzmann</t> functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.
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    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted <t>Boltzmann</t> functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.
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    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted <t>Boltzmann</t> functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.
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    Image Search Results


    a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted Boltzmann functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.

    Journal: Communications Biology

    Article Title: Stability convergence in natural antibodies with ultra-long hypervariable loops

    doi: 10.1038/s42003-025-08036-5

    Figure Lengend Snippet: a Structure of a bovine Fab NC-Cow1 (PDB:6OO0) with an ultra-long CDR-H3 (gray color). Schematic figure and ulCDR sequence alignment of the Fab variants in this work. b Non-reducing SDS-PAGE of cell supernatants with secreted Fab fragments and a purified NC-Cow1 wt reference. c Chromatograms and molecular mass in SEC-MALS. d Mean values ( n = 2) of molecular masses calculated from MALS. e Exemplary melting curves in SUPR-DSF. f Apparent onset temperatures of unfolding T on and melting temperatures T m obtained from SUPR-DSF measurements. Mean values ( n = 3). g Slope factors of fitted Boltzmann functions obtained from SUPR-DSF melting curves. A smaller slope factor indicates a steeper transition (more cooperative unfolding). Mean values ( n = 3; SD shown in Table ). h Apparent hydrodynamic radii R h from DLS. Mean values ( n = 3) with SD. i Apparent hydrodynamic radii R h during heating ramp with DLS. j Aggregation onset temperatures T agg obtained from heat-ramped DLS. Mean values ( n = 3) with SD.

    Article Snippet: For comparing the slopes of the melting curves, a sigmoidal Boltzmann fit was performed using GraphPad Prism version 8.0.1 for Windows, GraphPad Software, Boston, Massachusetts USA, www.graphpad.com .

    Techniques: Sequencing, SDS Page, Purification