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ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 <t>antagonist,</t> <t>Boc-MLF</t> (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.
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ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 <t>antagonist,</t> <t>Boc-MLF</t> (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.
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ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 <t>antagonist,</t> <t>Boc-MLF</t> (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.
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ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 <t>antagonist,</t> <t>Boc-MLF</t> (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.
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ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 <t>antagonist,</t> <t>Boc-MLF</t> (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.
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ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.

Journal: bioRxiv

Article Title: The miR-183/96/182 Cluster Regulates Trigeminal Ganglion Sensory Neurons’ Response to Pseudomonas aeruginosa Infection

doi: 10.64898/2026.03.30.715374

Figure Lengend Snippet: ELISA assays in the supernatant of the axon chambers. NTC: no-treatment control; a-TLR+PA: the axon chamber pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA: the axon chamber pre-treated with Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: the axon chamber pre-treated with both LPS-RS Ultrapure (20 µg/mL) and Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.

Article Snippet: To test this hypothesis, we pre-treated the neurites in the axon chambers with a TLR4-specific antagonist, TLR4-RS (InVivoGen), and/or a FPR1-specific antagonist, Boc-MLF (TOCRIS) 2 hours before PA infection.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Infection, Comparison

ELISA assays in the cell lysates of the soma chambers. NTC: no treatment control; a-TLR+PA: axon chambers pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA:, the axon chambers pre-treated with Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: axon chambers pre-treated with LPS-RS Ultrapure (20 µg/mL) and Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.

Journal: bioRxiv

Article Title: The miR-183/96/182 Cluster Regulates Trigeminal Ganglion Sensory Neurons’ Response to Pseudomonas aeruginosa Infection

doi: 10.64898/2026.03.30.715374

Figure Lengend Snippet: ELISA assays in the cell lysates of the soma chambers. NTC: no treatment control; a-TLR+PA: axon chambers pretreated with TLR4-specific antagonist, LPS-RS Ultrapure (20 µg/mL) 2 hours before PA infection; a-Fpr+PA:, the axon chambers pre-treated with Fpr1 antagonist, Boc-MLF (2 µg/mL) 2 hours before PA infection; a-TLR/Fpr+PA: axon chambers pre-treated with LPS-RS Ultrapure (20 µg/mL) and Boc-MLF (2 µg/mL) 2 hours before PA infection. *: p<0.05, **: p<0.01, ***:p<0.001 by one-way ANOVA of multiple sample comparison with Bonferroni post hoc test. #: p<0.05, ##: p<0.01, ###: p<0.001 by Student’s t test.

Article Snippet: To test this hypothesis, we pre-treated the neurites in the axon chambers with a TLR4-specific antagonist, TLR4-RS (InVivoGen), and/or a FPR1-specific antagonist, Boc-MLF (TOCRIS) 2 hours before PA infection.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Infection, Comparison